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The map label for this gene is lutR [H]

Identifier: 170751033

GI number: 170751033

Start: 4939741

End: 4940565

Strand: Reverse

Name: lutR [H]

Synonym: Mrad2831_4644

Alternate gene names: 170751033

Gene position: 4940565-4939741 (Counterclockwise)

Preceding gene: 170751037

Following gene: 170751032

Centisome position: 81.29

GC content: 76.85

Gene sequence:

>825_bases
ATGTCGTCAGCGATGTCCCCTTCCCTGTCCGGCTCCCCTGACCCGGCGGAGGCGCCCGGGCAGCGGGCGCTCCGCGGCGC
GGAGCCGGCACGGCCCGCCTCCCTGGCCGACGGCCTCTATCAGGCGATCCGCACCGGCATCGCGAACGGCACCTACGGGC
CCGACGCGCGGCTCCCGAGCGAGCACGACCTCGCGGGACGCTTCCAGGTCTCGCGGCCGGTGGTGCGGGCCGCCCTCGAC
CGGCTGCGCCGGGAGGGCGCGATCGTCTCGCGGCAGGGGGCCGGCAGCTTCGTGCGGGCGCGGCCGGTGCGCCCGGCCCT
GGGCTTCGCCCCCGTGGAGAGCGTCGCCGACATCCAGCGCTGCTACGAGTTCCGCCTCACCCTCGAGCCGGAGGCGGCCT
ACCACGCGGCGCTGCGCCACGACGCGGCGGCCCTCGACCGGATCGCGGCGGCCCTCGATCGGCTGGCGGTCGCGACCCGG
CAGGAGCAGCACCGGGCGGACGCCGATTTCGCCTTCCACCACGCGATCGCGGCGGCGTCGAACAACCACTACTACGCGGC
CTGCCTGGAGGCGCTGCAATCCCACATCGCCGTGGGCATGACGATCCACGGCCTCGCCCTGCAGGGCCCGCGCTCCGGCC
TGCGCGGCGTGCTGGAGGAGCATCGCGGGATCTTCGCGGCGATCGAGGGCCGCGACGGCGCGGGCGCCCGCAGCCGCATG
GCCGCCCATATCGCCGGGTCCCGCGACCGGCTGTTCGAGGGGCGGCTCCTCGATCTCTCGCTCGGACGGCCCGCGGAGGC
CGGCGCCGCCGCGCCGCCCGCTTGA

Upstream 100 bases:

>100_bases
GTCGGCGCAACTGGGGCCCGGATCAGCACAAATGTTGACAAGTCCGCCGGCGCAACGCTGACTCGGCGCGGATCGGCTCG
ATCCACGGAGGCGTCCCCGG

Downstream 100 bases:

>100_bases
CCGCCGCGCCGGCCGGTCTGTATGCCTCGCGACAACCTGACAACAAGGACGCGAGCCGCGCTCGGCCGGGCGGCGCGGCC
GGCGAGGCTCCCGCCATGAT

Product: GntR domain-containing protein

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSSAMSPSLSGSPDPAEAPGQRALRGAEPARPASLADGLYQAIRTGIANGTYGPDARLPSEHDLAGRFQVSRPVVRAALD
RLRREGAIVSRQGAGSFVRARPVRPALGFAPVESVADIQRCYEFRLTLEPEAAYHAALRHDAAALDRIAAALDRLAVATR
QEQHRADADFAFHHAIAAASNNHYYAACLEALQSHIAVGMTIHGLALQGPRSGLRGVLEEHRGIFAAIEGRDGAGARSRM
AAHIAGSRDRLFEGRLLDLSLGRPAEAGAAAPPA

Sequences:

>Translated_274_residues
MSSAMSPSLSGSPDPAEAPGQRALRGAEPARPASLADGLYQAIRTGIANGTYGPDARLPSEHDLAGRFQVSRPVVRAALD
RLRREGAIVSRQGAGSFVRARPVRPALGFAPVESVADIQRCYEFRLTLEPEAAYHAALRHDAAALDRIAAALDRLAVATR
QEQHRADADFAFHHAIAAASNNHYYAACLEALQSHIAVGMTIHGLALQGPRSGLRGVLEEHRGIFAAIEGRDGAGARSRM
AAHIAGSRDRLFEGRLLDLSLGRPAEAGAAAPPA
>Mature_273_residues
SSAMSPSLSGSPDPAEAPGQRALRGAEPARPASLADGLYQAIRTGIANGTYGPDARLPSEHDLAGRFQVSRPVVRAALDR
LRREGAIVSRQGAGSFVRARPVRPALGFAPVESVADIQRCYEFRLTLEPEAAYHAALRHDAAALDRIAAALDRLAVATRQ
EQHRADADFAFHHAIAAASNNHYYAACLEALQSHIAVGMTIHGLALQGPRSGLRGVLEEHRGIFAAIEGRDGAGARSRMA
AHIAGSRDRLFEGRLLDLSLGRPAEAGAAAPPA

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790032, Length=229, Percent_Identity=29.6943231441048, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1786303, Length=250, Percent_Identity=30, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI48994955, Length=215, Percent_Identity=26.5116279069767, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 28935; Mature: 28804

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAMSPSLSGSPDPAEAPGQRALRGAEPARPASLADGLYQAIRTGIANGTYGPDARLPS
CCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
EHDLAGRFQVSRPVVRAALDRLRREGAIVSRQGAGSFVRARPVRPALGFAPVESVADIQR
CCCCCCCEEHHHHHHHHHHHHHHHCCCEEECCCCCCCEECCCCCCCCCCCCHHHHHHHHH
CYEFRLTLEPEAAYHAALRHDAAALDRIAAALDRLAVATRQEQHRADADFAFHHAIAAAS
HHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
NNHYYAACLEALQSHIAVGMTIHGLALQGPRSGLRGVLEEHRGIFAAIEGRDGAGARSRM
CCCHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHH
AAHIAGSRDRLFEGRLLDLSLGRPAEAGAAAPPA
HHHHCCCHHHHHCCEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SSAMSPSLSGSPDPAEAPGQRALRGAEPARPASLADGLYQAIRTGIANGTYGPDARLPS
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
EHDLAGRFQVSRPVVRAALDRLRREGAIVSRQGAGSFVRARPVRPALGFAPVESVADIQR
CCCCCCCEEHHHHHHHHHHHHHHHCCCEEECCCCCCCEECCCCCCCCCCCCHHHHHHHHH
CYEFRLTLEPEAAYHAALRHDAAALDRIAAALDRLAVATRQEQHRADADFAFHHAIAAAS
HHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
NNHYYAACLEALQSHIAVGMTIHGLALQGPRSGLRGVLEEHRGIFAAIEGRDGAGARSRM
CCCHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHH
AAHIAGSRDRLFEGRLLDLSLGRPAEAGAAAPPA
HHHHCCCHHHHHCCEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]