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The map label for this gene is leuD

Identifier: 170747396

GI number: 170747396

Start: 1019187

End: 1019789

Strand: Reverse

Name: leuD

Synonym: Mrad2831_0963

Alternate gene names: 170747396

Gene position: 1019789-1019187 (Counterclockwise)

Preceding gene: 170747397

Following gene: 170747394

Centisome position: 16.78

GC content: 66.33

Gene sequence:

>603_bases
ATGGAAAAGTTCACCACCCTGGAGGGCGTCGCGGCGCCCCTGCGGACGATCAACGTCGATACCGACCGGATCATCCCGGC
GAAGTACCTGAAGACGATCCGGCGGACCGGGCTCGGCAAGAGCCTGTTCGCCGAGATGCGCTACCGCGAGGACGGCTCCG
AGAACCCGGACTTCATCCTCAACCAGCCGGCCTACCGGAACAGCAAGATCCTGGTGTGCGGGGACAATTTCGGCTGCGGC
TCGTCCCGGGAGCACGCGCCCTGGGCGCTGGCCGATTTCGGCATCCGCTGCGTGATCTCCACGAGCTTCGCCGACATCTT
CTTCAACAACTGCGCCAAGAACGGCATCCTGGCGATCGTCGTGTCGCCCGAGGACCTGGAGAAGCTGTTCGAGGACGCCG
AGCGCGGCGCGAACGCCACCCTGACGGTCGATCTGGCGGCGCAGACGATCCGGGGGCCGGACGGCGGCACCCTGCATTTC
GACGTCGACGAGGGCCGCAAGCACAACCTGCTGAACGGACTCGACGAGATCGGGCTGACCCTCGAGCGCGTCTCGTCCAT
CGACGCCTACGAGCAGAAGCTCGCCCAGCGCACGTGGGCCTGA

Upstream 100 bases:

>100_bases
GTCCCGGGCGACGGCGCCCGACCTCGCCTGACGCTTCCGGCCCGGGCCGGCGGCTGCTAAACCGCCCGGCAGAGAGACCA
CCGACACGAGCCGTCAGACG

Downstream 100 bases:

>100_bases
GCGGCGGTATCCGCGTCTCGACGGCGCTCGCGTCGCGCTGAGGCGACCGCCGGTCCGACCCGGATGCGGCCTCACCCGAG
CCGCATGGACAGTTCGACGT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MEKFTTLEGVAAPLRTINVDTDRIIPAKYLKTIRRTGLGKSLFAEMRYREDGSENPDFILNQPAYRNSKILVCGDNFGCG
SSREHAPWALADFGIRCVISTSFADIFFNNCAKNGILAIVVSPEDLEKLFEDAERGANATLTVDLAAQTIRGPDGGTLHF
DVDEGRKHNLLNGLDEIGLTLERVSSIDAYEQKLAQRTWA

Sequences:

>Translated_200_residues
MEKFTTLEGVAAPLRTINVDTDRIIPAKYLKTIRRTGLGKSLFAEMRYREDGSENPDFILNQPAYRNSKILVCGDNFGCG
SSREHAPWALADFGIRCVISTSFADIFFNNCAKNGILAIVVSPEDLEKLFEDAERGANATLTVDLAAQTIRGPDGGTLHF
DVDEGRKHNLLNGLDEIGLTLERVSSIDAYEQKLAQRTWA
>Mature_200_residues
MEKFTTLEGVAAPLRTINVDTDRIIPAKYLKTIRRTGLGKSLFAEMRYREDGSENPDFILNQPAYRNSKILVCGDNFGCG
SSREHAPWALADFGIRCVISTSFADIFFNNCAKNGILAIVVSPEDLEKLFEDAERGANATLTVDLAAQTIRGPDGGTLHF
DVDEGRKHNLLNGLDEIGLTLERVSSIDAYEQKLAQRTWA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=194, Percent_Identity=44.8453608247423, Blast_Score=178, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6321429, Length=198, Percent_Identity=52.020202020202, Blast_Score=194, Evalue=7e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_METRJ (B1LZS5)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001753656.1
- ProteinModelPortal:   B1LZS5
- SMR:   B1LZS5
- GeneID:   6136980
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_0963
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22111; Mature: 22111

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFTTLEGVAAPLRTINVDTDRIIPAKYLKTIRRTGLGKSLFAEMRYREDGSENPDFIL
CCCCCCHHHHHHHHEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEE
NQPAYRNSKILVCGDNFGCGSSREHAPWALADFGIRCVISTSFADIFFNNCAKNGILAIV
CCCCCCCCEEEEECCCCCCCCCCCCCCEEHHCCCEEEEEECHHHHHHHHHCCCCCEEEEE
VSPEDLEKLFEDAERGANATLTVDLAAQTIRGPDGGTLHFDVDEGRKHNLLNGLDEIGLT
ECHHHHHHHHHHHHCCCCCEEEEEEEHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
LERVSSIDAYEQKLAQRTWA
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKFTTLEGVAAPLRTINVDTDRIIPAKYLKTIRRTGLGKSLFAEMRYREDGSENPDFIL
CCCCCCHHHHHHHHEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEE
NQPAYRNSKILVCGDNFGCGSSREHAPWALADFGIRCVISTSFADIFFNNCAKNGILAIV
CCCCCCCCEEEEECCCCCCCCCCCCCCEEHHCCCEEEEEECHHHHHHHHHCCCCCEEEEE
VSPEDLEKLFEDAERGANATLTVDLAAQTIRGPDGGTLHFDVDEGRKHNLLNGLDEIGLT
ECHHHHHHHHHHHHCCCCCEEEEEEEHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
LERVSSIDAYEQKLAQRTWA
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA