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The map label for this gene is trpB

Identifier: 170748290

GI number: 170748290

Start: 2000181

End: 2001422

Strand: Reverse

Name: trpB

Synonym: Mrad2831_1872

Alternate gene names: 170748290

Gene position: 2001422-2000181 (Counterclockwise)

Preceding gene: 170748292

Following gene: 170748286

Centisome position: 32.93

GC content: 69.97

Gene sequence:

>1242_bases
GTGACCATCGCGCCCGAGCCCAACTCCTTCCGCACCGGCCCCGACGAGCGCGGCCGCTTTGGCATCTTCGGCGGCCGCTT
CGTCGCCGAGACGCTGATGCCGCTGATCCTCGAGCTCGAGGCGGCCTACGAGACGGCCAAGAAGGATCCGGCCTTCCAGG
CCGAGATGGAGAGCTACGGCACGCACTATATCGGCCGGCCGAGCCCGCTCTACTACGCCGAGCGCCTGACCGAGCACCTG
CGCGCCAAGGCGCCGGCGGGGCAGGGCGCCAAGGTCTATTTCAAGCGCGAGGAGCTGAACCATACCGGCTCCCACAAGGT
GAACAACGTGCTGGGCCAGATCCTGCTGGCCCGCCGCATGGGCAAGCCGCGGATCATCGCCGAGACCGGCGCCGGCCAGC
ACGGCGTCGCCACCGCGACGCTCTGCGCGCGCTTCGGCCTCAAGTGCGTCGTCTACATGGGCGCGGTCGACGTCGCCCGG
CAGGCGCCCAACGTCTTCCGCATGAAGATGCTCGGCGCCGAGGTGGTCCCGGTGGAATCCGGCACCAAGACCCTCAAGGA
CGCCATGAACGAGGCGCTGCGCGACTGGGTCACCAACGTCGCCGACACGTTCTACTGCATCGGCACGGTGGCGGGCCCGC
ACCCGTACCCGGCGATGGTGCGCGACTTCCAGTCGGTGATCGGCCGCGAGACCCGCGAGCAGATGATCGCGCAGGAGGGC
CGGATCCCGGACTCCCTCGTCGCCTGCATCGGCGGCGGCTCGAACGCCATGGGCCTGTTCCACCCGTTCCTCGACGACCG
CGAGGTCGAGATCTTCGGCGTCGAGGCCGCGGGCCACGGCGTCCAGTCGGGCCTGCACGCGGCCTCGCTGACCGGCGGCA
AGCCGGGCGTGCTGCACGGCAACCGCACCTACCTGCTGATGGACGGGGACGGCCAGATCGCCGACGCCCACTCGATCTCG
GCCGGCCTCGACTACCCGGGCATCGGCCCCGAGCACGCGTGGCTGCACGAGATGGGCCGGGTCACCTACCTGTCGGCGAC
CGATTCCGAGACGCTGGAGGCGTTCAAGCTCTGCTCGATGCTGGAGGGGATCATCCCCGCCCTGGAGCCGGCCCACGCCC
TGTCCAAGGTCCTGGAGCTCGCGCCCCAGCGCCCGGCCGAGCACCTCATGGTGATGAACATGTCGGGCCGCGGCGACAAG
GACATCCCGCAGGTGGCCGAGATCTTCGGCACGAAGATCTGA

Upstream 100 bases:

>100_bases
GCACCCGTTCCGGTTACGCGCGCGGCAGGCCGCATTGCGGTGGCGGCCGCATGGGTCTAGGCACCCCGCAAATCATCGCT
TCGCCCGCAGGATCCGTCCC

Downstream 100 bases:

>100_bases
GGACGCGCGGCGGCGGTCCTCCGCCCGCCGCGCCCCGCCTGCCCCGCGCGCGCACCGTCGGCCTCAGGCGGTCTCGATGT
CCGTGAGCGGGAAGTCGTTG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MTIAPEPNSFRTGPDERGRFGIFGGRFVAETLMPLILELEAAYETAKKDPAFQAEMESYGTHYIGRPSPLYYAERLTEHL
RAKAPAGQGAKVYFKREELNHTGSHKVNNVLGQILLARRMGKPRIIAETGAGQHGVATATLCARFGLKCVVYMGAVDVAR
QAPNVFRMKMLGAEVVPVESGTKTLKDAMNEALRDWVTNVADTFYCIGTVAGPHPYPAMVRDFQSVIGRETREQMIAQEG
RIPDSLVACIGGGSNAMGLFHPFLDDREVEIFGVEAAGHGVQSGLHAASLTGGKPGVLHGNRTYLLMDGDGQIADAHSIS
AGLDYPGIGPEHAWLHEMGRVTYLSATDSETLEAFKLCSMLEGIIPALEPAHALSKVLELAPQRPAEHLMVMNMSGRGDK
DIPQVAEIFGTKI

Sequences:

>Translated_413_residues
MTIAPEPNSFRTGPDERGRFGIFGGRFVAETLMPLILELEAAYETAKKDPAFQAEMESYGTHYIGRPSPLYYAERLTEHL
RAKAPAGQGAKVYFKREELNHTGSHKVNNVLGQILLARRMGKPRIIAETGAGQHGVATATLCARFGLKCVVYMGAVDVAR
QAPNVFRMKMLGAEVVPVESGTKTLKDAMNEALRDWVTNVADTFYCIGTVAGPHPYPAMVRDFQSVIGRETREQMIAQEG
RIPDSLVACIGGGSNAMGLFHPFLDDREVEIFGVEAAGHGVQSGLHAASLTGGKPGVLHGNRTYLLMDGDGQIADAHSIS
AGLDYPGIGPEHAWLHEMGRVTYLSATDSETLEAFKLCSMLEGIIPALEPAHALSKVLELAPQRPAEHLMVMNMSGRGDK
DIPQVAEIFGTKI
>Mature_412_residues
TIAPEPNSFRTGPDERGRFGIFGGRFVAETLMPLILELEAAYETAKKDPAFQAEMESYGTHYIGRPSPLYYAERLTEHLR
AKAPAGQGAKVYFKREELNHTGSHKVNNVLGQILLARRMGKPRIIAETGAGQHGVATATLCARFGLKCVVYMGAVDVARQ
APNVFRMKMLGAEVVPVESGTKTLKDAMNEALRDWVTNVADTFYCIGTVAGPHPYPAMVRDFQSVIGRETREQMIAQEGR
IPDSLVACIGGGSNAMGLFHPFLDDREVEIFGVEAAGHGVQSGLHAASLTGGKPGVLHGNRTYLLMDGDGQIADAHSISA
GLDYPGIGPEHAWLHEMGRVTYLSATDSETLEAFKLCSMLEGIIPALEPAHALSKVLELAPQRPAEHLMVMNMSGRGDKD
IPQVAEIFGTKI

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=394, Percent_Identity=52.2842639593909, Blast_Score=428, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6321412, Length=393, Percent_Identity=57.5063613231552, Blast_Score=472, Evalue=1e-134,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_METRJ (B1LSI6)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001754550.1
- ProteinModelPortal:   B1LSI6
- SMR:   B1LSI6
- GeneID:   6137901
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_1872
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 44683; Mature: 44552

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAPEPNSFRTGPDERGRFGIFGGRFVAETLMPLILELEAAYETAKKDPAFQAEMESYG
CCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
THYIGRPSPLYYAERLTEHLRAKAPAGQGAKVYFKREELNHTGSHKVNNVLGQILLARRM
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHC
GKPRIIAETGAGQHGVATATLCARFGLKCVVYMGAVDVARQAPNVFRMKMLGAEVVPVES
CCCEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHCCEEEECCC
GTKTLKDAMNEALRDWVTNVADTFYCIGTVAGPHPYPAMVRDFQSVIGRETREQMIAQEG
CHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHC
RIPDSLVACIGGGSNAMGLFHPFLDDREVEIFGVEAAGHGVQSGLHAASLTGGKPGVLHG
CCCHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEEECCCCHHHHCCHHHHCCCCCCCEEEC
NRTYLLMDGDGQIADAHSISAGLDYPGIGPEHAWLHEMGRVTYLSATDSETLEAFKLCSM
CEEEEEECCCCCEECCHHCCCCCCCCCCCCCHHHHHHCCCEEEEECCCHHHHHHHHHHHH
LEGIIPALEPAHALSKVLELAPQRPAEHLMVMNMSGRGDKDIPQVAEIFGTKI
HHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
TIAPEPNSFRTGPDERGRFGIFGGRFVAETLMPLILELEAAYETAKKDPAFQAEMESYG
CCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
THYIGRPSPLYYAERLTEHLRAKAPAGQGAKVYFKREELNHTGSHKVNNVLGQILLARRM
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHC
GKPRIIAETGAGQHGVATATLCARFGLKCVVYMGAVDVARQAPNVFRMKMLGAEVVPVES
CCCEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHCCEEEECCC
GTKTLKDAMNEALRDWVTNVADTFYCIGTVAGPHPYPAMVRDFQSVIGRETREQMIAQEG
CHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHC
RIPDSLVACIGGGSNAMGLFHPFLDDREVEIFGVEAAGHGVQSGLHAASLTGGKPGVLHG
CCCHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEEECCCCHHHHCCHHHHCCCCCCCEEEC
NRTYLLMDGDGQIADAHSISAGLDYPGIGPEHAWLHEMGRVTYLSATDSETLEAFKLCSM
CEEEEEECCCCCEECCHHCCCCCCCCCCCCCHHHHHHCCCEEEEECCCHHHHHHHHHHHH
LEGIIPALEPAHALSKVLELAPQRPAEHLMVMNMSGRGDKDIPQVAEIFGTKI
HHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA