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The map label for this gene is grpE [H]

Identifier: 170749944

GI number: 170749944

Start: 3765350

End: 3765964

Strand: Direct

Name: grpE [H]

Synonym: Mrad2831_3544

Alternate gene names: 170749944

Gene position: 3765350-3765964 (Clockwise)

Preceding gene: 170749943

Following gene: 170749949

Centisome position: 61.95

GC content: 73.5

Gene sequence:

>615_bases
ATGCCCCAGGATGACCAGCGCCAGACCACGGTCGAAGCCGGTGCCGGCGCGGCCCAGGATCCCGCGCCCGCGGGTCAGGG
CAGCGAGTCGGCCACCGTCGATCCGGTCGCGGAGGCCCTGGCCCTGCTGACCGCCGAGCGCGACGAGCTCAAGGACCGGA
CGCTGCGCACCCTCGCCGAGATGGAGAACCTGCGCCGCCGGACGGAGCGCGAGGTCGCCGACGCGCGCGCCTACGCGGTG
ACCAACTTCGCCCGGGACGTGCTCAACGTCGCCGACAACATCCGCCGCGCCCTCGATAGCGTCCCGGCCGACGCGAAGGC
CACGGCCGACGGCGCCCTGAAGGGGCTGATCGACGGGATCGAGCTCACCGAGCGCGACCTCGCCAAGACCCTCGAGCGCC
ACGGCGTCAAGATCGTCGAGCCGCAGGGCCAGAAGTTCGACCCGAACCGCCATCAGGCGATGTTCGAGGTGCCGAACGCG
GAGGTTCCGGCCGGCACCGTCGTGCAGGTCGTGCAGGCGGGCTACGTCATCGGCGAGCGCGTGCTGCGCCCGGCCCTGGT
CGGCGTCGCCAAGGGTGGCCCGAAGGCGGCCGCCAACCCGGCCGACGCCGCCTGA

Upstream 100 bases:

>100_bases
TGCGGGTTGATTTCGCGGCCCCGTGGCCAGATATCAGCGCCGATTCCATCATCCCTCACCGATTCCCGCCCACGACTGAA
ACGTGATGACGAGCAACGAC

Downstream 100 bases:

>100_bases
GCGGTCACCGCGGCGCGCCCTACTGACAGGGCAGCGCCGCGGCGAAATCCGAGATGAACTGCGCGGTCTGGCGGGTCTGG
GCCGGCGCGGCCGGATCCCG

Product: ribulose-phosphate 3-epimerase

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MPQDDQRQTTVEAGAGAAQDPAPAGQGSESATVDPVAEALALLTAERDELKDRTLRTLAEMENLRRRTEREVADARAYAV
TNFARDVLNVADNIRRALDSVPADAKATADGALKGLIDGIELTERDLAKTLERHGVKIVEPQGQKFDPNRHQAMFEVPNA
EVPAGTVVQVVQAGYVIGERVLRPALVGVAKGGPKAAANPADAA

Sequences:

>Translated_204_residues
MPQDDQRQTTVEAGAGAAQDPAPAGQGSESATVDPVAEALALLTAERDELKDRTLRTLAEMENLRRRTEREVADARAYAV
TNFARDVLNVADNIRRALDSVPADAKATADGALKGLIDGIELTERDLAKTLERHGVKIVEPQGQKFDPNRHQAMFEVPNA
EVPAGTVVQVVQAGYVIGERVLRPALVGVAKGGPKAAANPADAA
>Mature_203_residues
PQDDQRQTTVEAGAGAAQDPAPAGQGSESATVDPVAEALALLTAERDELKDRTLRTLAEMENLRRRTEREVADARAYAVT
NFARDVLNVADNIRRALDSVPADAKATADGALKGLIDGIELTERDLAKTLERHGVKIVEPQGQKFDPNRHQAMFEVPNAE
VPAGTVVQVVQAGYVIGERVLRPALVGVAKGGPKAAANPADAA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=173, Percent_Identity=37.5722543352601, Blast_Score=111, Evalue=4e-25,
Organism=Homo sapiens, GI40255109, Length=154, Percent_Identity=35.0649350649351, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI1788967, Length=164, Percent_Identity=38.4146341463415, Blast_Score=98, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17552458, Length=149, Percent_Identity=40.9395973154362, Blast_Score=123, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6324806, Length=164, Percent_Identity=35.3658536585366, Blast_Score=121, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24653432, Length=162, Percent_Identity=38.8888888888889, Blast_Score=133, Evalue=1e-31,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21606; Mature: 21475

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQDDQRQTTVEAGAGAAQDPAPAGQGSESATVDPVAEALALLTAERDELKDRTLRTLAE
CCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MENLRRRTEREVADARAYAVTNFARDVLNVADNIRRALDSVPADAKATADGALKGLIDGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
ELTERDLAKTLERHGVKIVEPQGQKFDPNRHQAMFEVPNAEVPAGTVVQVVQAGYVIGER
HHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHEEECCCCCCCHHHHHHHHHHHHHHHHH
VLRPALVGVAKGGPKAAANPADAA
HHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PQDDQRQTTVEAGAGAAQDPAPAGQGSESATVDPVAEALALLTAERDELKDRTLRTLAE
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MENLRRRTEREVADARAYAVTNFARDVLNVADNIRRALDSVPADAKATADGALKGLIDGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
ELTERDLAKTLERHGVKIVEPQGQKFDPNRHQAMFEVPNAEVPAGTVVQVVQAGYVIGER
HHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHEEECCCCCCCHHHHHHHHHHHHHHHHH
VLRPALVGVAKGGPKAAANPADAA
HHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA