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The map label for this gene is puuR [H]

Identifier: 170747509

GI number: 170747509

Start: 1134443

End: 1135003

Strand: Reverse

Name: puuR [H]

Synonym: Mrad2831_1077

Alternate gene names: 170747509

Gene position: 1135003-1134443 (Counterclockwise)

Preceding gene: 170747512

Following gene: 170747508

Centisome position: 18.67

GC content: 72.37

Gene sequence:

>561_bases
ATGAGCATCGATGTCGGCGCGCGCCTGCGCTTCGTTCGGGCCCGCCACGGCCTGTCGCAGCGGACGCTCGCCAAGCGCGC
CGGGGTGACCAATTCCGCGATCTCGCTGATCGAGGCCAACCGGGTGAACCCGTCGGTGGGCGCCCTGAAGCGGATCCTCG
ACGGCGTGCCGATCGGCATGGCCGAGTTCTTCGCCCTGGAGCCGGAGCCGGAGCGCAAGGCGTTCTTCGCCGCCGAGGAG
CTCACCGAGATCGGCCGCCGGGCCGCCAAGGGGGCGATCTCGTACCGGCAGGTCGGCGACAGCCTGTTCGGGCGCAAGCT
CCAGATCCTCAAGGAGCGCTACGAGCCCGGCGCCGATACCGGCCGGGTGCCGCTCTCGCACGAGGGCGAGGAGGGCGGGA
TCGTGCTCTCCGGGCGCCTGGAGGTGACGGTGGACGCCGAGCGCCGGATCCTCGGCCCCGGCGACGCCTACAGCTTCGAC
AGCCGCCGCCCGCACCGGTTCCGCTGCGTCGGGCCCAATCCCTGCGAGGTGGTGAGCGCCTGCACGCCGCCGAGCTTCTG
A

Upstream 100 bases:

>100_bases
GCAACAAGTCCTTTTCTGGTCGCCGCCAACCCCTTGCAGGATCGGGGCCGCGGGTGGCACCGTTGCGGGATCCGCAACAC
CTGAAACGGGCCTGAGCCGG

Downstream 100 bases:

>100_bases
CCGCGCCGATCCCATGCCGAGCGACAGCGTCGTCCTCCTGCACGGTCTGGGGCGAAGAGCCGCCTCGCTCGCCCGGATGC
GCTCGGCCCTGACGGCGGAG

Product: XRE family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MSIDVGARLRFVRARHGLSQRTLAKRAGVTNSAISLIEANRVNPSVGALKRILDGVPIGMAEFFALEPEPERKAFFAAEE
LTEIGRRAAKGAISYRQVGDSLFGRKLQILKERYEPGADTGRVPLSHEGEEGGIVLSGRLEVTVDAERRILGPGDAYSFD
SRRPHRFRCVGPNPCEVVSACTPPSF

Sequences:

>Translated_186_residues
MSIDVGARLRFVRARHGLSQRTLAKRAGVTNSAISLIEANRVNPSVGALKRILDGVPIGMAEFFALEPEPERKAFFAAEE
LTEIGRRAAKGAISYRQVGDSLFGRKLQILKERYEPGADTGRVPLSHEGEEGGIVLSGRLEVTVDAERRILGPGDAYSFD
SRRPHRFRCVGPNPCEVVSACTPPSF
>Mature_185_residues
SIDVGARLRFVRARHGLSQRTLAKRAGVTNSAISLIEANRVNPSVGALKRILDGVPIGMAEFFALEPEPERKAFFAAEEL
TEIGRRAAKGAISYRQVGDSLFGRKLQILKERYEPGADTGRVPLSHEGEEGGIVLSGRLEVTVDAERRILGPGDAYSFDS
RRPHRFRCVGPNPCEVVSACTPPSF

Specific function: Represses puuD [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=183, Percent_Identity=34.4262295081967, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 20288; Mature: 20157

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDVGARLRFVRARHGLSQRTLAKRAGVTNSAISLIEANRVNPSVGALKRILDGVPIGM
CCCCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCH
AEFFALEPEPERKAFFAAEELTEIGRRAAKGAISYRQVGDSLFGRKLQILKERYEPGADT
HHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GRVPLSHEGEEGGIVLSGRLEVTVDAERRILGPGDAYSFDSRRPHRFRCVGPNPCEVVSA
CCCCCCCCCCCCCEEEECEEEEEECCCCEEECCCCCCCCCCCCCCEEEECCCCHHHHHCC
CTPPSF
CCCCCC
>Mature Secondary Structure 
SIDVGARLRFVRARHGLSQRTLAKRAGVTNSAISLIEANRVNPSVGALKRILDGVPIGM
CCCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCH
AEFFALEPEPERKAFFAAEELTEIGRRAAKGAISYRQVGDSLFGRKLQILKERYEPGADT
HHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GRVPLSHEGEEGGIVLSGRLEVTVDAERRILGPGDAYSFDSRRPHRFRCVGPNPCEVVSA
CCCCCCCCCCCCCEEEECEEEEEECCCCEEECCCCCCCCCCCCCCEEEECCCCHHHHHCC
CTPPSF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]