Click here to switch to the map view.

The map label for this gene is rfbD [C]

Identifier: 170748796

GI number: 170748796

Start: 2520681

End: 2521586

Strand: Reverse

Name: rfbD [C]

Synonym: Mrad2831_2378

Alternate gene names: 170748796

Gene position: 2521586-2520681 (Counterclockwise)

Preceding gene: 170748797

Following gene: 170748795

Centisome position: 41.49

GC content: 75.72

Gene sequence:

>906_bases
ATGGACGTCCTCGTCCTCGGCGGCGCCGGGCAGGTCGGCACCGAGCTTCAGGCCCTGGCTTGGCCGGACGGCGTCACCGT
CCACGCGCCCGGCCGCGCGGAGCTCGACATCACCGACGCGGACGCCGTCGCGGCGGCGCTCGCGGCCCGCGCCTACCGGG
CGGTGATCAACACCGCCGCCTACACGGCGGTCGACAAGGCCGAGTCCGACGTGGTGGCGGCGTGGCGCCTCAACGCCCTC
GCCCCGGCGATCCTGGCGGCCGCCACGGCGGCCCGATCCATCCCGCTGGTGCACGTCTCGACCGACTACGTCTTCGCCGG
GACCAAGCCCGACGGGGCCTACGCGCCCGACGCTCCGATCGACCCGCAGAGCGTCTACGGCGCCAGCAAGGCCGCCGGCG
AGCTGGCGGTGCGCACCGGCAACCCGCGCCACGCCGTCGTCCGGACCGCCTGGGTGGTGAGCCCGCACCGGGGCAACTTC
GTCAAGACGATGCTGCGGCTCGCGGCCGAGCGCGACGCCCTGACCGTGGTGAACGACCAGCACGGCTGCCCGACCTCGGC
CGCCGACCTCGCCGCCGCCCTCGCGACGATCGCGCAGGCCATGGCGGCGGATCCGGAGGCGCCCGCCGGCACGTTCCACT
GCGTGAACCGGGGCGACACCACGTGGTGCGGCTTCGCCGAGGCGATCGTGGCGGGCTCGGCCCGGCGCGGCGGCCGCGCC
GTGCCGGTCAAGGGCATCCCGACCTCCGCCTACCCGACACCGGCCCGGCGGCCGGCCAATTCGCGCCTGTCCACCGACAG
CCTCACGGCCGCCTACGGCATCGCGCCGCGGCCCTGGCAGGCGGCGCTCGACGACATCCTGGACCGGCTCGTCGGGCCGG
TCTCAGAGGGAGTTTCCAAGCCATGA

Upstream 100 bases:

>100_bases
CGCTTGAGGAGACCGTGGCGTGGTACCTCGGGAACGAGGGCTGGTGGCGCCCGATCCGCGAGGGCCGCTACAAGGGCGAG
CGCCTGGGGCTCAACGGCTG

Downstream 100 bases:

>100_bases
AGGGCATCGTCCTGGCCGGCGGATCCGGCACGCGCCTGCACCCGGCCACGCTGTCGATCAACAAGCAGCTCCTGCCGGTC
TACGACAAGCCGATGATCTA

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MDVLVLGGAGQVGTELQALAWPDGVTVHAPGRAELDITDADAVAAALAARAYRAVINTAAYTAVDKAESDVVAAWRLNAL
APAILAAATAARSIPLVHVSTDYVFAGTKPDGAYAPDAPIDPQSVYGASKAAGELAVRTGNPRHAVVRTAWVVSPHRGNF
VKTMLRLAAERDALTVVNDQHGCPTSAADLAAALATIAQAMAADPEAPAGTFHCVNRGDTTWCGFAEAIVAGSARRGGRA
VPVKGIPTSAYPTPARRPANSRLSTDSLTAAYGIAPRPWQAALDDILDRLVGPVSEGVSKP

Sequences:

>Translated_301_residues
MDVLVLGGAGQVGTELQALAWPDGVTVHAPGRAELDITDADAVAAALAARAYRAVINTAAYTAVDKAESDVVAAWRLNAL
APAILAAATAARSIPLVHVSTDYVFAGTKPDGAYAPDAPIDPQSVYGASKAAGELAVRTGNPRHAVVRTAWVVSPHRGNF
VKTMLRLAAERDALTVVNDQHGCPTSAADLAAALATIAQAMAADPEAPAGTFHCVNRGDTTWCGFAEAIVAGSARRGGRA
VPVKGIPTSAYPTPARRPANSRLSTDSLTAAYGIAPRPWQAALDDILDRLVGPVSEGVSKP
>Mature_301_residues
MDVLVLGGAGQVGTELQALAWPDGVTVHAPGRAELDITDADAVAAALAARAYRAVINTAAYTAVDKAESDVVAAWRLNAL
APAILAAATAARSIPLVHVSTDYVFAGTKPDGAYAPDAPIDPQSVYGASKAAGELAVRTGNPRHAVVRTAWVVSPHRGNF
VKTMLRLAAERDALTVVNDQHGCPTSAADLAAALATIAQAMAADPEAPAGTFHCVNRGDTTWCGFAEAIVAGSARRGGRA
VPVKGIPTSAYPTPARRPANSRLSTDSLTAAYGIAPRPWQAALDDILDRLVGPVSEGVSKP

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=297, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI11034825, Length=297, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1788352, Length=298, Percent_Identity=38.255033557047, Blast_Score=178, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 30866; Mature: 30866

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVLVLGGAGQVGTELQALAWPDGVTVHAPGRAELDITDADAVAAALAARAYRAVINTAA
CCEEEECCCCCCCCCEEEEECCCCEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
YTAVDKAESDVVAAWRLNALAPAILAAATAARSIPLVHVSTDYVFAGTKPDGAYAPDAPI
HHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCCCCCCCCCCCCC
DPQSVYGASKAAGELAVRTGNPRHAVVRTAWVVSPHRGNFVKTMLRLAAERDALTVVNDQ
CHHHHCCCCCCCCEEEEECCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECC
HGCPTSAADLAAALATIAQAMAADPEAPAGTFHCVNRGDTTWCGFAEAIVAGSARRGGRA
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEE
VPVKGIPTSAYPTPARRPANSRLSTDSLTAAYGIAPRPWQAALDDILDRLVGPVSEGVSK
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
P
C
>Mature Secondary Structure
MDVLVLGGAGQVGTELQALAWPDGVTVHAPGRAELDITDADAVAAALAARAYRAVINTAA
CCEEEECCCCCCCCCEEEEECCCCEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
YTAVDKAESDVVAAWRLNALAPAILAAATAARSIPLVHVSTDYVFAGTKPDGAYAPDAPI
HHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEECCCCCCCCCCCCCC
DPQSVYGASKAAGELAVRTGNPRHAVVRTAWVVSPHRGNFVKTMLRLAAERDALTVVNDQ
CHHHHCCCCCCCCEEEEECCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECC
HGCPTSAADLAAALATIAQAMAADPEAPAGTFHCVNRGDTTWCGFAEAIVAGSARRGGRA
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEE
VPVKGIPTSAYPTPARRPANSRLSTDSLTAAYGIAPRPWQAALDDILDRLVGPVSEGVSK
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]