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The map label for this gene is argF [H]

Identifier: 170748735

GI number: 170748735

Start: 2453956

End: 2454936

Strand: Reverse

Name: argF [H]

Synonym: Mrad2831_2317

Alternate gene names: 170748735

Gene position: 2454936-2453956 (Counterclockwise)

Preceding gene: 170748736

Following gene: 170748734

Centisome position: 40.39

GC content: 70.34

Gene sequence:

>981_bases
ATGAAAGCCCAGATTGACGGCCGGGCACACGCCCCGGCCAGGCACCTGAACGGTGCCGGCCCTCGTCATTTCCTCGATCT
CAAGGATTTCTCCGGGGCGGAGCTGCGCCGGGTCCTCGATGCCAGCGCCGCCATCAAGGCGCGCCGCCGCAAGGGCGAGC
TGGCTGCCGAGCGCCCGCTCACCGGCAAGACCCTCGCCATGGTGTTCGACCGGCCCTCGACCCGCACCCGCGTCTCCTTC
GACGTGGCGATGCGCGAGCTCGGCGGCGAGACCCTGATGCTCACCGGCTCCGAGATGCAGCTCGGCCGCGGCGAGACCAT
CGGCGACACCGCGCAGGTGCTCTCGCGCTTCGTCGACGCGATCATGATCCGCATCCTCGACCACGGTCAGATGCTGGAGC
TGGCCGAGTACGCCACCGTCCCGGTGATCAACGCGCTGACCAAGGTCTCGCACCCGTGCCAGATCATGGCCGACGTGCTG
ACCTTCGAGGAGCATCGCGGGCCGATCCGGGGCCGCAAGGTCGCGTGGTCGGGCGATGCGAACAACGTGCTGGCGAGCTG
GGTTCACGCCGCCGCCCGGTTCGACTTCACCCTGAACATCGCGAGCCCGCCGGAACTCGCGCCGCCGCCGGCGCTCCTCG
CCTGGGCGCAGCAGGAGGGTGCCGACGTCCACGTCACCACCGACGCCTTCGCGGCCGTGGACGGCGTCGATGCGGTGGTC
ACCGATTGCTGGGTGTCGATGGGCGACGACGACGAGGCGCACCGGCACAACCTGCTGAGCCCCTATCAGGTCAACGACGC
GCTGATGGCGGCGGCGAACCCGGACGCGATCTTCATGCACTGCCTCCCGGCCCACCGCGGCGAGGAGGTGACGGCCGAGG
TGATGGACGGCCCGCGCTCCGTGGTGTTCGACGAGGCCGAGAACCGCCTGCACGCGCAGAAGGGCATCCTGGCCTGGTGC
CTGGGCGCCAGCGGGCTCTGA

Upstream 100 bases:

>100_bases
GCGGTCGATCCCGCCGCGCGTGACGGAGATGCCCCTGCCAGGAGGTAGAGTGCGGTTCATGATCAGGTGATCGTTGAGCA
GACTCTAGTATTGTGACGCG

Downstream 100 bases:

>100_bases
GCCCGCGGTCGCCACGTCCGGCCTCGCGCTCGCGGGGTCGGGGCACCATATAGGCGTGAGCGGGCGGCCCGTGCCGTCCG
TCTCCCCATCGCGGCCGCCG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKAQIDGRAHAPARHLNGAGPRHFLDLKDFSGAELRRVLDASAAIKARRRKGELAAERPLTGKTLAMVFDRPSTRTRVSF
DVAMRELGGETLMLTGSEMQLGRGETIGDTAQVLSRFVDAIMIRILDHGQMLELAEYATVPVINALTKVSHPCQIMADVL
TFEEHRGPIRGRKVAWSGDANNVLASWVHAAARFDFTLNIASPPELAPPPALLAWAQQEGADVHVTTDAFAAVDGVDAVV
TDCWVSMGDDDEAHRHNLLSPYQVNDALMAAANPDAIFMHCLPAHRGEEVTAEVMDGPRSVVFDEAENRLHAQKGILAWC
LGASGL

Sequences:

>Translated_326_residues
MKAQIDGRAHAPARHLNGAGPRHFLDLKDFSGAELRRVLDASAAIKARRRKGELAAERPLTGKTLAMVFDRPSTRTRVSF
DVAMRELGGETLMLTGSEMQLGRGETIGDTAQVLSRFVDAIMIRILDHGQMLELAEYATVPVINALTKVSHPCQIMADVL
TFEEHRGPIRGRKVAWSGDANNVLASWVHAAARFDFTLNIASPPELAPPPALLAWAQQEGADVHVTTDAFAAVDGVDAVV
TDCWVSMGDDDEAHRHNLLSPYQVNDALMAAANPDAIFMHCLPAHRGEEVTAEVMDGPRSVVFDEAENRLHAQKGILAWC
LGASGL
>Mature_326_residues
MKAQIDGRAHAPARHLNGAGPRHFLDLKDFSGAELRRVLDASAAIKARRRKGELAAERPLTGKTLAMVFDRPSTRTRVSF
DVAMRELGGETLMLTGSEMQLGRGETIGDTAQVLSRFVDAIMIRILDHGQMLELAEYATVPVINALTKVSHPCQIMADVL
TFEEHRGPIRGRKVAWSGDANNVLASWVHAAARFDFTLNIASPPELAPPPALLAWAQQEGADVHVTTDAFAAVDGVDAVV
TDCWVSMGDDDEAHRHNLLSPYQVNDALMAAANPDAIFMHCLPAHRGEEVTAEVMDGPRSVVFDEAENRLHAQKGILAWC
LGASGL

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=294, Percent_Identity=39.1156462585034, Blast_Score=211, Evalue=7e-55,
Organism=Homo sapiens, GI18105007, Length=314, Percent_Identity=26.4331210191083, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI1790703, Length=334, Percent_Identity=40.1197604790419, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1786469, Length=331, Percent_Identity=39.5770392749245, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI2367364, Length=311, Percent_Identity=28.6173633440514, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI48994908, Length=330, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=341, Percent_Identity=26.9794721407625, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322373, Length=309, Percent_Identity=33.6569579288026, Blast_Score=181, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6322331, Length=311, Percent_Identity=27.3311897106109, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24642586, Length=312, Percent_Identity=26.6025641025641, Blast_Score=108, Evalue=6e-24,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 35350; Mature: 35350

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAQIDGRAHAPARHLNGAGPRHFLDLKDFSGAELRRVLDASAAIKARRRKGELAAERPL
CCCCCCCCCCCCHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCC
TGKTLAMVFDRPSTRTRVSFDVAMRELGGETLMLTGSEMQLGRGETIGDTAQVLSRFVDA
CCCEEEEEECCCCCCEEEHHHHHHHHCCCCEEEEECCCEECCCCCCCCHHHHHHHHHHHH
IMIRILDHGQMLELAEYATVPVINALTKVSHPCQIMADVLTFEEHRGPIRGRKVAWSGDA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCC
NNVLASWVHAAARFDFTLNIASPPELAPPPALLAWAQQEGADVHVTTDAFAAVDGVDAVV
HHHHHHHHHHHHHEEEEEEECCCCCCCCCCHHHHHHHCCCCEEEEECHHHHHHCCHHHHH
TDCWVSMGDDDEAHRHNLLSPYQVNDALMAAANPDAIFMHCLPAHRGEEVTAEVMDGPRS
HHHHHCCCCCCHHHHHCCCCCEECCCCEEECCCCCEEEEEEECCCCCCCCHHHHHCCCCC
VVFDEAENRLHAQKGILAWCLGASGL
EEEHHHHHHHHHHHCCEEEECCCCCC
>Mature Secondary Structure
MKAQIDGRAHAPARHLNGAGPRHFLDLKDFSGAELRRVLDASAAIKARRRKGELAAERPL
CCCCCCCCCCCCHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHCCCC
TGKTLAMVFDRPSTRTRVSFDVAMRELGGETLMLTGSEMQLGRGETIGDTAQVLSRFVDA
CCCEEEEEECCCCCCEEEHHHHHHHHCCCCEEEEECCCEECCCCCCCCHHHHHHHHHHHH
IMIRILDHGQMLELAEYATVPVINALTKVSHPCQIMADVLTFEEHRGPIRGRKVAWSGDA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCC
NNVLASWVHAAARFDFTLNIASPPELAPPPALLAWAQQEGADVHVTTDAFAAVDGVDAVV
HHHHHHHHHHHHHEEEEEEECCCCCCCCCCHHHHHHHCCCCEEEEECHHHHHHCCHHHHH
TDCWVSMGDDDEAHRHNLLSPYQVNDALMAAANPDAIFMHCLPAHRGEEVTAEVMDGPRS
HHHHHCCCCCCHHHHHCCCCCEECCCCEEECCCCCEEEEEEECCCCCCCCHHHHHCCCCC
VVFDEAENRLHAQKGILAWCLGASGL
EEEHHHHHHHHHHHCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA