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The map label for this gene is nemA [H]

Identifier: 170750372

GI number: 170750372

Start: 4227604

End: 4228695

Strand: Reverse

Name: nemA [H]

Synonym: Mrad2831_3975

Alternate gene names: 170750372

Gene position: 4228695-4227604 (Counterclockwise)

Preceding gene: 170750373

Following gene: 170750371

Centisome position: 69.58

GC content: 71.25

Gene sequence:

>1092_bases
ATGCCGTCCCTGCTCGACCCGATCCGCATCGGCGCCATCGAGGCGAAGAACCGGATCTTCATGGCTCCGCTGACCCGCGG
CCGCGGCACCCGCGAGCACGTTCCGACCCCGATCATGGCCGAGTACTACGCGCAGCGCGCCGGTGCCGGCCTGATCCTCA
CCGAGGCCACCGGCATCAGCCAGGAGGGCCTCGGCTGGCCCTACGCGCCGGGCATCTGGTCCGACGCCCAGGTCGACGCG
TGGCGGCCGATCGTGAAGGCCGTTCACGACCGGGGCGGCAAGATCGTCTGCCAGATCTGGCACATGGGGCGGATGGTCCA
TTCGGACTTCCTCGGCGGCGCCAAGCCGGTCTCGTCCTCGCCCACGACGGCCCCCGACCAGGCCCACACCTATTCCGGCA
AGAAGCCCTACAGCGAGGCCCGCGCCCTGGAGGTGTCCGAGATCCCGCGCCTGCTCGCCGATTACGAGCGGGCCACCGCC
AACGCCCTGGCGGCCGGGTTCGATGGGGTGCAGATCCACGCCGCGAACGGCTACCTGATCGACGAGTTCCTCCGCGACGG
CACCAACCACCGCACCGACCAGTACGGCGGCTCCATCGAGAACCGCGTGCGACTCCTGCGCGAGGTCACCGAGGCGGTGG
CGAAGGTCGCCGGGCCGGAGCGCACGGGCGTGCGCCTCTCGCCGAACGGCGCGATCCAGGGCTGCAACGACTCGAACCCG
GAGCCGCTGTTCGTCGCCGCCGCCCAGGCGCTCGCCGATGCCGGGATCGCCTTCCTGGAGATGCGCGAGCCGGGACCGAA
CGGCACCTTCGGCAAGGCCGACCACCCGCCGATCGCCCCGGCGATGCGGCGCGTGTTCCGCAACCCGATGATCCTGAACG
CCGACTATGACGGGATCAGCGGCCAGAAGGCCCTGGATGCCGGCGAGGCCGACGCCATCGCGTTCGGCCGGACCTTCATC
GCCAATCCGGACCTCGTCGAGCGCATCGCCGAGGGCGCGCCGCTCAACAAGGACGACGCCGCGACGTGGTACAGCCAGGG
GCCCGAGGGCTACGTCGACTATCCGACGCTGGCCGAGTCCCGCGCCGCCTGA

Upstream 100 bases:

>100_bases
GTCCTGCGGCGCAACACCTTGGCATCGCGGCGCCGCAGCGCCACCTCGGCGCCGGATTAAACCCGGACGCGGCCCCCGCC
GCGCCAGCACGAGGTAACCC

Downstream 100 bases:

>100_bases
CGCGCCGCCTGACGCGCCGTCTTCGGGACCCCTGCCGCATCCGCGCGGGTGCGGCAGGGGTGCGGCAGGGGTGCGGCAGG
AAAGCGCGGCGGTTCCTTGG

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MPSLLDPIRIGAIEAKNRIFMAPLTRGRGTREHVPTPIMAEYYAQRAGAGLILTEATGISQEGLGWPYAPGIWSDAQVDA
WRPIVKAVHDRGGKIVCQIWHMGRMVHSDFLGGAKPVSSSPTTAPDQAHTYSGKKPYSEARALEVSEIPRLLADYERATA
NALAAGFDGVQIHAANGYLIDEFLRDGTNHRTDQYGGSIENRVRLLREVTEAVAKVAGPERTGVRLSPNGAIQGCNDSNP
EPLFVAAAQALADAGIAFLEMREPGPNGTFGKADHPPIAPAMRRVFRNPMILNADYDGISGQKALDAGEADAIAFGRTFI
ANPDLVERIAEGAPLNKDDAATWYSQGPEGYVDYPTLAESRAA

Sequences:

>Translated_363_residues
MPSLLDPIRIGAIEAKNRIFMAPLTRGRGTREHVPTPIMAEYYAQRAGAGLILTEATGISQEGLGWPYAPGIWSDAQVDA
WRPIVKAVHDRGGKIVCQIWHMGRMVHSDFLGGAKPVSSSPTTAPDQAHTYSGKKPYSEARALEVSEIPRLLADYERATA
NALAAGFDGVQIHAANGYLIDEFLRDGTNHRTDQYGGSIENRVRLLREVTEAVAKVAGPERTGVRLSPNGAIQGCNDSNP
EPLFVAAAQALADAGIAFLEMREPGPNGTFGKADHPPIAPAMRRVFRNPMILNADYDGISGQKALDAGEADAIAFGRTFI
ANPDLVERIAEGAPLNKDDAATWYSQGPEGYVDYPTLAESRAA
>Mature_362_residues
PSLLDPIRIGAIEAKNRIFMAPLTRGRGTREHVPTPIMAEYYAQRAGAGLILTEATGISQEGLGWPYAPGIWSDAQVDAW
RPIVKAVHDRGGKIVCQIWHMGRMVHSDFLGGAKPVSSSPTTAPDQAHTYSGKKPYSEARALEVSEIPRLLADYERATAN
ALAAGFDGVQIHAANGYLIDEFLRDGTNHRTDQYGGSIENRVRLLREVTEAVAKVAGPERTGVRLSPNGAIQGCNDSNPE
PLFVAAAQALADAGIAFLEMREPGPNGTFGKADHPPIAPAMRRVFRNPMILNADYDGISGQKALDAGEADAIAFGRTFIA
NPDLVERIAEGAPLNKDDAATWYSQGPEGYVDYPTLAESRAA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=362, Percent_Identity=44.1988950276243, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1789463, Length=346, Percent_Identity=30.0578034682081, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17559804, Length=371, Percent_Identity=26.9541778975741, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=359, Percent_Identity=26.7409470752089, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=356, Percent_Identity=25.561797752809, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17565138, Length=350, Percent_Identity=25.7142857142857, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI72001454, Length=352, Percent_Identity=26.4204545454545, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17540738, Length=352, Percent_Identity=25.5681818181818, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17566914, Length=225, Percent_Identity=28.8888888888889, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=380, Percent_Identity=36.3157894736842, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6321973, Length=379, Percent_Identity=36.4116094986807, Blast_Score=209, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38998; Mature: 38867

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLLDPIRIGAIEAKNRIFMAPLTRGRGTREHVPTPIMAEYYAQRAGAGLILTEATGIS
CCCCCCCHHCCEEECCCEEEEEECCCCCCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCC
QEGLGWPYAPGIWSDAQVDAWRPIVKAVHDRGGKIVCQIWHMGRMVHSDFLGGAKPVSSS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCC
PTTAPDQAHTYSGKKPYSEARALEVSEIPRLLADYERATANALAAGFDGVQIHAANGYLI
CCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHH
DEFLRDGTNHRTDQYGGSIENRVRLLREVTEAVAKVAGPERTGVRLSPNGAIQGCNDSNP
HHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEECCCCCCC
EPLFVAAAQALADAGIAFLEMREPGPNGTFGKADHPPIAPAMRRVFRNPMILNADYDGIS
CCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCC
GQKALDAGEADAIAFGRTFIANPDLVERIAEGAPLNKDDAATWYSQGPEGYVDYPTLAES
CCHHHCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCHHC
RAA
CCC
>Mature Secondary Structure 
PSLLDPIRIGAIEAKNRIFMAPLTRGRGTREHVPTPIMAEYYAQRAGAGLILTEATGIS
CCCCCCHHCCEEECCCEEEEEECCCCCCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCC
QEGLGWPYAPGIWSDAQVDAWRPIVKAVHDRGGKIVCQIWHMGRMVHSDFLGGAKPVSSS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCC
PTTAPDQAHTYSGKKPYSEARALEVSEIPRLLADYERATANALAAGFDGVQIHAANGYLI
CCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHH
DEFLRDGTNHRTDQYGGSIENRVRLLREVTEAVAKVAGPERTGVRLSPNGAIQGCNDSNP
HHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEECCCCCCC
EPLFVAAAQALADAGIAFLEMREPGPNGTFGKADHPPIAPAMRRVFRNPMILNADYDGIS
CCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCC
GQKALDAGEADAIAFGRTFIANPDLVERIAEGAPLNKDDAATWYSQGPEGYVDYPTLAES
CCHHHCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCHHC
RAA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]