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The map label for this gene is emrB [H]

Identifier: 170748025

GI number: 170748025

Start: 1711126

End: 1712733

Strand: Reverse

Name: emrB [H]

Synonym: Mrad2831_1607

Alternate gene names: 170748025

Gene position: 1712733-1711126 (Counterclockwise)

Preceding gene: 170748026

Following gene: 170748022

Centisome position: 28.18

GC content: 70.34

Gene sequence:

>1608_bases
ATGAGCCCGGCCGCGATGAGCCCGGCCCCGATGAGCCCGGTCCCGGCCGTGGCGGTGGCCGAGGCGAAGGCGAGCGCCCG
GGACTGGCTCGCCGTGTTCGGCGCGATCCTCGGCGCCTTCACGGCGATCCTCGACATCCAGATCACCAACGCCTCGCTCG
CCGACATCCAGGGGGCGCTCGGCGCCTCCACCGAGGAGGGGAGCTGGATCTCGACCGCCTACCTGATGGCCGAGATCATC
GTGATCCCGCTCACCGGCTGGCTGTCCTCGGTGATCGGCCTGCGCCGCTACCTCGCGGTGAACACCATCCTGTTCACCGC
CTTCTCGCTGGCCTGCGCGCTCTCGACGTCCTTGAGCCAGATGATCCTGTTCCGCGCCGGGCAGGGCTTCACGGGCGGCG
TGCTGATCCCCACCGCGATCGTGATCGTGCGCACGCGGCTGCCCAAGAGCCAGCAGGCGGTCGGCATCGCCATGTTCGGG
CTGACCGCCACCTTCGCGCCGGCCATCGGCCCGACGGTCGGCGGCTGGCTCACCGACAACCTGTCCTGGCACTACATCTT
CTACCTGAACCTGATCTTCGGTCCGATCGCGGCCGCGATCCAGCTCGGCGCCTTCGAGAAGGCGCCCGCCCGCTGGGGCG
AGCTGCTCAAGGGCGACTGGATCGGCATCGGCCTGATGGCGACGGGCCTGCCGGCCCTGACCTTCGTGCTGGAGGAGGGC
CAGCGCAAGGACTGGTTCGGCTCGCCCGTGATCGTCGAGGCGAGCTGGATCGCGGCGGCCGGCATCGCCGGGTTCATCGT
CCGCGAGCTGACGGCGGCGCGGCCGTTCATCAACCTGCGGGTGCTGGCCAACCGCTCGGTCGGCGGCGCCTGCGTGCTGA
TGACCGTGCTGGGCGCGGTCTCGTACGGGTCGATCTACATCATCCCGGTCTACTGCGCGCAGATCCAGGGCTACAACGCC
CAGCAGATCGGCTACGTGGTGATGTGGTCGGGCCTGCCGCAGCTGCTGCTGTTCCCGATGATGCCGCTGATCATGCGCGC
CGTGGACGCGCGGCTGCTCGTGGTCACCGGCACGCTGTTCTTCATCGCGAGCTGCTGGATCAATGTCGGCCTGACCCACG
ACGTCGGCGCCGACCAGCTGATCCTGCCGCAGATCATGCGCGCCATCGGCCAGCCGCTGTTCACGATCCCGCTCTCGCAG
CTCTCGACGGCCGGCCTCGCGCCGCGCGACACCGCGGACGCCTCGGCGCTGTCGAACATGATGCGCAACCTCGGCGGCTC
GATCGGCATCGCCATGCTGTCGACCATGATCGATCGGCGGGAGCACTTCCATTTCTCGATGCTGGCCGAGGCGATCACCG
TCAACGCCACCCGCACCCAGGACCGGCTCGCGGCGCTCGCCGCCGGGATGCACGCCCGGATCGCCGACCCCCTCGCCGCC
AAGGGCGTCGCGCTCGGCCAGCTCGCCGCGCAGGTCCGCCGCGAGGCCTACGTGATGGCCTACGCGGACGGGTTCTGGCT
GGTGGGGGTGAGCCTCGTGGCGAGCCTCGCGGTGGTGGCGGTCCTCAAGAAGCCGCAGCGCGCCGCCGGACCCGTGGAGG
CGCACTGA

Upstream 100 bases:

>100_bases
TGGAGGCCACGGTCCACACCGCCGACCCGGTGGAGCCGGTGGATCCCGCCCGCCGCCGGCCGAGGCCCGCCCCGGCGCTG
GTCAGCGAGGCCCTGCCGCG

Downstream 100 bases:

>100_bases
CCGGCCCGGGGCCTACTTCCCCGGCAGGTTGTGGACGATCGGGAAGAACGTGTCCTTCCAGTCCTTGGGCTCGGCCTTGA
TGGTCTTCATCGCGTAGAAG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MSPAAMSPAPMSPVPAVAVAEAKASARDWLAVFGAILGAFTAILDIQITNASLADIQGALGASTEEGSWISTAYLMAEII
VIPLTGWLSSVIGLRRYLAVNTILFTAFSLACALSTSLSQMILFRAGQGFTGGVLIPTAIVIVRTRLPKSQQAVGIAMFG
LTATFAPAIGPTVGGWLTDNLSWHYIFYLNLIFGPIAAAIQLGAFEKAPARWGELLKGDWIGIGLMATGLPALTFVLEEG
QRKDWFGSPVIVEASWIAAAGIAGFIVRELTAARPFINLRVLANRSVGGACVLMTVLGAVSYGSIYIIPVYCAQIQGYNA
QQIGYVVMWSGLPQLLLFPMMPLIMRAVDARLLVVTGTLFFIASCWINVGLTHDVGADQLILPQIMRAIGQPLFTIPLSQ
LSTAGLAPRDTADASALSNMMRNLGGSIGIAMLSTMIDRREHFHFSMLAEAITVNATRTQDRLAALAAGMHARIADPLAA
KGVALGQLAAQVRREAYVMAYADGFWLVGVSLVASLAVVAVLKKPQRAAGPVEAH

Sequences:

>Translated_535_residues
MSPAAMSPAPMSPVPAVAVAEAKASARDWLAVFGAILGAFTAILDIQITNASLADIQGALGASTEEGSWISTAYLMAEII
VIPLTGWLSSVIGLRRYLAVNTILFTAFSLACALSTSLSQMILFRAGQGFTGGVLIPTAIVIVRTRLPKSQQAVGIAMFG
LTATFAPAIGPTVGGWLTDNLSWHYIFYLNLIFGPIAAAIQLGAFEKAPARWGELLKGDWIGIGLMATGLPALTFVLEEG
QRKDWFGSPVIVEASWIAAAGIAGFIVRELTAARPFINLRVLANRSVGGACVLMTVLGAVSYGSIYIIPVYCAQIQGYNA
QQIGYVVMWSGLPQLLLFPMMPLIMRAVDARLLVVTGTLFFIASCWINVGLTHDVGADQLILPQIMRAIGQPLFTIPLSQ
LSTAGLAPRDTADASALSNMMRNLGGSIGIAMLSTMIDRREHFHFSMLAEAITVNATRTQDRLAALAAGMHARIADPLAA
KGVALGQLAAQVRREAYVMAYADGFWLVGVSLVASLAVVAVLKKPQRAAGPVEAH
>Mature_534_residues
SPAAMSPAPMSPVPAVAVAEAKASARDWLAVFGAILGAFTAILDIQITNASLADIQGALGASTEEGSWISTAYLMAEIIV
IPLTGWLSSVIGLRRYLAVNTILFTAFSLACALSTSLSQMILFRAGQGFTGGVLIPTAIVIVRTRLPKSQQAVGIAMFGL
TATFAPAIGPTVGGWLTDNLSWHYIFYLNLIFGPIAAAIQLGAFEKAPARWGELLKGDWIGIGLMATGLPALTFVLEEGQ
RKDWFGSPVIVEASWIAAAGIAGFIVRELTAARPFINLRVLANRSVGGACVLMTVLGAVSYGSIYIIPVYCAQIQGYNAQ
QIGYVVMWSGLPQLLLFPMMPLIMRAVDARLLVVTGTLFFIASCWINVGLTHDVGADQLILPQIMRAIGQPLFTIPLSQL
STAGLAPRDTADASALSNMMRNLGGSIGIAMLSTMIDRREHFHFSMLAEAITVNATRTQDRLAALAAGMHARIADPLAAK
GVALGQLAAQVRREAYVMAYADGFWLVGVSLVASLAVVAVLKKPQRAAGPVEAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=493, Percent_Identity=29.2089249492901, Blast_Score=224, Evalue=2e-59,
Organism=Escherichia coli, GI1788710, Length=475, Percent_Identity=28.8421052631579, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1790195, Length=438, Percent_Identity=25.3424657534247, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI87081983, Length=425, Percent_Identity=25.8823529411765, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788392, Length=216, Percent_Identity=30.0925925925926, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325455, Length=132, Percent_Identity=29.5454545454545, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=153, Percent_Identity=28.1045751633987, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56581; Mature: 56450

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPAAMSPAPMSPVPAVAVAEAKASARDWLAVFGAILGAFTAILDIQITNASLADIQGAL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHC
GASTEEGSWISTAYLMAEIIVIPLTGWLSSVIGLRRYLAVNTILFTAFSLACALSTSLSQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MILFRAGQGFTGGVLIPTAIVIVRTRLPKSQQAVGIAMFGLTATFAPAIGPTVGGWLTDN
HHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
LSWHYIFYLNLIFGPIAAAIQLGAFEKAPARWGELLKGDWIGIGLMATGLPALTFVLEEG
CCEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEHHHHHCHHHHHHHHHCC
QRKDWFGSPVIVEASWIAAAGIAGFIVRELTAARPFINLRVLANRSVGGACVLMTVLGAV
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHH
SYGSIYIIPVYCAQIQGYNAQQIGYVVMWSGLPQLLLFPMMPLIMRAVDARLLVVTGTLF
CCCCEEEEEEEEEHHCCCCCHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEHHHHHH
FIASCWINVGLTHDVGADQLILPQIMRAIGQPLFTIPLSQLSTAGLAPRDTADASALSNM
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEEHHHHHHCCCCCCCCCHHHHHHHH
MRNLGGSIGIAMLSTMIDRREHFHFSMLAEAITVNATRTQDRLAALAAGMHARIADPLAA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHCCCHHH
KGVALGQLAAQVRREAYVMAYADGFWLVGVSLVASLAVVAVLKKPQRAAGPVEAH
CCHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SPAAMSPAPMSPVPAVAVAEAKASARDWLAVFGAILGAFTAILDIQITNASLADIQGAL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHC
GASTEEGSWISTAYLMAEIIVIPLTGWLSSVIGLRRYLAVNTILFTAFSLACALSTSLSQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MILFRAGQGFTGGVLIPTAIVIVRTRLPKSQQAVGIAMFGLTATFAPAIGPTVGGWLTDN
HHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
LSWHYIFYLNLIFGPIAAAIQLGAFEKAPARWGELLKGDWIGIGLMATGLPALTFVLEEG
CCEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEHHHHHCHHHHHHHHHCC
QRKDWFGSPVIVEASWIAAAGIAGFIVRELTAARPFINLRVLANRSVGGACVLMTVLGAV
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHH
SYGSIYIIPVYCAQIQGYNAQQIGYVVMWSGLPQLLLFPMMPLIMRAVDARLLVVTGTLF
CCCCEEEEEEEEEHHCCCCCHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEHHHHHH
FIASCWINVGLTHDVGADQLILPQIMRAIGQPLFTIPLSQLSTAGLAPRDTADASALSNM
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEEHHHHHHCCCCCCCCCHHHHHHHH
MRNLGGSIGIAMLSTMIDRREHFHFSMLAEAITVNATRTQDRLAALAAGMHARIADPLAA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHCCCHHH
KGVALGQLAAQVRREAYVMAYADGFWLVGVSLVASLAVVAVLKKPQRAAGPVEAH
CCHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]