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The map label for this gene is rpfG [H]

Identifier: 170748031

GI number: 170748031

Start: 1718408

End: 1719871

Strand: Reverse

Name: rpfG [H]

Synonym: Mrad2831_1613

Alternate gene names: 170748031

Gene position: 1719871-1718408 (Counterclockwise)

Preceding gene: 170748033

Following gene: 170748030

Centisome position: 28.3

GC content: 73.09

Gene sequence:

>1464_bases
ATGGACGCGCTCCTCGTCGACGACAGCGCCACCATGCGGATGCGGCTGCGCCTCCTCCTGGAGGCCAAGCCCGACGTGTC
CGTCACCGATCACGGCGACCCGGTCGCCGCCATGGTCGAGGCGCGGACGCGGGCCTTCGACCTCGTCCTGGTCGACTGCC
ACATGCCCGGCATCGACGGGATCGCCTTCATCCGGCAGATGCGGACCATCCCGCACTACGCGCAGGTGCCCATCGTGATG
GTCACCAGCGACATCTCGGACGCGGTGCGGCTCGCCGCCCTGGAGGCCGGGGCGACCGATTTCCTCGACAAGTCCATGCA
GGGCGTCGAGCTCACCGTCCGCCTGCGCAACCTGATCCGGCTCGCCCGGGCGGTGCGGCGGCTGGCCGAGCAGGCGGCCT
GGCTCGACGGCGAGGTGGAGGCCGCCCTGCGCCACATGCGCGAGCGCGAGGAGGAGATCATCTTCCGCCTCGCGCTGGCG
GTCGAGTACCGCGACAACGACACCGGCGATCACACGTGGCGGGTGGCCCGCTACAGCCAGATCGTGGCCGAGGGCCTCGG
GCTGGCGCCGGAGATCTGCCGCAACCTCTACCTCGCGGCGCCGCTGCACGATGTCGGCAAGGTCGGCATTCCGGACGGCG
TCCTGCTGAAGCCCGGCCGGCTCGACCGGGACGAGTTCGACCTCGTCAAGACGCATCCGGCGATCGGTCGGCGCATCCTG
GGCGGAAGCGCCTCCGAGCTGATCCGGCTGGCCGCGGAGATCGCCGAGTTCCACCACGAGAAGTGGGACGGCGGCGGCTA
CCCGCACGGGCTCGCCGGCGCCGCCATCCCCCTGTCGGCCCGGATCGTCGCGGTGGCGGACGTGTTCGACGCCCTGACCA
CGGCGCGGCCCTACAAGGACGCGATGTCCTTCGACGCGGCCCTGGACTGCATCCGCGCCGAGAGCGGCCGCCATTTCGAC
CCGGACTGCGTGGCGGCGTTCTGCGCGCGCTTCCCGGACATCCGCGTCGCCGGCGGGCAGAACCGGGCGGCGGTGCGGCC
GAGCGCCGGGCCGGTCACGGAACCCTGGGCCCGGGTCCCGACCCTGCTTCGCGAGGTCACCCCGGCCGCGCCCGTCCCGA
CGGGGTGGCGCGCCGACGCCCTCGACACGCTCCTGAGACCGTCCCCGGAATCGTCCCCGGCACCGTCGGCGAACCCGGAC
GCGTTTGCGCCGTTGATCCCCGACCCGCACGGATCGCGGACGGAGCCGGACCATGACCCCGACCTGGAAGACGCTGCCGG
AGATCGCCCTGGCGCACGGGATCTCCCTGGAGGATGCCCAGCGCCTCGTGGACGGGGCGAACTGCCCGAAGGTGTTCCGC
GCGGACGGCACCGTCTACCTCATCTGAGCCCGTCCCGTCCGGGTGCGCCCGTCGCGGGTGCGTCCCACGCGGGTGCGCCC
CGCGAGTCCGTGCCGGGCGGATGA

Upstream 100 bases:

>100_bases
TCCCTGTGTCGACATACTTGGCCCGGCGGCCGCCCCGGGACGGCCTGCGGCGTCCCGGCTCCGGGCCGTCCGGGCGGGCG
CGCCGACCCGGGAGCCGCTG

Downstream 100 bases:

>100_bases
CGCCGGTCCCGGCGGGCCCTAACCTGCCCGCATGCGCATCGTCCACACGCCCGATTCGAGCCGGCACGACCCCGAACGCT
ACATGCGGCGCGGCGCGGCG

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MDALLVDDSATMRMRLRLLLEAKPDVSVTDHGDPVAAMVEARTRAFDLVLVDCHMPGIDGIAFIRQMRTIPHYAQVPIVM
VTSDISDAVRLAALEAGATDFLDKSMQGVELTVRLRNLIRLARAVRRLAEQAAWLDGEVEAALRHMREREEEIIFRLALA
VEYRDNDTGDHTWRVARYSQIVAEGLGLAPEICRNLYLAAPLHDVGKVGIPDGVLLKPGRLDRDEFDLVKTHPAIGRRIL
GGSASELIRLAAEIAEFHHEKWDGGGYPHGLAGAAIPLSARIVAVADVFDALTTARPYKDAMSFDAALDCIRAESGRHFD
PDCVAAFCARFPDIRVAGGQNRAAVRPSAGPVTEPWARVPTLLREVTPAAPVPTGWRADALDTLLRPSPESSPAPSANPD
AFAPLIPDPHGSRTEPDHDPDLEDAAGDRPGARDLPGGCPAPRGRGELPEGVPRGRHRLPHLSPSRPGAPVAGASHAGAP
RESVPGG

Sequences:

>Translated_487_residues
MDALLVDDSATMRMRLRLLLEAKPDVSVTDHGDPVAAMVEARTRAFDLVLVDCHMPGIDGIAFIRQMRTIPHYAQVPIVM
VTSDISDAVRLAALEAGATDFLDKSMQGVELTVRLRNLIRLARAVRRLAEQAAWLDGEVEAALRHMREREEEIIFRLALA
VEYRDNDTGDHTWRVARYSQIVAEGLGLAPEICRNLYLAAPLHDVGKVGIPDGVLLKPGRLDRDEFDLVKTHPAIGRRIL
GGSASELIRLAAEIAEFHHEKWDGGGYPHGLAGAAIPLSARIVAVADVFDALTTARPYKDAMSFDAALDCIRAESGRHFD
PDCVAAFCARFPDIRVAGGQNRAAVRPSAGPVTEPWARVPTLLREVTPAAPVPTGWRADALDTLLRPSPESSPAPSANPD
AFAPLIPDPHGSRTEPDHDPDLEDAAGDRPGARDLPGGCPAPRGRGELPEGVPRGRHRLPHLSPSRPGAPVAGASHAGAP
RESVPGG
>Mature_487_residues
MDALLVDDSATMRMRLRLLLEAKPDVSVTDHGDPVAAMVEARTRAFDLVLVDCHMPGIDGIAFIRQMRTIPHYAQVPIVM
VTSDISDAVRLAALEAGATDFLDKSMQGVELTVRLRNLIRLARAVRRLAEQAAWLDGEVEAALRHMREREEEIIFRLALA
VEYRDNDTGDHTWRVARYSQIVAEGLGLAPEICRNLYLAAPLHDVGKVGIPDGVLLKPGRLDRDEFDLVKTHPAIGRRIL
GGSASELIRLAAEIAEFHHEKWDGGGYPHGLAGAAIPLSARIVAVADVFDALTTARPYKDAMSFDAALDCIRAESGRHFD
PDCVAAFCARFPDIRVAGGQNRAAVRPSAGPVTEPWARVPTLLREVTPAAPVPTGWRADALDTLLRPSPESSPAPSANPD
AFAPLIPDPHGSRTEPDHDPDLEDAAGDRPGARDLPGGCPAPRGRGELPEGVPRGRHRLPHLSPSRPGAPVAGASHAGAP
RESVPGG

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=107, Percent_Identity=35.5140186915888, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786911, Length=152, Percent_Identity=32.2368421052632, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 52387; Mature: 52387

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALLVDDSATMRMRLRLLLEAKPDVSVTDHGDPVAAMVEARTRAFDLVLVDCHMPGIDG
CCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHEEEEEEEECCCCCCHH
IAFIRQMRTIPHYAQVPIVMVTSDISDAVRLAALEAGATDFLDKSMQGVELTVRLRNLIR
HHHHHHHHHCCCHHCCCEEEEECCHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHH
LARAVRRLAEQAAWLDGEVEAALRHMREREEEIIFRLALAVEYRDNDTGDHTWRVARYSQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHH
IVAEGLGLAPEICRNLYLAAPLHDVGKVGIPDGVLLKPGRLDRDEFDLVKTHPAIGRRIL
HHHHHCCCCHHHHHHHEECCCHHHHCCCCCCCCEEECCCCCCCHHHHHHHCCHHHHHHHH
GGSASELIRLAAEIAEFHHEKWDGGGYPHGLAGAAIPLSARIVAVADVFDALTTARPYKD
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHH
AMSFDAALDCIRAESGRHFDPDCVAAFCARFPDIRVAGGQNRAAVRPSAGPVTEPWARVP
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEECCCCCCEECCCCCCCCCHHHHHH
TLLREVTPAAPVPTGWRADALDTLLRPSPESSPAPSANPDAFAPLIPDPHGSRTEPDHDP
HHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DLEDAAGDRPGARDLPGGCPAPRGRGELPEGVPRGRHRLPHLSPSRPGAPVAGASHAGAP
CCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCC
RESVPGG
CCCCCCC
>Mature Secondary Structure
MDALLVDDSATMRMRLRLLLEAKPDVSVTDHGDPVAAMVEARTRAFDLVLVDCHMPGIDG
CCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHEEEEEEEECCCCCCHH
IAFIRQMRTIPHYAQVPIVMVTSDISDAVRLAALEAGATDFLDKSMQGVELTVRLRNLIR
HHHHHHHHHCCCHHCCCEEEEECCHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHH
LARAVRRLAEQAAWLDGEVEAALRHMREREEEIIFRLALAVEYRDNDTGDHTWRVARYSQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHH
IVAEGLGLAPEICRNLYLAAPLHDVGKVGIPDGVLLKPGRLDRDEFDLVKTHPAIGRRIL
HHHHHCCCCHHHHHHHEECCCHHHHCCCCCCCCEEECCCCCCCHHHHHHHCCHHHHHHHH
GGSASELIRLAAEIAEFHHEKWDGGGYPHGLAGAAIPLSARIVAVADVFDALTTARPYKD
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHH
AMSFDAALDCIRAESGRHFDPDCVAAFCARFPDIRVAGGQNRAAVRPSAGPVTEPWARVP
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEECCCCCCEECCCCCCCCCHHHHHH
TLLREVTPAAPVPTGWRADALDTLLRPSPESSPAPSANPDAFAPLIPDPHGSRTEPDHDP
HHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DLEDAAGDRPGARDLPGGCPAPRGRGELPEGVPRGRHRLPHLSPSRPGAPVAGASHAGAP
CCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCC
RESVPGG
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]