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The map label for this gene is ansB [H]

Identifier: 170749827

GI number: 170749827

Start: 3639534

End: 3640655

Strand: Direct

Name: ansB [H]

Synonym: Mrad2831_3427

Alternate gene names: 170749827

Gene position: 3639534-3640655 (Clockwise)

Preceding gene: 170749825

Following gene: 170749828

Centisome position: 59.88

GC content: 68.63

Gene sequence:

>1122_bases
ATGTTCTCCTGCCGAAGCGGCGCGGCTCTTCCCCTGCGCGGAGCGGTCTTCGCCCTGGCCCTGGCCAGCGGATTCGGGGC
GGCGCCCCTCGTCGCCCGCGAGATGCCCGAGGCCGGCGCAGCCGCCGCAGCGAAGAAGCCGAACGTCGTGATCCTCGCCA
CCGGCGGGACGATCGCCGGCGCCGGCGCCGACGCCGCCAACAGCGCCACCTACACGGCCGCCAAGGTGCCGGTCGACAAG
CTGATCGCGGCCGTGCCGCAGGTGAACACCATCGCCAACGTCCGCGGCGAGCAGGTCTTCCAGATCGCCTCCGAGAGCTT
CACCGACGCCCAGCTCGTCCAGCTCGGCAAGCGCGTGTCCCAGCTCCTGAAGCAGGACGACGTCGACGGCGTCGTCGTCA
CGCACGGGACCGACACGCTGGAGGAGACCGCGTTCTTCCTCGACCTCGTGGTGAAGAGCGACAAGCCGATCGTGGTGGTC
GGCTCGATGCGGCCGGGCACGGCGATCTCGGCCGACGGCGCCCTCAACCTGCTCGACGCCGTCACGGTCGCCGCCAGCAA
GGAGGCGATCGGCCAGGGCGTCACCGTGACGATGAACGACTACATCCAGTGCGGCCGCGACGTGAACAAGCGGACCAACA
TCGTGCCCAGCGCCTTCTACAGCCAGTGGGGGCCGCTCGGCATGGTGGTCGAGGGCAAGACCTACTTCTTCCGCTCGCTG
ACGAAGCGGCACAACATGAGCTCCGAGTTCGACATCGACCAGATCGACAGCCTGCCGCTGGTCGGCATCGTCTACGGCTC
GGGCAACATGATCCCGGGGGTCTACGACGCCGAGCTCCAGGCGGGCGCCAAGGCGATCGTCAACGCCGGCACCGGCAACG
GCTCGGTGCCCGGCTACCTCGTCGACAAGCTGAAGGACATCCGCTCCAAGGGCGCCATCGTGATCCGCTCGTCGCGGGTG
CCGGACGGCTTCGTCCTGCGCAACGCCGAGCAGCCCGACGACAAGTACGACTGGGTGGTCGCCCACGACCTCAACCCCGC
CAAGGCCAAGATCCTGGCGGCCGTGGCGCTCACCAAGACCAGCGACACCAAGGAGCTGCAGCGCATCTTCTGGGAGTATT
GA

Upstream 100 bases:

>100_bases
CGCGTCGGCGCCCTGCATGATCCTTGCCTCGGCCGGAGGCGCGGCTACCATCCCCGCGGCGAGTCCCACTCGCTCTCCCG
TCAACCGTCGAGGATCTCCG

Downstream 100 bases:

>100_bases
GTCCAGGGACGCGCTGCGCCCCTGAGGTTCAACCCCTCGCTGAGGCAGGCCTCGGAGGAACGCTTCGAGACCTGCCGACG
TCGGGCGTCCGCGCAGGAGC

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase/L-GLNase; L-asparagine/L-glutamine amidohydrolase [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MFSCRSGAALPLRGAVFALALASGFGAAPLVAREMPEAGAAAAAKKPNVVILATGGTIAGAGADAANSATYTAAKVPVDK
LIAAVPQVNTIANVRGEQVFQIASESFTDAQLVQLGKRVSQLLKQDDVDGVVVTHGTDTLEETAFFLDLVVKSDKPIVVV
GSMRPGTAISADGALNLLDAVTVAASKEAIGQGVTVTMNDYIQCGRDVNKRTNIVPSAFYSQWGPLGMVVEGKTYFFRSL
TKRHNMSSEFDIDQIDSLPLVGIVYGSGNMIPGVYDAELQAGAKAIVNAGTGNGSVPGYLVDKLKDIRSKGAIVIRSSRV
PDGFVLRNAEQPDDKYDWVVAHDLNPAKAKILAAVALTKTSDTKELQRIFWEY

Sequences:

>Translated_373_residues
MFSCRSGAALPLRGAVFALALASGFGAAPLVAREMPEAGAAAAAKKPNVVILATGGTIAGAGADAANSATYTAAKVPVDK
LIAAVPQVNTIANVRGEQVFQIASESFTDAQLVQLGKRVSQLLKQDDVDGVVVTHGTDTLEETAFFLDLVVKSDKPIVVV
GSMRPGTAISADGALNLLDAVTVAASKEAIGQGVTVTMNDYIQCGRDVNKRTNIVPSAFYSQWGPLGMVVEGKTYFFRSL
TKRHNMSSEFDIDQIDSLPLVGIVYGSGNMIPGVYDAELQAGAKAIVNAGTGNGSVPGYLVDKLKDIRSKGAIVIRSSRV
PDGFVLRNAEQPDDKYDWVVAHDLNPAKAKILAAVALTKTSDTKELQRIFWEY
>Mature_373_residues
MFSCRSGAALPLRGAVFALALASGFGAAPLVAREMPEAGAAAAAKKPNVVILATGGTIAGAGADAANSATYTAAKVPVDK
LIAAVPQVNTIANVRGEQVFQIASESFTDAQLVQLGKRVSQLLKQDDVDGVVVTHGTDTLEETAFFLDLVVKSDKPIVVV
GSMRPGTAISADGALNLLDAVTVAASKEAIGQGVTVTMNDYIQCGRDVNKRTNIVPSAFYSQWGPLGMVVEGKTYFFRSL
TKRHNMSSEFDIDQIDSLPLVGIVYGSGNMIPGVYDAELQAGAKAIVNAGTGNGSVPGYLVDKLKDIRSKGAIVIRSSRV
PDGFVLRNAEQPDDKYDWVVAHDLNPAKAKILAAVALTKTSDTKELQRIFWEY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=350, Percent_Identity=45.4285714285714, Blast_Score=264, Evalue=6e-72,
Organism=Saccharomyces cerevisiae, GI6323189, Length=367, Percent_Identity=35.149863760218, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6323187, Length=367, Percent_Identity=35.149863760218, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6323186, Length=367, Percent_Identity=35.149863760218, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6323184, Length=367, Percent_Identity=35.149863760218, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6320527, Length=331, Percent_Identity=31.7220543806647, Blast_Score=152, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.38 [H]

Molecular weight: Translated: 39273; Mature: 39273

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSCRSGAALPLRGAVFALALASGFGAAPLVAREMPEAGAAAAAKKPNVVILATGGTIAG
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHCCCCCEEEEECCCEEEC
AGADAANSATYTAAKVPVDKLIAAVPQVNTIANVRGEQVFQIASESFTDAQLVQLGKRVS
CCCCCCCCCEEEEECCCHHHHHHHCCCCHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHH
QLLKQDDVDGVVVTHGTDTLEETAFFLDLVVKSDKPIVVVGSMRPGTAISADGALNLLDA
HHHHHCCCCEEEEECCCCHHHHHHHHHHHEEECCCCEEEEECCCCCCEECCCCCHHHHHH
VTVAASKEAIGQGVTVTMNDYIQCGRDVNKRTNIVPSAFYSQWGPLGMVVEGKTYFFRSL
HHHHHCHHHHCCCEEEEEHHHHHCCCCCCHHCCCCCHHHHHCCCCEEEEECCCHHHHHHH
TKRHNMSSEFDIDQIDSLPLVGIVYGSGNMIPGVYDAELQAGAKAIVNAGTGNGSVPGYL
HHHCCCCCCCCHHHHCCCCEEEEEECCCCCCCCEECHHHHCCCCEEEECCCCCCCCCHHH
VDKLKDIRSKGAIVIRSSRVPDGFVLRNAEQPDDKYDWVVAHDLNPAKAKILAAVALTKT
HHHHHHHHCCCEEEEECCCCCCCEEECCCCCCCCCCCEEEEECCCHHHHHHEEHEEEECC
SDTKELQRIFWEY
CCHHHHHHHHHCC
>Mature Secondary Structure
MFSCRSGAALPLRGAVFALALASGFGAAPLVAREMPEAGAAAAAKKPNVVILATGGTIAG
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHCCCCCEEEEECCCEEEC
AGADAANSATYTAAKVPVDKLIAAVPQVNTIANVRGEQVFQIASESFTDAQLVQLGKRVS
CCCCCCCCCEEEEECCCHHHHHHHCCCCHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHH
QLLKQDDVDGVVVTHGTDTLEETAFFLDLVVKSDKPIVVVGSMRPGTAISADGALNLLDA
HHHHHCCCCEEEEECCCCHHHHHHHHHHHEEECCCCEEEEECCCCCCEECCCCCHHHHHH
VTVAASKEAIGQGVTVTMNDYIQCGRDVNKRTNIVPSAFYSQWGPLGMVVEGKTYFFRSL
HHHHHCHHHHCCCEEEEEHHHHHCCCCCCHHCCCCCHHHHHCCCCEEEEECCCHHHHHHH
TKRHNMSSEFDIDQIDSLPLVGIVYGSGNMIPGVYDAELQAGAKAIVNAGTGNGSVPGYL
HHHCCCCCCCCHHHHCCCCEEEEEECCCCCCCCEECHHHHCCCCEEEECCCCCCCCCHHH
VDKLKDIRSKGAIVIRSSRVPDGFVLRNAEQPDDKYDWVVAHDLNPAKAKILAAVALTKT
HHHHHHHHCCCEEEEECCCCCCCEEECCCCCCCCCCCEEEEECCCHHHHHHEEHEEEECC
SDTKELQRIFWEY
CCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]