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The map label for this gene is trpS [H]

Identifier: 170751702

GI number: 170751702

Start: 5642101

End: 5643165

Strand: Reverse

Name: trpS [H]

Synonym: Mrad2831_5332

Alternate gene names: 170751702

Gene position: 5643165-5642101 (Counterclockwise)

Preceding gene: 170751703

Following gene: 170751701

Centisome position: 92.85

GC content: 69.48

Gene sequence:

>1065_bases
ATGGCCGCGTTCACCGAATTGGTCTTCTCGGGCGTCCAGCCCACCGGCAACCTGCACCTCGGCAACTATCTCGGGGCCGT
GAAGCGCTTCGTGGAGATGCAGGATCGCGACGCGCAGTGCCTGTACTGCGTGGTCGACCTCCACGCGATCACCGTCTGGC
AGGATCCCGAAGCGCTGCGCGGCCAGATCCGCGAGGTCACGGCCGCCTTCCTCGCCGCCGGCATCGATCCCAAGCGCTCG
ATCGTGTTCAACCAGAGCCAGGTGCCGCAGCACGCCGAGCTCGCCTGGGTGTTCAACTGCGTCGCCCGCCTCGGCTGGCT
GAACCGCATGACCCAGTTCAAGGAGAAGGCCGGCAAGGACCGGGAGAACGCCTCGATCGGCCTCTACGACTACCCGGTGC
TGATGGCCGCCGACATCCTGGCCTACCGGGCGACGCACGTGCCGGTCGGCGAGGACCAGAAGCAGCACCTGGAGCTGACC
CGGGACATCGCCCAGAAGTTCAACACCGATTTCTCGGGGTCGATCACGGCGCACGGCCACGGCGACGCCTTCTTCCCGAT
CACGGAGCCGCTGATCGGCGGCCCGGCCGCCCGGGTCATGTCGCTGCGCGACGGCACCAAGAAGATGTCGAAGTCGGACC
CGTCCGACAACGCCCGCATCAACCTCACGGACGATGCGGACGCCATCGCCCAGAAGATCCGCCGGGCCAAGACCGATCCC
GAGCCCCTGCCTTCCGAGGTGGCGGGCCTGAAGGAGCGGCCCGAGGCCGACAACCTCGTGGGGATCTACGCGGCGCTGGC
CGGCATCACCCGGGAGGACGTGCTGCGCGATTTCGGGGGAGCGCAGTTCTCGACCTTCAAGCCGGCGCTCGCCGACCTCG
CGGTGACGAGCCTCGCGCCGATCGCCGGGGAGATGCGCCGCCTCACCGCCGATCCTGGCCACATCGACGCCGTGCTGGCC
GACGGCGGCGCCCGGGCCGAGACCATCGCGGCGCAGACCCTCGACGCGGTCAAGGACATCGTCGGCTTCGTCCGGCGCGG
CCCCAGCCTGCAGGCGGTGCGGTGA

Upstream 100 bases:

>100_bases
GCCGTGAGCGCGTCCTTGACCATTCCGGCCTACGGGTGCGAGTAACCCCGCGCCCGGACGGCGCGGAGCCCCGGTCTTCC
CTTGCTCAGGATCGGCGCCG

Downstream 100 bases:

>100_bases
CCGTCCGGGCGCTGGTCTTCGACGTCTTCGGGACGCTGGTCGACTGGCGCTCCGGCGTCGCCCGCGAGGCGGCGCGGCTG
CTCGCGCCCGTCGCGCCGGA

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MAAFTELVFSGVQPTGNLHLGNYLGAVKRFVEMQDRDAQCLYCVVDLHAITVWQDPEALRGQIREVTAAFLAAGIDPKRS
IVFNQSQVPQHAELAWVFNCVARLGWLNRMTQFKEKAGKDRENASIGLYDYPVLMAADILAYRATHVPVGEDQKQHLELT
RDIAQKFNTDFSGSITAHGHGDAFFPITEPLIGGPAARVMSLRDGTKKMSKSDPSDNARINLTDDADAIAQKIRRAKTDP
EPLPSEVAGLKERPEADNLVGIYAALAGITREDVLRDFGGAQFSTFKPALADLAVTSLAPIAGEMRRLTADPGHIDAVLA
DGGARAETIAAQTLDAVKDIVGFVRRGPSLQAVR

Sequences:

>Translated_354_residues
MAAFTELVFSGVQPTGNLHLGNYLGAVKRFVEMQDRDAQCLYCVVDLHAITVWQDPEALRGQIREVTAAFLAAGIDPKRS
IVFNQSQVPQHAELAWVFNCVARLGWLNRMTQFKEKAGKDRENASIGLYDYPVLMAADILAYRATHVPVGEDQKQHLELT
RDIAQKFNTDFSGSITAHGHGDAFFPITEPLIGGPAARVMSLRDGTKKMSKSDPSDNARINLTDDADAIAQKIRRAKTDP
EPLPSEVAGLKERPEADNLVGIYAALAGITREDVLRDFGGAQFSTFKPALADLAVTSLAPIAGEMRRLTADPGHIDAVLA
DGGARAETIAAQTLDAVKDIVGFVRRGPSLQAVR
>Mature_353_residues
AAFTELVFSGVQPTGNLHLGNYLGAVKRFVEMQDRDAQCLYCVVDLHAITVWQDPEALRGQIREVTAAFLAAGIDPKRSI
VFNQSQVPQHAELAWVFNCVARLGWLNRMTQFKEKAGKDRENASIGLYDYPVLMAADILAYRATHVPVGEDQKQHLELTR
DIAQKFNTDFSGSITAHGHGDAFFPITEPLIGGPAARVMSLRDGTKKMSKSDPSDNARINLTDDADAIAQKIRRAKTDPE
PLPSEVAGLKERPEADNLVGIYAALAGITREDVLRDFGGAQFSTFKPALADLAVTSLAPIAGEMRRLTADPGHIDAVLAD
GGARAETIAAQTLDAVKDIVGFVRRGPSLQAVR

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=341, Percent_Identity=44.8680351906158, Blast_Score=297, Evalue=9e-81,
Organism=Homo sapiens, GI41352700, Length=186, Percent_Identity=50.5376344086022, Blast_Score=206, Evalue=4e-53,
Organism=Escherichia coli, GI1789786, Length=340, Percent_Identity=44.7058823529412, Blast_Score=294, Evalue=7e-81,
Organism=Caenorhabditis elegans, GI71982800, Length=344, Percent_Identity=35.4651162790698, Blast_Score=207, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI71982793, Length=344, Percent_Identity=35.4651162790698, Blast_Score=207, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6320474, Length=361, Percent_Identity=37.3961218836565, Blast_Score=227, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24666151, Length=337, Percent_Identity=39.1691394658754, Blast_Score=234, Evalue=5e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 38416; Mature: 38285

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAFTELVFSGVQPTGNLHLGNYLGAVKRFVEMQDRDAQCLYCVVDLHAITVWQDPEALR
CCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEECCHHHHH
GQIREVTAAFLAAGIDPKRSIVFNQSQVPQHAELAWVFNCVARLGWLNRMTQFKEKAGKD
HHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RENASIGLYDYPVLMAADILAYRATHVPVGEDQKQHLELTRDIAQKFNTDFSGSITAHGH
CCCCEECEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCC
GDAFFPITEPLIGGPAARVMSLRDGTKKMSKSDPSDNARINLTDDADAIAQKIRRAKTDP
CCEECCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCC
EPLPSEVAGLKERPEADNLVGIYAALAGITREDVLRDFGGAQFSTFKPALADLAVTSLAP
CCCCHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IAGEMRRLTADPGHIDAVLADGGARAETIAAQTLDAVKDIVGFVRRGPSLQAVR
HHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AAFTELVFSGVQPTGNLHLGNYLGAVKRFVEMQDRDAQCLYCVVDLHAITVWQDPEALR
CHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEECCHHHHH
GQIREVTAAFLAAGIDPKRSIVFNQSQVPQHAELAWVFNCVARLGWLNRMTQFKEKAGKD
HHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RENASIGLYDYPVLMAADILAYRATHVPVGEDQKQHLELTRDIAQKFNTDFSGSITAHGH
CCCCEECEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCC
GDAFFPITEPLIGGPAARVMSLRDGTKKMSKSDPSDNARINLTDDADAIAQKIRRAKTDP
CCEECCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCC
EPLPSEVAGLKERPEADNLVGIYAALAGITREDVLRDFGGAQFSTFKPALADLAVTSLAP
CCCCHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IAGEMRRLTADPGHIDAVLADGGARAETIAAQTLDAVKDIVGFVRRGPSLQAVR
HHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]