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The map label for this gene is pitA [H]

Identifier: 170750263

GI number: 170750263

Start: 4114664

End: 4116292

Strand: Direct

Name: pitA [H]

Synonym: Mrad2831_3866

Alternate gene names: 170750263

Gene position: 4114664-4116292 (Clockwise)

Preceding gene: 170750256

Following gene: 170750271

Centisome position: 67.7

GC content: 70.9

Gene sequence:

>1629_bases
ATGTCCGATTCCGCCGTGATGCCGTCGGGGGCGGTGGGAGGCCGGCGGGCGGGCGGTCCGCGCCTCGACCACGGGACGCA
TCTCGGCGGGGTGGTCGCGTTCCTCCTCGTCCTGGTGGCGGGCCTCGGCTACGCGGTGGTGAGCCTCGTCGCCGACATGA
ACGCGGTCGGCGAGGCGCCCCTGGCCTACGGCGCCTTCGCGCTGCTCGGCCTCGCCCTGCTGATCGCCCTCGCCTTCGAG
TTCGTGAACGGCTTCCACGACACCGCCAACGCGGTCGCCACGGTGATCTACACCCACTCGATGCCGCCGCTCGTCGCGGT
GATCTGGTCGGGTGCCTTCAACTTCCTGGGCGTGATGCTGTCCTCGGGCGCGGTGGCCTACGCGATCGTGACGCTGCTGC
CCGTCGAGCTGATCCTCAACGTCGGCTCGCACGCGGGCTACGCGATGATCTTCGCGCTCCTGCTGGCGGCCATCATCTGG
AACCTCGGGACCTGGGCCTTCGGGCTGCCGAACTCGTCGTCCCACGCGCTGATCGGCTCGGTGCTCGGCGTCGGCCTCGC
CAACCAGCTGATGAACGGCGGCGACGGTACCGCGGGCGTCGACTGGACCCAGGCGCTCAACGTCTTCAAGGCGCTGCTGT
TCTCGCCGGTCTGCGGCTTCGTGCTCGCGGCGCTGCTGCTGCTCGCCCTGAAATTCACGGTGCGCCGCAGGGACCTCTAC
GAGGCGCCCGAGGGCGACGCGCCCCCGCCCCTGTGGATCCGCGGCCTGCTGATCCTCACCTGCACGCTGGTCTCGTTCTT
CCACGGCGGCAACGACGGGCAGAAGGGCATGGGCCTGATCATGCTGATCCTGATCGGCGCCGCCCCCACCGCCTACGCGC
TCAACCGCACCATGGCGGACGACACGACGCCCGCCTTCGTGCAGGCCTCGACGGCGGCGAGCGCGGTGTTCTCCGCCCGC
GCCCCCGGCGCGGCGGTTCCGGACGCCGCCGCCGCGCGCCGGACGGTGACGGAGGCGCTCAAGACCAAGGCGCTGAACAC
GCCGCCCGTCTACGCGGCCCTGGCGGCGCTCTCCGACGACATCGCCGCCAGCGTCAAGAATTACGGGGCGATCCGGACGG
TGCCGGCCGCCCAGACCCAGAACCTGCGCAGCGACATGTATCTCGCCGCCGACGCGGTGCGCGTGCTGCCGAGCGCCGGG
GCCGACTTCTCGGAGACCGACAAGGCGGACCTGAAACGCTACGCGGGCCTGCTGAACGACGGCACGCGCTACATCCCGGG
CTGGGTGAAGGTCTGCGTCGCCCTGGCGCTGGGTCTCGGCACCATGGTCGGATGGAAGCGCATCGTCGTCACGGTGGGCG
AGAAGATCGGCAAGACCCACCTGACCTACGCGCAGGGCGCCTCGGCCGAGATCGTCGCCGCCGGCACGATCGGGCTGGCC
GAACTCTACGGCCTGCCGGTCTCGACGACCCACATCCTGTCCTCCGGCGTCGCCGGCACCATGGCGGCCAACGGCTCGGG
ACTCCAGATGTCCACGGTGCGCAACATGGCGCTCGCCTGGGTCCTGACCCTGCCGGCGGCCATCACCCTCGCGGCCGTGC
TGTTCTTCATCCTGCGCCACGTGTTCTGA

Upstream 100 bases:

>100_bases
GCCGTCACGGGCCGTCACGGGCCTGCCGTCGCCCGTCACCCAACCGTCACCGCTCCATCTTAATCCCGGCCCACCACCAC
GTAGAATTCCGCGAGGGTTC

Downstream 100 bases:

>100_bases
CGCGGGCTCGGCCCGGGCGGGCCGGAGACCGCGGTCCGCCTCCGACGAGGCGGCCCGGTCTCCGGCTTCCTGCAGCGCCC
CCCGACGCTTCAGGAACTCG

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MSDSAVMPSGAVGGRRAGGPRLDHGTHLGGVVAFLLVLVAGLGYAVVSLVADMNAVGEAPLAYGAFALLGLALLIALAFE
FVNGFHDTANAVATVIYTHSMPPLVAVIWSGAFNFLGVMLSSGAVAYAIVTLLPVELILNVGSHAGYAMIFALLLAAIIW
NLGTWAFGLPNSSSHALIGSVLGVGLANQLMNGGDGTAGVDWTQALNVFKALLFSPVCGFVLAALLLLALKFTVRRRDLY
EAPEGDAPPPLWIRGLLILTCTLVSFFHGGNDGQKGMGLIMLILIGAAPTAYALNRTMADDTTPAFVQASTAASAVFSAR
APGAAVPDAAAARRTVTEALKTKALNTPPVYAALAALSDDIAASVKNYGAIRTVPAAQTQNLRSDMYLAADAVRVLPSAG
ADFSETDKADLKRYAGLLNDGTRYIPGWVKVCVALALGLGTMVGWKRIVVTVGEKIGKTHLTYAQGASAEIVAAGTIGLA
ELYGLPVSTTHILSSGVAGTMAANGSGLQMSTVRNMALAWVLTLPAAITLAAVLFFILRHVF

Sequences:

>Translated_542_residues
MSDSAVMPSGAVGGRRAGGPRLDHGTHLGGVVAFLLVLVAGLGYAVVSLVADMNAVGEAPLAYGAFALLGLALLIALAFE
FVNGFHDTANAVATVIYTHSMPPLVAVIWSGAFNFLGVMLSSGAVAYAIVTLLPVELILNVGSHAGYAMIFALLLAAIIW
NLGTWAFGLPNSSSHALIGSVLGVGLANQLMNGGDGTAGVDWTQALNVFKALLFSPVCGFVLAALLLLALKFTVRRRDLY
EAPEGDAPPPLWIRGLLILTCTLVSFFHGGNDGQKGMGLIMLILIGAAPTAYALNRTMADDTTPAFVQASTAASAVFSAR
APGAAVPDAAAARRTVTEALKTKALNTPPVYAALAALSDDIAASVKNYGAIRTVPAAQTQNLRSDMYLAADAVRVLPSAG
ADFSETDKADLKRYAGLLNDGTRYIPGWVKVCVALALGLGTMVGWKRIVVTVGEKIGKTHLTYAQGASAEIVAAGTIGLA
ELYGLPVSTTHILSSGVAGTMAANGSGLQMSTVRNMALAWVLTLPAAITLAAVLFFILRHVF
>Mature_541_residues
SDSAVMPSGAVGGRRAGGPRLDHGTHLGGVVAFLLVLVAGLGYAVVSLVADMNAVGEAPLAYGAFALLGLALLIALAFEF
VNGFHDTANAVATVIYTHSMPPLVAVIWSGAFNFLGVMLSSGAVAYAIVTLLPVELILNVGSHAGYAMIFALLLAAIIWN
LGTWAFGLPNSSSHALIGSVLGVGLANQLMNGGDGTAGVDWTQALNVFKALLFSPVCGFVLAALLLLALKFTVRRRDLYE
APEGDAPPPLWIRGLLILTCTLVSFFHGGNDGQKGMGLIMLILIGAAPTAYALNRTMADDTTPAFVQASTAASAVFSARA
PGAAVPDAAAARRTVTEALKTKALNTPPVYAALAALSDDIAASVKNYGAIRTVPAAQTQNLRSDMYLAADAVRVLPSAGA
DFSETDKADLKRYAGLLNDGTRYIPGWVKVCVALALGLGTMVGWKRIVVTVGEKIGKTHLTYAQGASAEIVAAGTIGLAE
LYGLPVSTTHILSSGVAGTMAANGSGLQMSTVRNMALAWVLTLPAAITLAAVLFFILRHVF

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=486, Percent_Identity=42.5925925925926, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI1789360, Length=479, Percent_Identity=42.3799582463466, Blast_Score=349, Evalue=3e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 55887; Mature: 55756

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HC
>Mature Secondary Structure 
SDSAVMPSGAVGGRRAGGPRLDHGTHLGGVVAFLLVLVAGLGYAVVSLVADMNAVGEAP
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LAYGAFALLGLALLIALAFEFVNGFHDTANAVATVIYTHSMPPLVAVIWSGAFNFLGVML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
SSGAVAYAIVTLLPVELILNVGSHAGYAMIFALLLAAIIWNLGTWAFGLPNSSSHALIGS
HCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHH
VLGVGLANQLMNGGDGTAGVDWTQALNVFKALLFSPVCGFVLAALLLLALKFTVRRRDLY
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAPEGDAPPPLWIRGLLILTCTLVSFFHGGNDGQKGMGLIMLILIGAAPTAYALNRTMAD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCC
DTTPAFVQASTAASAVFSARAPGAAVPDAAAARRTVTEALKTKALNTPPVYAALAALSDD
CCCCHHEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
IAASVKNYGAIRTVPAAQTQNLRSDMYLAADAVRVLPSAGADFSETDKADLKRYAGLLND
HHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
GTRYIPGWVKVCVALALGLGTMVGWKRIVVTVGEKIGKTHLTYAQGASAEIVAAGTIGLA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEECCHHHH
ELYGLPVSTTHILSSGVAGTMAANGSGLQMSTVRNMALAWVLTLPAAITLAAVLFFILRH
HHHCCCCCHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VF
HC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]