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The map label for this gene is mltB [H]

Identifier: 170749757

GI number: 170749757

Start: 3563164

End: 3564456

Strand: Reverse

Name: mltB [H]

Synonym: Mrad2831_3356

Alternate gene names: 170749757

Gene position: 3564456-3563164 (Counterclockwise)

Preceding gene: 170749761

Following gene: 170749748

Centisome position: 58.65

GC content: 75.87

Gene sequence:

>1293_bases
ATGGACGATCGCGCCGCGCCGAAGCGGCCCGTGACCCGGAGCGGCGCAACGCGCGCGGTCCTGGCGACGGGTGCCGCACT
CGTCTTCGGCACGGTCGTGCCGGCGCGCGCCGCCGGGCCCGACCCCACCCCGCCGGTCGCACCGCAGTCCTTCGCGGCCT
GCCGGGCCGACCTGATCGCCTGGGTCACGGCCCGCGCCGTGCCGCAGGGCCTCGCCGAGGCGCAGCTCGCCCACCTCACG
CCCGACCCCGACGTGATCGCCGCGACCCAGAGCCAGGGCGAGTTCGTCCGGCCGATCTGGGACTACATCGAGGCCAGCGT
CACGCCGGCCCGCGTCGAGTCCGGTCAGCGCAAGCTCGCCAAGCACGCCGACACGCTCGCGGCCATCGAGGCCAAGTACG
GGGTCGACCGCCACATCCTGGTGGCGTTCTGGGGCGTGGAGTCGAGCTACGGCGCCGTGCTCGACAATGCGGCGGTGGTG
AAGCCGGTCGTCCGCTCGCTCGCGACGCTCGGCTGCAGCGATCCCACCCGCATGACCTACTGGCGCGACGAGCTGGCGGC
GGCGCTCCAGATCCTGGCCTGGAACCAGGCGCCCCTCGACCGGATGCCCGCCGGGCTCACCGGATCCTGGGCCGGCGCCA
TGGGGCACACGCAGTTCATGCCCAGCGTCTACCAGCGCGAGGCGGTCGACTTCGACGGGGACGGCCGGCGCGACATCTGG
ACCTCGGTGCCGGACGCGCTCGCGTCAACGGCGAACTACCTGCGCGGCCACGGCTGGAGACCCGGCGAGGGCTGGGGCGC
CGAGGCGGTGCTGCCCGAGACCTTCGACGCGGCCCTGGCCGACGAGACGACGGTCCGCACCGTCGCGGCGTGGCGGGGGC
TCGGCGTGAGGCCCGCCCGCGACCGCGCCCTGCCGGACACGGCCGAGGCGACCCTGATCCTGCCCGCCGGGATCCGCGGC
CCCGCCTTCCTGCTGCAGCCGAACTTCGCGGTGATCCTCCGCTACAACACGGCCCTGGCCTACGCGCTGACCGTCGCGCT
CCTGTCGGAGCGCCTGCGCGGCGACCCGGGCCTGGTGCACGACTGGCCGCGGGGCGACCGGCCCCTCACCGCCGACGAGC
GGCGCGACCTGCAGACCCGCCTGACGGAGCGCGGCTTCGCCACCGGCGGGGTCGACGGCAAGATCGGCCCGAAGACGCGG
GCGGCGCTGCGCGCCTTCCAGGGGTCGGTCGGCCTGCCGCCGGACGGCTACGCCGACGCCGCGCTGCTGGACCGGGTCCG
GGCCGCGCCCTAA

Upstream 100 bases:

>100_bases
GGCGCTGCCGGGGCCGCGGCGAGCCCCGGGCCGCGTCTCGAAGGTGTCGCTGCCGGCCCGCTATGCTACGCCGCCCTCGT
CTGGATCACCGGAGATGCCC

Downstream 100 bases:

>100_bases
CGGGCGCTCACGCCAGGAAGGCGGGCTCGTCCCGGGCGAGCACCCGCTTGACGCTCTCCAGGTGCAGCCGCGCGCTCTCG
GCGCGGCCGGCCGCCTCCTG

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MDDRAAPKRPVTRSGATRAVLATGAALVFGTVVPARAAGPDPTPPVAPQSFAACRADLIAWVTARAVPQGLAEAQLAHLT
PDPDVIAATQSQGEFVRPIWDYIEASVTPARVESGQRKLAKHADTLAAIEAKYGVDRHILVAFWGVESSYGAVLDNAAVV
KPVVRSLATLGCSDPTRMTYWRDELAAALQILAWNQAPLDRMPAGLTGSWAGAMGHTQFMPSVYQREAVDFDGDGRRDIW
TSVPDALASTANYLRGHGWRPGEGWGAEAVLPETFDAALADETTVRTVAAWRGLGVRPARDRALPDTAEATLILPAGIRG
PAFLLQPNFAVILRYNTALAYALTVALLSERLRGDPGLVHDWPRGDRPLTADERRDLQTRLTERGFATGGVDGKIGPKTR
AALRAFQGSVGLPPDGYADAALLDRVRAAP

Sequences:

>Translated_430_residues
MDDRAAPKRPVTRSGATRAVLATGAALVFGTVVPARAAGPDPTPPVAPQSFAACRADLIAWVTARAVPQGLAEAQLAHLT
PDPDVIAATQSQGEFVRPIWDYIEASVTPARVESGQRKLAKHADTLAAIEAKYGVDRHILVAFWGVESSYGAVLDNAAVV
KPVVRSLATLGCSDPTRMTYWRDELAAALQILAWNQAPLDRMPAGLTGSWAGAMGHTQFMPSVYQREAVDFDGDGRRDIW
TSVPDALASTANYLRGHGWRPGEGWGAEAVLPETFDAALADETTVRTVAAWRGLGVRPARDRALPDTAEATLILPAGIRG
PAFLLQPNFAVILRYNTALAYALTVALLSERLRGDPGLVHDWPRGDRPLTADERRDLQTRLTERGFATGGVDGKIGPKTR
AALRAFQGSVGLPPDGYADAALLDRVRAAP
>Mature_430_residues
MDDRAAPKRPVTRSGATRAVLATGAALVFGTVVPARAAGPDPTPPVAPQSFAACRADLIAWVTARAVPQGLAEAQLAHLT
PDPDVIAATQSQGEFVRPIWDYIEASVTPARVESGQRKLAKHADTLAAIEAKYGVDRHILVAFWGVESSYGAVLDNAAVV
KPVVRSLATLGCSDPTRMTYWRDELAAALQILAWNQAPLDRMPAGLTGSWAGAMGHTQFMPSVYQREAVDFDGDGRRDIW
TSVPDALASTANYLRGHGWRPGEGWGAEAVLPETFDAALADETTVRTVAAWRGLGVRPARDRALPDTAEATLILPAGIRG
PAFLLQPNFAVILRYNTALAYALTVALLSERLRGDPGLVHDWPRGDRPLTADERRDLQTRLTERGFATGGVDGKIGPKTR
AALRAFQGSVGLPPDGYADAALLDRVRAAP

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=255, Percent_Identity=33.3333333333333, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 45881; Mature: 45881

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDRAAPKRPVTRSGATRAVLATGAALVFGTVVPARAAGPDPTPPVAPQSFAACRADLIA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
WVTARAVPQGLAEAQLAHLTPDPDVIAATQSQGEFVRPIWDYIEASVTPARVESGQRKLA
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
KHADTLAAIEAKYGVDRHILVAFWGVESSYGAVLDNAAVVKPVVRSLATLGCSDPTRMTY
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCHHH
WRDELAAALQILAWNQAPLDRMPAGLTGSWAGAMGHTQFMPSVYQREAVDFDGDGRRDIW
HHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHH
TSVPDALASTANYLRGHGWRPGEGWGAEAVLPETFDAALADETTVRTVAAWRGLGVRPAR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCC
DRALPDTAEATLILPAGIRGPAFLLQPNFAVILRYNTALAYALTVALLSERLRGDPGLVH
CCCCCCCCCEEEEEECCCCCCEEEECCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCCCC
DWPRGDRPLTADERRDLQTRLTERGFATGGVDGKIGPKTRAALRAFQGSVGLPPDGYADA
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHH
ALLDRVRAAP
HHHHHHHCCC
>Mature Secondary Structure
MDDRAAPKRPVTRSGATRAVLATGAALVFGTVVPARAAGPDPTPPVAPQSFAACRADLIA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
WVTARAVPQGLAEAQLAHLTPDPDVIAATQSQGEFVRPIWDYIEASVTPARVESGQRKLA
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
KHADTLAAIEAKYGVDRHILVAFWGVESSYGAVLDNAAVVKPVVRSLATLGCSDPTRMTY
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCHHH
WRDELAAALQILAWNQAPLDRMPAGLTGSWAGAMGHTQFMPSVYQREAVDFDGDGRRDIW
HHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHH
TSVPDALASTANYLRGHGWRPGEGWGAEAVLPETFDAALADETTVRTVAAWRGLGVRPAR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCC
DRALPDTAEATLILPAGIRGPAFLLQPNFAVILRYNTALAYALTVALLSERLRGDPGLVH
CCCCCCCCCEEEEEECCCCCCEEEECCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCCCC
DWPRGDRPLTADERRDLQTRLTERGFATGGVDGKIGPKTRAALRAFQGSVGLPPDGYADA
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHH
ALLDRVRAAP
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]