Click here to switch to the map view.

The map label for this gene is gltX [H]

Identifier: 170748809

GI number: 170748809

Start: 2545304

End: 2546146

Strand: Reverse

Name: gltX [H]

Synonym: Mrad2831_2391

Alternate gene names: 170748809

Gene position: 2546146-2545304 (Counterclockwise)

Preceding gene: 170748810

Following gene: 170748807

Centisome position: 41.89

GC content: 75.09

Gene sequence:

>843_bases
GTGCTGCGCTTCGCGCCGAGCCCGAACGGGCGGCTGCACCTCGGCCACGCCTACTCGGCCCTGCTCAACGCGGACCTCGC
CGCCCGGATGGGCGGCGTCTGCCGCCTCCGGATCGAGGACATCGATCCCGCGCGCTCGAAGCCGGACTTCGTCCACGGGA
TCGTCACCGACCTCGCCTGGCTCGGGCTGACCTATCCCGAGCCGGTGCGGCATCAGTCCCGGCACATGGCGGACTATCGG
GCCGCCCTCGACGGGCTGATCGGCCGCGGGCTCGCCTATCCGTGCTTCTGCTCACGCGGCCAGATCCGGGCGCGCATCGC
CTCGGGTATCGACAGCCCCCGCGATCCGGACGGCGTGCCCCTCTATCCCGGCACCTGCCGCCACCTGGGCCCGGCGGCGG
GGGACCGGATCGCCCGCGGCGACCCGCATACCTGGCGGCTCGACATGGCGGCCGCGACGCGACGCGCCGGCCCGACGCAC
GGCTACGTCGCCTTCGACGGGACCGGTGCGCACCGGGTCGCGGCGGATCCGACACGCTGGGGCGACGCGGTGATCGCCCG
GCGCGACGTGCCGACGAGCTACCATCTCGCCGTGGTCCACGACGACGCGGTCCAGGGGATCACCCACGTGGTGCGCGGCC
GCGACCTGGAGGCGGCCACCGACCTGCACGTGCTCCTCCAGGCCCTGCTCGGGCTGCCGGTGCCGCGCTACCGCCACCAC
CCGCTGCTGCTCGATGCCGGGGGCGAGAAGCTGTCCAAGTCGCGCGGCTCGCGATCCCTCGCCGACCTCCGGGAGGCCGG
CGCGACGCCCCAGGCTGTCCGCGCCCGGCTCGGCTTCGCCTGA

Upstream 100 bases:

>100_bases
TGCTCGCTGCCCGGGATCGCCTGCCAGCCCGGCGCGCTGCAGTGCCTGCACCGCCGCGACGGCTGATCCGGCGGTCCGGC
CCGTGAGCGCCGAGGCGCCG

Downstream 100 bases:

>100_bases
GCGGCGACGCGCACCCTCAGCGAACCGCCGCGTCGCGCTGGGCGAGGGCTGTGGCGCTCTTCGATTTCTGCATAGTGGCG
TCGATCTGCTCGCGCTGGCG

Product: glutamyl-tRNA synthetase class Ic

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MLRFAPSPNGRLHLGHAYSALLNADLAARMGGVCRLRIEDIDPARSKPDFVHGIVTDLAWLGLTYPEPVRHQSRHMADYR
AALDGLIGRGLAYPCFCSRGQIRARIASGIDSPRDPDGVPLYPGTCRHLGPAAGDRIARGDPHTWRLDMAAATRRAGPTH
GYVAFDGTGAHRVAADPTRWGDAVIARRDVPTSYHLAVVHDDAVQGITHVVRGRDLEAATDLHVLLQALLGLPVPRYRHH
PLLLDAGGEKLSKSRGSRSLADLREAGATPQAVRARLGFA

Sequences:

>Translated_280_residues
MLRFAPSPNGRLHLGHAYSALLNADLAARMGGVCRLRIEDIDPARSKPDFVHGIVTDLAWLGLTYPEPVRHQSRHMADYR
AALDGLIGRGLAYPCFCSRGQIRARIASGIDSPRDPDGVPLYPGTCRHLGPAAGDRIARGDPHTWRLDMAAATRRAGPTH
GYVAFDGTGAHRVAADPTRWGDAVIARRDVPTSYHLAVVHDDAVQGITHVVRGRDLEAATDLHVLLQALLGLPVPRYRHH
PLLLDAGGEKLSKSRGSRSLADLREAGATPQAVRARLGFA
>Mature_280_residues
MLRFAPSPNGRLHLGHAYSALLNADLAARMGGVCRLRIEDIDPARSKPDFVHGIVTDLAWLGLTYPEPVRHQSRHMADYR
AALDGLIGRGLAYPCFCSRGQIRARIASGIDSPRDPDGVPLYPGTCRHLGPAAGDRIARGDPHTWRLDMAAATRRAGPTH
GYVAFDGTGAHRVAADPTRWGDAVIARRDVPTSYHLAVVHDDAVQGITHVVRGRDLEAATDLHVLLQALLGLPVPRYRHH
PLLLDAGGEKLSKSRGSRSLADLREAGATPQAVRARLGFA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=283, Percent_Identity=33.2155477031802, Blast_Score=124, Evalue=7e-29,
Organism=Homo sapiens, GI4826960, Length=93, Percent_Identity=40.8602150537634, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1788743, Length=285, Percent_Identity=33.6842105263158, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI226510925, Length=261, Percent_Identity=36.7816091954023, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1786895, Length=101, Percent_Identity=39.6039603960396, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17543332, Length=101, Percent_Identity=37.6237623762376, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324540, Length=275, Percent_Identity=34.1818181818182, Blast_Score=144, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324742, Length=101, Percent_Identity=32.6732673267327, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24665321, Length=282, Percent_Identity=35.4609929078014, Blast_Score=146, Evalue=2e-35,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020752
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 30265; Mature: 30265

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRFAPSPNGRLHLGHAYSALLNADLAARMGGVCRLRIEDIDPARSKPDFVHGIVTDLAW
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHH
LGLTYPEPVRHQSRHMADYRAALDGLIGRGLAYPCFCSRGQIRARIASGIDSPRDPDGVP
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHCCCCCCCCCCCC
LYPGTCRHLGPAAGDRIARGDPHTWRLDMAAATRRAGPTHGYVAFDGTGAHRVAADPTRW
CCCCCHHHCCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCC
GDAVIARRDVPTSYHLAVVHDDAVQGITHVVRGRDLEAATDLHVLLQALLGLPVPRYRHH
CCCEEEECCCCCCEEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
PLLLDAGGEKLSKSRGSRSLADLREAGATPQAVRARLGFA
CEEEECCCHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure
MLRFAPSPNGRLHLGHAYSALLNADLAARMGGVCRLRIEDIDPARSKPDFVHGIVTDLAW
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHH
LGLTYPEPVRHQSRHMADYRAALDGLIGRGLAYPCFCSRGQIRARIASGIDSPRDPDGVP
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHCCCCCCCCCCCC
LYPGTCRHLGPAAGDRIARGDPHTWRLDMAAATRRAGPTHGYVAFDGTGAHRVAADPTRW
CCCCCHHHCCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCC
GDAVIARRDVPTSYHLAVVHDDAVQGITHVVRGRDLEAATDLHVLLQALLGLPVPRYRHH
CCCEEEECCCCCCEEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
PLLLDAGGEKLSKSRGSRSLADLREAGATPQAVRARLGFA
CEEEECCCHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]