| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is rho
Identifier: 170754540
GI number: 170754540
Start: 172026
End: 173471
Strand: Direct
Name: rho
Synonym: CLD_0652
Alternate gene names: 170754540
Gene position: 172026-173471 (Clockwise)
Preceding gene: 170757224
Following gene: 170755962
Centisome position: 4.35
GC content: 29.81
Gene sequence:
>1446_bases TTGATAAATAAGGATTATGAAAGTATGACCGTTAAAGAGTTAAGAGAAATAGCTAAAAAGCTTGATATAAAAAATATTTC TAAGTTCAAAAAAAATGAATTAATAGAAGAAATAAAAACTTCAAAGTCAACAGCCATAGAGAAAGATGGAAAAATTTTAG TTGAAAAAATATCACCTAAAATCATAAAAGGTGAAAATAATATACATAGCAAAATAACTAGAGAAAATGGTATTAATGAA GAAAAAACCAGTTCACTTATGAAGAATAACGAAGAAAATAAAGCTGAAAGGCTAAAAGAGATGATAAATGAATCTCAAAG AGCTAAGGGTGTTTTAGAGATTATAGAAAATAATAATTATGGATTTCTAAGAGGACAAAATTATTTGTCAGGCCCAGAAG ATATATATGTATCTCCATCGCAGATAAGAAGATTTAATTTAAGAACAGGTGATGAAGTTGAAGGAAAGGTTAGAATACCA AAGGATGGAGAAAAATTTAAAGCCTTAATATATGTAGAAAGGGTAAATGGAGAAAATCCTGAGAAAGCAGTAGGAAGAAA ACCTTTTGAAAGCTTAGTACCTATATATCCTAATGAAAGGATTAAACTTGAAACAAATTCACAAGATTTATCTACACGGC TAATGGATATAATATCACCTATAGGTAAAGGACAAAGAGGAATGATAGTAGCCCCTCCTAAAGCAGGTAAAACTACTCTT CTAAAAAAGATAGCCCAAAGCATTTCCCGAAATAATCCATTATGTAAACTTATAGTACTTTTAATTGATGAGAGACCAGA AGAAGTAACAGATATGCAAAGATCTATTAAAGGAGAAGTAATATACTCAACCTTTGATGAAGAGCCAGAACATCATACAA AGGTTACTTATATGGTTTTAGAAAGAGCTAAAAGAATGGTAGAGCAAGGACAGGATGTTGTTATATTATTAGATAGTATA ACAAGATTGTCTAGAGCATATAATTTAACTATTACACCAACAGGTAGGACATTATCAGGCGGATTAGATCCTGGTGCATT AGTAATGCCAAAGAAATTTTTCGGTGCTGCTAGAAACATTGAAAATGGTGGAAGTTTAACCATATTAGCTACAGCTTTAA CTGAAACAGGAAGCAGAATGGATGATATGATATTTGAGGAGTTTAAGGGTACTGGAAATATGGAAGTACATCTTAATAGA AAACTTCAAGAAAGAAGAATATTCCCTGCTTTAGATATTTATAAATCAGGTACAAGAAGGGATGATTTATTGTTTACAGA TTCTTTAGAAAAAGAAACTGCATTTAACATAAGAAAGCTTCTTTATGAGGAAAATAATGTAGAGAATGTAACTGAACAAG TTTTAAGTATATTATCTAAAACTAAGACTAATGAGGAATTTGTAAACATAATAAGCAAAATGGATATGAATAAAAATAAT AGATAG
Upstream 100 bases:
>100_bases TTTTGAAAGATTTTCTAAAATTTAACTACTCTTTTTAACCTAAGTTTTGATATTTTCCTGTTATTTAATATATATAATTT AATAGAGAGAGGGGATTTCT
Downstream 100 bases:
>100_bases AAAATAAATAAAACCTAATCTGAAAGATTAGGTTTTATACTATTTAACGTCTTCGTTAGATAGGTTGAATTTTTTCATGA ATTTATCAACTCTTCCGCCA
Product: transcription termination factor Rho
Products: NA
Alternate protein names: ATP-dependent helicase Rho [H]
Number of amino acids: Translated: 481; Mature: 481
Protein sequence:
>481_residues MINKDYESMTVKELREIAKKLDIKNISKFKKNELIEEIKTSKSTAIEKDGKILVEKISPKIIKGENNIHSKITRENGINE EKTSSLMKNNEENKAERLKEMINESQRAKGVLEIIENNNYGFLRGQNYLSGPEDIYVSPSQIRRFNLRTGDEVEGKVRIP KDGEKFKALIYVERVNGENPEKAVGRKPFESLVPIYPNERIKLETNSQDLSTRLMDIISPIGKGQRGMIVAPPKAGKTTL LKKIAQSISRNNPLCKLIVLLIDERPEEVTDMQRSIKGEVIYSTFDEEPEHHTKVTYMVLERAKRMVEQGQDVVILLDSI TRLSRAYNLTITPTGRTLSGGLDPGALVMPKKFFGAARNIENGGSLTILATALTETGSRMDDMIFEEFKGTGNMEVHLNR KLQERRIFPALDIYKSGTRRDDLLFTDSLEKETAFNIRKLLYEENNVENVTEQVLSILSKTKTNEEFVNIISKMDMNKNN R
Sequences:
>Translated_481_residues MINKDYESMTVKELREIAKKLDIKNISKFKKNELIEEIKTSKSTAIEKDGKILVEKISPKIIKGENNIHSKITRENGINE EKTSSLMKNNEENKAERLKEMINESQRAKGVLEIIENNNYGFLRGQNYLSGPEDIYVSPSQIRRFNLRTGDEVEGKVRIP KDGEKFKALIYVERVNGENPEKAVGRKPFESLVPIYPNERIKLETNSQDLSTRLMDIISPIGKGQRGMIVAPPKAGKTTL LKKIAQSISRNNPLCKLIVLLIDERPEEVTDMQRSIKGEVIYSTFDEEPEHHTKVTYMVLERAKRMVEQGQDVVILLDSI TRLSRAYNLTITPTGRTLSGGLDPGALVMPKKFFGAARNIENGGSLTILATALTETGSRMDDMIFEEFKGTGNMEVHLNR KLQERRIFPALDIYKSGTRRDDLLFTDSLEKETAFNIRKLLYEENNVENVTEQVLSILSKTKTNEEFVNIISKMDMNKNN R >Mature_481_residues MINKDYESMTVKELREIAKKLDIKNISKFKKNELIEEIKTSKSTAIEKDGKILVEKISPKIIKGENNIHSKITRENGINE EKTSSLMKNNEENKAERLKEMINESQRAKGVLEIIENNNYGFLRGQNYLSGPEDIYVSPSQIRRFNLRTGDEVEGKVRIP KDGEKFKALIYVERVNGENPEKAVGRKPFESLVPIYPNERIKLETNSQDLSTRLMDIISPIGKGQRGMIVAPPKAGKTTL LKKIAQSISRNNPLCKLIVLLIDERPEEVTDMQRSIKGEVIYSTFDEEPEHHTKVTYMVLERAKRMVEQGQDVVILLDSI TRLSRAYNLTITPTGRTLSGGLDPGALVMPKKFFGAARNIENGGSLTILATALTETGSRMDDMIFEEFKGTGNMEVHLNR KLQERRIFPALDIYKSGTRRDDLLFTDSLEKETAFNIRKLLYEENNVENVTEQVLSILSKTKTNEEFVNIISKMDMNKNN R
Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]
COG id: COG1158
COG function: function code K; Transcription termination factor
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the Rho family [H]
Homologues:
Organism=Escherichia coli, GI1790217, Length=369, Percent_Identity=55.5555555555556, Blast_Score=426, Evalue=1e-120,
Paralogues:
None
Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000194 - InterPro: IPR003593 - InterPro: IPR011129 - InterPro: IPR012340 - InterPro: IPR011112 - InterPro: IPR011113 - InterPro: IPR004665 [H]
Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]
EC number: NA
Molecular weight: Translated: 54787; Mature: 54787
Theoretical pI: Translated: 9.47; Mature: 9.47
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 3.1 %Met (Translated Protein) 3.3 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 3.1 %Met (Mature Protein) 3.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MINKDYESMTVKELREIAKKLDIKNISKFKKNELIEEIKTSKSTAIEKDGKILVEKISPK CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCEEEEECCCC IIKGENNIHSKITRENGINEEKTSSLMKNNEENKAERLKEMINESQRAKGVLEIIENNNY EEECCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCE GFLRGQNYLSGPEDIYVSPSQIRRFNLRTGDEVEGKVRIPKDGEKFKALIYVERVNGENP EEEECCCCCCCCCCEEECHHHHHEECCCCCCCCCCEEECCCCCCCEEEEEEEEECCCCCH EKAVGRKPFESLVPIYPNERIKLETNSQDLSTRLMDIISPIGKGQRGMIVAPPKAGKTTL HHHHCCCCHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHH LKKIAQSISRNNPLCKLIVLLIDERPEEVTDMQRSIKGEVIYSTFDEEPEHHTKVTYMVL HHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHH ERAKRMVEQGQDVVILLDSITRLSRAYNLTITPTGRTLSGGLDPGALVMPKKFFGAARNI HHHHHHHHCCCCEEEEHHHHHHHHHHEEEEECCCCCEECCCCCCCCEECCHHHHHHHCCC ENGGSLTILATALTETGSRMDDMIFEEFKGTGNMEVHLNRKLQERRIFPALDIYKSGTRR CCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHCCCHHHHHCCCCCC DDLLFTDSLEKETAFNIRKLLYEENNVENVTEQVLSILSKTKTNEEFVNIISKMDMNKNN CCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC R C >Mature Secondary Structure MINKDYESMTVKELREIAKKLDIKNISKFKKNELIEEIKTSKSTAIEKDGKILVEKISPK CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCEEEEECCCC IIKGENNIHSKITRENGINEEKTSSLMKNNEENKAERLKEMINESQRAKGVLEIIENNNY EEECCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCE GFLRGQNYLSGPEDIYVSPSQIRRFNLRTGDEVEGKVRIPKDGEKFKALIYVERVNGENP EEEECCCCCCCCCCEEECHHHHHEECCCCCCCCCCEEECCCCCCCEEEEEEEEECCCCCH EKAVGRKPFESLVPIYPNERIKLETNSQDLSTRLMDIISPIGKGQRGMIVAPPKAGKTTL HHHHCCCCHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHH LKKIAQSISRNNPLCKLIVLLIDERPEEVTDMQRSIKGEVIYSTFDEEPEHHTKVTYMVL HHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHH ERAKRMVEQGQDVVILLDSITRLSRAYNLTITPTGRTLSGGLDPGALVMPKKFFGAARNI HHHHHHHHCCCCEEEEHHHHHHHHHHEEEEECCCCCEECCCCCCCCEECCHHHHHHHCCC ENGGSLTILATALTETGSRMDDMIFEEFKGTGNMEVHLNRKLQERRIFPALDIYKSGTRR CCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHCCCHHHHHCCCCCC DDLLFTDSLEKETAFNIRKLLYEENNVENVTEQVLSILSKTKTNEEFVNIISKMDMNKNN CCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC R C
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8423140; 9353933; 9384377 [H]