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The map label for this gene is trmL [H]

Identifier: 170751018

GI number: 170751018

Start: 4927069

End: 4927599

Strand: Reverse

Name: trmL [H]

Synonym: Mrad2831_4629

Alternate gene names: 170751018

Gene position: 4927599-4927069 (Counterclockwise)

Preceding gene: 170751021

Following gene: 170751017

Centisome position: 81.07

GC content: 75.52

Gene sequence:

>531_bases
ATGCCGCGGCGGTTTGCCGGCTGCGCGTCGGCGCGGCGCGCCTTTGACGGCCGGGCCCGCGCCCCGCACAAGGCCGGCGC
CATGCTCCGCCTCGCCCTCTACCAGCCCGACATTCCCCAGAACACCGGCACGATGCTGCGCATGGCCGCCTGCCTCGGGC
TGGCGGTGGAGATCGTGGAGCCCGCGGGCTTCGACGTCTCGGACCGCCACCTGCGGCGCTCGGGCCTCGACTACCTCGAC
CACGTGGCGATCACCCGGCACCGCTCGTTCGCGGCCTTCGCGGCGTGGCGGGCCGAGGCCGGGATCCGGCTCGTCCTCGC
CACCACGGCGGGCGCCGTACCCTACACGGACTTCGCCTTCCGCCCGGACGATTGCCTGATGGTCGGGCGCGAATCCGCCG
GCGTGCCGGAGGCGGTGCACGCGGCGGCCGACGCGCGGATCGTGGTGCCGATCCGGCCGGGCCTGCGCTCGCTCAACGTC
GCGGTCGCGGCCGCGATGATCGCCGGCGAGGCCCTGCGTCAGGTGGGCTGA

Upstream 100 bases:

>100_bases
CGCATCGGAGCAGGTCTTTCAGAGAGGCCGAACACGGGGTGGCGCCGCCTCAAGGTTCTGGCGGGGCTTAAGTTCCGCGC
GGTCTCGGCGCCGGCCGGGG

Downstream 100 bases:

>100_bases
CCGCCGCCGAAGACAGCGCGCACGATGACGGCTTCCCGCCGTTTTCGCGAGCGGACGGAAGCGATCCAGGGCCGCGCGCC
CTCCGTGACCGGGGCGCCGG

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MPRRFAGCASARRAFDGRARAPHKAGAMLRLALYQPDIPQNTGTMLRMAACLGLAVEIVEPAGFDVSDRHLRRSGLDYLD
HVAITRHRSFAAFAAWRAEAGIRLVLATTAGAVPYTDFAFRPDDCLMVGRESAGVPEAVHAAADARIVVPIRPGLRSLNV
AVAAAMIAGEALRQVG

Sequences:

>Translated_176_residues
MPRRFAGCASARRAFDGRARAPHKAGAMLRLALYQPDIPQNTGTMLRMAACLGLAVEIVEPAGFDVSDRHLRRSGLDYLD
HVAITRHRSFAAFAAWRAEAGIRLVLATTAGAVPYTDFAFRPDDCLMVGRESAGVPEAVHAAADARIVVPIRPGLRSLNV
AVAAAMIAGEALRQVG
>Mature_175_residues
PRRFAGCASARRAFDGRARAPHKAGAMLRLALYQPDIPQNTGTMLRMAACLGLAVEIVEPAGFDVSDRHLRRSGLDYLDH
VAITRHRSFAAFAAWRAEAGIRLVLATTAGAVPYTDFAFRPDDCLMVGRESAGVPEAVHAAADARIVVPIRPGLRSLNVA
VAAAMIAGEALRQVG

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=151, Percent_Identity=39.7350993377483, Blast_Score=116, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18788; Mature: 18657

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRFAGCASARRAFDGRARAPHKAGAMLRLALYQPDIPQNTGTMLRMAACLGLAVEIVE
CCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEC
PAGFDVSDRHLRRSGLDYLDHVAITRHRSFAAFAAWRAEAGIRLVLATTAGAVPYTDFAF
CCCCCCCHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCC
RPDDCLMVGRESAGVPEAVHAAADARIVVPIRPGLRSLNVAVAAAMIAGEALRQVG
CCCCEEEECCCCCCCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PRRFAGCASARRAFDGRARAPHKAGAMLRLALYQPDIPQNTGTMLRMAACLGLAVEIVE
CCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEC
PAGFDVSDRHLRRSGLDYLDHVAITRHRSFAAFAAWRAEAGIRLVLATTAGAVPYTDFAF
CCCCCCCHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCC
RPDDCLMVGRESAGVPEAVHAAADARIVVPIRPGLRSLNVAVAAAMIAGEALRQVG
CCCCEEEECCCCCCCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA