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The map label for this gene is btuF [H]

Identifier: 170751645

GI number: 170751645

Start: 5590909

End: 5591733

Strand: Reverse

Name: btuF [H]

Synonym: Mrad2831_5271

Alternate gene names: 170751645

Gene position: 5591733-5590909 (Counterclockwise)

Preceding gene: 170751648

Following gene: 170751644

Centisome position: 92.0

GC content: 68.48

Gene sequence:

>825_bases
ATGCGTCGCTTCCCGCCTGAACGGATAGTCTGCCTGACCGAGGAGACGGTCGAGACCCTGTACCTGCTGGGAGAGCAGGA
CCGGATCGTCGGCGTGTCCGGCTACGCCGTCCGCCCGCCCCAGGTCCGTCGCGAGAAGCCCCGAGTGTCGGCCTTCACTA
GCGCCGACATCCCCAAGATCCTGGCACTCGCGCCGGACTTGGTCCTGGCCTTCTCGGACATGCAGGCTGGCATCGTCGCC
GACCTCGCCCGGGTCGGGGTCGCGGTCCACCTCTTCAATCAGCGGGACGTGGCCGGCATTCTCGCCATGATCCGGACGGT
GGGCGCCCTCGTGGACGCAAGGGACCGCGCGGAGGCGCTGGCCCGCGGCTACGAGAAACGGCTGTCGACGGTCGCCGAAC
GGGCACAGGGGCGCCCACGGCCCCGGGTGTATTTCGAGGAGTGGGACGAGCCGCTGATCTCAGGCATCGGGTGGGTATCC
GAGCTCGTGCGCATCGCGGGCGGTGATGACGTCTTCCCCGAACTCGCCCGGCAGCCGGCCGCCAAGGACCGCATCGTCAC
GCCGGAGGCGGTGTTGGCCGCCCGGCCGGAAGTCATCCTGGCATCGTGGTGCGGGAAGAAGGTGGTGGCATCCCGCATCG
CGTCGCGGCCCGGCTGGTCGTCCATGCCGGCGGTCGCGGAGGGACGGATCGTCGAGATAAAATCGCCGCTCATCCTCCAG
CCTGGGCCAGCTGCACTCACCGATGGGCTCGACGCCATTCTGCGTGCCTTTGCCGCCCCGATAAATCGTCCGGCTGCGCC
GATCTCAGTTCACAGTCATGCGTAA

Upstream 100 bases:

>100_bases
GAACCAGCGGCTGGATAGTTGGCCAGCGTCGTATTCGTACGTCTGCCATGCCTGGGCCTCCCGCGGACATCGGGCGGTCG
CTTGCGCTATAGGGTATTGT

Downstream 100 bases:

>100_bases
GGAATGGGCCATGACATGCTGATGTCATGGCCACCGCGTTCAGGGCCACTCGCCTTCCACGCGGATTTCGCCATCCAAGC
GAACATTGGAATCCGCAGGA

Product: periplasmic binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MRRFPPERIVCLTEETVETLYLLGEQDRIVGVSGYAVRPPQVRREKPRVSAFTSADIPKILALAPDLVLAFSDMQAGIVA
DLARVGVAVHLFNQRDVAGILAMIRTVGALVDARDRAEALARGYEKRLSTVAERAQGRPRPRVYFEEWDEPLISGIGWVS
ELVRIAGGDDVFPELARQPAAKDRIVTPEAVLAARPEVILASWCGKKVVASRIASRPGWSSMPAVAEGRIVEIKSPLILQ
PGPAALTDGLDAILRAFAAPINRPAAPISVHSHA

Sequences:

>Translated_274_residues
MRRFPPERIVCLTEETVETLYLLGEQDRIVGVSGYAVRPPQVRREKPRVSAFTSADIPKILALAPDLVLAFSDMQAGIVA
DLARVGVAVHLFNQRDVAGILAMIRTVGALVDARDRAEALARGYEKRLSTVAERAQGRPRPRVYFEEWDEPLISGIGWVS
ELVRIAGGDDVFPELARQPAAKDRIVTPEAVLAARPEVILASWCGKKVVASRIASRPGWSSMPAVAEGRIVEIKSPLILQ
PGPAALTDGLDAILRAFAAPINRPAAPISVHSHA
>Mature_274_residues
MRRFPPERIVCLTEETVETLYLLGEQDRIVGVSGYAVRPPQVRREKPRVSAFTSADIPKILALAPDLVLAFSDMQAGIVA
DLARVGVAVHLFNQRDVAGILAMIRTVGALVDARDRAEALARGYEKRLSTVAERAQGRPRPRVYFEEWDEPLISGIGWVS
ELVRIAGGDDVFPELARQPAAKDRIVTPEAVLAARPEVILASWCGKKVVASRIASRPGWSSMPAVAEGRIVEIKSPLILQ
PGPAALTDGLDAILRAFAAPINRPAAPISVHSHA

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=196, Percent_Identity=26.530612244898, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 29683; Mature: 29683

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRFPPERIVCLTEETVETLYLLGEQDRIVGVSGYAVRPPQVRREKPRVSAFTSADIPKI
CCCCCCCCEEEECHHHHHHHHHCCCCCCEEEECCCEECCCHHHHCCCCCHHCCCCCHHHH
LALAPDLVLAFSDMQAGIVADLARVGVAVHLFNQRDVAGILAMIRTVGALVDARDRAEAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHH
ARGYEKRLSTVAERAQGRPRPRVYFEEWDEPLISGIGWVSELVRIAGGDDVFPELARQPA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCC
AKDRIVTPEAVLAARPEVILASWCGKKVVASRIASRPGWSSMPAVAEGRIVEIKSPLILQ
CCCCCCCCHHHHHCCCCCHHHHHCCHHHHHHHHHCCCCCCCCCHHHCCEEEEECCCEEEC
PGPAALTDGLDAILRAFAAPINRPAAPISVHSHA
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRRFPPERIVCLTEETVETLYLLGEQDRIVGVSGYAVRPPQVRREKPRVSAFTSADIPKI
CCCCCCCCEEEECHHHHHHHHHCCCCCCEEEECCCEECCCHHHHCCCCCHHCCCCCHHHH
LALAPDLVLAFSDMQAGIVADLARVGVAVHLFNQRDVAGILAMIRTVGALVDARDRAEAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHH
ARGYEKRLSTVAERAQGRPRPRVYFEEWDEPLISGIGWVSELVRIAGGDDVFPELARQPA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCC
AKDRIVTPEAVLAARPEVILASWCGKKVVASRIASRPGWSSMPAVAEGRIVEIKSPLILQ
CCCCCCCCHHHHHCCCCCHHHHHCCHHHHHHHHHCCCCCCCCCHHHCCEEEEECCCEEEC
PGPAALTDGLDAILRAFAAPINRPAAPISVHSHA
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA