Click here to switch to the map view.

The map label for this gene is aroE

Identifier: 170750675

GI number: 170750675

Start: 4535890

End: 4536738

Strand: Reverse

Name: aroE

Synonym: Mrad2831_4285

Alternate gene names: 170750675

Gene position: 4536738-4535890 (Counterclockwise)

Preceding gene: 170750676

Following gene: 170750674

Centisome position: 74.64

GC content: 78.33

Gene sequence:

>849_bases
GTGAGCGTCGCGACCCCCCGCGCCTTCGTGGTCGGCCACCCGATCGCCCACTCGCGCTCGCCGCTGATCCACGGCCACTG
GCTCGCCGTGCACGGGATCCCGGGCTCCTACGAGCGGCTCGACGTGCCGCCGGCGGCGTTCCCGGACTTCGTCCGGGGCC
TGCCGGATTCGGGCTTCCGGGGCGGCAACGTCACGATCCCCCACAAGGAGGCGGCCTTCGCGCTGGCCGACACCCTGACG
CCGCGGGCCCGGGCGATCGGCGCGGTCAACACCCTGGTGGTCGGGCCGGACGGCCGGATCCGCGGCGACAACACCGACGC
GCCGGGCTTCTGCGCCCATCTCGACCACAGCCTGGGCGCGGCCTGGCCGGAACGCGCGGGCGGCACCGCCCTGGTGCTCG
GCGCCGGCGGCGCGGCGCGGGCGGTCGTGGTCGGGCTCGCCGAGCGCGGCCTGCGGCGCGTCTGGGTCGCCAACCGCACC
GCGGCCCGGGCCGAGGCGGTGGCGGCGCTCGCGCCGGGCGTCGGCGCGGCGCTCGCCTGGGACGACCTGCCGGCGGCGCT
TCCCGGGACCGGCCTCCTCGTCAACACCACGTCCCTGGGCATGAAGGGCCAGCCGCCCCTCGCCGTCGACCTGTCGCCCC
TGCCGGCGGACGCGGCCGTGGCCGACATCGTCTACGCGCCCCTGGAGACGGCGCTCCTGGCGGCGGCGCGCCGGCGGGGG
CTCGCCGCCGTGGACGGGCTCGGCATGCTGCTGCACCAGGCGGTGCCGGGTTTCGAGGCGTGGTTCGGCCCGCGCCCCCG
CGTCACCCCGGAGCTGCGCGACCGGATCGTGGCGGACCTCGCTGGATGA

Upstream 100 bases:

>100_bases
CCGCCGAGGGCCTGCGCGCTCGGTTCGGCATCGGCCACGCCACCCTCCAGGTCGAGGAGGCCGGCGGTCCGGCCTGCCGC
CTCGCGCAGGATTGCGCGGC

Downstream 100 bases:

>100_bases
TGCCGACGGCCGGATCCGGACCCGTCATCCTCGGGCTCACGGGCTCGATCGGCATGGGCAAGTCGGCCACGGCCCGGATG
TTCGCGGAGCGCGGCGTCCC

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSVATPRAFVVGHPIAHSRSPLIHGHWLAVHGIPGSYERLDVPPAAFPDFVRGLPDSGFRGGNVTIPHKEAAFALADTLT
PRARAIGAVNTLVVGPDGRIRGDNTDAPGFCAHLDHSLGAAWPERAGGTALVLGAGGAARAVVVGLAERGLRRVWVANRT
AARAEAVAALAPGVGAALAWDDLPAALPGTGLLVNTTSLGMKGQPPLAVDLSPLPADAAVADIVYAPLETALLAAARRRG
LAAVDGLGMLLHQAVPGFEAWFGPRPRVTPELRDRIVADLAG

Sequences:

>Translated_282_residues
MSVATPRAFVVGHPIAHSRSPLIHGHWLAVHGIPGSYERLDVPPAAFPDFVRGLPDSGFRGGNVTIPHKEAAFALADTLT
PRARAIGAVNTLVVGPDGRIRGDNTDAPGFCAHLDHSLGAAWPERAGGTALVLGAGGAARAVVVGLAERGLRRVWVANRT
AARAEAVAALAPGVGAALAWDDLPAALPGTGLLVNTTSLGMKGQPPLAVDLSPLPADAAVADIVYAPLETALLAAARRRG
LAAVDGLGMLLHQAVPGFEAWFGPRPRVTPELRDRIVADLAG
>Mature_281_residues
SVATPRAFVVGHPIAHSRSPLIHGHWLAVHGIPGSYERLDVPPAAFPDFVRGLPDSGFRGGNVTIPHKEAAFALADTLTP
RARAIGAVNTLVVGPDGRIRGDNTDAPGFCAHLDHSLGAAWPERAGGTALVLGAGGAARAVVVGLAERGLRRVWVANRTA
ARAEAVAALAPGVGAALAWDDLPAALPGTGLLVNTTSLGMKGQPPLAVDLSPLPADAAVADIVYAPLETALLAAARRRGL
AAVDGLGMLLHQAVPGFEAWFGPRPRVTPELRDRIVADLAG

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1789675, Length=268, Percent_Identity=39.1791044776119, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI1787983, Length=267, Percent_Identity=33.7078651685393, Blast_Score=128, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6320332, Length=281, Percent_Identity=30.6049822064057, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_METRJ (B1M3B2)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001756935.1
- ProteinModelPortal:   B1M3B2
- SMR:   B1M3B2
- GeneID:   6140344
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_4285
- HOGENOM:   HBG553408
- OMA:   ESVPPEG
- ProtClustDB:   CLSK2330698
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 28926; Mature: 28795

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: ACT_SITE 69-69

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVATPRAFVVGHPIAHSRSPLIHGHWLAVHGIPGSYERLDVPPAAFPDFVRGLPDSGFR
CCCCCCCEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCC
GGNVTIPHKEAAFALADTLTPRARAIGAVNTLVVGPDGRIRGDNTDAPGFCAHLDHSLGA
CCCEECCCHHHHHHHHHHCCHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCC
AWPERAGGTALVLGAGGAARAVVVGLAERGLRRVWVANRTAARAEAVAALAPGVGAALAW
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCEEEC
DDLPAALPGTGLLVNTTSLGMKGQPPLAVDLSPLPADAAVADIVYAPLETALLAAARRRG
CCCCCCCCCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
LAAVDGLGMLLHQAVPGFEAWFGPRPRVTPELRDRIVADLAG
CHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SVATPRAFVVGHPIAHSRSPLIHGHWLAVHGIPGSYERLDVPPAAFPDFVRGLPDSGFR
CCCCCCEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCC
GGNVTIPHKEAAFALADTLTPRARAIGAVNTLVVGPDGRIRGDNTDAPGFCAHLDHSLGA
CCCEECCCHHHHHHHHHHCCHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCC
AWPERAGGTALVLGAGGAARAVVVGLAERGLRRVWVANRTAARAEAVAALAPGVGAALAW
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCEEEC
DDLPAALPGTGLLVNTTSLGMKGQPPLAVDLSPLPADAAVADIVYAPLETALLAAARRRG
CCCCCCCCCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
LAAVDGLGMLLHQAVPGFEAWFGPRPRVTPELRDRIVADLAG
CHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA