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The map label for this gene is mmsB [H]

Identifier: 170750180

GI number: 170750180

Start: 4022182

End: 4023075

Strand: Reverse

Name: mmsB [H]

Synonym: Mrad2831_3782

Alternate gene names: 170750180

Gene position: 4023075-4022182 (Counterclockwise)

Preceding gene: 170750181

Following gene: 170750179

Centisome position: 66.19

GC content: 72.37

Gene sequence:

>894_bases
ATGGCACAGACCATCGGGTTCATCGGTCTCGGCAACATGGGCGGGCCCATGGCGGCCAACCTCGTGAAGGCGGGTCACGC
GGTGCGCGGCTTCGATCTCGTACCGGCCTCGCTGGAGGCGGCGCGCGCCGAGGGGATCGCGGTCGCGGGCTCGGCCCTGG
AGGCGGCCGAAGGGGCGGATGTCGTGGTCACCATGCTGCCGGCCGGGCGGCACGTGGTCGAGGTCTGGACGCAGCTCGTC
GAGGCGCTGCCCGCCGGCACGCTGCTGATCGATTCCTCCACGGTGGATGTCGAGAGCGCCCGCAAGGCCCACGCCCTCGC
CGCGGCGCGCGGCTGCCTGTCGGTGGACGCGCCGGTCTCCGGCGGGACCGGCGGCGCGAAGGCCGGAACGCTCACGTTCA
TGGCCGGCGGCGCCGAGGCCGCCTTCGACCGGGCGGAGCCGCTGCTGAAGGCGATGGGCAAGAACGTGTTCCATTGCGGC
GATGCCGGCGCCGGGCAGGCGGCCAAGATCTGCAACAACATGATCCTCGGCATCTCGATGATCGGCGTCAGCGAGGCGTT
CGCGCTCGGCGAGAAGCTCGGCTTGTCGCACCAGGCGCTCTACGACGTCGCCTCGGTCTCCTCGGGCCAGTGCTGGTCGC
TGACCACCTACTGCCCGGTGCCGGGCCCGGTGCCGACCTCGCCGGCCAACAACGGCTACAAGCCGGGCTTCGCCGCGGCG
CTGATGCTCAAGGACCTCCGGCTCGCCCAGGCCGCCGCCATCGCCGCGGGGGCGGCGACGCCGCTGGGCGCCGAGGCCGC
GCAGATCTACGGTCTGTTCGAGGGCGCGGGCCACGGGGGAGAGGACTTTTCCGCGATCATCCGTATGCTTCGCGGGCAGG
GAGGCGAGTCGTGA

Upstream 100 bases:

>100_bases
CCGCTGTGATCCCTGGAGCCCTCCTTCGAGGCCCGCTTCGTCGGCTCAGGACGAGGACGCGGGTGGGAGACGCGAGGGAC
AACAATCAAGGGAGGACGGG

Downstream 100 bases:

>100_bases
GCGAGCCGTACGAGACGATCCTGACGGAGACGCGCGGGCGCGTCCTGCTGATCACCCTCAACCGGCCGAAGGCGCTGAAC
GCCCTCAACGCGCAGCTGAC

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: HIBADH [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MAQTIGFIGLGNMGGPMAANLVKAGHAVRGFDLVPASLEAARAEGIAVAGSALEAAEGADVVVTMLPAGRHVVEVWTQLV
EALPAGTLLIDSSTVDVESARKAHALAAARGCLSVDAPVSGGTGGAKAGTLTFMAGGAEAAFDRAEPLLKAMGKNVFHCG
DAGAGQAAKICNNMILGISMIGVSEAFALGEKLGLSHQALYDVASVSSGQCWSLTTYCPVPGPVPTSPANNGYKPGFAAA
LMLKDLRLAQAAAIAAGAATPLGAEAAQIYGLFEGAGHGGEDFSAIIRMLRGQGGES

Sequences:

>Translated_297_residues
MAQTIGFIGLGNMGGPMAANLVKAGHAVRGFDLVPASLEAARAEGIAVAGSALEAAEGADVVVTMLPAGRHVVEVWTQLV
EALPAGTLLIDSSTVDVESARKAHALAAARGCLSVDAPVSGGTGGAKAGTLTFMAGGAEAAFDRAEPLLKAMGKNVFHCG
DAGAGQAAKICNNMILGISMIGVSEAFALGEKLGLSHQALYDVASVSSGQCWSLTTYCPVPGPVPTSPANNGYKPGFAAA
LMLKDLRLAQAAAIAAGAATPLGAEAAQIYGLFEGAGHGGEDFSAIIRMLRGQGGES
>Mature_296_residues
AQTIGFIGLGNMGGPMAANLVKAGHAVRGFDLVPASLEAARAEGIAVAGSALEAAEGADVVVTMLPAGRHVVEVWTQLVE
ALPAGTLLIDSSTVDVESARKAHALAAARGCLSVDAPVSGGTGGAKAGTLTFMAGGAEAAFDRAEPLLKAMGKNVFHCGD
AGAGQAAKICNNMILGISMIGVSEAFALGEKLGLSHQALYDVASVSSGQCWSLTTYCPVPGPVPTSPANNGYKPGFAAAL
MLKDLRLAQAAAIAAGAATPLGAEAAQIYGLFEGAGHGGEDFSAIIRMLRGQGGES

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=292, Percent_Identity=44.5205479452055, Blast_Score=248, Evalue=4e-66,
Organism=Homo sapiens, GI40556376, Length=291, Percent_Identity=27.8350515463918, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI145693186, Length=285, Percent_Identity=35.7894736842105, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1790315, Length=296, Percent_Identity=33.445945945946, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1786719, Length=293, Percent_Identity=32.4232081911263, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1789092, Length=295, Percent_Identity=30.8474576271186, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17557316, Length=294, Percent_Identity=42.8571428571429, Blast_Score=223, Evalue=7e-59,
Organism=Drosophila melanogaster, GI24655230, Length=293, Percent_Identity=41.9795221843003, Blast_Score=241, Evalue=3e-64,
Organism=Drosophila melanogaster, GI19922568, Length=293, Percent_Identity=41.9795221843003, Blast_Score=241, Evalue=3e-64,
Organism=Drosophila melanogaster, GI28574115, Length=290, Percent_Identity=22.7586206896552, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24655240, Length=66, Percent_Identity=46.969696969697, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR011548
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.31 [H]

Molecular weight: Translated: 29572; Mature: 29441

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQTIGFIGLGNMGGPMAANLVKAGHAVRGFDLVPASLEAARAEGIAVAGSALEAAEGAD
CCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCEEEECCHHHHCCCCC
VVVTMLPAGRHVVEVWTQLVEALPAGTLLIDSSTVDVESARKAHALAAARGCLSVDAPVS
EEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEECCCCC
GGTGGAKAGTLTFMAGGAEAAFDRAEPLLKAMGKNVFHCGDAGAGQAAKICNNMILGISM
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHH
IGVSEAFALGEKLGLSHQALYDVASVSSGQCWSLTTYCPVPGPVPTSPANNGYKPGFAAA
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHH
LMLKDLRLAQAAAIAAGAATPLGAEAAQIYGLFEGAGHGGEDFSAIIRMLRGQGGES
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AQTIGFIGLGNMGGPMAANLVKAGHAVRGFDLVPASLEAARAEGIAVAGSALEAAEGAD
CCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCEEEECCHHHHCCCCC
VVVTMLPAGRHVVEVWTQLVEALPAGTLLIDSSTVDVESARKAHALAAARGCLSVDAPVS
EEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEECCCCC
GGTGGAKAGTLTFMAGGAEAAFDRAEPLLKAMGKNVFHCGDAGAGQAAKICNNMILGISM
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHH
IGVSEAFALGEKLGLSHQALYDVASVSSGQCWSLTTYCPVPGPVPTSPANNGYKPGFAAA
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHH
LMLKDLRLAQAAAIAAGAATPLGAEAAQIYGLFEGAGHGGEDFSAIIRMLRGQGGES
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]