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The map label for this gene is dapE [H]

Identifier: 170750872

GI number: 170750872

Start: 4762929

End: 4764314

Strand: Reverse

Name: dapE [H]

Synonym: Mrad2831_4483

Alternate gene names: 170750872

Gene position: 4764314-4762929 (Counterclockwise)

Preceding gene: 170750878

Following gene: 170750870

Centisome position: 78.39

GC content: 70.85

Gene sequence:

>1386_bases
ATGGCCGCGCTCGACGCGATCCTCAACGACATCGACACGGACTTGGAGAACGCCCTGGAGCGGCTGTTCGCCTTCCTGCG
GATCCCGTCGATCTCCACCGATCCCGCCTATGCCGGCCCGTGCCGCGAGGCCGCCGCCTGGCTCGCCAAGGACCTGACCG
CGCTCGGCTTCGAGACCTCCGTGGAGGAGACCTCGCTCCATCCGGTCGTGCTGGCGCACAAGCCGAAGCCCGGGGCGCCC
CACGTCCTGTTCTACGGCCATTACGACGTGCAGCCCGTCGACCCGCTCGATCTCTGGACGACGCCGCCCTTCGAGCCGCG
CATCGCCGAGGACGGCCGGGGCGGCAAGACGATCGTCGGGCGCGGCGCCTCCGACGACAAGGGTCAGGTGATGACCTTCG
TGGAGGCCTGCCGGGCCACCATCGCCATGACCGGCGAGCTGCCCTGCGGCGTCACGATATTGATCGAGGGCGCCGAGGAG
AGCGGCTCGCAGGGACTTCCCGAGTGGGTCGCGGCCAACCGCGAGAGACTCGGCTGCGACGTGGTCCTCGTCTGCGACAC
CGGCATGTGGGACCGGAAGACCCCGGCCATCACCTCGTCGCTGCGCGGGCTCGCCTATTTCGAGGTGAAGGTCACCTGCG
CCGACCGCGACCTGCATTCGGGCTTCTTCGGCGGCGCCGCCCGCAACCCGATCCACGTGCTGGCCAAGATCATCGCCGAC
CTGCACGACGCGGACGGGCGCGTGACCATCCCGGGCTTCTACGAGGGCGTGCACGAGCGCCCGCCCGAGGTCCTGGAGCA
GTGGCGCGGCCTCGGCCTGACGCCGGAGACGCTGCTCGGACCGATCGGCCTCAAGGAGCCCGCGGGCGAGCGCGGCCGGA
TGCCGATCGAACTGGTGCAGTCGCGCCCCTCCTGCGACGCCAACGGCATCGTCGGCGGCTATACCGGCGAGGGCACCAAG
ACGGTGATCGCCTCCACCGCCTCCGCCAAGGTCTCGTTCCGCCTCGTGGACGACCAGGACCCGAAGCGGCTCGCCGAGAG
CTTCGAGGCCTTCGTGCGCGCGCGGGTGCCGGCGGACTGCCAGGTCGAGGTCATCACCTACAAGGGCTCGCGGGCGATCA
GCCTGCCCTTCGACATGCCGCAGCTCGGGGCCGCCAAGGCGGCGCTGCAGGACGAGTGGGGGGTGCCCGCGGTCACCGTC
GGCGCGGGCGGCTCGATCCCGATCGTCGGCGACTTCAAGCGGATCCTGGGGCGCGACACCCTGCTGATCGGCTTCGGGCT
CGACGACGACCGGATCCACTCGCCCAACGAGAAGTACGACCTGACGAGCTTCCACAAGGGCACGCGCTCCTGGGCGCGGA
TCCTCGCGGCCCTGGGCCAGGGCTGA

Upstream 100 bases:

>100_bases
GTCCGACGTTGCCGCCGTGGCCCCGCCTCGGCTAACCCTCGTGCGCGCGGAATTCCCGCAGAGTTCTCGCAGAGTCCACG
CCGTACCCCGGAGCCCCCCG

Downstream 100 bases:

>100_bases
GCCCGGCGGAGGGCCGATCTCAGCGGGCGGGCGCCGCCCGCATCTTCCCGTCGATCCAGGCCCAGAGCGCCCGGACCTCC
TCGGCGGCCTGGCTGCCGGG

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MAALDAILNDIDTDLENALERLFAFLRIPSISTDPAYAGPCREAAAWLAKDLTALGFETSVEETSLHPVVLAHKPKPGAP
HVLFYGHYDVQPVDPLDLWTTPPFEPRIAEDGRGGKTIVGRGASDDKGQVMTFVEACRATIAMTGELPCGVTILIEGAEE
SGSQGLPEWVAANRERLGCDVVLVCDTGMWDRKTPAITSSLRGLAYFEVKVTCADRDLHSGFFGGAARNPIHVLAKIIAD
LHDADGRVTIPGFYEGVHERPPEVLEQWRGLGLTPETLLGPIGLKEPAGERGRMPIELVQSRPSCDANGIVGGYTGEGTK
TVIASTASAKVSFRLVDDQDPKRLAESFEAFVRARVPADCQVEVITYKGSRAISLPFDMPQLGAAKAALQDEWGVPAVTV
GAGGSIPIVGDFKRILGRDTLLIGFGLDDDRIHSPNEKYDLTSFHKGTRSWARILAALGQG

Sequences:

>Translated_461_residues
MAALDAILNDIDTDLENALERLFAFLRIPSISTDPAYAGPCREAAAWLAKDLTALGFETSVEETSLHPVVLAHKPKPGAP
HVLFYGHYDVQPVDPLDLWTTPPFEPRIAEDGRGGKTIVGRGASDDKGQVMTFVEACRATIAMTGELPCGVTILIEGAEE
SGSQGLPEWVAANRERLGCDVVLVCDTGMWDRKTPAITSSLRGLAYFEVKVTCADRDLHSGFFGGAARNPIHVLAKIIAD
LHDADGRVTIPGFYEGVHERPPEVLEQWRGLGLTPETLLGPIGLKEPAGERGRMPIELVQSRPSCDANGIVGGYTGEGTK
TVIASTASAKVSFRLVDDQDPKRLAESFEAFVRARVPADCQVEVITYKGSRAISLPFDMPQLGAAKAALQDEWGVPAVTV
GAGGSIPIVGDFKRILGRDTLLIGFGLDDDRIHSPNEKYDLTSFHKGTRSWARILAALGQG
>Mature_460_residues
AALDAILNDIDTDLENALERLFAFLRIPSISTDPAYAGPCREAAAWLAKDLTALGFETSVEETSLHPVVLAHKPKPGAPH
VLFYGHYDVQPVDPLDLWTTPPFEPRIAEDGRGGKTIVGRGASDDKGQVMTFVEACRATIAMTGELPCGVTILIEGAEES
GSQGLPEWVAANRERLGCDVVLVCDTGMWDRKTPAITSSLRGLAYFEVKVTCADRDLHSGFFGGAARNPIHVLAKIIADL
HDADGRVTIPGFYEGVHERPPEVLEQWRGLGLTPETLLGPIGLKEPAGERGRMPIELVQSRPSCDANGIVGGYTGEGTKT
VIASTASAKVSFRLVDDQDPKRLAESFEAFVRARVPADCQVEVITYKGSRAISLPFDMPQLGAAKAALQDEWGVPAVTVG
AGGSIPIVGDFKRILGRDTLLIGFGLDDDRIHSPNEKYDLTSFHKGTRSWARILAALGQG

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=477, Percent_Identity=29.979035639413, Blast_Score=205, Evalue=6e-53,
Organism=Homo sapiens, GI21071039, Length=401, Percent_Identity=32.1695760598504, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI271398277, Length=325, Percent_Identity=30.1538461538462, Blast_Score=152, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17563146, Length=481, Percent_Identity=30.1455301455301, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI193211059, Length=483, Percent_Identity=29.1925465838509, Blast_Score=189, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321159, Length=474, Percent_Identity=30.379746835443, Blast_Score=203, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6319758, Length=472, Percent_Identity=24.7881355932203, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24585880, Length=484, Percent_Identity=31.198347107438, Blast_Score=205, Evalue=7e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 49517; Mature: 49386

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALDAILNDIDTDLENALERLFAFLRIPSISTDPAYAGPCREAAAWLAKDLTALGFETS
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
VEETSLHPVVLAHKPKPGAPHVLFYGHYDVQPVDPLDLWTTPPFEPRIAEDGRGGKTIVG
CCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
RGASDDKGQVMTFVEACRATIAMTGELPCGVTILIEGAEESGSQGLPEWVAANRERLGCD
CCCCCCCCHHHHHHHHHHHHEEEECCCCCCEEEEEECCCCCCCCCCHHHHHCCCHHCCCC
VVLVCDTGMWDRKTPAITSSLRGLAYFEVKVTCADRDLHSGFFGGAARNPIHVLAKIIAD
EEEEECCCCCCCCCCHHHHHCCCEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
LHDADGRVTIPGFYEGVHERPPEVLEQWRGLGLTPETLLGPIGLKEPAGERGRMPIELVQ
HHCCCCEEECCHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHH
SRPSCDANGIVGGYTGEGTKTVIASTASAKVSFRLVDDQDPKRLAESFEAFVRARVPADC
CCCCCCCCCEEECCCCCCCEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCC
QVEVITYKGSRAISLPFDMPQLGAAKAALQDEWGVPAVTVGAGGSIPIVGDFKRILGRDT
EEEEEEECCCEEEECCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCCE
LLIGFGLDDDRIHSPNEKYDLTSFHKGTRSWARILAALGQG
EEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AALDAILNDIDTDLENALERLFAFLRIPSISTDPAYAGPCREAAAWLAKDLTALGFETS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
VEETSLHPVVLAHKPKPGAPHVLFYGHYDVQPVDPLDLWTTPPFEPRIAEDGRGGKTIVG
CCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
RGASDDKGQVMTFVEACRATIAMTGELPCGVTILIEGAEESGSQGLPEWVAANRERLGCD
CCCCCCCCHHHHHHHHHHHHEEEECCCCCCEEEEEECCCCCCCCCCHHHHHCCCHHCCCC
VVLVCDTGMWDRKTPAITSSLRGLAYFEVKVTCADRDLHSGFFGGAARNPIHVLAKIIAD
EEEEECCCCCCCCCCHHHHHCCCEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
LHDADGRVTIPGFYEGVHERPPEVLEQWRGLGLTPETLLGPIGLKEPAGERGRMPIELVQ
HHCCCCEEECCHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHH
SRPSCDANGIVGGYTGEGTKTVIASTASAKVSFRLVDDQDPKRLAESFEAFVRARVPADC
CCCCCCCCCEEECCCCCCCEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCC
QVEVITYKGSRAISLPFDMPQLGAAKAALQDEWGVPAVTVGAGGSIPIVGDFKRILGRDT
EEEEEEECCCEEEECCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCCE
LLIGFGLDDDRIHSPNEKYDLTSFHKGTRSWARILAALGQG
EEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA