Click here to switch to the map view.

The map label for this gene is thyA [H]

Identifier: 170750914

GI number: 170750914

Start: 4803238

End: 4804032

Strand: Direct

Name: thyA [H]

Synonym: Mrad2831_4525

Alternate gene names: 170750914

Gene position: 4803238-4804032 (Clockwise)

Preceding gene: 170750913

Following gene: 170750915

Centisome position: 79.03

GC content: 70.31

Gene sequence:

>795_bases
ATGGACGCATATCACGACCTGCTCCGGCGCATCCTCGACGAGGGCGTCGCCAAGGACGACCGCACCGGGACCGGCACGCT
CTCGGTGTTCGGCCACCAGATGCGCTTCGACCTGTCGGCGGGCTTCCCGCTGGTCACCACGAAGCGGCTGCACCTGCGCT
CGATCATCCACGAGCTGCTCTGGTTCCTGGCCGGCGACACCAACGTCGCGGCGTTGCGGGCGAACGGCGTGACGATCTGG
GACGAGTGGGCCGACGCGAACGGCGACCTCGGCCCGGTCTACGGCCGGCAGTGGCGCTCCTGGGAGAGGCCCGGGGGCGG
CACGGTCGACCAGATCGCCTGGGTGCTCGACGAGATCCGGCGCAACCCCGATTCCCGCCGCCTGATCGTCTCGGCCTGGA
ACCCGGCCGACCTCGACCGGATGGCGCTCGCCCCCTGCCACTGCCTGTTCCAGTTCTACGTCAGCGAGGGGCGGCTCTCC
TGCCAGCTCTACCAGCGCTCGGCGGACGCCTTCCTGGGCGTGCCCTTCAATATCGCGAGCTACGCCCTCCTGACCCACAT
GATCGCCCAGGTGACCGGCCTCGGGGTCGGCGCCTTCGTCCACACCTTCGGCGACGCCCACCTCTACCGGAACCACCTGG
AGCAGACCCGCACCCTGCTGGGCAGGTCCCCGCGCCCGCTGCCGCAGCTGCGGCTCAACCCGGAGGTCCGCGACCTCTTC
GCCTTCCGGTTCGAGGACATCGCCATCGAGAACTACGACCCGCACCCCGCCATCGCGGCCCCGGTGGCGGTATGA

Upstream 100 bases:

>100_bases
CGGCCGGACGCCGCCCGCGGACAGGCTCCCGGCGCGACGGCGGTGGACATATCGCGGCGGCGCGCTTGCCCGCCGGCGCG
CCGGCGCGCAGGAATGGGCG

Downstream 100 bases:

>100_bases
GCGCGACCGTCGCCCCGCTCGTCTCCCTGGTCGCCGCCGTCGCCCGCAACGGCGTGATCGGCCGCGACAACGGGCTCGCC
TGGCATCTCTCCAGCGACCT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MDAYHDLLRRILDEGVAKDDRTGTGTLSVFGHQMRFDLSAGFPLVTTKRLHLRSIIHELLWFLAGDTNVAALRANGVTIW
DEWADANGDLGPVYGRQWRSWERPGGGTVDQIAWVLDEIRRNPDSRRLIVSAWNPADLDRMALAPCHCLFQFYVSEGRLS
CQLYQRSADAFLGVPFNIASYALLTHMIAQVTGLGVGAFVHTFGDAHLYRNHLEQTRTLLGRSPRPLPQLRLNPEVRDLF
AFRFEDIAIENYDPHPAIAAPVAV

Sequences:

>Translated_264_residues
MDAYHDLLRRILDEGVAKDDRTGTGTLSVFGHQMRFDLSAGFPLVTTKRLHLRSIIHELLWFLAGDTNVAALRANGVTIW
DEWADANGDLGPVYGRQWRSWERPGGGTVDQIAWVLDEIRRNPDSRRLIVSAWNPADLDRMALAPCHCLFQFYVSEGRLS
CQLYQRSADAFLGVPFNIASYALLTHMIAQVTGLGVGAFVHTFGDAHLYRNHLEQTRTLLGRSPRPLPQLRLNPEVRDLF
AFRFEDIAIENYDPHPAIAAPVAV
>Mature_264_residues
MDAYHDLLRRILDEGVAKDDRTGTGTLSVFGHQMRFDLSAGFPLVTTKRLHLRSIIHELLWFLAGDTNVAALRANGVTIW
DEWADANGDLGPVYGRQWRSWERPGGGTVDQIAWVLDEIRRNPDSRRLIVSAWNPADLDRMALAPCHCLFQFYVSEGRLS
CQLYQRSADAFLGVPFNIASYALLTHMIAQVTGLGVGAFVHTFGDAHLYRNHLEQTRTLLGRSPRPLPQLRLNPEVRDLF
AFRFEDIAIENYDPHPAIAAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=281, Percent_Identity=52.6690391459075, Blast_Score=285, Evalue=2e-77,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=66.6666666666667, Blast_Score=384, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=46.9964664310954, Blast_Score=263, Evalue=6e-71,
Organism=Saccharomyces cerevisiae, GI6324648, Length=292, Percent_Identity=41.0958904109589, Blast_Score=209, Evalue=4e-55,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=55.8303886925795, Blast_Score=304, Evalue=3e-83,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 29717; Mature: 29717

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAYHDLLRRILDEGVAKDDRTGTGTLSVFGHQMRFDLSAGFPLVTTKRLHLRSIIHELL
CCHHHHHHHHHHHCCCCCCCCCCCCCCEEECCEEEEECCCCCCEEEHHHHHHHHHHHHHH
WFLAGDTNVAALRANGVTIWDEWADANGDLGPVYGRQWRSWERPGGGTVDQIAWVLDEIR
HHHCCCCCEEEEEECCEEEEECCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHH
RNPDSRRLIVSAWNPADLDRMALAPCHCLFQFYVSEGRLSCQLYQRSADAFLGVPFNIAS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCCEECCCHHHHH
YALLTHMIAQVTGLGVGAFVHTFGDAHLYRNHLEQTRTLLGRSPRPLPQLRLNPEVRDLF
HHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
AFRFEDIAIENYDPHPAIAAPVAV
HHHHHHEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MDAYHDLLRRILDEGVAKDDRTGTGTLSVFGHQMRFDLSAGFPLVTTKRLHLRSIIHELL
CCHHHHHHHHHHHCCCCCCCCCCCCCCEEECCEEEEECCCCCCEEEHHHHHHHHHHHHHH
WFLAGDTNVAALRANGVTIWDEWADANGDLGPVYGRQWRSWERPGGGTVDQIAWVLDEIR
HHHCCCCCEEEEEECCEEEEECCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHH
RNPDSRRLIVSAWNPADLDRMALAPCHCLFQFYVSEGRLSCQLYQRSADAFLGVPFNIAS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCCEECCCHHHHH
YALLTHMIAQVTGLGVGAFVHTFGDAHLYRNHLEQTRTLLGRSPRPLPQLRLNPEVRDLF
HHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
AFRFEDIAIENYDPHPAIAAPVAV
HHHHHHEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA