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The map label for this gene is appA [H]

Identifier: 170750968

GI number: 170750968

Start: 4874295

End: 4876085

Strand: Direct

Name: appA [H]

Synonym: Mrad2831_4579

Alternate gene names: 170750968

Gene position: 4874295-4876085 (Clockwise)

Preceding gene: 170750966

Following gene: 170750969

Centisome position: 80.2

GC content: 68.84

Gene sequence:

>1791_bases
ATGGACGAGCGGGATCTGCGCGGCCTCATCGGGCGGGTGAAGGGCGGTGGCCTGTCGCGGCGCGCCTTCGTGCAGCGGAT
GGTGGCCCTGGGCCTCACCGCGCCCATGGCCGGGCTGATGCTGGCCGGGAACGGTGTCGCGATGGCGGCCGATATCCGGT
CCGGCTACAAGCCGACCAAGGCCGGCGGCGGCGGCGCGCTCAAGCTGCTCTGGTGGCAGGCGCCGACGCTGATCAACCCG
CATTTCGCCATCGGCACCAAGGACCAGGACGCCTCGCGGATCTTCTACGAGCCGCTCGCCGCCTGGGACGCGGACGGCAA
CCTCGTGCCGGTTCTGGCCGCCGCGATCCCGTCCAAGGAGAACGGGGCGCTCGCCGCCGACGGCCGCTCGGTGGTCTGGA
CGCTCAAGCCCGGCGTGAAGTGGCACGACGGCAAGCCGCTCACCGCCGACGACCTCGTCTTCACCTGGGAGTACGCCCGC
GACCCCGCCACCGCGGCGGTGACGGCCGGGTCCTACAAGGATTGCAAGGTCGAGAAGGTCGACGACCTCAGCGTCCGGGT
GCTGTTCGACAAGCCGACGCCCTACTGGTGCGACGCCTTCGTGGGCATCGTCGGGATGGTGCTGCCGAAGCACCTGTTCG
GCCCGTACAGCGGCGCCAAGTCCCGGGACGCGCCGCAGAACCTCGCCCCCGTGGGCACCGGCCCGTACCGGTTTGTGGAG
TTCCGGCCCGGCGACATCGTCCGCGGGGAGCGCAACCCGGATTACCACCTGCCGAACCGGCCGTATTTCGACACGATCGA
GATGAAGGGCGGCGGGGACGCGGTCTCGGCCGCCCGCGCGGTGCTCCAGACCGGCGAGTACGACTACGCCTGGAACATGC
TGATCGAGGACGAGGTGCTCAAGCGCCTGGAGACCGGCGGCAAGGGCCGGGTCGACGTGGTCTACGGCGGCAAGCTCGAG
TTCCTGCTCCTCAACGCCACCGACCCGAACGTCGAGGTCGACGGCGAGCGCGCCTCGATCACGACGAAGCACCCCGCCTT
CTCCGACCCGAAGGTGTGCCAGGCGATGAACCTGCTGGTCGACCGCAAGTCGATCCAGACCTACATCTACGGCCGCACCG
GCAAGCCCACCGCCAACACGGTCAACGGCCCGGAGCGCTTCGTCTCCAAGAACACGAGCTTCGCCTTCGACCCGGCCAAG
GCCAACGCGCTCCTCGACGAGGCCGGCTGGAAGAAGGGCTCCGACGGCATCCGCGCCAAGGACGGCAAGAAGCTGAAGCT
CGTCTTCCAGACCTCGATCAACGCGCCGCGCCAGAAGACCCAGGCGATCATCAAGCAGGCGGCCGCCAAGGCCGGCATCG
AGATCGAGCTGAAATCGGTGACCGGCTCGGTGTTCTTCTCCTCCGACCCGGCGAACCCCGACACCTGCACGCATTTCTAC
GCCGACATGGAGATGTACGCCTACAGCATGACGCAGGCCGATCCGGCGATCTGGCTGCTGATGTACGCCTCCTGGGAGGT
GGCCCAGAAGGCCAACAAGTGGCAGGGCCGCAACGTCGTGCGCTGGCGCAACGACGCCTACGACAAGGCCTACAACGCCG
CCCAGAGCGAGCTCGACCCGGTCAAGCGCGCGGCGCTCCTGATCACCTGCAACGACCTCGCGGTGTCCGAGAACGTGCTG
CCGCTGATCCACCGCGCCGAGGTCTCGGCGGTGGGCGCCACGCTCACGGCGCCCCGCAGCGGCTGGGACAACGACCTGTC
GTTCCTGCCCGACTGGTACAGGGAGGCCTGA

Upstream 100 bases:

>100_bases
CCCTCGACACGTTTCGGTTGAAGCGTCACTGGCCCGCCCCTTGCCAAGGCCGCCACCGGAATGTTGCTGTGCCGCTCGCG
GCGGTAAACGGGATGCGTAC

Downstream 100 bases:

>100_bases
GCGGGAGCCTGAGCGCCATGGGCGCCTACATCCTGCGACGCCTCCTGATCGCGATCCCGAGCCTGCTCGGCATCAGCCTG
ATCCTGTTCACCGTCCTGGC

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MDERDLRGLIGRVKGGGLSRRAFVQRMVALGLTAPMAGLMLAGNGVAMAADIRSGYKPTKAGGGGALKLLWWQAPTLINP
HFAIGTKDQDASRIFYEPLAAWDADGNLVPVLAAAIPSKENGALAADGRSVVWTLKPGVKWHDGKPLTADDLVFTWEYAR
DPATAAVTAGSYKDCKVEKVDDLSVRVLFDKPTPYWCDAFVGIVGMVLPKHLFGPYSGAKSRDAPQNLAPVGTGPYRFVE
FRPGDIVRGERNPDYHLPNRPYFDTIEMKGGGDAVSAARAVLQTGEYDYAWNMLIEDEVLKRLETGGKGRVDVVYGGKLE
FLLLNATDPNVEVDGERASITTKHPAFSDPKVCQAMNLLVDRKSIQTYIYGRTGKPTANTVNGPERFVSKNTSFAFDPAK
ANALLDEAGWKKGSDGIRAKDGKKLKLVFQTSINAPRQKTQAIIKQAAAKAGIEIELKSVTGSVFFSSDPANPDTCTHFY
ADMEMYAYSMTQADPAIWLLMYASWEVAQKANKWQGRNVVRWRNDAYDKAYNAAQSELDPVKRAALLITCNDLAVSENVL
PLIHRAEVSAVGATLTAPRSGWDNDLSFLPDWYREA

Sequences:

>Translated_596_residues
MDERDLRGLIGRVKGGGLSRRAFVQRMVALGLTAPMAGLMLAGNGVAMAADIRSGYKPTKAGGGGALKLLWWQAPTLINP
HFAIGTKDQDASRIFYEPLAAWDADGNLVPVLAAAIPSKENGALAADGRSVVWTLKPGVKWHDGKPLTADDLVFTWEYAR
DPATAAVTAGSYKDCKVEKVDDLSVRVLFDKPTPYWCDAFVGIVGMVLPKHLFGPYSGAKSRDAPQNLAPVGTGPYRFVE
FRPGDIVRGERNPDYHLPNRPYFDTIEMKGGGDAVSAARAVLQTGEYDYAWNMLIEDEVLKRLETGGKGRVDVVYGGKLE
FLLLNATDPNVEVDGERASITTKHPAFSDPKVCQAMNLLVDRKSIQTYIYGRTGKPTANTVNGPERFVSKNTSFAFDPAK
ANALLDEAGWKKGSDGIRAKDGKKLKLVFQTSINAPRQKTQAIIKQAAAKAGIEIELKSVTGSVFFSSDPANPDTCTHFY
ADMEMYAYSMTQADPAIWLLMYASWEVAQKANKWQGRNVVRWRNDAYDKAYNAAQSELDPVKRAALLITCNDLAVSENVL
PLIHRAEVSAVGATLTAPRSGWDNDLSFLPDWYREA
>Mature_596_residues
MDERDLRGLIGRVKGGGLSRRAFVQRMVALGLTAPMAGLMLAGNGVAMAADIRSGYKPTKAGGGGALKLLWWQAPTLINP
HFAIGTKDQDASRIFYEPLAAWDADGNLVPVLAAAIPSKENGALAADGRSVVWTLKPGVKWHDGKPLTADDLVFTWEYAR
DPATAAVTAGSYKDCKVEKVDDLSVRVLFDKPTPYWCDAFVGIVGMVLPKHLFGPYSGAKSRDAPQNLAPVGTGPYRFVE
FRPGDIVRGERNPDYHLPNRPYFDTIEMKGGGDAVSAARAVLQTGEYDYAWNMLIEDEVLKRLETGGKGRVDVVYGGKLE
FLLLNATDPNVEVDGERASITTKHPAFSDPKVCQAMNLLVDRKSIQTYIYGRTGKPTANTVNGPERFVSKNTSFAFDPAK
ANALLDEAGWKKGSDGIRAKDGKKLKLVFQTSINAPRQKTQAIIKQAAAKAGIEIELKSVTGSVFFSSDPANPDTCTHFY
ADMEMYAYSMTQADPAIWLLMYASWEVAQKANKWQGRNVVRWRNDAYDKAYNAAQSELDPVKRAALLITCNDLAVSENVL
PLIHRAEVSAVGATLTAPRSGWDNDLSFLPDWYREA

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=452, Percent_Identity=23.8938053097345, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789397, Length=479, Percent_Identity=21.294363256785, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1787495, Length=376, Percent_Identity=24.468085106383, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 65119; Mature: 65119

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDERDLRGLIGRVKGGGLSRRAFVQRMVALGLTAPMAGLMLAGNGVAMAADIRSGYKPTK
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHCEEEECCCEEEEEHHHCCCCCCC
AGGGGALKLLWWQAPTLINPHFAIGTKDQDASRIFYEPLAAWDADGNLVPVLAAAIPSKE
CCCCCEEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHCCCCC
NGALAADGRSVVWTLKPGVKWHDGKPLTADDLVFTWEYARDPATAAVTAGSYKDCKVEKV
CCEEEECCCEEEEEECCCCEECCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCEEE
DDLSVRVLFDKPTPYWCDAFVGIVGMVLPKHLFGPYSGAKSRDAPQNLAPVGTGPYRFVE
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEE
FRPGDIVRGERNPDYHLPNRPYFDTIEMKGGGDAVSAARAVLQTGEYDYAWNMLIEDEVL
ECCCCEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEHHHHH
KRLETGGKGRVDVVYGGKLEFLLLNATDPNVEVDGERASITTKHPAFSDPKVCQAMNLLV
HHHHCCCCCEEEEEECCEEEEEEEECCCCCEEECCCCEEEEECCCCCCCHHHHHHHHHHH
DRKSIQTYIYGRTGKPTANTVNGPERFVSKNTSFAFDPAKANALLDEAGWKKGSDGIRAK
HHCCEEEEEEECCCCCCCCCCCCHHHHHCCCCCEEECCHHHHHHHHHCCCCCCCCCCCCC
DGKKLKLVFQTSINAPRQKTQAIIKQAAAKAGIEIELKSVTGSVFFSSDPANPDTCTHFY
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCCCHHHHHH
ADMEMYAYSMTQADPAIWLLMYASWEVAQKANKWQGRNVVRWRNDAYDKAYNAAQSELDP
HHHHHEEEECCCCCCEEEEEEECCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHCH
VKRAALLITCNDLAVSENVLPLIHRAEVSAVGATLTAPRSGWDNDLSFLPDWYREA
HHHEEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure
MDERDLRGLIGRVKGGGLSRRAFVQRMVALGLTAPMAGLMLAGNGVAMAADIRSGYKPTK
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHCEEEECCCEEEEEHHHCCCCCCC
AGGGGALKLLWWQAPTLINPHFAIGTKDQDASRIFYEPLAAWDADGNLVPVLAAAIPSKE
CCCCCEEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHCCCCC
NGALAADGRSVVWTLKPGVKWHDGKPLTADDLVFTWEYARDPATAAVTAGSYKDCKVEKV
CCEEEECCCEEEEEECCCCEECCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCEEE
DDLSVRVLFDKPTPYWCDAFVGIVGMVLPKHLFGPYSGAKSRDAPQNLAPVGTGPYRFVE
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEE
FRPGDIVRGERNPDYHLPNRPYFDTIEMKGGGDAVSAARAVLQTGEYDYAWNMLIEDEVL
ECCCCEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEHHHHH
KRLETGGKGRVDVVYGGKLEFLLLNATDPNVEVDGERASITTKHPAFSDPKVCQAMNLLV
HHHHCCCCCEEEEEECCEEEEEEEECCCCCEEECCCCEEEEECCCCCCCHHHHHHHHHHH
DRKSIQTYIYGRTGKPTANTVNGPERFVSKNTSFAFDPAKANALLDEAGWKKGSDGIRAK
HHCCEEEEEEECCCCCCCCCCCCHHHHHCCCCCEEECCHHHHHHHHHCCCCCCCCCCCCC
DGKKLKLVFQTSINAPRQKTQAIIKQAAAKAGIEIELKSVTGSVFFSSDPANPDTCTHFY
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCCCHHHHHH
ADMEMYAYSMTQADPAIWLLMYASWEVAQKANKWQGRNVVRWRNDAYDKAYNAAQSELDP
HHHHHEEEECCCCCCEEEEEEECCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHCH
VKRAALLITCNDLAVSENVLPLIHRAEVSAVGATLTAPRSGWDNDLSFLPDWYREA
HHHEEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]