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The map label for this gene is emrA [H]

Identifier: 170748026

GI number: 170748026

Start: 1712730

End: 1713974

Strand: Reverse

Name: emrA [H]

Synonym: Mrad2831_1608

Alternate gene names: 170748026

Gene position: 1713974-1712730 (Counterclockwise)

Preceding gene: 170748029

Following gene: 170748025

Centisome position: 28.2

GC content: 74.3

Gene sequence:

>1245_bases
ATGGACGCACGTCGCAGCGACAGCTTCAAGAGCGCCGACCCGGTCGCGGGCGCCCCGGTCTCCCCGCCGGCGACCGCGGC
TGTCACGCCCCCGGTCTCCACGCCGGTCCCGGCCCCCGTCTCGCCCCCGGCGCCGCGCCGCCGCCGCGCGCGGACCGTGC
TGCCCCTCCTCGGCCTGCTCGCGCTCGGCGGGGCCGGGACCTACGGCTGGCACTGGTGGACCGTGGGGCGCTTCCTCGAG
GTGACCGACGACGCCTATCTCCAGTCCGACAAGGTCACGGTGGCGCCGCGCATCGCCGGCACCGTCGCGGCGGTGCTGGT
CGGCGACAACCAGCCGGTGAAGGCCGGCGAGGTGATCGCGCGCCTCGACGACCGGTCCTACCGGGTCCAGCTCAAGCAGG
CCGAGGCCGAGGTCGAGAAGGACCGGGCGCAGATCCTGGGCGTCGCCTCGGCGATCGTCCAGCAGCGGGCGCAGGTCGCC
TCGTCGCGGGCCGACCTCGCCAACGCCGAGGCCGCCCTGACCTTCGCGCAGCAGGAATACACCCGCTACCAGAACCTGCT
GCAGACCGGCTCGGGCACGGTCCAGCGCCAGCAGCAGGCGGATGCCGACCTCCGCCAGCGCCACGCCGCCCGCGACAAGG
CCGCCGCCTCGCTCGACGCCGCGCAGAAGCAGATCGACAGCCTGAAGGCCCTCGAGGCGAGCACCCGGGCGAGCCTGGAG
GGCGCGCAGGCGAAGGTCGAGGGCGTGAGGCTCGACCTCAGCTACACCACCATCGTGGCACCGATCGACGGCGTGGCCGG
CGACCGGGCGCTGCGGATCGGGCAGGTCGTCTCGGCCGGCACCGGGCTCCTGACCCTCGTGCCGATGGGCCGCGACATCT
ACCTCGTGGCGAACTTCAAGGAGACCCAGACCGGCCGCATGGTCGAGGGGCAGCACGTGACCTTCACGGTCGACGCCTTC
GGCGATCATGAATTCCAGGGCCGGGTCGAGAGCTTCTCGCCGGGCACCGGCTCGCAGTTCGCCCTGCTGCCGCCCGAGAA
CGCCACCGGCAACTTCACCAAGGTGGTGCAGCGCGTGCCCGTGCGCGTCGCCCTCGACCCGTCGGACCCGCTCATCGCCC
GCCTGCGTCCGGGCCTGTCGGTGGAGGCCACGGTCCACACCGCCGACCCGGTGGAGCCGGTGGATCCCGCCCGCCGCCGG
CCGAGGCCCGCCCCGGCGCTGGTCAGCGAGGCCCTGCCGCGATGA

Upstream 100 bases:

>100_bases
TGACCGTCACCCCGCGATCCTCCCTGCTCACCGGCTTGCTCCACGGCGCCGTTCGTCCTATCAAAACGATACCGTACCGT
TTTGATTCTGGCCAGACCCG

Downstream 100 bases:

>100_bases
GCCCGGCCGCGATGAGCCCGGCCCCGATGAGCCCGGTCCCGGCCGTGGCGGTGGCCGAGGCGAAGGCGAGCGCCCGGGAC
TGGCTCGCCGTGTTCGGCGC

Product: secretion protein HlyD family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MDARRSDSFKSADPVAGAPVSPPATAAVTPPVSTPVPAPVSPPAPRRRRARTVLPLLGLLALGGAGTYGWHWWTVGRFLE
VTDDAYLQSDKVTVAPRIAGTVAAVLVGDNQPVKAGEVIARLDDRSYRVQLKQAEAEVEKDRAQILGVASAIVQQRAQVA
SSRADLANAEAALTFAQQEYTRYQNLLQTGSGTVQRQQQADADLRQRHAARDKAAASLDAAQKQIDSLKALEASTRASLE
GAQAKVEGVRLDLSYTTIVAPIDGVAGDRALRIGQVVSAGTGLLTLVPMGRDIYLVANFKETQTGRMVEGQHVTFTVDAF
GDHEFQGRVESFSPGTGSQFALLPPENATGNFTKVVQRVPVRVALDPSDPLIARLRPGLSVEATVHTADPVEPVDPARRR
PRPAPALVSEALPR

Sequences:

>Translated_414_residues
MDARRSDSFKSADPVAGAPVSPPATAAVTPPVSTPVPAPVSPPAPRRRRARTVLPLLGLLALGGAGTYGWHWWTVGRFLE
VTDDAYLQSDKVTVAPRIAGTVAAVLVGDNQPVKAGEVIARLDDRSYRVQLKQAEAEVEKDRAQILGVASAIVQQRAQVA
SSRADLANAEAALTFAQQEYTRYQNLLQTGSGTVQRQQQADADLRQRHAARDKAAASLDAAQKQIDSLKALEASTRASLE
GAQAKVEGVRLDLSYTTIVAPIDGVAGDRALRIGQVVSAGTGLLTLVPMGRDIYLVANFKETQTGRMVEGQHVTFTVDAF
GDHEFQGRVESFSPGTGSQFALLPPENATGNFTKVVQRVPVRVALDPSDPLIARLRPGLSVEATVHTADPVEPVDPARRR
PRPAPALVSEALPR
>Mature_414_residues
MDARRSDSFKSADPVAGAPVSPPATAAVTPPVSTPVPAPVSPPAPRRRRARTVLPLLGLLALGGAGTYGWHWWTVGRFLE
VTDDAYLQSDKVTVAPRIAGTVAAVLVGDNQPVKAGEVIARLDDRSYRVQLKQAEAEVEKDRAQILGVASAIVQQRAQVA
SSRADLANAEAALTFAQQEYTRYQNLLQTGSGTVQRQQQADADLRQRHAARDKAAASLDAAQKQIDSLKALEASTRASLE
GAQAKVEGVRLDLSYTTIVAPIDGVAGDRALRIGQVVSAGTGLLTLVPMGRDIYLVANFKETQTGRMVEGQHVTFTVDAF
GDHEFQGRVESFSPGTGSQFALLPPENATGNFTKVVQRVPVRVALDPSDPLIARLRPGLSVEATVHTADPVEPVDPARRR
PRPAPALVSEALPR

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=359, Percent_Identity=32.3119777158774, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788711, Length=350, Percent_Identity=31.4285714285714, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1790519, Length=301, Percent_Identity=30.8970099667774, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI87081951, Length=304, Percent_Identity=23.6842105263158, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789900, Length=263, Percent_Identity=25.8555133079848, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787013, Length=272, Percent_Identity=27.5735294117647, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87082036, Length=225, Percent_Identity=25.7777777777778, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44094; Mature: 44094

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDARRSDSFKSADPVAGAPVSPPATAAVTPPVSTPVPAPVSPPAPRRRRARTVLPLLGLL
CCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ALGGAGTYGWHWWTVGRFLEVTDDAYLQSDKVTVAPRIAGTVAAVLVGDNQPVKAGEVIA
HHCCCCCCCEEEEECCCEEEECCCHHCCCCCEEECCHHHCEEEEEEECCCCCCCHHHHHH
RLDDRSYRVQLKQAEAEVEKDRAQILGVASAIVQQRAQVASSRADLANAEAALTFAQQEY
HCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
TRYQNLLQTGSGTVQRQQQADADLRQRHAARDKAAASLDAAQKQIDSLKALEASTRASLE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GAQAKVEGVRLDLSYTTIVAPIDGVAGDRALRIGQVVSAGTGLLTLVPMGRDIYLVANFK
CCCEEEEEEEEEEEEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCEEEEEEECC
ETQTGRMVEGQHVTFTVDAFGDHEFQGRVESFSPGTGSQFALLPPENATGNFTKVVQRVP
CCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCC
VRVALDPSDPLIARLRPGLSVEATVHTADPVEPVDPARRRPRPAPALVSEALPR
EEEEECCCCCHHHHCCCCCEEEEEEECCCCCCCCCHHHCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MDARRSDSFKSADPVAGAPVSPPATAAVTPPVSTPVPAPVSPPAPRRRRARTVLPLLGLL
CCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ALGGAGTYGWHWWTVGRFLEVTDDAYLQSDKVTVAPRIAGTVAAVLVGDNQPVKAGEVIA
HHCCCCCCCEEEEECCCEEEECCCHHCCCCCEEECCHHHCEEEEEEECCCCCCCHHHHHH
RLDDRSYRVQLKQAEAEVEKDRAQILGVASAIVQQRAQVASSRADLANAEAALTFAQQEY
HCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
TRYQNLLQTGSGTVQRQQQADADLRQRHAARDKAAASLDAAQKQIDSLKALEASTRASLE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GAQAKVEGVRLDLSYTTIVAPIDGVAGDRALRIGQVVSAGTGLLTLVPMGRDIYLVANFK
CCCEEEEEEEEEEEEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCEEEEEEECC
ETQTGRMVEGQHVTFTVDAFGDHEFQGRVESFSPGTGSQFALLPPENATGNFTKVVQRVP
CCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCC
VRVALDPSDPLIARLRPGLSVEATVHTADPVEPVDPARRRPRPAPALVSEALPR
EEEEECCCCCHHHHCCCCCEEEEEEECCCCCCCCCHHHCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]