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The map label for this gene is pflD [H]

Identifier: 170754495

GI number: 170754495

Start: 3456864

End: 3459227

Strand: Reverse

Name: pflD [H]

Synonym: CLD_1348

Alternate gene names: 170754495

Gene position: 3459227-3456864 (Counterclockwise)

Preceding gene: 170754784

Following gene: 170755914

Centisome position: 87.39

GC content: 31.3

Gene sequence:

>2364_bases
ATGATGACAGACAGAGTTAAAAAATTAAGAGAAGAAAGTTTAAAAGCTGTACCACGTATTTCTATGGAGAGAACTAGAAT
AGTTACTGATGTTTATAAAAAATATGAAGGAATAGTATCAATACCAGTATTAAGAGCATTAGTTTTAAAGGAATTAATGG
AAAGAAAAGAGCTTTGTATTTATGATGGAGAACTTATAGTAGGTGAAAGAGGGGAAGCTGCAGCAGCTACACCAACTTAT
CCAGAATTATGCTGTCATACAGTTGAAGACTTTGATATAATGGATAAACGTGAAAAAATATCCTTTAAAACAACTGATGA
AGATAAAGAAATACAAGAAGAATTAATTATTCCTTTCTGGGAAAAAAGATCTATGAGACATAAAATATTAGAAAAAATGA
CTCCAGAATGGAAAGCCTGTTATGAGGCAGGTATATTCACCGAATTTATGGAACAAAGGGGGCCAGGGCATACTGCAGGT
GGAGATAAATATTATAAAATGGGATTTTTAGATATTAAAGAACAAATAAAAGAAGCTATAAGTAAATTAGATTATTTAAA
TGATGATGAAGCATTAGATAAAAAAGAACAATTAGATGCTATGGATATAGCTTGTGATGCTATAATGATTTATGGAAAAC
GTTATTCAGAATATGCGGCCAAATTAGCACAAAAAGAAGCAGATCCAGTAAGAAAAAAGGAACTTATGGAAATATCTGAA
GTTTGTAGTTGGGTACCAGCTCATGCTCCAAGAACATTTAGAGAAGCAATTCAAATGTACTGGTTTGTTCATTTATGTGT
AATTTCTGAATTAAATCCTTGGGATGCATTTAATCCTGGTAGATTAGATCAACATTTATATCCGTTTTATAAAAAACAAA
TAGAAGAGGGAACATTAGATAGGGAGCAGGCTAGAGAATTGTTGCAATGTTTCTGGGTTAAATTTAATAACCAACCAGCG
CCACCAAAAGTTGGTATAACTTTAAAAGAAAGTGGAACATATACTGACTTTGCAAACATAAATAGTGGTGGTATGAAAGC
TGATGGATCAGATGGTGTTAATGATGTAAGCTATTTAGTACTTGAAGTAATAGATGAAATGAAGTTATTGCAACCAAGTT
CTAATGTACAGATAAGTAAAAAAACCCCTCAAAGATTTTTAAAGAAGGCTTGCGAAGTTATAAGAAAAGGATGGGGCCAA
CCATCAATATTTAATGCTGACTCAGTAGTTCAAGAATTAGTAAGAGCAGGAAAATCTATAGAAGATGCTAGATGCGGAGG
AACTAGTGGTTGTGTTGAAGCTGGAGCTTTTGGTAAAGAAGCTTATATATTAACTGGATACTTTAATTTACCAAAAATAT
TAGAAATAACATTATTAAATGGTGTGGATTCTCAAACTGGTAAACAATTAGGTATAAAAACAGGAGATATAAGTACATTA
AAAACTTATGAAGATTTACTAGATGCATTTAAAAAACAACTTAAATATTTTATTGATATAAAAGTAAATGGTAACAGAGT
TATAGAAAGACTATATGCAACATTGATGCCTGCACCATTCTTATCAGTTGTTACAGATGATTGTATAGCAAAAGGAAAAG
ATTATAATGCAGGGGGAGCAAGATATAATACTAGTTACATTCAAGGTGTTGGTATAGGGACAATCACAGACAGTTTATCA
GCAATCAAATATCAAGTATTTGATGAAAAAAATATAACAATGGAAGAATTAATGGAAGCATTAAGATCTAATTTTGAAGG
ACATGAAGATATATATAACTTAGTTAAGAATAAAACTCCTAAATATGGTAATGATGATGATTATGCAGATGAAATAATGA
AAGAAGTATTTGATGCTTATTATAATGAAGTAAACGGAAGACCTAATGGTAGGGGTGGATGCTATAGAATAGATATGCTA
CCAACAACATGTCATGTTTACTTTGGATCAGTTATAAATGCTACTCCAGATGGAAGAAAAGCTCACATTCCAGTATCAGA
AGGTATTTCTCCATCAAAGGGCGCAGACGTAAATGGACCAACAGGTGTTATAAAATCAGCAGCTAAAATGGATCATTTAA
GAACTGGTGGTACTTTATTGAATCAAAAATTTGTTCCTTCAGTTGTTCAAGGTGAAGAAGGAATAGATAATATGGCTAAT
TTAGTAAGAGCTTATTTTACAATGGACGGGCATCACATTCAATTTAATATTGTAAGTAAGGAAACATTATTAAAAGCTCA
ACAAAATCCGGATGAATATAAAGATTTAATAGTTCGTGTTGCTGGATATAGCGACTACTTTAATAACTTAGATAAAGTTT
TACAAAATGAGATAATAGAAAGAACAGAACAAGAGTTTAATTAA

Upstream 100 bases:

>100_bases
ATTAACTGGTGAGGAAAAACCATCGGATGAGAAAATGAATGAGATAGCAGAAAAATTTAAGCAGGCTGGAATTAAAGTAA
AAATAGGGGGGTAATTAATT

Downstream 100 bases:

>100_bases
AAATTAGTTTAATATATTATTGTAAAGAAAAATAAAAGCTAGGAGAAGGAACTAAGATTTATAATTTAAGTCTTTCTCCT
AGTAACAAATTTTTATTAGG

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 787; Mature: 787

Protein sequence:

>787_residues
MMTDRVKKLREESLKAVPRISMERTRIVTDVYKKYEGIVSIPVLRALVLKELMERKELCIYDGELIVGERGEAAAATPTY
PELCCHTVEDFDIMDKREKISFKTTDEDKEIQEELIIPFWEKRSMRHKILEKMTPEWKACYEAGIFTEFMEQRGPGHTAG
GDKYYKMGFLDIKEQIKEAISKLDYLNDDEALDKKEQLDAMDIACDAIMIYGKRYSEYAAKLAQKEADPVRKKELMEISE
VCSWVPAHAPRTFREAIQMYWFVHLCVISELNPWDAFNPGRLDQHLYPFYKKQIEEGTLDREQARELLQCFWVKFNNQPA
PPKVGITLKESGTYTDFANINSGGMKADGSDGVNDVSYLVLEVIDEMKLLQPSSNVQISKKTPQRFLKKACEVIRKGWGQ
PSIFNADSVVQELVRAGKSIEDARCGGTSGCVEAGAFGKEAYILTGYFNLPKILEITLLNGVDSQTGKQLGIKTGDISTL
KTYEDLLDAFKKQLKYFIDIKVNGNRVIERLYATLMPAPFLSVVTDDCIAKGKDYNAGGARYNTSYIQGVGIGTITDSLS
AIKYQVFDEKNITMEELMEALRSNFEGHEDIYNLVKNKTPKYGNDDDYADEIMKEVFDAYYNEVNGRPNGRGGCYRIDML
PTTCHVYFGSVINATPDGRKAHIPVSEGISPSKGADVNGPTGVIKSAAKMDHLRTGGTLLNQKFVPSVVQGEEGIDNMAN
LVRAYFTMDGHHIQFNIVSKETLLKAQQNPDEYKDLIVRVAGYSDYFNNLDKVLQNEIIERTEQEFN

Sequences:

>Translated_787_residues
MMTDRVKKLREESLKAVPRISMERTRIVTDVYKKYEGIVSIPVLRALVLKELMERKELCIYDGELIVGERGEAAAATPTY
PELCCHTVEDFDIMDKREKISFKTTDEDKEIQEELIIPFWEKRSMRHKILEKMTPEWKACYEAGIFTEFMEQRGPGHTAG
GDKYYKMGFLDIKEQIKEAISKLDYLNDDEALDKKEQLDAMDIACDAIMIYGKRYSEYAAKLAQKEADPVRKKELMEISE
VCSWVPAHAPRTFREAIQMYWFVHLCVISELNPWDAFNPGRLDQHLYPFYKKQIEEGTLDREQARELLQCFWVKFNNQPA
PPKVGITLKESGTYTDFANINSGGMKADGSDGVNDVSYLVLEVIDEMKLLQPSSNVQISKKTPQRFLKKACEVIRKGWGQ
PSIFNADSVVQELVRAGKSIEDARCGGTSGCVEAGAFGKEAYILTGYFNLPKILEITLLNGVDSQTGKQLGIKTGDISTL
KTYEDLLDAFKKQLKYFIDIKVNGNRVIERLYATLMPAPFLSVVTDDCIAKGKDYNAGGARYNTSYIQGVGIGTITDSLS
AIKYQVFDEKNITMEELMEALRSNFEGHEDIYNLVKNKTPKYGNDDDYADEIMKEVFDAYYNEVNGRPNGRGGCYRIDML
PTTCHVYFGSVINATPDGRKAHIPVSEGISPSKGADVNGPTGVIKSAAKMDHLRTGGTLLNQKFVPSVVQGEEGIDNMAN
LVRAYFTMDGHHIQFNIVSKETLLKAQQNPDEYKDLIVRVAGYSDYFNNLDKVLQNEIIERTEQEFN
>Mature_787_residues
MMTDRVKKLREESLKAVPRISMERTRIVTDVYKKYEGIVSIPVLRALVLKELMERKELCIYDGELIVGERGEAAAATPTY
PELCCHTVEDFDIMDKREKISFKTTDEDKEIQEELIIPFWEKRSMRHKILEKMTPEWKACYEAGIFTEFMEQRGPGHTAG
GDKYYKMGFLDIKEQIKEAISKLDYLNDDEALDKKEQLDAMDIACDAIMIYGKRYSEYAAKLAQKEADPVRKKELMEISE
VCSWVPAHAPRTFREAIQMYWFVHLCVISELNPWDAFNPGRLDQHLYPFYKKQIEEGTLDREQARELLQCFWVKFNNQPA
PPKVGITLKESGTYTDFANINSGGMKADGSDGVNDVSYLVLEVIDEMKLLQPSSNVQISKKTPQRFLKKACEVIRKGWGQ
PSIFNADSVVQELVRAGKSIEDARCGGTSGCVEAGAFGKEAYILTGYFNLPKILEITLLNGVDSQTGKQLGIKTGDISTL
KTYEDLLDAFKKQLKYFIDIKVNGNRVIERLYATLMPAPFLSVVTDDCIAKGKDYNAGGARYNTSYIQGVGIGTITDSLS
AIKYQVFDEKNITMEELMEALRSNFEGHEDIYNLVKNKTPKYGNDDDYADEIMKEVFDAYYNEVNGRPNGRGGCYRIDML
PTTCHVYFGSVINATPDGRKAHIPVSEGISPSKGADVNGPTGVIKSAAKMDHLRTGGTLLNQKFVPSVVQGEEGIDNMAN
LVRAYFTMDGHHIQFNIVSKETLLKAQQNPDEYKDLIVRVAGYSDYFNNLDKVLQNEIIERTEQEFN

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=801, Percent_Identity=33.832709113608, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1787044, Length=811, Percent_Identity=32.059186189889, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI48994926, Length=699, Percent_Identity=23.175965665236, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI1787131, Length=680, Percent_Identity=22.3529411764706, Blast_Score=125, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 89067; Mature: 89067

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTDRVKKLREESLKAVPRISMERTRIVTDVYKKYEGIVSIPVLRALVLKELMERKELCI
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEE
YDGELIVGERGEAAAATPTYPELCCHTVEDFDIMDKREKISFKTTDEDKEIQEELIIPFW
ECCEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHCCHHHCCCCCCCCHHHHHHHHCCCHH
EKRSMRHKILEKMTPEWKACYEAGIFTEFMEQRGPGHTAGGDKYYKMGFLDIKEQIKEAI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHH
SKLDYLNDDEALDKKEQLDAMDIACDAIMIYGKRYSEYAAKLAQKEADPVRKKELMEISE
HHHCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VCSWVPAHAPRTFREAIQMYWFVHLCVISELNPWDAFNPGRLDQHLYPFYKKQIEEGTLD
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
REQARELLQCFWVKFNNQPAPPKVGITLKESGTYTDFANINSGGMKADGSDGVNDVSYLV
HHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LEVIDEMKLLQPSSNVQISKKTPQRFLKKACEVIRKGWGQPSIFNADSVVQELVRAGKSI
HHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC
EDARCGGTSGCVEAGAFGKEAYILTGYFNLPKILEITLLNGVDSQTGKQLGIKTGDISTL
CHHCCCCCCCHHHCCCCCCCEEEEEECCCCCHHHEEEEECCCCCCCCCCCCCCCCCCHHH
KTYEDLLDAFKKQLKYFIDIKVNGNRVIERLYATLMPAPFLSVVTDDCIAKGKDYNAGGA
HHHHHHHHHHHHHCEEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
RYNTSYIQGVGIGTITDSLSAIKYQVFDEKNITMEELMEALRSNFEGHEDIYNLVKNKTP
EECCHHHCCCCCHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCC
KYGNDDDYADEIMKEVFDAYYNEVNGRPNGRGGCYRIDMLPTTCHVYFGSVINATPDGRK
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCE
AHIPVSEGISPSKGADVNGPTGVIKSAAKMDHLRTGGTLLNQKFVPSVVQGEEGIDNMAN
EECCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCHHHCCCCCHHHHHH
LVRAYFTMDGHHIQFNIVSKETLLKAQQNPDEYKDLIVRVAGYSDYFNNLDKVLQNEIIE
HHHHHHCCCCCEEEEEEECHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RTEQEFN
HHHHHCC
>Mature Secondary Structure
MMTDRVKKLREESLKAVPRISMERTRIVTDVYKKYEGIVSIPVLRALVLKELMERKELCI
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEE
YDGELIVGERGEAAAATPTYPELCCHTVEDFDIMDKREKISFKTTDEDKEIQEELIIPFW
ECCEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHCCHHHCCCCCCCCHHHHHHHHCCCHH
EKRSMRHKILEKMTPEWKACYEAGIFTEFMEQRGPGHTAGGDKYYKMGFLDIKEQIKEAI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHH
SKLDYLNDDEALDKKEQLDAMDIACDAIMIYGKRYSEYAAKLAQKEADPVRKKELMEISE
HHHCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VCSWVPAHAPRTFREAIQMYWFVHLCVISELNPWDAFNPGRLDQHLYPFYKKQIEEGTLD
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
REQARELLQCFWVKFNNQPAPPKVGITLKESGTYTDFANINSGGMKADGSDGVNDVSYLV
HHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LEVIDEMKLLQPSSNVQISKKTPQRFLKKACEVIRKGWGQPSIFNADSVVQELVRAGKSI
HHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCC
EDARCGGTSGCVEAGAFGKEAYILTGYFNLPKILEITLLNGVDSQTGKQLGIKTGDISTL
CHHCCCCCCCHHHCCCCCCCEEEEEECCCCCHHHEEEEECCCCCCCCCCCCCCCCCCHHH
KTYEDLLDAFKKQLKYFIDIKVNGNRVIERLYATLMPAPFLSVVTDDCIAKGKDYNAGGA
HHHHHHHHHHHHHCEEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
RYNTSYIQGVGIGTITDSLSAIKYQVFDEKNITMEELMEALRSNFEGHEDIYNLVKNKTP
EECCHHHCCCCCHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCC
KYGNDDDYADEIMKEVFDAYYNEVNGRPNGRGGCYRIDMLPTTCHVYFGSVINATPDGRK
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCE
AHIPVSEGISPSKGADVNGPTGVIKSAAKMDHLRTGGTLLNQKFVPSVVQGEEGIDNMAN
EECCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCHHHCCCCCHHHHHH
LVRAYFTMDGHHIQFNIVSKETLLKAQQNPDEYKDLIVRVAGYSDYFNNLDKVLQNEIIE
HHHHHHCCCCCEEEEEEECHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RTEQEFN
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]