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The map label for this gene is eda [H]

Identifier: 170749829

GI number: 170749829

Start: 3642906

End: 3643532

Strand: Direct

Name: eda [H]

Synonym: Mrad2831_3429

Alternate gene names: 170749829

Gene position: 3642906-3643532 (Clockwise)

Preceding gene: 170749828

Following gene: 170749832

Centisome position: 59.94

GC content: 74.32

Gene sequence:

>627_bases
ATGAACGATCTGACCAGCATCGACGCGCTGATGCGCTCCGTGCCCGTCATTCCGGTCCTGGTGATCGACGACGCGGCCCA
GGCGCGGCCCATCGCCGAGGCCCTGGTCAAGGGCGGCCTGACCGCCCTGGAGGTCACCCTGCGGACCCCGGCCGCCCTCG
ACGTCATCCGGGAGATGACGCGGGTCGAGGGCGCGGTGGTGGGCGCCGGGACGGTGCTCAACCCGCGCGATCTCGAGAAG
GCGCTGGCGGCGGGGGCCGAGTTCATCGTCTCGCCGGGCCTCACCGAGCCGCTGACCGACGCCGCCAAGGCCAACGGCGT
GCCCTACCTGCCCGGCGTGGCCACGGCGGCCGACATCATGCGCGGCCTCGACCACGGCCTCGACCGGTTCAAGTTCTTCC
CGGCCGAGGCCGCGGGCGGGATCAAGGCGCTGAAGGCGCACGCGGCCGTGTTCGGCGCGGTGCGGTTCTGCCCCACGGGC
GGGATCACCGAGGCGACCGCTCCCGAATGGCTGTCGATCCCGTCCGTGCTCTGCGTCGGCGGCAGCTGGGTGGTGAAGCC
CGGCGCCCCCGACCCGGCGGCGATCGAGCGGGCGGCCCGGGCCGCCGCCGGGTTGCGGACGGCGTAA

Upstream 100 bases:

>100_bases
CTGTTCGCGTTCATGCGCCAGGGGGCCGACACGGCGGAGCGGGGCGCCTCGGCGATGCTCGCCGCCGCCGGCCTCTGAAA
CCGAGAGGGAGTTGCCGACC

Downstream 100 bases:

>100_bases
GCCTCACGACCGAAGCCCTTGCGAGCAGAGCGAAAGGTCCGGGGTCGCGCCAAATCTTCGAGATGTCGCGCTGCCCTGGA
TCACCTCGCCGCGCTCGCGA

Product: 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MNDLTSIDALMRSVPVIPVLVIDDAAQARPIAEALVKGGLTALEVTLRTPAALDVIREMTRVEGAVVGAGTVLNPRDLEK
ALAAGAEFIVSPGLTEPLTDAAKANGVPYLPGVATAADIMRGLDHGLDRFKFFPAEAAGGIKALKAHAAVFGAVRFCPTG
GITEATAPEWLSIPSVLCVGGSWVVKPGAPDPAAIERAARAAAGLRTA

Sequences:

>Translated_208_residues
MNDLTSIDALMRSVPVIPVLVIDDAAQARPIAEALVKGGLTALEVTLRTPAALDVIREMTRVEGAVVGAGTVLNPRDLEK
ALAAGAEFIVSPGLTEPLTDAAKANGVPYLPGVATAADIMRGLDHGLDRFKFFPAEAAGGIKALKAHAAVFGAVRFCPTG
GITEATAPEWLSIPSVLCVGGSWVVKPGAPDPAAIERAARAAAGLRTA
>Mature_208_residues
MNDLTSIDALMRSVPVIPVLVIDDAAQARPIAEALVKGGLTALEVTLRTPAALDVIREMTRVEGAVVGAGTVLNPRDLEK
ALAAGAEFIVSPGLTEPLTDAAKANGVPYLPGVATAADIMRGLDHGLDRFKFFPAEAAGGIKALKAHAAVFGAVRFCPTG
GITEATAPEWLSIPSVLCVGGSWVVKPGAPDPAAIERAARAAAGLRTA

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=177, Percent_Identity=52.5423728813559, Blast_Score=183, Evalue=9e-48,
Organism=Escherichia coli, GI48994954, Length=186, Percent_Identity=31.1827956989247, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 21236; Mature: 21236

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLTSIDALMRSVPVIPVLVIDDAAQARPIAEALVKGGLTALEVTLRTPAALDVIREMT
CCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHH
RVEGAVVGAGTVLNPRDLEKALAAGAEFIVSPGLTEPLTDAAKANGVPYLPGVATAADIM
HHCCCEECCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHCCCCCCCCCHHHHHHHH
RGLDHGLDRFKFFPAEAAGGIKALKAHAAVFGAVRFCPTGGITEATAPEWLSIPSVLCVG
HHHHHHHHHHEECCCHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCHHEEEC
GSWVVKPGAPDPAAIERAARAAAGLRTA
CCEEECCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNDLTSIDALMRSVPVIPVLVIDDAAQARPIAEALVKGGLTALEVTLRTPAALDVIREMT
CCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHH
RVEGAVVGAGTVLNPRDLEKALAAGAEFIVSPGLTEPLTDAAKANGVPYLPGVATAADIM
HHCCCEECCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHCCCCCCCCCHHHHHHHH
RGLDHGLDRFKFFPAEAAGGIKALKAHAAVFGAVRFCPTGGITEATAPEWLSIPSVLCVG
HHHHHHHHHHEECCCHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCHHEEEC
GSWVVKPGAPDPAAIERAARAAAGLRTA
CCEEECCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1809834 [H]