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The map label for this gene is appB [H]

Identifier: 170750969

GI number: 170750969

Start: 4876103

End: 4877062

Strand: Direct

Name: appB [H]

Synonym: Mrad2831_4580

Alternate gene names: 170750969

Gene position: 4876103-4877062 (Clockwise)

Preceding gene: 170750968

Following gene: 170750970

Centisome position: 80.23

GC content: 69.17

Gene sequence:

>960_bases
ATGGGCGCCTACATCCTGCGACGCCTCCTGATCGCGATCCCGAGCCTGCTCGGCATCAGCCTGATCCTGTTCACCGTCCT
GGCGCTCGCGCCGGGCGACCCGTTCGGCGAGCTCGCCAGCAACCCGAACGTGCCCCCGGAGGTGCGGGCGGCCCTGCGGG
TGAAGTTCGGCCTCGATGACCCGATCCTCACCCGCTACCTGCACTGGCTCACCGCCATGCTGCAGGGCGACTGGGGCTTC
TCGTTCGCGAGCCGGGTGAACGTCGACACGCTGATCCTGCAGCGGCTGCCGACGACCCTGTTCGTGGTCGGCTCCTCCCA
GGTCCTGGCGCTGCTGATCGCGATCCCGGTGGGCCTCACCGCGGCGCGCCGGCCCTACTCGCTCGCCGACCAGGTCGCCA
ACACGCTGGCCTTCGTGGGCTTCTCGCTGCCGACCTTCTTCACCGGCCTGCTGTTCATCCTGCTGTTCTCGATCAAGCTC
GGCTGGCTGCCCTTCGTCTACCAGGCCGACCTGCCGGGCTCCGGCCTGCCCTGGATCTGGGCGAATGTCCGGCAGGCGAT
CATGCCGGTGGCGGTGCTGGGATTCTTCCAGGCCGCCTCCTACACCCGCTACGTGCGCGCCTCGGCGCTGGACGTCGCCC
GCCTCGACTACGTCACCACGGCCCGGGCCAAGGGCCTGACCGAGGGCACGGTGACCCGCCGGCACATCGCCCGCAACGCC
CTGATCCCGGTGGTGACGCTCGTCGCCCTCCAGATCCCGGCGGTGTTCGGTGGCGCCATCGTCACCGAGCAGATCTTCCG
GATTCCCGGCATCGGCTCGCTGCTGATCGACGCCATGCTGGCCAACGACACGCCGGTGGTGATGGCCGTGACCTTCGTGT
TCGCCGGCCTCGTCATCCTGTTCAACCTCGTCGCGGACCTGCTCTATGGCTGGCTCGACCCTCGCATCGCCCTCCGATGA

Upstream 100 bases:

>100_bases
CGGTGGGCGCCACGCTCACGGCGCCCCGCAGCGGCTGGGACAACGACCTGTCGTTCCTGCCCGACTGGTACAGGGAGGCC
TGAGCGGGAGCCTGAGCGCC

Downstream 100 bases:

>100_bases
GGCGGTGCCCGTCGCCCGCGCGCCGGCTTCGCCGGGGCGGGAGACGTGGCGGCGCTTCCGGCGGCACCGGCTGGCGCTCG
CCAGCGTCGGCGTGCTGGCG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MGAYILRRLLIAIPSLLGISLILFTVLALAPGDPFGELASNPNVPPEVRAALRVKFGLDDPILTRYLHWLTAMLQGDWGF
SFASRVNVDTLILQRLPTTLFVVGSSQVLALLIAIPVGLTAARRPYSLADQVANTLAFVGFSLPTFFTGLLFILLFSIKL
GWLPFVYQADLPGSGLPWIWANVRQAIMPVAVLGFFQAASYTRYVRASALDVARLDYVTTARAKGLTEGTVTRRHIARNA
LIPVVTLVALQIPAVFGGAIVTEQIFRIPGIGSLLIDAMLANDTPVVMAVTFVFAGLVILFNLVADLLYGWLDPRIALR

Sequences:

>Translated_319_residues
MGAYILRRLLIAIPSLLGISLILFTVLALAPGDPFGELASNPNVPPEVRAALRVKFGLDDPILTRYLHWLTAMLQGDWGF
SFASRVNVDTLILQRLPTTLFVVGSSQVLALLIAIPVGLTAARRPYSLADQVANTLAFVGFSLPTFFTGLLFILLFSIKL
GWLPFVYQADLPGSGLPWIWANVRQAIMPVAVLGFFQAASYTRYVRASALDVARLDYVTTARAKGLTEGTVTRRHIARNA
LIPVVTLVALQIPAVFGGAIVTEQIFRIPGIGSLLIDAMLANDTPVVMAVTFVFAGLVILFNLVADLLYGWLDPRIALR
>Mature_318_residues
GAYILRRLLIAIPSLLGISLILFTVLALAPGDPFGELASNPNVPPEVRAALRVKFGLDDPILTRYLHWLTAMLQGDWGFS
FASRVNVDTLILQRLPTTLFVVGSSQVLALLIAIPVGLTAARRPYSLADQVANTLAFVGFSLPTFFTGLLFILLFSIKLG
WLPFVYQADLPGSGLPWIWANVRQAIMPVAVLGFFQAASYTRYVRASALDVARLDYVTTARAKGLTEGTVTRRHIARNAL
IPVVTLVALQIPAVFGGAIVTEQIFRIPGIGSLLIDAMLANDTPVVMAVTFVFAGLVILFNLVADLLYGWLDPRIALR

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=335, Percent_Identity=38.5074626865672, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1787053, Length=319, Percent_Identity=38.2445141065831, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1789888, Length=315, Percent_Identity=38.7301587301587, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1787497, Length=317, Percent_Identity=33.4384858044164, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1788504, Length=364, Percent_Identity=29.9450549450549, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1787550, Length=268, Percent_Identity=30.5970149253731, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787761, Length=318, Percent_Identity=31.1320754716981, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34659; Mature: 34527

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAYILRRLLIAIPSLLGISLILFTVLALAPGDPFGELASNPNVPPEVRAALRVKFGLDD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHEECCCCC
PILTRYLHWLTAMLQGDWGFSFASRVNVDTLILQRLPTTLFVVGSSQVLALLIAIPVGLT
HHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCH
AARRPYSLADQVANTLAFVGFSLPTFFTGLLFILLFSIKLGWLPFVYQADLPGSGLPWIW
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHH
ANVRQAIMPVAVLGFFQAASYTRYVRASALDVARLDYVTTARAKGLTEGTVTRRHIARNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LIPVVTLVALQIPAVFGGAIVTEQIFRIPGIGSLLIDAMLANDTPVVMAVTFVFAGLVIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FNLVADLLYGWLDPRIALR
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GAYILRRLLIAIPSLLGISLILFTVLALAPGDPFGELASNPNVPPEVRAALRVKFGLDD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHEECCCCC
PILTRYLHWLTAMLQGDWGFSFASRVNVDTLILQRLPTTLFVVGSSQVLALLIAIPVGLT
HHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCH
AARRPYSLADQVANTLAFVGFSLPTFFTGLLFILLFSIKLGWLPFVYQADLPGSGLPWIW
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHH
ANVRQAIMPVAVLGFFQAASYTRYVRASALDVARLDYVTTARAKGLTEGTVTRRHIARNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LIPVVTLVALQIPAVFGGAIVTEQIFRIPGIGSLLIDAMLANDTPVVMAVTFVFAGLVIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FNLVADLLYGWLDPRIALR
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]