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The map label for this gene is dhfrIII [H]

Identifier: 170750915

GI number: 170750915

Start: 4804029

End: 4804529

Strand: Direct

Name: dhfrIII [H]

Synonym: Mrad2831_4526

Alternate gene names: 170750915

Gene position: 4804029-4804529 (Clockwise)

Preceding gene: 170750914

Following gene: 170750916

Centisome position: 79.04

GC content: 74.05

Gene sequence:

>501_bases
ATGAGCGCGACCGTCGCCCCGCTCGTCTCCCTGGTCGCCGCCGTCGCCCGCAACGGCGTGATCGGCCGCGACAACGGGCT
CGCCTGGCATCTCTCCAGCGACCTCAAGCGCTTCAAGGCCCTGACCATGGGCAAGCCGATGCTGATGGGCCGGCGCACCT
GGGATTCGATCGGCCGGCCCCTGCCGGGGCGCCGCACCCTGGTGCTCACCCGGGATACGGGCTTCCGCGCCGAGGGCGTC
GAGACCGTCCACGACTGGGACTCCGCGCTGGCCGCCGCCGGGGCGGAGCTGATGGTGGTCGGCGGTGCCGAGATCTACGC
GCTCGCGCTCCCGCACGCCGACCGGCTCCATCTCACCGAGGTCGAGGCCGAGCCCGCCGGCGACGTGCGATTCCCCGCCT
TCGACCGCGCCCGCTTCCGGGAGACGTTCCGTGAGGCGCACCCCGCGGGCCCGCGGGACGAGCACGCCTTCGCCTTCGTC
GACTGGGAGCGCGCACGCTGA

Upstream 100 bases:

>100_bases
GGCTCAACCCGGAGGTCCGCGACCTCTTCGCCTTCCGGTTCGAGGACATCGCCATCGAGAACTACGACCCGCACCCCGCC
ATCGCGGCCCCGGTGGCGGT

Downstream 100 bases:

>100_bases
GGCGCGCCGCCGCATTTGCGTTCCGCGCCGCGCATGCCCACCTGCCAGCCTTGACAGCACGGGGCGTGCGCCCGCAGGTG
CCTGTCGGGTGCGCGCGCTC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MSATVAPLVSLVAAVARNGVIGRDNGLAWHLSSDLKRFKALTMGKPMLMGRRTWDSIGRPLPGRRTLVLTRDTGFRAEGV
ETVHDWDSALAAAGAELMVVGGAEIYALALPHADRLHLTEVEAEPAGDVRFPAFDRARFRETFREAHPAGPRDEHAFAFV
DWERAR

Sequences:

>Translated_166_residues
MSATVAPLVSLVAAVARNGVIGRDNGLAWHLSSDLKRFKALTMGKPMLMGRRTWDSIGRPLPGRRTLVLTRDTGFRAEGV
ETVHDWDSALAAAGAELMVVGGAEIYALALPHADRLHLTEVEAEPAGDVRFPAFDRARFRETFREAHPAGPRDEHAFAFV
DWERAR
>Mature_165_residues
SATVAPLVSLVAAVARNGVIGRDNGLAWHLSSDLKRFKALTMGKPMLMGRRTWDSIGRPLPGRRTLVLTRDTGFRAEGVE
TVHDWDSALAAAGAELMVVGGAEIYALALPHADRLHLTEVEAEPAGDVRFPAFDRARFRETFREAHPAGPRDEHAFAFVD
WERAR

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=159, Percent_Identity=42.1383647798742, Blast_Score=134, Evalue=3e-33,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18241; Mature: 18110

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATVAPLVSLVAAVARNGVIGRDNGLAWHLSSDLKRFKALTMGKPMLMGRRTWDSIGRP
CCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHCCCCHHCCCCCHHHHCCC
LPGRRTLVLTRDTGFRAEGVETVHDWDSALAAAGAELMVVGGAEIYALALPHADRLHLTE
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEEEECCCCCCEEEEE
VEAEPAGDVRFPAFDRARFRETFREAHPAGPRDEHAFAFVDWERAR
ECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure 
SATVAPLVSLVAAVARNGVIGRDNGLAWHLSSDLKRFKALTMGKPMLMGRRTWDSIGRP
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHCCCCHHCCCCCHHHHCCC
LPGRRTLVLTRDTGFRAEGVETVHDWDSALAAAGAELMVVGGAEIYALALPHADRLHLTE
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEEEECCCCCCEEEEE
VEAEPAGDVRFPAFDRARFRETFREAHPAGPRDEHAFAFVDWERAR
ECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]