Click here to switch to the map view.

The map label for this gene is mchE [H]

Identifier: 170751256

GI number: 170751256

Start: 5204934

End: 5206319

Strand: Direct

Name: mchE [H]

Synonym: Mrad2831_4873

Alternate gene names: 170751256

Gene position: 5204934-5206319 (Clockwise)

Preceding gene: 170751253

Following gene: 170751257

Centisome position: 85.64

GC content: 72.94

Gene sequence:

>1386_bases
ATGGCCGCGCATGCTGAGCGCATAGTCCTCGAATACGGCTGTCTCTTTCAGGAAGGTTGCTTTCTCCGGGTGCCAAGTCC
GCTCTTTCGACTTGAGGTTATCGAGGCGCGACGCAACGCGTGGCTGGGCGAGGCGCAGATCATCCAGCCTCTGCCGGTCC
GCATCGTGGCCCTGGCCTGCGTGGTCATGGTGGCGGCGTTCGGCCTCTATGCCTGGCAGGGCACCTACACGCGCCGCATC
CACGCCGTCGGTCTGCTCGCGCCCGATATCGGGCTGATCAGCCTCGCCAGCCCGGTGGCGGGGCGCGTCGGCTCGACCGG
CGTCCGCGAGGGCGACAAGGTCGCCCGCGGGCAGCTCCTGTACACGATCGACCTCGATGCCGTGTCGGCGAGCGGCCCCA
CGCAGGAGCGCGTGATCGCCGAGCTGGAGCGGCAGCGCCAGAGCGTGGAGCGGCAGCGGGCCGCCCGGGCCGATCTCGCC
GGCACGGAGCGGCAGGCGCTGCGCGAGCAGGTGGCGAATCTGAGCGAGCAGTCGGCCCAGCTCGCCAAGCAGATCGACCT
GCAGGACAAGCTGGTTCCGCCGCTCCGGGCGCGGGTCGAGGAACTCGCCGACGCCGTGGCGCGCGGCTACGCCCGCGCCG
CCGACTTCCAGAGCCAGAACTACCTCTACATGCAGGCGAGCGCGCAGCTCGCCCAGTTCCAGCAGAGCCGCCTCCAGATC
GACGGCAAGCTCGCCGACACGCGATCCCAGCTCGCCGCCTACGACGACCGGCTGGCCCGCGACCTCGCCGACCTCGACCG
GACCGCCGCCCAGCTGGCGCAGCAGAAGGCCGAGAGCGAGGCGCGCCGCGCCATCGAGGTCCGGGCGCCGGAGGCCGGGA
CCCTGACGTCGATCCGCGCGCAGGTCGGCCAGACCGTGGCGGCCGGGGCGACGCTGCTGACCCTGCTGCCGAGCGCCGGG
CGCCTCCAGGCCAACCTGTTCGTGGATTCGTCGGCCATCGGCTTCGCCGAGGCCGGCGCGCCGGTGATGCTGCGCTACGC
CGCCTTCCCGTTCCAGCGCTTCGGCCTGCACCGCGGCGTCGTCACCGAGGTGACGCGGGCGCCGCTGGGAAGCGACGACG
CGCCCGATTTCGCGCGGCGCGCGGCCGCCCGGGGCGAGGGGACGGACCTCTACCGCATCGTGGTCCGGCCCGATTCCGAC
ACCGTGCTGGCCTACGGCGAGCGGCGCAGCCTCGAGGCGGGCATGCGGGTCGAGGCCGACATCGCCCTGGAGAAGCGGTC
GCTGTACCGCTGGCTGCTCGATCCCGTGGCGCATGTCCGGGAGAGCCTCGCCCTCGTCACGCGCGGCGGCCTCGACGCCC
TGCCGGTCCGGAAGGCCGCGCCGTGA

Upstream 100 bases:

>100_bases
GAGATTTATGGATGACCGCGACGTTCTGACGCACCAGAAAGTATTCAAAATTCGTTGTGGCAACAAGTGTTGACACACAG
ATCACGTGATGCGACCGAGT

Downstream 100 bases:

>100_bases
CGGCGCCGTCCCGCGCCGCCCGGCTGGATTTCGGGCTCCGCCGCCGGGTGCCGGTCGTCCTCCAGGCCGAGGCCGCCGAG
TGCGGCATGGCCTGCCTCGC

Product: secretion protein HlyD family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MAAHAERIVLEYGCLFQEGCFLRVPSPLFRLEVIEARRNAWLGEAQIIQPLPVRIVALACVVMVAAFGLYAWQGTYTRRI
HAVGLLAPDIGLISLASPVAGRVGSTGVREGDKVARGQLLYTIDLDAVSASGPTQERVIAELERQRQSVERQRAARADLA
GTERQALREQVANLSEQSAQLAKQIDLQDKLVPPLRARVEELADAVARGYARAADFQSQNYLYMQASAQLAQFQQSRLQI
DGKLADTRSQLAAYDDRLARDLADLDRTAAQLAQQKAESEARRAIEVRAPEAGTLTSIRAQVGQTVAAGATLLTLLPSAG
RLQANLFVDSSAIGFAEAGAPVMLRYAAFPFQRFGLHRGVVTEVTRAPLGSDDAPDFARRAAARGEGTDLYRIVVRPDSD
TVLAYGERRSLEAGMRVEADIALEKRSLYRWLLDPVAHVRESLALVTRGGLDALPVRKAAP

Sequences:

>Translated_461_residues
MAAHAERIVLEYGCLFQEGCFLRVPSPLFRLEVIEARRNAWLGEAQIIQPLPVRIVALACVVMVAAFGLYAWQGTYTRRI
HAVGLLAPDIGLISLASPVAGRVGSTGVREGDKVARGQLLYTIDLDAVSASGPTQERVIAELERQRQSVERQRAARADLA
GTERQALREQVANLSEQSAQLAKQIDLQDKLVPPLRARVEELADAVARGYARAADFQSQNYLYMQASAQLAQFQQSRLQI
DGKLADTRSQLAAYDDRLARDLADLDRTAAQLAQQKAESEARRAIEVRAPEAGTLTSIRAQVGQTVAAGATLLTLLPSAG
RLQANLFVDSSAIGFAEAGAPVMLRYAAFPFQRFGLHRGVVTEVTRAPLGSDDAPDFARRAAARGEGTDLYRIVVRPDSD
TVLAYGERRSLEAGMRVEADIALEKRSLYRWLLDPVAHVRESLALVTRGGLDALPVRKAAP
>Mature_460_residues
AAHAERIVLEYGCLFQEGCFLRVPSPLFRLEVIEARRNAWLGEAQIIQPLPVRIVALACVVMVAAFGLYAWQGTYTRRIH
AVGLLAPDIGLISLASPVAGRVGSTGVREGDKVARGQLLYTIDLDAVSASGPTQERVIAELERQRQSVERQRAARADLAG
TERQALREQVANLSEQSAQLAKQIDLQDKLVPPLRARVEELADAVARGYARAADFQSQNYLYMQASAQLAQFQQSRLQID
GKLADTRSQLAAYDDRLARDLADLDRTAAQLAQQKAESEARRAIEVRAPEAGTLTSIRAQVGQTVAAGATLLTLLPSAGR
LQANLFVDSSAIGFAEAGAPVMLRYAAFPFQRFGLHRGVVTEVTRAPLGSDDAPDFARRAAARGEGTDLYRIVVRPDSDT
VLAYGERRSLEAGMRVEADIALEKRSLYRWLLDPVAHVRESLALVTRGGLDALPVRKAAP

Specific function: Probably involved, in conjunction with mchF, in the secretion of microcin H47 [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003997
- InterPro:   IPR006143
- InterPro:   IPR006144
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 50184; Mature: 50053

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00543 HLYD_FAMILY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAHAERIVLEYGCLFQEGCFLRVPSPLFRLEVIEARRNAWLGEAQIIQPLPVRIVALAC
CCCCHHHHHHHHCCHHHCCCEEECCCHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHH
VVMVAAFGLYAWQGTYTRRIHAVGLLAPDIGLISLASPVAGRVGSTGVREGDKVARGQLL
HHHHHHHHHHHCCCCHHHHEEEEEEECCCCCHHHHCCHHHCCCCCCCCCCCCHHHCCEEE
YTIDLDAVSASGPTQERVIAELERQRQSVERQRAARADLAGTERQALREQVANLSEQSAQ
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LAKQIDLQDKLVPPLRARVEELADAVARGYARAADFQSQNYLYMQASAQLAQFQQSRLQI
HHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHC
DGKLADTRSQLAAYDDRLARDLADLDRTAAQLAQQKAESEARRAIEVRAPEAGTLTSIRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHH
QVGQTVAAGATLLTLLPSAGRLQANLFVDSSAIGFAEAGAPVMLRYAAFPFQRFGLHRGV
HHCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCEEEHHHHCCHHHHCCCCCH
VTEVTRAPLGSDDAPDFARRAAARGEGTDLYRIVVRPDSDTVLAYGERRSLEAGMRVEAD
HHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCEEEECCCCCCCCCCEEEHH
IALEKRSLYRWLLDPVAHVRESLALVTRGGLDALPVRKAAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AAHAERIVLEYGCLFQEGCFLRVPSPLFRLEVIEARRNAWLGEAQIIQPLPVRIVALAC
CCCHHHHHHHHCCHHHCCCEEECCCHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHH
VVMVAAFGLYAWQGTYTRRIHAVGLLAPDIGLISLASPVAGRVGSTGVREGDKVARGQLL
HHHHHHHHHHHCCCCHHHHEEEEEEECCCCCHHHHCCHHHCCCCCCCCCCCCHHHCCEEE
YTIDLDAVSASGPTQERVIAELERQRQSVERQRAARADLAGTERQALREQVANLSEQSAQ
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LAKQIDLQDKLVPPLRARVEELADAVARGYARAADFQSQNYLYMQASAQLAQFQQSRLQI
HHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHC
DGKLADTRSQLAAYDDRLARDLADLDRTAAQLAQQKAESEARRAIEVRAPEAGTLTSIRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHH
QVGQTVAAGATLLTLLPSAGRLQANLFVDSSAIGFAEAGAPVMLRYAAFPFQRFGLHRGV
HHCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCEEEHHHHCCHHHHCCCCCH
VTEVTRAPLGSDDAPDFARRAAARGEGTDLYRIVVRPDSDTVLAYGERRSLEAGMRVEAD
HHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCEEEECCCCCCCCCCEEEHH
IALEKRSLYRWLLDPVAHVRESLALVTRGGLDALPVRKAAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11181394 [H]