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The map label for this gene is spaR [H]

Identifier: 170754678

GI number: 170754678

Start: 2058217

End: 2058747

Strand: Reverse

Name: spaR [H]

Synonym: CLD_2700

Alternate gene names: 170754678

Gene position: 2058747-2058217 (Counterclockwise)

Preceding gene: 170756166

Following gene: 170754560

Centisome position: 52.01

GC content: 32.39

Gene sequence:

>531_bases
ATGATGCCTAATATTGATGGTTTTCAGGTTTGCCAAAAGATAAGGGACAAAGTATCCTGTCCTATAATATTTTTAAGTGC
TAGACAAAGTGAGATCGATAAAATTAAAGGTCTTTCAGTAGGTGGGGATGATTATATAGTAAAGCCCTTTAGCATAAAAG
AATTAAAAGCAAGAGTTCAGGCTCATCTTAGAAGGGAAAAAAGAGTTCCTTTAAATAATAGATCTAAGATAAGCTTTGAG
AAACTTACTTTAGATATTAATAGTCGTAAAGTTCATTATGAGGATGAGGAAATACTTTTTACAGCTAAAGAATTTGATAT
AGTAGAACTTTTAGCATTACATCCAGAGCAGGTGTTTTCTAAAGAACAAATCTATGAAAAAGTTTGGGGATATGATGCAG
AAGGGGATGCAAATACAGTAGCGGAGCATATAAAAAAGATAAGAGCAAAGTTCATAAAAAGGAATGCATCTCTTAATCAT
ATAGCTACGGTATGGGGAGTAGGTTATAGATGGGAGAAAAATAAAATATGA

Upstream 100 bases:

>100_bases
TTATTTTTAGGATGAAGGCTACGGCGTTATTACAGCTAGTAATGGTAGAGAGGCTATAGAATACTGTGATAAAGATATAG
ATATTATTTTACTTGATATA

Downstream 100 bases:

>100_bases
AAAAAGCATCCTTAAAAACACAAACTATAAGTACTTTTCTATTAATTATATTTTTAAGTATGGTATGTACAATAACTACT
ATAGGAGTTTATTTTTATAT

Product: DNA-binding response regulator

Products: NA

Alternate protein names: Subtilin biosynthesis regulatory protein spaR [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MMPNIDGFQVCQKIRDKVSCPIIFLSARQSEIDKIKGLSVGGDDYIVKPFSIKELKARVQAHLRREKRVPLNNRSKISFE
KLTLDINSRKVHYEDEEILFTAKEFDIVELLALHPEQVFSKEQIYEKVWGYDAEGDANTVAEHIKKIRAKFIKRNASLNH
IATVWGVGYRWEKNKI

Sequences:

>Translated_176_residues
MMPNIDGFQVCQKIRDKVSCPIIFLSARQSEIDKIKGLSVGGDDYIVKPFSIKELKARVQAHLRREKRVPLNNRSKISFE
KLTLDINSRKVHYEDEEILFTAKEFDIVELLALHPEQVFSKEQIYEKVWGYDAEGDANTVAEHIKKIRAKFIKRNASLNH
IATVWGVGYRWEKNKI
>Mature_176_residues
MMPNIDGFQVCQKIRDKVSCPIIFLSARQSEIDKIKGLSVGGDDYIVKPFSIKELKARVQAHLRREKRVPLNNRSKISFE
KLTLDINSRKVHYEDEEILFTAKEFDIVELLALHPEQVFSKEQIYEKVWGYDAEGDANTVAEHIKKIRAKFIKRNASLNH
IATVWGVGYRWEKNKI

Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaR may function as a regulatory protein [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789809, Length=174, Percent_Identity=33.3333333333333, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI87082012, Length=169, Percent_Identity=38.4615384615385, Blast_Score=114, Evalue=3e-27,
Organism=Escherichia coli, GI1786599, Length=174, Percent_Identity=35.632183908046, Blast_Score=113, Evalue=7e-27,
Organism=Escherichia coli, GI1790552, Length=175, Percent_Identity=36, Blast_Score=110, Evalue=4e-26,
Organism=Escherichia coli, GI1788394, Length=176, Percent_Identity=35.7954545454545, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1790860, Length=169, Percent_Identity=34.9112426035503, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1786911, Length=170, Percent_Identity=32.9411764705882, Blast_Score=107, Evalue=3e-25,
Organism=Escherichia coli, GI1786784, Length=173, Percent_Identity=34.1040462427746, Blast_Score=106, Evalue=9e-25,
Organism=Escherichia coli, GI2367329, Length=174, Percent_Identity=31.6091954022989, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1789402, Length=169, Percent_Identity=31.3609467455621, Blast_Score=91, Evalue=3e-20,
Organism=Escherichia coli, GI1787375, Length=173, Percent_Identity=35.2601156069364, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1790863, Length=176, Percent_Identity=30.6818181818182, Blast_Score=88, Evalue=3e-19,
Organism=Escherichia coli, GI145693140, Length=178, Percent_Identity=28.0898876404494, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1787229, Length=173, Percent_Identity=26.0115606936416, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 20458; Mature: 20458

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPNIDGFQVCQKIRDKVSCPIIFLSARQSEIDKIKGLSVGGDDYIVKPFSIKELKARVQ
CCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHH
AHLRREKRVPLNNRSKISFEKLTLDINSRKVHYEDEEILFTAKEFDIVELLALHPEQVFS
HHHHHHHCCCCCCCCCEEEEEEEEEECCCEEEECCCEEEEEECCCCHHHHHHHCHHHHHH
KEQIYEKVWGYDAEGDANTVAEHIKKIRAKFIKRNASLNHIATVWGVGYRWEKNKI
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCCCC
>Mature Secondary Structure
MMPNIDGFQVCQKIRDKVSCPIIFLSARQSEIDKIKGLSVGGDDYIVKPFSIKELKARVQ
CCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHH
AHLRREKRVPLNNRSKISFEKLTLDINSRKVHYEDEEILFTAKEFDIVELLALHPEQVFS
HHHHHHHCCCCCCCCCEEEEEEEEEECCCEEEECCCEEEEEECCCCHHHHHHHCHHHHHH
KEQIYEKVWGYDAEGDANTVAEHIKKIRAKFIKRNASLNHIATVWGVGYRWEKNKI
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8439156 [H]