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The map label for this gene is rffG [H]

Identifier: 170748797

GI number: 170748797

Start: 2521586

End: 2522635

Strand: Reverse

Name: rffG [H]

Synonym: Mrad2831_2379

Alternate gene names: 170748797

Gene position: 2522635-2521586 (Counterclockwise)

Preceding gene: 170748798

Following gene: 170748796

Centisome position: 41.51

GC content: 70.19

Gene sequence:

>1050_bases
ATGCGCATCCTGGTGACCGGAGGCTGCGGCTTCATCGGCTCGGCGCTGGTGCTGCACCTCGTCAACGATCTCGGCCACGA
CGTCTGCACCCTCGACGCGATGACCTACGCGGCCAACCCGGTCTCGCTGGCGCCGCTCGCCGACAACCCGCGCCATCGCC
TGGTCGAGGCCGACATCTGCGACCGCGCCGCCGTGCAGAAGGCTTACGCGGACTTCAAGCCCGAGGCGGTGATGCACCTG
GCCGCCGAGAGCCACGTCGACCGCTCGATCACCGACCCGGGCGCCTTCGTGCGCACCAACGTGATCGGCACGCAGACCAT
GCTGGACGGCGCCCGCGGCCATTACGAGACCCTGCCGGAGGCCGAGAAGAGGACCTTCCGCTTCCTCCACGTCTCCACCG
ACGAGGTCTACGGCTCGCTGCCGCCCGACGCGTTCTTCACGGAGGAGAGCCGCTACGATCCGCGCTCGCCCTACTCGGCC
TCCAAGGCGGCCTCCGACCACCTCGCCCGCGCCTGGCACGAGACCTACGGGCTGCCGGTCCTGGTGACCAACTGCTCGAA
CAATTACGGGCCGCGCCACTTCCCCGAGAAGCTGATCCCGCTGATGATCCTCAACGCCCTCGAGGGCAAGAAGCTGCCCG
TTTACGGCGACGGCCTGAACGAGCGCGACTGGATCCACGTGGAGGACCACGCCAAGGGGCTCGTCGCCGTGCTGGAGCGC
GGGCGGATCGGCGAGACCTACCTGCTCGGCGGCCGCGCGGTGCGCAACAACCTCGCGGTGGTCAAGGCGCTGTGCGCGGC
CTTCGACCGGCTGCGCCCGCAGGGCGCCCCGCACGAGCAGCTGATCAGCTTCGTGGCCGACCGCCCGGGCCACGACCGGC
GCTACGCGATCGACTGCACCAAGGCCGAGACCGAGCTCGGCTGGCGCCCGCAGAAGACCTTCGAGCAGGCGCTTGAGGAG
ACCGTGGCGTGGTACCTCGGGAACGAGGGCTGGTGGCGCCCGATCCGCGAGGGCCGCTACAAGGGCGAGCGCCTGGGGCT
CAACGGCTGA

Upstream 100 bases:

>100_bases
CTTCTGAAGAGCGCGGCCCGGCCGCAATTTTGTCACGTCGGCCGGTCTTAGGCTGCCCCGGCTTTCTCAGTTCTCGATCG
ATCCGAAGAGGTAATCCGCC

Downstream 100 bases:

>100_bases
TGGACGTCCTCGTCCTCGGCGGCGCCGGGCAGGTCGGCACCGAGCTTCAGGCCCTGGCTTGGCCGGACGGCGTCACCGTC
CACGCGCCCGGCCGCGCGGA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MRILVTGGCGFIGSALVLHLVNDLGHDVCTLDAMTYAANPVSLAPLADNPRHRLVEADICDRAAVQKAYADFKPEAVMHL
AAESHVDRSITDPGAFVRTNVIGTQTMLDGARGHYETLPEAEKRTFRFLHVSTDEVYGSLPPDAFFTEESRYDPRSPYSA
SKAASDHLARAWHETYGLPVLVTNCSNNYGPRHFPEKLIPLMILNALEGKKLPVYGDGLNERDWIHVEDHAKGLVAVLER
GRIGETYLLGGRAVRNNLAVVKALCAAFDRLRPQGAPHEQLISFVADRPGHDRRYAIDCTKAETELGWRPQKTFEQALEE
TVAWYLGNEGWWRPIREGRYKGERLGLNG

Sequences:

>Translated_349_residues
MRILVTGGCGFIGSALVLHLVNDLGHDVCTLDAMTYAANPVSLAPLADNPRHRLVEADICDRAAVQKAYADFKPEAVMHL
AAESHVDRSITDPGAFVRTNVIGTQTMLDGARGHYETLPEAEKRTFRFLHVSTDEVYGSLPPDAFFTEESRYDPRSPYSA
SKAASDHLARAWHETYGLPVLVTNCSNNYGPRHFPEKLIPLMILNALEGKKLPVYGDGLNERDWIHVEDHAKGLVAVLER
GRIGETYLLGGRAVRNNLAVVKALCAAFDRLRPQGAPHEQLISFVADRPGHDRRYAIDCTKAETELGWRPQKTFEQALEE
TVAWYLGNEGWWRPIREGRYKGERLGLNG
>Mature_349_residues
MRILVTGGCGFIGSALVLHLVNDLGHDVCTLDAMTYAANPVSLAPLADNPRHRLVEADICDRAAVQKAYADFKPEAVMHL
AAESHVDRSITDPGAFVRTNVIGTQTMLDGARGHYETLPEAEKRTFRFLHVSTDEVYGSLPPDAFFTEESRYDPRSPYSA
SKAASDHLARAWHETYGLPVLVTNCSNNYGPRHFPEKLIPLMILNALEGKKLPVYGDGLNERDWIHVEDHAKGLVAVLER
GRIGETYLLGGRAVRNNLAVVKALCAAFDRLRPQGAPHEQLISFVADRPGHDRRYAIDCTKAETELGWRPQKTFEQALEE
TVAWYLGNEGWWRPIREGRYKGERLGLNG

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=338, Percent_Identity=32.5443786982249, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI42516563, Length=335, Percent_Identity=26.865671641791, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=57.7903682719547, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI1788353, Length=351, Percent_Identity=54.4159544159544, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1786974, Length=349, Percent_Identity=28.9398280802292, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1788366, Length=351, Percent_Identity=26.4957264957265, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17568069, Length=328, Percent_Identity=33.2317073170732, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI115532424, Length=336, Percent_Identity=30.952380952381, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI71982035, Length=369, Percent_Identity=29.2682926829268, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71982038, Length=371, Percent_Identity=29.1105121293801, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17539532, Length=330, Percent_Identity=24.5454545454545, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI133901786, Length=342, Percent_Identity=23.6842105263158, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=342, Percent_Identity=23.6842105263158, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=342, Percent_Identity=23.6842105263158, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=342, Percent_Identity=23.6842105263158, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=342, Percent_Identity=23.6842105263158, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17507723, Length=342, Percent_Identity=23.0994152046784, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=375, Percent_Identity=24.8, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356223, Length=337, Percent_Identity=25.5192878338279, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19923002, Length=357, Percent_Identity=27.1708683473389, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24158427, Length=343, Percent_Identity=23.3236151603499, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 38868; Mature: 38868

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVTGGCGFIGSALVLHLVNDLGHDVCTLDAMTYAANPVSLAPLADNPRHRLVEADIC
CEEEEECCCHHHHHHHHHHHHHHHCCCCEEHHHHHHCCCCEEECCCCCCCHHHEEEHHHH
DRAAVQKAYADFKPEAVMHLAAESHVDRSITDPGAFVRTNVIGTQTMLDGARGHYETLPE
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHCCCCCCHHCCCC
AEKRTFRFLHVSTDEVYGSLPPDAFFTEESRYDPRSPYSASKAASDHLARAWHETYGLPV
HHHCEEEEEEEEHHHHCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEE
LVTNCSNNYGPRHFPEKLIPLMILNALEGKKLPVYGDGLNERDWIHVEDHAKGLVAVLER
EEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCEEEHHHHHHHHHHHHC
GRIGETYLLGGRAVRNNLAVVKALCAAFDRLRPQGAPHEQLISFVADRPGHDRRYAIDCT
CCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEEC
KAETELGWRPQKTFEQALEETVAWYLGNEGWWRPIREGRYKGERLGLNG
CCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRILVTGGCGFIGSALVLHLVNDLGHDVCTLDAMTYAANPVSLAPLADNPRHRLVEADIC
CEEEEECCCHHHHHHHHHHHHHHHCCCCEEHHHHHHCCCCEEECCCCCCCHHHEEEHHHH
DRAAVQKAYADFKPEAVMHLAAESHVDRSITDPGAFVRTNVIGTQTMLDGARGHYETLPE
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHCCCCCCHHCCCC
AEKRTFRFLHVSTDEVYGSLPPDAFFTEESRYDPRSPYSASKAASDHLARAWHETYGLPV
HHHCEEEEEEEEHHHHCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEE
LVTNCSNNYGPRHFPEKLIPLMILNALEGKKLPVYGDGLNERDWIHVEDHAKGLVAVLER
EEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCEEEHHHHHHHHHHHHC
GRIGETYLLGGRAVRNNLAVVKALCAAFDRLRPQGAPHEQLISFVADRPGHDRRYAIDCT
CCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEEC
KAETELGWRPQKTFEQALEETVAWYLGNEGWWRPIREGRYKGERLGLNG
CCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]