BacMap

Definition	Methylobacterium radiotolerans JCM 2831 chromosome, complete genome.
Accession	NC_010505
Length	6,077,833

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The map label for this gene is echA8 [H]

Identifier: 170750179

GI number: 170750179

Start: 4021406

End: 4022185

Strand: Reverse

Name: echA8 [H]

Synonym: Mrad2831_3781

Alternate gene names: 170750179

Gene position: 4022185-4021406 (Counterclockwise)

Preceding gene: 170750180

Following gene: 170750178

Centisome position: 66.18

GC content: 69.62

Gene sequence:

>780_bases
GTGAGCGAGCCGTACGAGACGATCCTGACGGAGACGCGCGGGCGCGTCCTGCTGATCACCCTCAACCGGCCGAAGGCGCT
GAACGCCCTCAACGCGCAGCTGACCCGAGAGGTGATCCGGGCGGCGACCGAGGCGGATGCCGATCCGGGGATCGGCTGCA
TCGTCGTCACCGGCTCGGCCAAGGCCTTCGCGGCCGGCGCCGACATCAAGGAGATGCAGCACGCCACCTACGCGGAGATG
TACGCGGGCGACCGGTTCGCCGACTGGGACCGCTTCGTCGCCGTGCGCAAGCCGATCATCGCCGCGGTCGCGGGCTACGC
GCTGGGCGGCGGCTGCGAGCTGGCCATGATGTGCGACATCATCCTGGCGGCCGACACGGCGCAGTTCGGCCAGCCCGAGA
TCAAGCTTGGGGTGATGCCGGGGATCGGCGGCAGCCAGCGGCTGACCCGCTTCGTCGGCAAGGCCAAGGCCATGGAGATG
TGCCTGACCGGCCGGATGATGGACGCCGCCGAGGCGGAGCGCGCCGGCCTCGTGTCGCGGGTGATCGCGGCCGACCAGCT
CCTCGACGAGGCGCTGAAGGCGGCCGAGACCATCGCGTCGATGTCGCTGCCGATCGCCATGATGACCAAGGAGGCGGTCA
ACCGGTCCTACGAGACCACGCTGACCGAGGGGATCCGGTTCGAGCGGCGAGTCTTCCACGCGATGTTCGCCACCAAGGAC
CAGAAGGAGGGCATGGCCGCCTTCGTGGAGAAGCGGCCGCCCCGGTTCGAGAACACCTGA

Upstream 100 bases:

>100_bases
CCGAGGCCGCGCAGATCTACGGTCTGTTCGAGGGCGCGGGCCACGGGGGAGAGGACTTTTCCGCGATCATCCGTATGCTT
CGCGGGCAGGGAGGCGAGTC

Downstream 100 bases:

>100_bases
ACGCGGCCCGTCCCGGCGGGAGGGGCGGGGAGCGAGACCATAAGACAGGGAGGGGAACGACCATGCCGAGCTTCCGGGAG
GCCCGCGACCTTCTGCTGGC

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSEPYETILTETRGRVLLITLNRPKALNALNAQLTREVIRAATEADADPGIGCIVVTGSAKAFAAGADIKEMQHATYAEM
YAGDRFADWDRFVAVRKPIIAAVAGYALGGGCELAMMCDIILAADTAQFGQPEIKLGVMPGIGGSQRLTRFVGKAKAMEM
CLTGRMMDAAEAERAGLVSRVIAADQLLDEALKAAETIASMSLPIAMMTKEAVNRSYETTLTEGIRFERRVFHAMFATKD
QKEGMAAFVEKRPPRFENT

Sequences:

>Translated_259_residues
MSEPYETILTETRGRVLLITLNRPKALNALNAQLTREVIRAATEADADPGIGCIVVTGSAKAFAAGADIKEMQHATYAEM
YAGDRFADWDRFVAVRKPIIAAVAGYALGGGCELAMMCDIILAADTAQFGQPEIKLGVMPGIGGSQRLTRFVGKAKAMEM
CLTGRMMDAAEAERAGLVSRVIAADQLLDEALKAAETIASMSLPIAMMTKEAVNRSYETTLTEGIRFERRVFHAMFATKD
QKEGMAAFVEKRPPRFENT
>Mature_258_residues
SEPYETILTETRGRVLLITLNRPKALNALNAQLTREVIRAATEADADPGIGCIVVTGSAKAFAAGADIKEMQHATYAEMY
AGDRFADWDRFVAVRKPIIAAVAGYALGGGCELAMMCDIILAADTAQFGQPEIKLGVMPGIGGSQRLTRFVGKAKAMEMC
LTGRMMDAAEAERAGLVSRVIAADQLLDEALKAAETIASMSLPIAMMTKEAVNRSYETTLTEGIRFERRVFHAMFATKDQ
KEGMAAFVEKRPPRFENT

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=257, Percent_Identity=54.0856031128405, Blast_Score=279, Evalue=2e-75,
Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=35.8267716535433, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI31542718, Length=265, Percent_Identity=28.6792452830189, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI70995211, Length=269, Percent_Identity=31.5985130111524, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI68989263, Length=186, Percent_Identity=33.8709677419355, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI150378541, Length=243, Percent_Identity=31.6872427983539, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI37594471, Length=209, Percent_Identity=31.1004784688995, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI37594469, Length=209, Percent_Identity=31.1004784688995, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI20127408, Length=181, Percent_Identity=33.7016574585635, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI25453481, Length=258, Percent_Identity=25.5813953488372, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI51538573, Length=258, Percent_Identity=25.5813953488372, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI4757968, Length=257, Percent_Identity=24.5136186770428, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI49355787, Length=257, Percent_Identity=24.5136186770428, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI45643119, Length=246, Percent_Identity=26.8292682926829, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI260275230, Length=246, Percent_Identity=26.8292682926829, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI260274832, Length=224, Percent_Identity=27.2321428571429, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI4757966, Length=208, Percent_Identity=26.9230769230769, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI51538581, Length=208, Percent_Identity=26.9230769230769, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI221136756, Length=235, Percent_Identity=26.3829787234043, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI221136753, Length=235, Percent_Identity=26.3829787234043, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI221307494, Length=235, Percent_Identity=26.3829787234043, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI157694516, Length=240, Percent_Identity=23.3333333333333, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI116235487, Length=209, Percent_Identity=26.7942583732057, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI213417737, Length=231, Percent_Identity=23.3766233766234, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI261878539, Length=97, Percent_Identity=37.1134020618557, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=45.7364341085271, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI221142681, Length=259, Percent_Identity=36.2934362934363, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=34.469696969697, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=31.1284046692607, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87082183, Length=258, Percent_Identity=30.2325581395349, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1788682, Length=201, Percent_Identity=34.3283582089552, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790281, Length=234, Percent_Identity=29.0598290598291, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=246, Percent_Identity=56.5040650406504, Blast_Score=283, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI25145438, Length=245, Percent_Identity=48.5714285714286, Blast_Score=249, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17540714, Length=224, Percent_Identity=33.9285714285714, Blast_Score=120, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17560910, Length=253, Percent_Identity=30.4347826086957, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17540306, Length=243, Percent_Identity=31.2757201646091, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17549921, Length=236, Percent_Identity=31.7796610169492, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=272, Percent_Identity=29.7794117647059, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17534483, Length=260, Percent_Identity=27.6923076923077, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17558304, Length=185, Percent_Identity=31.3513513513514, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=192, Percent_Identity=30.7291666666667, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI25144160, Length=192, Percent_Identity=30.7291666666667, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI25144276, Length=215, Percent_Identity=31.6279069767442, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17508953, Length=215, Percent_Identity=31.6279069767442, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17508951, Length=215, Percent_Identity=31.6279069767442, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=26.0869565217391, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25141363, Length=204, Percent_Identity=26.9607843137255, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=183, Percent_Identity=27.3224043715847, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20129971, Length=260, Percent_Identity=60, Blast_Score=309, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24653477, Length=260, Percent_Identity=60, Blast_Score=309, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24653139, Length=259, Percent_Identity=35.1351351351351, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24650670, Length=214, Percent_Identity=32.7102803738318, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922422, Length=259, Percent_Identity=25.8687258687259, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=31.0160427807487, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI19920382, Length=230, Percent_Identity=35.2173913043478, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21357171, Length=266, Percent_Identity=27.0676691729323, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28571730, Length=175, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI28571729, Length=175, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI19921000, Length=180, Percent_Identity=32.7777777777778, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583077, Length=180, Percent_Identity=32.7777777777778, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583079, Length=180, Percent_Identity=32.7777777777778, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28062; Mature: 27931

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPYETILTETRGRVLLITLNRPKALNALNAQLTREVIRAATEADADPGIGCIVVTGSA
CCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
KAFAAGADIKEMQHATYAEMYAGDRFADWDRFVAVRKPIIAAVAGYALGGGCELAMMCDI
CHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ILAADTAQFGQPEIKLGVMPGIGGSQRLTRFVGKAKAMEMCLTGRMMDAAEAERAGLVSR
HHHCCCHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VIAADQLLDEALKAAETIASMSLPIAMMTKEAVNRSYETTLTEGIRFERRVFHAMFATKD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
QKEGMAAFVEKRPPRFENT
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SEPYETILTETRGRVLLITLNRPKALNALNAQLTREVIRAATEADADPGIGCIVVTGSA
CCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
KAFAAGADIKEMQHATYAEMYAGDRFADWDRFVAVRKPIIAAVAGYALGGGCELAMMCDI
CHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ILAADTAQFGQPEIKLGVMPGIGGSQRLTRFVGKAKAMEMCLTGRMMDAAEAERAGLVSR
HHHCCCHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VIAADQLLDEALKAAETIASMSLPIAMMTKEAVNRSYETTLTEGIRFERRVFHAMFATKD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
QKEGMAAFVEKRPPRFENT
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]