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The map label for this gene is hmp [H]

Identifier: 170751074

GI number: 170751074

Start: 4990472

End: 4992535

Strand: Reverse

Name: hmp [H]

Synonym: Mrad2831_4685

Alternate gene names: 170751074

Gene position: 4992535-4990472 (Counterclockwise)

Preceding gene: 170751075

Following gene: 170751073

Centisome position: 82.14

GC content: 72.34

Gene sequence:

>2064_bases
ATGAGCGACGGTACGACGATGACGAAGCTCTCGACCTGGCACCCGGGCGAGACGGCCATCCAGCAGCAGGTCGGCGTCGC
CGAACGCATGGAGGACGTGGGTCGCCGCGTGGTGCGGGACTACATGCCCGACCAGCATCGCGACTTCTACGAGCAGATCC
CGTTCATCGTGGCCGGCAGCGTCGACGGCCGGGGCGACGCCTGGGCGACCCTGGTCGCCGGCGGTCCCGGGTTCATCGCC
TCCCCGACGCCGACGACCCTCGACATCGCCGTGCGGCTCGATCCGAGCGACCCGGCGAGTCAGGGCATGCGCGCCGGCGA
CGCCATCGGGCTGCTCGGGATCGAGCTTCACACCCGCCGGCGCAACCGCGTGAACGGCCTCATCCGCGCTGCGGCGGACG
GCATCCTGCACGTCGCCGTCGACCAGAGCTTCGGCAACTGCCCGCAGTACATCCAGCTGCGCGATGTCGCCTTCGTCCGC
GACCCGGGCAAGCCCTTCGCGGGCGCCGTCGAGGAGAGCGCCGTGCTCGACGCCGACGCGCGCGCCATGATCGAGGCCGC
CGACACCTTCTTCGTCGCCTCCTACGCCGAGCGCGACGACCGGCGCCAGGTCGACGTCTCCCACCGGGGCGGCAAGGCCG
GCTTCGTGCGGGTCGCCGCCGACGGCACGCTCACGGTCCCCGACTTCGCCGGCAACCTCTTCTTCGCGACGCTCGGCAAC
ATCCTCCTGAACGGCAAGGCCGGCCTGGTCTTCGCAGACTTCGAGACCGGCGACCTCCTGCAGATGACCGGCGACGCCGA
GGTCATCCTGTCCTCGCCCGAGATCGCGGCCTTCCAGGGTGCCGAGCGGCTCTGGACCTTCAAGCCGCGCCGCGTCGTGC
GGCGGCGGGGCGCGCTGCCCCTGCGCTGGACCTTCCGGAAGGACGGCTGGTCGCCGAACTCCCTGATGACCGGCGACTGG
CGCGAGGCCGGCGACCGGCTCCGTGCCTCCGACCTCGCGACGCGCTGGCGCCCGCTCACGGTCACCAAGATCGTGGACGA
GAGCCGCACGATCCGCTCCTTCCACCTCCAGCCGGCCGATGGTGCCGGGCTGATCCCGCATCAGGCCGGGCAGCACCTGC
CCATCCGCGTGACGCTTCCCGGCGCGGACAAACCGATCATCCGCACCTACACCCTCTCGGTTGCGCCCTCGGACGCGGTG
TACCGCATCAGCGTCAAGCGCGACGGGATGGTGTCACGCCACCTGCACGACACGCTTCGGGTCGGCGGCGTGGTCGAGGC
GCGGGCGCCGGCCGGCAGCTTCGTCATCGACGCCCACGCCAGGCGGCCGGCCGTGCTGCTCGCCGGCGGCATCGGCATCA
CACCCCTCCTCGCGATGCTGCGCCACGCCGTCTACGAGGGCCTGCGCACCCGGGGCATCCGGCCGACCACCCTGGTCCAG
GCGGCCCGCTCCAAGGACGAGCGTCCCTTCGACCGGGAGGTGGCGGAGCTTGCGGACGCGGCCCAGGGCGCGGTCCGAGT
GGTGCGGGTGCTGAGCGCGCCCGGGGATGCCGTGGAGGGCGTCGACTACGACGTGGCCGGCAGGATCGACATGGCGCTGC
TCGCCCGCGTGCTGCCGTTCGGCGATTACGACTTCTACCTCTGCGGTCCGGCCGCCTTCACGCAGGCACTCTACGACGGG
CTGCGGGGCCTGAACGTCGCCGACGACCGTATCCACGCCGAGACCTTCGGTCCCTCGTCCCTGGTCCGGAGCGTGCCGGC
GGATGCCGGTGCGGCCGCGCTGCCGCCCGCTTCGACGGAGCCGGTTCCGGTCGCCTTCCTGAACTCGTCGAAGGAGGCGC
GCTGGACGCCGGACGCCGGAACGCTGCTCGAACTCGCCGAGGCCCGCGGCCTCAGCCCGGAGTTCAGTTGCCGGACCGGA
ACCTGCGGCACCTGCCGGACGAAGCTCCTGAAAGGTGCGGTCACCTACGCGAAGGCGCCCACCGGGCCCCACGCGGCCGA
CGAGGTGCTGATCTGCTCGGCCACCCCGGCCGCGGGGAGCGACCCCGTTCATCTCGCCCTCTGA

Upstream 100 bases:

>100_bases
TCGTTCGTCCAAACGCCGGCAGGCCTCACGGCCGCCGCGTGACCTCGCTGGTCCGGGCGCACCTCTGGGTGCGCCCGACC
GGAATGGATGGGAGAGCACC

Downstream 100 bases:

>100_bases
CCGAAGGCGATGGCCGAGGGTCACGTCCCGCCGGCCTGCCGACCGCCCTCCCGACACGCGTCCACCCCGAAACCCCGAGG
AGAAGCCGATGTCCCGTCCC

Product: pyridoxamine 5'-phosphate oxidase-like FMN-binding protein

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 687; Mature: 686

Protein sequence:

>687_residues
MSDGTTMTKLSTWHPGETAIQQQVGVAERMEDVGRRVVRDYMPDQHRDFYEQIPFIVAGSVDGRGDAWATLVAGGPGFIA
SPTPTTLDIAVRLDPSDPASQGMRAGDAIGLLGIELHTRRRNRVNGLIRAAADGILHVAVDQSFGNCPQYIQLRDVAFVR
DPGKPFAGAVEESAVLDADARAMIEAADTFFVASYAERDDRRQVDVSHRGGKAGFVRVAADGTLTVPDFAGNLFFATLGN
ILLNGKAGLVFADFETGDLLQMTGDAEVILSSPEIAAFQGAERLWTFKPRRVVRRRGALPLRWTFRKDGWSPNSLMTGDW
REAGDRLRASDLATRWRPLTVTKIVDESRTIRSFHLQPADGAGLIPHQAGQHLPIRVTLPGADKPIIRTYTLSVAPSDAV
YRISVKRDGMVSRHLHDTLRVGGVVEARAPAGSFVIDAHARRPAVLLAGGIGITPLLAMLRHAVYEGLRTRGIRPTTLVQ
AARSKDERPFDREVAELADAAQGAVRVVRVLSAPGDAVEGVDYDVAGRIDMALLARVLPFGDYDFYLCGPAAFTQALYDG
LRGLNVADDRIHAETFGPSSLVRSVPADAGAAALPPASTEPVPVAFLNSSKEARWTPDAGTLLELAEARGLSPEFSCRTG
TCGTCRTKLLKGAVTYAKAPTGPHAADEVLICSATPAAGSDPVHLAL

Sequences:

>Translated_687_residues
MSDGTTMTKLSTWHPGETAIQQQVGVAERMEDVGRRVVRDYMPDQHRDFYEQIPFIVAGSVDGRGDAWATLVAGGPGFIA
SPTPTTLDIAVRLDPSDPASQGMRAGDAIGLLGIELHTRRRNRVNGLIRAAADGILHVAVDQSFGNCPQYIQLRDVAFVR
DPGKPFAGAVEESAVLDADARAMIEAADTFFVASYAERDDRRQVDVSHRGGKAGFVRVAADGTLTVPDFAGNLFFATLGN
ILLNGKAGLVFADFETGDLLQMTGDAEVILSSPEIAAFQGAERLWTFKPRRVVRRRGALPLRWTFRKDGWSPNSLMTGDW
REAGDRLRASDLATRWRPLTVTKIVDESRTIRSFHLQPADGAGLIPHQAGQHLPIRVTLPGADKPIIRTYTLSVAPSDAV
YRISVKRDGMVSRHLHDTLRVGGVVEARAPAGSFVIDAHARRPAVLLAGGIGITPLLAMLRHAVYEGLRTRGIRPTTLVQ
AARSKDERPFDREVAELADAAQGAVRVVRVLSAPGDAVEGVDYDVAGRIDMALLARVLPFGDYDFYLCGPAAFTQALYDG
LRGLNVADDRIHAETFGPSSLVRSVPADAGAAALPPASTEPVPVAFLNSSKEARWTPDAGTLLELAEARGLSPEFSCRTG
TCGTCRTKLLKGAVTYAKAPTGPHAADEVLICSATPAAGSDPVHLAL
>Mature_686_residues
SDGTTMTKLSTWHPGETAIQQQVGVAERMEDVGRRVVRDYMPDQHRDFYEQIPFIVAGSVDGRGDAWATLVAGGPGFIAS
PTPTTLDIAVRLDPSDPASQGMRAGDAIGLLGIELHTRRRNRVNGLIRAAADGILHVAVDQSFGNCPQYIQLRDVAFVRD
PGKPFAGAVEESAVLDADARAMIEAADTFFVASYAERDDRRQVDVSHRGGKAGFVRVAADGTLTVPDFAGNLFFATLGNI
LLNGKAGLVFADFETGDLLQMTGDAEVILSSPEIAAFQGAERLWTFKPRRVVRRRGALPLRWTFRKDGWSPNSLMTGDWR
EAGDRLRASDLATRWRPLTVTKIVDESRTIRSFHLQPADGAGLIPHQAGQHLPIRVTLPGADKPIIRTYTLSVAPSDAVY
RISVKRDGMVSRHLHDTLRVGGVVEARAPAGSFVIDAHARRPAVLLAGGIGITPLLAMLRHAVYEGLRTRGIRPTTLVQA
ARSKDERPFDREVAELADAAQGAVRVVRVLSAPGDAVEGVDYDVAGRIDMALLARVLPFGDYDFYLCGPAAFTQALYDGL
RGLNVADDRIHAETFGPSSLVRSVPADAGAAALPPASTEPVPVAFLNSSKEARWTPDAGTLLELAEARGLSPEFSCRTGT
CGTCRTKLLKGAVTYAKAPTGPHAADEVLICSATPAAGSDPVHLAL

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG3576

COG function: function code R; Predicted flavin-nucleotide-binding protein structurally related to pyridoxine 5'-phosphate oxidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=238, Percent_Identity=33.1932773109244, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787658, Length=362, Percent_Identity=28.7292817679558, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6321673, Length=265, Percent_Identity=30.188679245283, Blast_Score=103, Evalue=8e-23,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 73675; Mature: 73544

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGTTMTKLSTWHPGETAIQQQVGVAERMEDVGRRVVRDYMPDQHRDFYEQIPFIVAGS
CCCCCEEEEEECCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEEC
VDGRGDAWATLVAGGPGFIASPTPTTLDIAVRLDPSDPASQGMRAGDAIGLLGIELHTRR
CCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCHHHCCCCCCCEEEEEEEEEHHH
RNRVNGLIRAAADGILHVAVDQSFGNCPQYIQLRDVAFVRDPGKPFAGAVEESAVLDADA
HHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCCCHHHEECCCH
RAMIEAADTFFVASYAERDDRRQVDVSHRGGKAGFVRVAADGTLTVPDFAGNLFFATLGN
HHHHHHHHHHEEEHHHCCCCCCEECCCCCCCCCEEEEEECCCEEECCCCCCCHHHHHHHH
ILLNGKAGLVFADFETGDLLQMTGDAEVILSSPEIAAFQGAERLWTFKPRRVVRRRGALP
EEECCCCCEEEEECCCCCEEEECCCCEEEEECCCCHHHCCCHHHEECCHHHHHHHCCCCC
LRWTFRKDGWSPNSLMTGDWREAGDRLRASDLATRWRPLTVTKIVDESRTIRSFHLQPAD
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCC
GAGLIPHQAGQHLPIRVTLPGADKPIIRTYTLSVAPSDAVYRISVKRDGMVSRHLHDTLR
CCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHH
VGGVVEARAPAGSFVIDAHARRPAVLLAGGIGITPLLAMLRHAVYEGLRTRGIRPTTLVQ
HCCEEEECCCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AARSKDERPFDREVAELADAAQGAVRVVRVLSAPGDAVEGVDYDVAGRIDMALLARVLPF
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
GDYDFYLCGPAAFTQALYDGLRGLNVADDRIHAETFGPSSLVRSVPADAGAAALPPASTE
CCCCEEEECHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHCCCCCCCCCCCCCCCC
PVPVAFLNSSKEARWTPDAGTLLELAEARGLSPEFSCRTGTCGTCRTKLLKGAVTYAKAP
CEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
TGPHAADEVLICSATPAAGSDPVHLAL
CCCCCCCCEEEEECCCCCCCCCEEEEC
>Mature Secondary Structure 
SDGTTMTKLSTWHPGETAIQQQVGVAERMEDVGRRVVRDYMPDQHRDFYEQIPFIVAGS
CCCCEEEEEECCCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEEC
VDGRGDAWATLVAGGPGFIASPTPTTLDIAVRLDPSDPASQGMRAGDAIGLLGIELHTRR
CCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCHHHCCCCCCCEEEEEEEEEHHH
RNRVNGLIRAAADGILHVAVDQSFGNCPQYIQLRDVAFVRDPGKPFAGAVEESAVLDADA
HHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCCCHHHEECCCH
RAMIEAADTFFVASYAERDDRRQVDVSHRGGKAGFVRVAADGTLTVPDFAGNLFFATLGN
HHHHHHHHHHEEEHHHCCCCCCEECCCCCCCCCEEEEEECCCEEECCCCCCCHHHHHHHH
ILLNGKAGLVFADFETGDLLQMTGDAEVILSSPEIAAFQGAERLWTFKPRRVVRRRGALP
EEECCCCCEEEEECCCCCEEEECCCCEEEEECCCCHHHCCCHHHEECCHHHHHHHCCCCC
LRWTFRKDGWSPNSLMTGDWREAGDRLRASDLATRWRPLTVTKIVDESRTIRSFHLQPAD
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCC
GAGLIPHQAGQHLPIRVTLPGADKPIIRTYTLSVAPSDAVYRISVKRDGMVSRHLHDTLR
CCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHH
VGGVVEARAPAGSFVIDAHARRPAVLLAGGIGITPLLAMLRHAVYEGLRTRGIRPTTLVQ
HCCEEEECCCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AARSKDERPFDREVAELADAAQGAVRVVRVLSAPGDAVEGVDYDVAGRIDMALLARVLPF
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
GDYDFYLCGPAAFTQALYDGLRGLNVADDRIHAETFGPSSLVRSVPADAGAAALPPASTE
CCCCEEEECHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHCCCCCCCCCCCCCCCC
PVPVAFLNSSKEARWTPDAGTLLELAEARGLSPEFSCRTGTCGTCRTKLLKGAVTYAKAP
CEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
TGPHAADEVLICSATPAAGSDPVHLAL
CCCCCCCCEEEEECCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]