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The map label for this gene is gloA [H]

Identifier: 170748784

GI number: 170748784

Start: 2506692

End: 2507132

Strand: Reverse

Name: gloA [H]

Synonym: Mrad2831_2366

Alternate gene names: 170748784

Gene position: 2507132-2506692 (Counterclockwise)

Preceding gene: 170748788

Following gene: 170748783

Centisome position: 41.25

GC content: 65.31

Gene sequence:

>441_bases
ATGGAATTTCTGCACACCATGGTCCGCGTCGCCGACCTCGACCGGGCGCTCGCCTTCTACGTCGACACGTTCGGCCTGAA
GGAGGTGCGCCGGGTCGAGAACGAGAAGGGCCGCTTCACCCTCGTGTTCCTCGCCGCGCCCGGTGATGTCGAGCGGGCCG
CGGCGACCAAGTCGCCGCTGATCGAGCTCACGTACAACTGGGATCCGGAGACCTACTCGGGCGGGCGCAATTTCGGCCAT
CTCGCCTATCAGGTCGACGACATCTACGGCTTCTGCGCCAGGCTGCAGGAGAAGGGCGTCACCATCAACCGGCCACCGCG
CGACGGCTACATGGCCTTCGTGAAGTCGCCGGACGGCATTTCCATCGAGATCCTCCAGAAGGGCGGCGCCAAGGAGCCGC
AGGAGCCGTGGAAGTCGATGGAGAACACCGGCACCTGGTAG

Upstream 100 bases:

>100_bases
CGGGCGGCCTTCTCACCGCGGGAGTGGCCGCTAGCTAGCGGGACGAGTCAGCCGGGGACGGGCCAGCGGTCCGTTTTCGA
AGATCACAGGACAGGTCCCG

Downstream 100 bases:

>100_bases
CGGGCCCGCACCCTTGACCCGGACGGCCCCGCGGGCGCATCTGCCCGCGGATCAGCCGGCCGGGCAGCCGCTCCGGACGC
GCGAGCGTTCGGGGAGGAAA

Product: glyoxalase/bleomycin resistance protein/dioxygenase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MEFLHTMVRVADLDRALAFYVDTFGLKEVRRVENEKGRFTLVFLAAPGDVERAAATKSPLIELTYNWDPETYSGGRNFGH
LAYQVDDIYGFCARLQEKGVTINRPPRDGYMAFVKSPDGISIEILQKGGAKEPQEPWKSMENTGTW

Sequences:

>Translated_146_residues
MEFLHTMVRVADLDRALAFYVDTFGLKEVRRVENEKGRFTLVFLAAPGDVERAAATKSPLIELTYNWDPETYSGGRNFGH
LAYQVDDIYGFCARLQEKGVTINRPPRDGYMAFVKSPDGISIEILQKGGAKEPQEPWKSMENTGTW
>Mature_146_residues
MEFLHTMVRVADLDRALAFYVDTFGLKEVRRVENEKGRFTLVFLAAPGDVERAAATKSPLIELTYNWDPETYSGGRNFGH
LAYQVDDIYGFCARLQEKGVTINRPPRDGYMAFVKSPDGISIEILQKGGAKEPQEPWKSMENTGTW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=143, Percent_Identity=33.5664335664336, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1787940, Length=132, Percent_Identity=36.3636363636364, Blast_Score=92, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323639, Length=142, Percent_Identity=33.0985915492958, Blast_Score=77, Evalue=1e-15,
Organism=Drosophila melanogaster, GI20129743, Length=145, Percent_Identity=35.8620689655172, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 16523; Mature: 16523

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFLHTMVRVADLDRALAFYVDTFGLKEVRRVENEKGRFTLVFLAAPGDVERAAATKSPL
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEEECCCCHHHHHCCCCCE
IELTYNWDPETYSGGRNFGHLAYQVDDIYGFCARLQEKGVTINRPPRDGYMAFVKSPDGI
EEEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCEECCCCCCCEEEEEECCCCC
SIEILQKGGAKEPQEPWKSMENTGTW
EEEEEECCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MEFLHTMVRVADLDRALAFYVDTFGLKEVRRVENEKGRFTLVFLAAPGDVERAAATKSPL
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEEECCCCHHHHHCCCCCE
IELTYNWDPETYSGGRNFGHLAYQVDDIYGFCARLQEKGVTINRPPRDGYMAFVKSPDGI
EEEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCEECCCCCCCEEEEEECCCCC
SIEILQKGGAKEPQEPWKSMENTGTW
EEEEEECCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]