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The map label for this gene is mraW

Identifier: 170748783

GI number: 170748783

Start: 2504754

End: 2505815

Strand: Reverse

Name: mraW

Synonym: Mrad2831_2365

Alternate gene names: 170748783

Gene position: 2505815-2504754 (Counterclockwise)

Preceding gene: 170748784

Following gene: 170748782

Centisome position: 41.23

GC content: 75.71

Gene sequence:

>1062_bases
ATGAGCCGGCGCCGCCCCGCTCCCGAGAGTTCGCCCCGTCACGACGGCGCCGATGCGCCGCCCGAGCCGCACGTGCCCGT
CCTCCTCGCGGAGGTCGTCGAGGCGCTCGGCACCGAGGGCGGGACCGCGGTCGACGGCACGTTCGGTGCCGGCGGCTACA
CCCGCGCGCTGCTCGCGGCCGATCCGGCCCTGCAGGTCGTCGCGATCGATCGCGACCCCACGGCGATCGCGGGCGGGCAG
GCGCTCGTCGCCGAGTCCGGCGGGCGCCTGCGCCTCCTGCCCGGCCGCTTCGGCGACCTCGACGGCCTCCTCGCGGAGGC
CGGTATCGCGCAGGTCGACCGGGTCGTCCTCGATATCGGGGTCTCGTCGATGCAGCTCGACGCGCCGGAGCGGGGCTTCT
CGTTCCGCAGCGACGGCCCCCTCGACATGCGCATGGCCTGCGACGGGCCGAGCGCCGCCGACCTCGTGAACGAGGCCGAC
GAGACCGTTCTGGCCGACATCATCTACCATTTCGGCGAGGAGCGGCGCTCGCGGGCGGTCGCGCGGACGATCCTCGAGGC
GCGCCGCCGGGGGCGGATCGAGACCACCGCGCAGCTGGCCGAGCTCGTCGCCGGCGTGGTGCGGACCGAGCCCGGCAGCC
ACATCCACCCGGCGACGCGGACCTTCCAGGGCCTGCGGATCGCCGTCAACGACGAGCTCGGCGAGCTGGTCCGTGCCCTG
CACGCGGCCGAGCGCGTGCTCAGGCCCGGCGGCCGGCTCGCCGTCGTGACGTTCCACTCGCTCGAGGATCGGATCGTCAA
GCAGTTCTTCGCGGCGCGCAGCGGCCGCTCAGCGCAGGGCTCGCGCCATCTGCCCGGCGGCCCCGTCGAGACCGTCCGCA
GCTTCCGCCCCGTCACCAAGGGTCCGGTCCTGCCGGGCGAGGCCGAGACTGCCCGGAACCCGCGCGCCCGCTCGGCCAAG
CTCCGGGCCGCGGAGCGCACCGAATCCGACGTTCCCGAACCGCTGGCCGCGCTGACCGCGCTGGCGAGCCTGCCCGACGC
GGCCCGAGGCCATCGCCGATGA

Upstream 100 bases:

>100_bases
CCATGAGACGCACGAGCCGCATCCGCGCCCGGATTTCCGGGACGCGCCGACAATCCGCCCGTCCTCGCGCGCCGCGCCGC
GCCGCGCCCCCGAGGCGGTC

Downstream 100 bases:

>100_bases
TCCGCCTCCTGAACCTGCTGGCGGTGGCCGGCCTCGTCGCCTCGGCGATCTACGCCTACTCGATCAAGTACGACACGCTC
TACCAGGGCGGTCAGGTCTC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MSRRRPAPESSPRHDGADAPPEPHVPVLLAEVVEALGTEGGTAVDGTFGAGGYTRALLAADPALQVVAIDRDPTAIAGGQ
ALVAESGGRLRLLPGRFGDLDGLLAEAGIAQVDRVVLDIGVSSMQLDAPERGFSFRSDGPLDMRMACDGPSAADLVNEAD
ETVLADIIYHFGEERRSRAVARTILEARRRGRIETTAQLAELVAGVVRTEPGSHIHPATRTFQGLRIAVNDELGELVRAL
HAAERVLRPGGRLAVVTFHSLEDRIVKQFFAARSGRSAQGSRHLPGGPVETVRSFRPVTKGPVLPGEAETARNPRARSAK
LRAAERTESDVPEPLAALTALASLPDAARGHRR

Sequences:

>Translated_353_residues
MSRRRPAPESSPRHDGADAPPEPHVPVLLAEVVEALGTEGGTAVDGTFGAGGYTRALLAADPALQVVAIDRDPTAIAGGQ
ALVAESGGRLRLLPGRFGDLDGLLAEAGIAQVDRVVLDIGVSSMQLDAPERGFSFRSDGPLDMRMACDGPSAADLVNEAD
ETVLADIIYHFGEERRSRAVARTILEARRRGRIETTAQLAELVAGVVRTEPGSHIHPATRTFQGLRIAVNDELGELVRAL
HAAERVLRPGGRLAVVTFHSLEDRIVKQFFAARSGRSAQGSRHLPGGPVETVRSFRPVTKGPVLPGEAETARNPRARSAK
LRAAERTESDVPEPLAALTALASLPDAARGHRR
>Mature_352_residues
SRRRPAPESSPRHDGADAPPEPHVPVLLAEVVEALGTEGGTAVDGTFGAGGYTRALLAADPALQVVAIDRDPTAIAGGQA
LVAESGGRLRLLPGRFGDLDGLLAEAGIAQVDRVVLDIGVSSMQLDAPERGFSFRSDGPLDMRMACDGPSAADLVNEADE
TVLADIIYHFGEERRSRAVARTILEARRRGRIETTAQLAELVAGVVRTEPGSHIHPATRTFQGLRIAVNDELGELVRALH
AAERVLRPGGRLAVVTFHSLEDRIVKQFFAARSGRSAQGSRHLPGGPVETVRSFRPVTKGPVLPGEAETARNPRARSAKL
RAAERTESDVPEPLAALTALASLPDAARGHRR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=336, Percent_Identity=42.2619047619048, Blast_Score=231, Evalue=6e-61,
Organism=Homo sapiens, GI165377202, Length=193, Percent_Identity=43.5233160621762, Blast_Score=153, Evalue=3e-37,
Organism=Escherichia coli, GI1786270, Length=316, Percent_Identity=43.6708860759494, Blast_Score=212, Evalue=2e-56,
Organism=Drosophila melanogaster, GI62472493, Length=341, Percent_Identity=40.7624633431085, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI28571637, Length=341, Percent_Identity=40.7624633431085, Blast_Score=210, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_METRJ (B1LYT9)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001755043.1
- ProteinModelPortal:   B1LYT9
- SMR:   B1LYT9
- GeneID:   6138397
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_2365
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 37440; Mature: 37309

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: BINDING 70-70 BINDING 97-97 BINDING 118-118 BINDING 125-125

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRRPAPESSPRHDGADAPPEPHVPVLLAEVVEALGTEGGTAVDGTFGAGGYTRALLAA
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHC
DPALQVVAIDRDPTAIAGGQALVAESGGRLRLLPGRFGDLDGLLAEAGIAQVDRVVLDIG
CCCEEEEEECCCCCEECCCCEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
VSSMQLDAPERGFSFRSDGPLDMRMACDGPSAADLVNEADETVLADIIYHFGEERRSRAV
CCCEECCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARTILEARRRGRIETTAQLAELVAGVVRTEPGSHIHPATRTFQGLRIAVNDELGELVRAL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEECHHHHHHHHHH
HAAERVLRPGGRLAVVTFHSLEDRIVKQFFAARSGRSAQGSRHLPGGPVETVRSFRPVTK
HHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCC
GPVLPGEAETARNPRARSAKLRAAERTESDVPEPLAALTALASLPDAARGHRR
CCCCCCCCHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHCCCCC
>Mature Secondary Structure 
SRRRPAPESSPRHDGADAPPEPHVPVLLAEVVEALGTEGGTAVDGTFGAGGYTRALLAA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHC
DPALQVVAIDRDPTAIAGGQALVAESGGRLRLLPGRFGDLDGLLAEAGIAQVDRVVLDIG
CCCEEEEEECCCCCEECCCCEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
VSSMQLDAPERGFSFRSDGPLDMRMACDGPSAADLVNEADETVLADIIYHFGEERRSRAV
CCCEECCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARTILEARRRGRIETTAQLAELVAGVVRTEPGSHIHPATRTFQGLRIAVNDELGELVRAL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEECHHHHHHHHHH
HAAERVLRPGGRLAVVTFHSLEDRIVKQFFAARSGRSAQGSRHLPGGPVETVRSFRPVTK
HHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCC
GPVLPGEAETARNPRARSAKLRAAERTESDVPEPLAALTALASLPDAARGHRR
CCCCCCCCHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA