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The map label for this gene is bepF [H]

Identifier: 170749188

GI number: 170749188

Start: 2920018

End: 2921112

Strand: Reverse

Name: bepF [H]

Synonym: Mrad2831_2781

Alternate gene names: 170749188

Gene position: 2921112-2920018 (Counterclockwise)

Preceding gene: 170749189

Following gene: 170749187

Centisome position: 48.06

GC content: 74.06

Gene sequence:

>1095_bases
ATGAAGACGCGCGCGTATCTCGCACTCGGACTCGTCACGGCGGCGCTTGCCGGCTGCAACGGTCACGGCGAGGAGGGCGG
CGAGGCCGCTGCCGTCCGTCCGGTGCTGACGCAGGTGGTCGCGCCGAAGGACAGCATCACGTTCGGGCCGTTCGCCGGCA
CGATCGAGCCGCGCTACCAGGCGCAGCTCGGCTTCCAGATTCCCGGCCGCATGGTGGCTCGGGACGTCACGGTCGGCGAC
CTCGTCACCCGGGGCCAGCGCCTCGCCGCTCTCGACACCATCGTGACCCGCTTCGACCTGACCCGCGCCGAGGCCGACCT
CGCCGACGCGAAGGCGCAGGCCGAGAATGCCGAGGCCGCGGAGGCGCGGACCCGGCGCCTGATGTCCGGCAACGCCGTTT
CCCAGGCGACCCTCGACCAGGCGGTCGCCAAGCGCGAGACCGCCCGGGCCCGGGTGGATCAGGCGATGGCGAGCCTCCAG
AAGGCCCGCGACCAGATGGGCTACACCGAGCTGAAGGCCGAGTTCGACGGCGTCGTGACCCAGCGCCTCGCCGAGGTCGG
CCAGGTGCTCAGCGCCGGCCAGGGTGTCGTGACCGTGGCGCGGCCGGACGTCCGCGAGGCCGTGGTCGACATTCCCGAAG
CCCATGCCGGGGCCCTGCCGGCGGACGGCGTCTTCACCGTCGCGCTCCAGAGCGCGCCGGAGCTGACGGCGCGCGGGCGC
GTCCGCGAGATCGCGCCGCTCGCCGAGTCGGGGACGCGGTCCAAGCGCATCCGGATCACCCTGGACGATCCCGGCCCGGC
CTTCCGGCTCGGCGCGACCATCAACGTGTCGCTCATGCGCAAGGTCCCGCCCACCGTACGGCTGCCCGCCTCGGCGCTGC
TCGAGGACGGCGCCCGCCACTCGGTCTGGGTCGTGTCGCCCGACGGGAAGTCGGTGTCGCGCCGCGACGTCACCGTGGCC
GCGGCGCGGGACAACGCGGACGACGGCCGCGTCGCGGTCGTCGACGGTCTGAAGGCCGGCGAGCGCGTCGTCGTGGCCGG
CGTCCACGTCCTCAAGGAAGGCCAGGCCGTGCGCCTGACCGACGGCGCCGTCTGA

Upstream 100 bases:

>100_bases
TGAAGCGCGGCGTCGAGCCGGGCGAGCGCGTGGTCGTCGCCGGCATCCAGTTCCTGCGCCCCGGCCAGACCGTCGCGGCC
GCCGAGATCGGAGACCGCCC

Downstream 100 bases:

>100_bases
TCCGGCCGCACGGGGTTTTCGCGTGAAGGACTTCAACCTTTCCGACTGGGCGCTCGAGCACCGCTCGTTCGTCTGGTTCC
TGATGCTGGTCTCGCTGGTC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKTRAYLALGLVTAALAGCNGHGEEGGEAAAVRPVLTQVVAPKDSITFGPFAGTIEPRYQAQLGFQIPGRMVARDVTVGD
LVTRGQRLAALDTIVTRFDLTRAEADLADAKAQAENAEAAEARTRRLMSGNAVSQATLDQAVAKRETARARVDQAMASLQ
KARDQMGYTELKAEFDGVVTQRLAEVGQVLSAGQGVVTVARPDVREAVVDIPEAHAGALPADGVFTVALQSAPELTARGR
VREIAPLAESGTRSKRIRITLDDPGPAFRLGATINVSLMRKVPPTVRLPASALLEDGARHSVWVVSPDGKSVSRRDVTVA
AARDNADDGRVAVVDGLKAGERVVVAGVHVLKEGQAVRLTDGAV

Sequences:

>Translated_364_residues
MKTRAYLALGLVTAALAGCNGHGEEGGEAAAVRPVLTQVVAPKDSITFGPFAGTIEPRYQAQLGFQIPGRMVARDVTVGD
LVTRGQRLAALDTIVTRFDLTRAEADLADAKAQAENAEAAEARTRRLMSGNAVSQATLDQAVAKRETARARVDQAMASLQ
KARDQMGYTELKAEFDGVVTQRLAEVGQVLSAGQGVVTVARPDVREAVVDIPEAHAGALPADGVFTVALQSAPELTARGR
VREIAPLAESGTRSKRIRITLDDPGPAFRLGATINVSLMRKVPPTVRLPASALLEDGARHSVWVVSPDGKSVSRRDVTVA
AARDNADDGRVAVVDGLKAGERVVVAGVHVLKEGQAVRLTDGAV
>Mature_364_residues
MKTRAYLALGLVTAALAGCNGHGEEGGEAAAVRPVLTQVVAPKDSITFGPFAGTIEPRYQAQLGFQIPGRMVARDVTVGD
LVTRGQRLAALDTIVTRFDLTRAEADLADAKAQAENAEAAEARTRRLMSGNAVSQATLDQAVAKRETARARVDQAMASLQ
KARDQMGYTELKAEFDGVVTQRLAEVGQVLSAGQGVVTVARPDVREAVVDIPEAHAGALPADGVFTVALQSAPELTARGR
VREIAPLAESGTRSKRIRITLDDPGPAFRLGATINVSLMRKVPPTVRLPASALLEDGARHSVWVVSPDGKSVSRRDVTVA
AARDNADDGRVAVVDGLKAGERVVVAGVHVLKEGQAVRLTDGAV

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=330, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87082036, Length=327, Percent_Identity=25.6880733944954, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1786787, Length=181, Percent_Identity=27.0718232044199, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 38240; Mature: 38240

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRAYLALGLVTAALAGCNGHGEEGGEAAAVRPVLTQVVAPKDSITFGPFAGTIEPRYQ
CCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHH
AQLGFQIPGRMVARDVTVGDLVTRGQRLAALDTIVTRFDLTRAEADLADAKAQAENAEAA
EEECCCCCCHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
EARTRRLMSGNAVSQATLDQAVAKRETARARVDQAMASLQKARDQMGYTELKAEFDGVVT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
QRLAEVGQVLSAGQGVVTVARPDVREAVVDIPEAHAGALPADGVFTVALQSAPELTARGR
HHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHCCC
VREIAPLAESGTRSKRIRITLDDPGPAFRLGATINVSLMRKVPPTVRLPASALLEDGARH
HHHHHHHHCCCCCCCEEEEEECCCCCCEEECCEEEEEEHHHCCCCCCCCHHHHHCCCCCC
SVWVVSPDGKSVSRRDVTVAAARDNADDGRVAVVDGLKAGERVVVAGVHVLKEGQAVRLT
EEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCEEEEEEHHHHCCCCEEEEC
DGAV
CCCC
>Mature Secondary Structure
MKTRAYLALGLVTAALAGCNGHGEEGGEAAAVRPVLTQVVAPKDSITFGPFAGTIEPRYQ
CCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHH
AQLGFQIPGRMVARDVTVGDLVTRGQRLAALDTIVTRFDLTRAEADLADAKAQAENAEAA
EEECCCCCCHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
EARTRRLMSGNAVSQATLDQAVAKRETARARVDQAMASLQKARDQMGYTELKAEFDGVVT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
QRLAEVGQVLSAGQGVVTVARPDVREAVVDIPEAHAGALPADGVFTVALQSAPELTARGR
HHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHCCC
VREIAPLAESGTRSKRIRITLDDPGPAFRLGATINVSLMRKVPPTVRLPASALLEDGARH
HHHHHHHHCCCCCCCEEEEEECCCCCCEEECCEEEEEEHHHCCCCCCCCHHHHHCCCCCC
SVWVVSPDGKSVSRRDVTVAAARDNADDGRVAVVDGLKAGERVVVAGVHVLKEGQAVRLT
EEEEECCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCEEEEEEHHHHCCCCEEEEC
DGAV
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]