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The map label for this gene is folE [H]

Identifier: 170751028

GI number: 170751028

Start: 4935479

End: 4936297

Strand: Reverse

Name: folE [H]

Synonym: Mrad2831_4639

Alternate gene names: 170751028

Gene position: 4936297-4935479 (Counterclockwise)

Preceding gene: 170751031

Following gene: 170751027

Centisome position: 81.22

GC content: 69.47

Gene sequence:

>819_bases
ATGTACGCCAAGCCCGACAGCACCCCGATGAAATCCCGCATCGCGCGGGCCGGAGATGCCATGGATGCCGCGCTCAAATC
CTTGTCCTACCAGGGTGACGGCCAGGGTTTCGACCGGAACGGGATCGACGGGCAAGGTCTGAACGGCATGCGCAATGACA
ATCGGCCCGCCGATGACGGGCGGCCCGTCGAGGTGGCGCCCGAGCCGACCTCCGCGACCCGCGTCCCGGACGGGATCGAG
ACCGGCCGCCCGAGCCGCGCCGAGGCCGAGGCCGCCGTCCGCACCCTCCTGCGCTGGGCCGGCGACGACCCGACCCGCGA
GGGTCTGCTCGACACCCCGGCCCGGGTCACCAAGGCCTACGAGCAGCTGTTCGGCGGCTACGCGGTCGACGCCGAGAAGT
TGCTGGAGCGCGTCTTCGAGGAGGTCGAGGGCTACTCGGACATCGTGCTGGTGCGCGACATCCCGTTCCACTCCCACTGC
GAGCACCACATGGTGCCGTTCATGGGGCTCGCCCACATCGCCTACTATCCGACCCGGGGCGTGGTCGGCCTGTCGAAGCT
CGCCCGCGTGGTCGACACCTTCGCCCGCCGCCTGCAGACGCAGGAGACCATGACCGCCCAGATCGCCGACGTGATCGAGA
GCATCCTCAAGCCCCGCGGCGTCGCCGTGATGGTGGAGGCCGAGCATCTCTGCATGGCGATGCGCGGCGTCCAGAAGGCC
GGCGTCTCGACCATCACGAGCCAGTTCCGCGGCGTCTTCAAGGACAACGCCAACGAGCAGGTCCGCTTCCTGACCCTGGT
GCGCGGCGGCGCCAAGTAG

Upstream 100 bases:

>100_bases
GGCCACGGTGGTTCCCACGGCCTTTCGTCCATCCCGGGGCGCCGGCTTGAAACCTTTTCGAGCCCGGGATCTTCCCTCGA
ACGCCCGACGCGATCCGACG

Downstream 100 bases:

>100_bases
CTCCGACGAGCGAGCCGGATCACCCTGCCATGACCGCGCGCGACATCGCCTTCGATCCCCCCGGCACCCGCGCCGAGGTC
GAGGAGGGCGCGGCCCTCAC

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MYAKPDSTPMKSRIARAGDAMDAALKSLSYQGDGQGFDRNGIDGQGLNGMRNDNRPADDGRPVEVAPEPTSATRVPDGIE
TGRPSRAEAEAAVRTLLRWAGDDPTREGLLDTPARVTKAYEQLFGGYAVDAEKLLERVFEEVEGYSDIVLVRDIPFHSHC
EHHMVPFMGLAHIAYYPTRGVVGLSKLARVVDTFARRLQTQETMTAQIADVIESILKPRGVAVMVEAEHLCMAMRGVQKA
GVSTITSQFRGVFKDNANEQVRFLTLVRGGAK

Sequences:

>Translated_272_residues
MYAKPDSTPMKSRIARAGDAMDAALKSLSYQGDGQGFDRNGIDGQGLNGMRNDNRPADDGRPVEVAPEPTSATRVPDGIE
TGRPSRAEAEAAVRTLLRWAGDDPTREGLLDTPARVTKAYEQLFGGYAVDAEKLLERVFEEVEGYSDIVLVRDIPFHSHC
EHHMVPFMGLAHIAYYPTRGVVGLSKLARVVDTFARRLQTQETMTAQIADVIESILKPRGVAVMVEAEHLCMAMRGVQKA
GVSTITSQFRGVFKDNANEQVRFLTLVRGGAK
>Mature_272_residues
MYAKPDSTPMKSRIARAGDAMDAALKSLSYQGDGQGFDRNGIDGQGLNGMRNDNRPADDGRPVEVAPEPTSATRVPDGIE
TGRPSRAEAEAAVRTLLRWAGDDPTREGLLDTPARVTKAYEQLFGGYAVDAEKLLERVFEEVEGYSDIVLVRDIPFHSHC
EHHMVPFMGLAHIAYYPTRGVVGLSKLARVVDTFARRLQTQETMTAQIADVIESILKPRGVAVMVEAEHLCMAMRGVQKA
GVSTITSQFRGVFKDNANEQVRFLTLVRGGAK

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=229, Percent_Identity=42.3580786026201, Blast_Score=175, Evalue=4e-44,
Organism=Homo sapiens, GI4503949, Length=229, Percent_Identity=42.3580786026201, Blast_Score=175, Evalue=4e-44,
Organism=Homo sapiens, GI66932970, Length=187, Percent_Identity=41.1764705882353, Blast_Score=130, Evalue=9e-31,
Organism=Homo sapiens, GI66932972, Length=187, Percent_Identity=41.1764705882353, Blast_Score=130, Evalue=1e-30,
Organism=Escherichia coli, GI1788476, Length=176, Percent_Identity=36.9318181818182, Blast_Score=104, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17560486, Length=177, Percent_Identity=48.0225988700565, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6321706, Length=178, Percent_Identity=48.876404494382, Blast_Score=168, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24656782, Length=177, Percent_Identity=46.3276836158192, Blast_Score=148, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24656791, Length=179, Percent_Identity=45.8100558659218, Blast_Score=148, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24656796, Length=179, Percent_Identity=45.8100558659218, Blast_Score=147, Evalue=5e-36,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 29761; Mature: 29761

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYAKPDSTPMKSRIARAGDAMDAALKSLSYQGDGQGFDRNGIDGQGLNGMRNDNRPADDG
CCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RPVEVAPEPTSATRVPDGIETGRPSRAEAEAAVRTLLRWAGDDPTREGLLDTPARVTKAY
CCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
EQLFGGYAVDAEKLLERVFEEVEGYSDIVLVRDIPFHSHCEHHMVPFMGLAHIAYYPTRG
HHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCHHHHHHHHHHHHHHCCCCC
VVGLSKLARVVDTFARRLQTQETMTAQIADVIESILKPRGVAVMVEAEHLCMAMRGVQKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHH
GVSTITSQFRGVFKDNANEQVRFLTLVRGGAK
HHHHHHHHHHHHHHCCCCCCEEEHEEECCCCC
>Mature Secondary Structure
MYAKPDSTPMKSRIARAGDAMDAALKSLSYQGDGQGFDRNGIDGQGLNGMRNDNRPADDG
CCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RPVEVAPEPTSATRVPDGIETGRPSRAEAEAAVRTLLRWAGDDPTREGLLDTPARVTKAY
CCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
EQLFGGYAVDAEKLLERVFEEVEGYSDIVLVRDIPFHSHCEHHMVPFMGLAHIAYYPTRG
HHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCHHHHHHHHHHHHHHCCCCC
VVGLSKLARVVDTFARRLQTQETMTAQIADVIESILKPRGVAVMVEAEHLCMAMRGVQKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHH
GVSTITSQFRGVFKDNANEQVRFLTLVRGGAK
HHHHHHHHHHHHHHCCCCCCEEEHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA