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The map label for this gene is yngG [H]

Identifier: 170750267

GI number: 170750267

Start: 4119507

End: 4120433

Strand: Reverse

Name: yngG [H]

Synonym: Mrad2831_3870

Alternate gene names: 170750267

Gene position: 4120433-4119507 (Counterclockwise)

Preceding gene: 170750268

Following gene: 170750266

Centisome position: 67.79

GC content: 73.68

Gene sequence:

>927_bases
ATGAGCGGCGATGCGCTGATCGTCCAGGAGGTCGCGACCCGGGACGGCTTCCAGATCGAGCCGGTCTTCGTGCCGACCCC
GGAGAAGATCCGCCTGATCGACGCGCTCTCGGAGGCCGGCTTCAGCCGGATCGAGGTCTCCTCCTTCGTCTCGCCGAAGG
CGGTGCCGGCCCTCGCCGACGCGGCCGAGGTCTTTTCCGGGATCCGGCGCCGGCCCGGGACCGTCTACGTCGCCCTGGTG
CCGAACCCGAAGGGCGCCGAGCGGGCGCTCGCCGCCAGGGTCGACGAGATCAACCTCGTCGCCTCTGTGAGCGAGACCCA
CAACCGCGCCAACATGGGCATGAGTCCGGCCGACTCGATCGCCGGCTTCGCGCGGATCATGGATTCGGTGCGCGGCGCCC
CCGTCAGCACCAACGCCACGGTGGCGACGGCTTTCGGCTGCCCATTCGAGGGCGACCAGCCCGCCGACACGGTCGTCGCG
CAGGTGGAGCGCTACCTGTCGCTGGGCGTCGACGGCGTGACGCTGGCCGACACGACCGGCATGGCCAATCCCCGGCAGGT
GAGCCGCCTCGTCGCGCGGGTCCTGCCCCTGGTCGGTCCCGGGCGCCTGACGCTCCACTTCCACAACACCCGCGGCCTCG
GCCTCGCCAACGTGCTGGCGGCCTACGACGCGGGCGCCCGGCGCTTCGACGCGGCGCTCGGCGGCCTGGGCGGCTGCCCG
TTCGCGCCCGGCGCCACCGGCAACATCTGCACCGAGGATCTCGTCAGCATGGCCCACGAGATGGGCGTGCCGACGGGCCT
CGACCTCCCGGCGCTGATCGGCCTGTCGCGCGACCTGCCGCGCCTCGTCGGCCACGACGTGCCCGGCCAGGTCGCCAAGG
CCGGACGGCCCTGCGACCTGCACCCGGCGCCCGCCCGGGCGGCCTGA

Upstream 100 bases:

>100_bases
GGCAGGGGCCGGCCCTCGGCGCCCACACCGATTCCGTGCTCGCCGAGCTCGGTCTCGACCCGGCGCGCATCGCCGCGCTG
CGCGAGAAGGGAGCCGTGGC

Downstream 100 bases:

>100_bases
GCCGGAGACGGAGAGGGGCGGCCTTACAATGGCCCGCCCCACGCGTCGGACGCGACGCGCTCCCTCTCCCGAACGGGAGA
GGGTCGGGGTGAGGGATGAG

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSGDALIVQEVATRDGFQIEPVFVPTPEKIRLIDALSEAGFSRIEVSSFVSPKAVPALADAAEVFSGIRRRPGTVYVALV
PNPKGAERALAARVDEINLVASVSETHNRANMGMSPADSIAGFARIMDSVRGAPVSTNATVATAFGCPFEGDQPADTVVA
QVERYLSLGVDGVTLADTTGMANPRQVSRLVARVLPLVGPGRLTLHFHNTRGLGLANVLAAYDAGARRFDAALGGLGGCP
FAPGATGNICTEDLVSMAHEMGVPTGLDLPALIGLSRDLPRLVGHDVPGQVAKAGRPCDLHPAPARAA

Sequences:

>Translated_308_residues
MSGDALIVQEVATRDGFQIEPVFVPTPEKIRLIDALSEAGFSRIEVSSFVSPKAVPALADAAEVFSGIRRRPGTVYVALV
PNPKGAERALAARVDEINLVASVSETHNRANMGMSPADSIAGFARIMDSVRGAPVSTNATVATAFGCPFEGDQPADTVVA
QVERYLSLGVDGVTLADTTGMANPRQVSRLVARVLPLVGPGRLTLHFHNTRGLGLANVLAAYDAGARRFDAALGGLGGCP
FAPGATGNICTEDLVSMAHEMGVPTGLDLPALIGLSRDLPRLVGHDVPGQVAKAGRPCDLHPAPARAA
>Mature_307_residues
SGDALIVQEVATRDGFQIEPVFVPTPEKIRLIDALSEAGFSRIEVSSFVSPKAVPALADAAEVFSGIRRRPGTVYVALVP
NPKGAERALAARVDEINLVASVSETHNRANMGMSPADSIAGFARIMDSVRGAPVSTNATVATAFGCPFEGDQPADTVVAQ
VERYLSLGVDGVTLADTTGMANPRQVSRLVARVLPLVGPGRLTLHFHNTRGLGLANVLAAYDAGARRFDAALGGLGGCPF
APGATGNICTEDLVSMAHEMGVPTGLDLPALIGLSRDLPRLVGHDVPGQVAKAGRPCDLHPAPARAA

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI109150422, Length=287, Percent_Identity=37.9790940766551, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI62198232, Length=293, Percent_Identity=38.9078498293515, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI109150427, Length=287, Percent_Identity=37.9790940766551, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI260654708, Length=86, Percent_Identity=53.4883720930233, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17510563, Length=297, Percent_Identity=37.7104377104377, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6320019, Length=233, Percent_Identity=26.1802575107296, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24582381, Length=289, Percent_Identity=38.0622837370242, Blast_Score=205, Evalue=3e-53,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 31913; Mature: 31782

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGDALIVQEVATRDGFQIEPVFVPTPEKIRLIDALSEAGFSRIEVSSFVSPKAVPALAD
CCCCHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEHHHHCCCCHHHHHHH
AAEVFSGIRRRPGTVYVALVPNPKGAERALAARVDEINLVASVSETHNRANMGMSPADSI
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHEEHHHHHHHHHHCCCCHHHHH
AGFARIMDSVRGAPVSTNATVATAFGCPFEGDQPADTVVAQVERYLSLGVDGVTLADTTG
HHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCC
MANPRQVSRLVARVLPLVGPGRLTLHFHNTRGLGLANVLAAYDAGARRFDAALGGLGGCP
CCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCC
FAPGATGNICTEDLVSMAHEMGVPTGLDLPALIGLSRDLPRLVGHDVPGQVAKAGRPCDL
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHCCCCCCHHHHCCCCCCC
HPAPARAA
CCCCCCCC
>Mature Secondary Structure 
SGDALIVQEVATRDGFQIEPVFVPTPEKIRLIDALSEAGFSRIEVSSFVSPKAVPALAD
CCCHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEHHHHCCCCHHHHHHH
AAEVFSGIRRRPGTVYVALVPNPKGAERALAARVDEINLVASVSETHNRANMGMSPADSI
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHEEHHHHHHHHHHCCCCHHHHH
AGFARIMDSVRGAPVSTNATVATAFGCPFEGDQPADTVVAQVERYLSLGVDGVTLADTTG
HHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCC
MANPRQVSRLVARVLPLVGPGRLTLHFHNTRGLGLANVLAAYDAGARRFDAALGGLGGCP
CCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCC
FAPGATGNICTEDLVSMAHEMGVPTGLDLPALIGLSRDLPRLVGHDVPGQVAKAGRPCDL
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHCCCCCCHHHHCCCCCCC
HPAPARAA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]