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The map label for this gene is mdlB [H]

Identifier: 170749811

GI number: 170749811

Start: 3620831

End: 3622138

Strand: Direct

Name: mdlB [H]

Synonym: Mrad2831_3410

Alternate gene names: 170749811

Gene position: 3620831-3622138 (Clockwise)

Preceding gene: 170749807

Following gene: 170749812

Centisome position: 59.57

GC content: 72.55

Gene sequence:

>1308_bases
ATGGCGACGCTCGACCGGCCGGCCTCCGGACTGGGAGCCCCCGAGCGCGCGGCGCCGACCGTCGGCGGCGCCGACGCGAC
TGCGGCGGGCATCGCCGCCCCGCAGGCCATCGTCACCAGCCTGAAGCGGAGCGACGCGGTCACGCCCTACCGGTTCCGCG
ACCTGCTGGCGCTGGACGACTTCGAGCGGCACGCGCGCAAGCTGCTGCCGCCGATGATCTTCCAGTACGTCTCCGGCGGG
GTCGAGACCGGCTCGGCCCTGGCGCAGAGCCGCGGCGCCTACGCCGACTACGCCCTCGTGCCCCGCCTGATGCGGGACAC
CTCGGCGCGGGACACCGCCACCACGCTGTTCGGCCAGACCTACGCGGCGCCCTTCGGCGTCGGGCCGCTGGGCGGGGCCG
CCTTCATCGCCTACCGGGGCGACCTCGTGCTCGCCGAGGCGGCCCGGCGGATGAACCTGCCGATGTGCCTCAGCGCCTCC
TCGCTGATCAGGCTGGAGGACGTCCACGCCCAGAACCCGCAGGCTTGGTTCCAGGGCTACCTGCCGGGCGACCAGAACCG
GATCGACCGCCTCCTCGACCGGGTCGAGGCCACCGGCTACCGGACCTTCGTGGTCACGGCCGACACCCCGACCCTCGGCA
ACCGCGAGCACAACATCCGCAGCGGCTTCTCCATGCCGATCAAGGTCACGCCGAAGGTGGCGTGGCAGAGCGTCACCCAC
CCGCGCTGGCTGCTCGGAACGGTCGCCCGGACCTTCCTCAAGCACGGGGCCCCGCACTTCGAGAACACGGAGGCCGAGCG
CGGGCCGCCGATGATGTCCCAGGGCGCGGTGCGCAACACCATCGCCCGCGACCAGCTCTCGTGGAAGAACCTGGAAGCGA
TCCGCAAGCGCTGGTCCGGCAACCTGCTTGTGAAGGGCCTGCTGGCGCCGGAGGATGTCGACATCGCGCGGGCGTGCGGC
GCCGACGGCGTGATCCTGTCGACCCACGGCGGCCGCCAGCTCGACTACGCCGTCGCCCCCCTCGACGTGCTGCCGGAGAT
CGCCGCCCGGAAGGGCGGGCTGAAGATCATCGTCGACAGCGGCGTCCGCCGGGGCACCGACGTGATGAAGGCGCTGGCGC
TGGGCGCCGATTTCGTCCTGCTCGGCCGGCCCTTCATGTTCGCCGCGGCGCTCGGCGGCGTCCCGGGCGTCGAGCACGCG
ATGCGGATCCTCAAGGAGGAGCTCAACCGCGACATGGCGCTGATCGGGGTGAACCGCCTGTCCGAACTCAATCCCGATTT
CCTGCGCCGCGCCCCGCGGCGGGCCTGA

Upstream 100 bases:

>100_bases
CTGAGCCGGACCGGCAGAATATTGACACCTCGCTCGGCCGCTGGCTATCTGAAAGCGCTTTCAAGAGGCCGCGCGATCCG
GCGACCCGCAGGAGGACCCG

Downstream 100 bases:

>100_bases
GCCAATGGCGGGAGGATCGACCATGATCGTCTTCCCGAACACGCGCAGCGCGCCGGTCCCCGCGAGGCGGACCCCCTCCC
GGGGCGCCGGAGCGGCCGCT

Product: L-lactate dehydrogenase (cytochrome)

Products: NA

Alternate protein names: L(+)-mandelate dehydrogenase; MDH [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MATLDRPASGLGAPERAAPTVGGADATAAGIAAPQAIVTSLKRSDAVTPYRFRDLLALDDFERHARKLLPPMIFQYVSGG
VETGSALAQSRGAYADYALVPRLMRDTSARDTATTLFGQTYAAPFGVGPLGGAAFIAYRGDLVLAEAARRMNLPMCLSAS
SLIRLEDVHAQNPQAWFQGYLPGDQNRIDRLLDRVEATGYRTFVVTADTPTLGNREHNIRSGFSMPIKVTPKVAWQSVTH
PRWLLGTVARTFLKHGAPHFENTEAERGPPMMSQGAVRNTIARDQLSWKNLEAIRKRWSGNLLVKGLLAPEDVDIARACG
ADGVILSTHGGRQLDYAVAPLDVLPEIAARKGGLKIIVDSGVRRGTDVMKALALGADFVLLGRPFMFAAALGGVPGVEHA
MRILKEELNRDMALIGVNRLSELNPDFLRRAPRRA

Sequences:

>Translated_435_residues
MATLDRPASGLGAPERAAPTVGGADATAAGIAAPQAIVTSLKRSDAVTPYRFRDLLALDDFERHARKLLPPMIFQYVSGG
VETGSALAQSRGAYADYALVPRLMRDTSARDTATTLFGQTYAAPFGVGPLGGAAFIAYRGDLVLAEAARRMNLPMCLSAS
SLIRLEDVHAQNPQAWFQGYLPGDQNRIDRLLDRVEATGYRTFVVTADTPTLGNREHNIRSGFSMPIKVTPKVAWQSVTH
PRWLLGTVARTFLKHGAPHFENTEAERGPPMMSQGAVRNTIARDQLSWKNLEAIRKRWSGNLLVKGLLAPEDVDIARACG
ADGVILSTHGGRQLDYAVAPLDVLPEIAARKGGLKIIVDSGVRRGTDVMKALALGADFVLLGRPFMFAAALGGVPGVEHA
MRILKEELNRDMALIGVNRLSELNPDFLRRAPRRA
>Mature_434_residues
ATLDRPASGLGAPERAAPTVGGADATAAGIAAPQAIVTSLKRSDAVTPYRFRDLLALDDFERHARKLLPPMIFQYVSGGV
ETGSALAQSRGAYADYALVPRLMRDTSARDTATTLFGQTYAAPFGVGPLGGAAFIAYRGDLVLAEAARRMNLPMCLSASS
LIRLEDVHAQNPQAWFQGYLPGDQNRIDRLLDRVEATGYRTFVVTADTPTLGNREHNIRSGFSMPIKVTPKVAWQSVTHP
RWLLGTVARTFLKHGAPHFENTEAERGPPMMSQGAVRNTIARDQLSWKNLEAIRKRWSGNLLVKGLLAPEDVDIARACGA
DGVILSTHGGRQLDYAVAPLDVLPEIAARKGGLKIIVDSGVRRGTDVMKALALGADFVLLGRPFMFAAALGGVPGVEHAM
RILKEELNRDMALIGVNRLSELNPDFLRRAPRRA

Specific function: Reduction of (S)-mandelate to benzoylformate [H]

COG id: COG1304

COG function: function code C; L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases

Gene ontology:

Cell location: Membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FMN hydroxy acid dehydrogenase domain [H]

Homologues:

Organism=Homo sapiens, GI11068137, Length=381, Percent_Identity=32.5459317585302, Blast_Score=205, Evalue=7e-53,
Organism=Homo sapiens, GI54234014, Length=378, Percent_Identity=31.7460317460317, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI7705393, Length=378, Percent_Identity=31.7460317460317, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI1790033, Length=378, Percent_Identity=36.7724867724868, Blast_Score=219, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI193208036, Length=382, Percent_Identity=29.5811518324607, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI32566217, Length=297, Percent_Identity=27.9461279461279, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323587, Length=392, Percent_Identity=31.8877551020408, Blast_Score=182, Evalue=1e-46,
Organism=Drosophila melanogaster, GI281363140, Length=377, Percent_Identity=29.1777188328912, Blast_Score=161, Evalue=8e-40,
Organism=Drosophila melanogaster, GI78707190, Length=377, Percent_Identity=29.1777188328912, Blast_Score=161, Evalue=8e-40,
Organism=Drosophila melanogaster, GI78707188, Length=376, Percent_Identity=29.2553191489362, Blast_Score=161, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR012133
- InterPro:   IPR000262
- InterPro:   IPR008259 [H]

Pfam domain/function: PF01070 FMN_dh [H]

EC number: =1.1.99.31 [H]

Molecular weight: Translated: 47020; Mature: 46888

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLDRPASGLGAPERAAPTVGGADATAAGIAAPQAIVTSLKRSDAVTPYRFRDLLALDD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
FERHARKLLPPMIFQYVSGGVETGSALAQSRGAYADYALVPRLMRDTSARDTATTLFGQT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
YAAPFGVGPLGGAAFIAYRGDLVLAEAARRMNLPMCLSASSLIRLEDVHAQNPQAWFQGY
HCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEEECCCHHEEEHHHCCCCCHHHHHCC
LPGDQNRIDRLLDRVEATGYRTFVVTADTPTLGNREHNIRSGFSMPIKVTPKVAWQSVTH
CCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCHHCCCCCCEEECCCHHHHCCCC
PRWLLGTVARTFLKHGAPHFENTEAERGPPMMSQGAVRNTIARDQLSWKNLEAIRKRWSG
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHCCHHHHHHHHHHCCC
NLLVKGLLAPEDVDIARACGADGVILSTHGGRQLDYAVAPLDVLPEIAARKGGLKIIVDS
CEEEEECCCCCCCHHHHHCCCCCEEEECCCCCEEEEEECCHHHHHHHHHCCCCEEEEECC
GVRRGTDVMKALALGADFVLLGRPFMFAAALGGVPGVEHAMRILKEELNRDMALIGVNRL
CCCCHHHHHHHHHHCCCEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEHHHH
SELNPDFLRRAPRRA
HHCCHHHHHCCCCCC
>Mature Secondary Structure 
ATLDRPASGLGAPERAAPTVGGADATAAGIAAPQAIVTSLKRSDAVTPYRFRDLLALDD
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
FERHARKLLPPMIFQYVSGGVETGSALAQSRGAYADYALVPRLMRDTSARDTATTLFGQT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
YAAPFGVGPLGGAAFIAYRGDLVLAEAARRMNLPMCLSASSLIRLEDVHAQNPQAWFQGY
HCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEEECCCHHEEEHHHCCCCCHHHHHCC
LPGDQNRIDRLLDRVEATGYRTFVVTADTPTLGNREHNIRSGFSMPIKVTPKVAWQSVTH
CCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCHHCCCCCCEEECCCHHHHCCCC
PRWLLGTVARTFLKHGAPHFENTEAERGPPMMSQGAVRNTIARDQLSWKNLEAIRKRWSG
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHCCHHHHHHHHHHCCC
NLLVKGLLAPEDVDIARACGADGVILSTHGGRQLDYAVAPLDVLPEIAARKGGLKIIVDS
CEEEEECCCCCCCHHHHHCCCCCEEEECCCCCEEEEEECCHHHHHHHHHCCCCEEEEECC
GVRRGTDVMKALALGADFVLLGRPFMFAAALGGVPGVEHAMRILKEELNRDMALIGVNRL
CCCCHHHHHHHHHHCCCEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEHHHH
SELNPDFLRRAPRRA
HHCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2271624 [H]