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The map label for this gene is bioA [C]

Identifier: 170749620

GI number: 170749620

Start: 3402988

End: 3404337

Strand: Reverse

Name: bioA [C]

Synonym: Mrad2831_3217

Alternate gene names: 170749620

Gene position: 3404337-3402988 (Counterclockwise)

Preceding gene: 170749621

Following gene: 170749619

Centisome position: 56.01

GC content: 69.26

Gene sequence:

>1350_bases
ATGACCTTCATGCCCAACTCCGTCGAGGCGCGCGACATCGCCTACCAGCTCCATCCGCTGGTCGACCTGCGCAACTACGA
CAAGACCGGTGCGCTCGTGATCGAGCGGGGGGAGGGGATCTACGTCTACGACAATGCCGGCCGGCGCTACATCGAGGGGC
TGGCGGGCCTCTGGTCGGTCGCCGTGGGCTTCGGCGAGCGGCGGCTGTCGGACGCCGCCAAGCGGCAGATGGACCAGCTC
TCGTACTACCACATCTTCGCGCACAAGACGCACGGCCCCTCGGTTGACCTCGCCGAGAAGCTGATCAGCCTGGCGCCGGT
CCCGATGTCGAAGGTGCATTTCACCTCCTCCGGATCCGAGGCCAACGACCTCGTCGTCAAGCTGGCGTGGTACCGCTCGA
ACGCGCTCGGCAAGCCGGAGAAGAAGAAGATCATCGGCCGCCTGAAGGGCTATCACGGCGTCACCATCGCGGCTGGCTCG
ATCACCGGCCTTCCGCGCAACCACGAGAGCTTCGACCTGCCGCTCGACCGGATGCGGCACGTCTCCTGCCCCGACTACGC
CGGGTCGGCCCTGCCGGGCGAGAGCGAGGCCGCGTTCTCCCGACGCATGGCGGACGAGCTCGAACAGCTGATCCTGGCCG
AGGGGCCGGACACCGTGGCGGCCTTCTTCGGCGAGCCGGTGATGGCCTCGGGCGGGGTCTACGTCCCGCCGGAGAACTAC
TGGGCGGAGATCCAGGCGGTGCTGCGCCGCTACGACGTGCTGCTGGTGGCCGACGAGGTGATCTGCGGCTTCGGGCGCAC
GGGGGCGATGTTCGGCTGCGAGACCTTCGGCATCGAGCCGGACGTGATGGTGCTCTCGAAGCAGATCTCCTCCTCGTACA
TGCCCCTCTCGGCGATCCTGATGAACGCGAACTTCTACGAGCCGATCGCCGACGAATCCGCCCGGCTCGGCGTGCTCGGC
CACGGCTACACGGCCTCGGGCCATCCCGTCGCCACCGCGGTGGGCCTGGAGAACATCCGGATCATCGAGGAGCGCGACCT
CGTGGGGAATTGCCGGCGCCTGAGCCCGCTCTTCCTCGAGCGGCTGGCGGGGCTCGCCGAGCATCCCCTGGTGAAGACGT
GGCGCGGCGTCGGCCTGATCGGGGCCCTCGAGCTGAAGCCCGCGACGGCACCCGGCGCGAAGCCCGGCGCGGCCGGTGCC
GCGATCCAGGCCGCCGCCCTCGAACAGGGGGTGATCGCCCGCGCCATCGGCGATTCCCTGTGCTTCTGCCCGCCGCTGAT
CATCACCGCGGAGCAGATCGGCGCGATGTTCGACGCCGTCGGCCGTGCCCTCGACACGATCGCCGCGTGA

Upstream 100 bases:

>100_bases
CGCCGGAGGCGCCCGCTTCCGCGCCCTCTGCCTCCGTCTCCCTCGCCTCCGTTTCCCTTGCCCCCGTTCCCCTTCCGGCC
GCGATCAGGACACCCGTCTG

Downstream 100 bases:

>100_bases
GCCCGATGGACCAGATCCGCACGACCCGGACCGATCTCGGCGAGATGGACGCGGTGGAGCTCGGGGCGCTGTTCGCGTCC
GGCCGGGTCTCGCCGGTGGA

Product: aminotransferase

Products: S-Adenosyl-4-methylthio-2-oxobutanoate; 7,8-Diaminononanoate [C]

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MTFMPNSVEARDIAYQLHPLVDLRNYDKTGALVIERGEGIYVYDNAGRRYIEGLAGLWSVAVGFGERRLSDAAKRQMDQL
SYYHIFAHKTHGPSVDLAEKLISLAPVPMSKVHFTSSGSEANDLVVKLAWYRSNALGKPEKKKIIGRLKGYHGVTIAAGS
ITGLPRNHESFDLPLDRMRHVSCPDYAGSALPGESEAAFSRRMADELEQLILAEGPDTVAAFFGEPVMASGGVYVPPENY
WAEIQAVLRRYDVLLVADEVICGFGRTGAMFGCETFGIEPDVMVLSKQISSSYMPLSAILMNANFYEPIADESARLGVLG
HGYTASGHPVATAVGLENIRIIEERDLVGNCRRLSPLFLERLAGLAEHPLVKTWRGVGLIGALELKPATAPGAKPGAAGA
AIQAAALEQGVIARAIGDSLCFCPPLIITAEQIGAMFDAVGRALDTIAA

Sequences:

>Translated_449_residues
MTFMPNSVEARDIAYQLHPLVDLRNYDKTGALVIERGEGIYVYDNAGRRYIEGLAGLWSVAVGFGERRLSDAAKRQMDQL
SYYHIFAHKTHGPSVDLAEKLISLAPVPMSKVHFTSSGSEANDLVVKLAWYRSNALGKPEKKKIIGRLKGYHGVTIAAGS
ITGLPRNHESFDLPLDRMRHVSCPDYAGSALPGESEAAFSRRMADELEQLILAEGPDTVAAFFGEPVMASGGVYVPPENY
WAEIQAVLRRYDVLLVADEVICGFGRTGAMFGCETFGIEPDVMVLSKQISSSYMPLSAILMNANFYEPIADESARLGVLG
HGYTASGHPVATAVGLENIRIIEERDLVGNCRRLSPLFLERLAGLAEHPLVKTWRGVGLIGALELKPATAPGAKPGAAGA
AIQAAALEQGVIARAIGDSLCFCPPLIITAEQIGAMFDAVGRALDTIAA
>Mature_448_residues
TFMPNSVEARDIAYQLHPLVDLRNYDKTGALVIERGEGIYVYDNAGRRYIEGLAGLWSVAVGFGERRLSDAAKRQMDQLS
YYHIFAHKTHGPSVDLAEKLISLAPVPMSKVHFTSSGSEANDLVVKLAWYRSNALGKPEKKKIIGRLKGYHGVTIAAGSI
TGLPRNHESFDLPLDRMRHVSCPDYAGSALPGESEAAFSRRMADELEQLILAEGPDTVAAFFGEPVMASGGVYVPPENYW
AEIQAVLRRYDVLLVADEVICGFGRTGAMFGCETFGIEPDVMVLSKQISSSYMPLSAILMNANFYEPIADESARLGVLGH
GYTASGHPVATAVGLENIRIIEERDLVGNCRRLSPLFLERLAGLAEHPLVKTWRGVGLIGALELKPATAPGAKPGAAGAA
IQAAALEQGVIARAIGDSLCFCPPLIITAEQIGAMFDAVGRALDTIAA

Specific function: Biotin biosynthesis. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=436, Percent_Identity=25.9174311926606, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI13994255, Length=382, Percent_Identity=26.1780104712042, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI37574042, Length=427, Percent_Identity=26.6978922716628, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI226442705, Length=427, Percent_Identity=26.9320843091335, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI226442709, Length=387, Percent_Identity=27.390180878553, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI24119277, Length=423, Percent_Identity=26.241134751773, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI284507298, Length=332, Percent_Identity=25.3012048192771, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI188536080, Length=384, Percent_Identity=23.1770833333333, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI38679950, Length=384, Percent_Identity=23.1770833333333, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI38679946, Length=384, Percent_Identity=23.1770833333333, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786991, Length=415, Percent_Identity=30.8433734939759, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1788044, Length=415, Percent_Identity=27.710843373494, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1789759, Length=395, Percent_Identity=26.8354430379747, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787560, Length=419, Percent_Identity=29.3556085918854, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI145693181, Length=418, Percent_Identity=26.7942583732057, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789016, Length=421, Percent_Identity=25.8907363420428, Blast_Score=112, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI32564660, Length=443, Percent_Identity=27.5395033860045, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25144271, Length=422, Percent_Identity=26.7772511848341, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71992977, Length=431, Percent_Identity=24.5939675174014, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI25144274, Length=232, Percent_Identity=30.1724137931034, Blast_Score=105, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323470, Length=448, Percent_Identity=23.4375, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6324386, Length=415, Percent_Identity=25.7831325301205, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324432, Length=439, Percent_Identity=25.0569476082005, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321456, Length=376, Percent_Identity=25.2659574468085, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI161085790, Length=433, Percent_Identity=29.7921478060046, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI28574759, Length=429, Percent_Identity=29.8368298368298, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI21357415, Length=440, Percent_Identity=25.9090909090909, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21356575, Length=367, Percent_Identity=28.6103542234332, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62 [C]

Molecular weight: Translated: 48313; Mature: 48182

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFMPNSVEARDIAYQLHPLVDLRNYDKTGALVIERGEGIYVYDNAGRRYIEGLAGLWSV
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCHHHHHHHHHHHHH
AVGFGERRLSDAAKRQMDQLSYYHIFAHKTHGPSVDLAEKLISLAPVPMSKVHFTSSGSE
HHHCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCCCC
ANDLVVKLAWYRSNALGKPEKKKIIGRLKGYHGVTIAAGSITGLPRNHESFDLPLDRMRH
CCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCEECCCCCCCCCCCCHHHHHC
VSCPDYAGSALPGESEAAFSRRMADELEQLILAEGPDTVAAFFGEPVMASGGVYVPPENY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEECCCEEECCHHH
WAEIQAVLRRYDVLLVADEVICGFGRTGAMFGCETFGIEPDVMVLSKQISSSYMPLSAIL
HHHHHHHHHHHCEEEEECHHHHCCCCCCCCCCCHHCCCCCCHHHHHHHHHCCCCCHHHHE
MNANFYEPIADESARLGVLGHGYTASGHPVATAVGLENIRIIEERDLVGNCRRLSPLFLE
ECCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCEEEEECCCHHCCHHHCCHHHHH
RLAGLAEHPLVKTWRGVGLIGALELKPATAPGAKPGAAGAAIQAAALEQGVIARAIGDSL
HHHHHHCCCHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCH
CFCPPLIITAEQIGAMFDAVGRALDTIAA
HCCCCCEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TFMPNSVEARDIAYQLHPLVDLRNYDKTGALVIERGEGIYVYDNAGRRYIEGLAGLWSV
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCHHHHHHHHHHHHH
AVGFGERRLSDAAKRQMDQLSYYHIFAHKTHGPSVDLAEKLISLAPVPMSKVHFTSSGSE
HHHCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCCCC
ANDLVVKLAWYRSNALGKPEKKKIIGRLKGYHGVTIAAGSITGLPRNHESFDLPLDRMRH
CCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCEECCCCCCCCCCCCHHHHHC
VSCPDYAGSALPGESEAAFSRRMADELEQLILAEGPDTVAAFFGEPVMASGGVYVPPENY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEECCCEEECCHHH
WAEIQAVLRRYDVLLVADEVICGFGRTGAMFGCETFGIEPDVMVLSKQISSSYMPLSAIL
HHHHHHHHHHHCEEEEECHHHHCCCCCCCCCCCHHCCCCCCHHHHHHHHHCCCCCHHHHE
MNANFYEPIADESARLGVLGHGYTASGHPVATAVGLENIRIIEERDLVGNCRRLSPLFLE
ECCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCEEEEECCCHHCCHHHCCHHHHH
RLAGLAEHPLVKTWRGVGLIGALELKPATAPGAKPGAAGAAIQAAALEQGVIARAIGDSL
HHHHHHCCCHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCH
CFCPPLIITAEQIGAMFDAVGRALDTIAA
HCCCCCEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 8-Amino-7-oxononanoate; S-Adenosyl-L-methionine [C]

Specific reaction: 8-Amino-7-oxononanoate + S-Adenosyl-L-methionine <==> S-Adenosyl-4-methylthio-2-oxobutanoate + 7,8-Diaminononanoate [C]

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]