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The map label for this gene is gsiD [H]

Identifier: 170749644

GI number: 170749644

Start: 3433860

End: 3434762

Strand: Reverse

Name: gsiD [H]

Synonym: Mrad2831_3241

Alternate gene names: 170749644

Gene position: 3434762-3433860 (Counterclockwise)

Preceding gene: 170749645

Following gene: 170749643

Centisome position: 56.51

GC content: 70.76

Gene sequence:

>903_bases
GTGACCGACATCCTCGTCCCCGCCGCCCCCACCGTCGCGCCCGTCCCCACCGGCATCCGGACGCCGTGGTCGGAATTCTG
GCGCAAGTTCCGCCGCCAGCGCGTTGCCGTCGTCGCCCTGGGCTTCATCGGCCTGCTGGTCGTGGTCGCCGTGCTGGCGC
CGTGGATCGTGCCCTACGACCCGGTCAACGACTTCGACTACGACCGGATCAACGAGGGCCCCTCGCTCGCGCACTGGCTC
GGCGTCGATCCCCTCGGGCGCGACATCCTGAGCCGCATCGTCGAGGGCGCCCGGATCTCCCTCGCGACCGGCTTCCTGTC
GGTCGCCGTCGGCGGCCTCGTCGGGACCGTGCTCGGTCTCCTGGCGGGCTATTACGGCGGCTGGTGGGACCGGATCGTCA
TGCGCATGTCCGACGTGCTGTTCGCCTTCCCGGGCATCCTCCTGGCGCTCGGCGTCGTCGCCATCCTGGGCTCCGGGCTC
GTCAACGTCGTCGTGGCGGTCTCGGTCTTCAGCGTCCCGGCCTTCGCGCGCCTCGTGCGCGGCACGACCCTGGTCCTGAA
GAACAGCACCTACATCGAGGCCGCGCGGAGCATCGGCGCCCCCGACCGGACGATCCTCCTGCGCCACATCCTGCCGGGCA
CGATCTCGGGCATCGTGGTCTACTTCACCATGCGACTGGGCACCTCGATCATCACGGCCGCGAGCCTGTCGTTCCTCGGC
ATGGGCGCGCAGCCGCCGACGCCCGAGTGGGGCGCCATGCTGAACGAGGCGCGCGCCGACATGGTCTCGGCGCCGCACGT
CGCGCTGTTCCCGAGCCTCGCGATCTTCTTCACGGTGCTGGCCTTCAACCTCCTCGGGGACGGGCTCCGGGACGCCCTCG
ACCCGAAGATCGACCGCCTGTGA

Upstream 100 bases:

>100_bases
CTGGTCCTGCTGTTCTCCCTGGAGTTCATCCTGATCAACCTGGTCGTGGACGTGCTCTACGGCCTCATCAACCCGACCAT
CCGCTACAGGTGAGCCGGCC

Downstream 100 bases:

>100_bases
TGCAGGCCGCTCCCGCGCGCCCAGCGCCCCGCGTCGGCCGCCTGCCGCGGGGCCCGCGCGACGCCATCACCGACGTCCCG
GGCGTGACGGTCGGCCATTG

Product: binding-protein-dependent transport systems inner membrane component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MTDILVPAAPTVAPVPTGIRTPWSEFWRKFRRQRVAVVALGFIGLLVVVAVLAPWIVPYDPVNDFDYDRINEGPSLAHWL
GVDPLGRDILSRIVEGARISLATGFLSVAVGGLVGTVLGLLAGYYGGWWDRIVMRMSDVLFAFPGILLALGVVAILGSGL
VNVVVAVSVFSVPAFARLVRGTTLVLKNSTYIEAARSIGAPDRTILLRHILPGTISGIVVYFTMRLGTSIITAASLSFLG
MGAQPPTPEWGAMLNEARADMVSAPHVALFPSLAIFFTVLAFNLLGDGLRDALDPKIDRL

Sequences:

>Translated_300_residues
MTDILVPAAPTVAPVPTGIRTPWSEFWRKFRRQRVAVVALGFIGLLVVVAVLAPWIVPYDPVNDFDYDRINEGPSLAHWL
GVDPLGRDILSRIVEGARISLATGFLSVAVGGLVGTVLGLLAGYYGGWWDRIVMRMSDVLFAFPGILLALGVVAILGSGL
VNVVVAVSVFSVPAFARLVRGTTLVLKNSTYIEAARSIGAPDRTILLRHILPGTISGIVVYFTMRLGTSIITAASLSFLG
MGAQPPTPEWGAMLNEARADMVSAPHVALFPSLAIFFTVLAFNLLGDGLRDALDPKIDRL
>Mature_299_residues
TDILVPAAPTVAPVPTGIRTPWSEFWRKFRRQRVAVVALGFIGLLVVVAVLAPWIVPYDPVNDFDYDRINEGPSLAHWLG
VDPLGRDILSRIVEGARISLATGFLSVAVGGLVGTVLGLLAGYYGGWWDRIVMRMSDVLFAFPGILLALGVVAILGSGLV
NVVVAVSVFSVPAFARLVRGTTLVLKNSTYIEAARSIGAPDRTILLRHILPGTISGIVVYFTMRLGTSIITAASLSFLGM
GAQPPTPEWGAMLNEARADMVSAPHVALFPSLAIFFTVLAFNLLGDGLRDALDPKIDRL

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1173

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787054, Length=283, Percent_Identity=73.1448763250883, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI1789964, Length=292, Percent_Identity=44.8630136986301, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI1787760, Length=252, Percent_Identity=38.0952380952381, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1787498, Length=282, Percent_Identity=37.5886524822695, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1789889, Length=245, Percent_Identity=37.1428571428571, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI1787549, Length=275, Percent_Identity=34.9090909090909, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788505, Length=225, Percent_Identity=32.8888888888889, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32116; Mature: 31985

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDILVPAAPTVAPVPTGIRTPWSEFWRKFRRQRVAVVALGFIGLLVVVAVLAPWIVPYD
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PVNDFDYDRINEGPSLAHWLGVDPLGRDILSRIVEGARISLATGFLSVAVGGLVGTVLGL
CCCCCCCHHCCCCCCHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LAGYYGGWWDRIVMRMSDVLFAFPGILLALGVVAILGSGLVNVVVAVSVFSVPAFARLVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GTTLVLKNSTYIEAARSIGAPDRTILLRHILPGTISGIVVYFTMRLGTSIITAASLSFLG
CCEEEEECCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
MGAQPPTPEWGAMLNEARADMVSAPHVALFPSLAIFFTVLAFNLLGDGLRDALDPKIDRL
CCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TDILVPAAPTVAPVPTGIRTPWSEFWRKFRRQRVAVVALGFIGLLVVVAVLAPWIVPYD
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC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PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]