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The map label for this gene is tolB [H]

Identifier: 170747178

GI number: 170747178

Start: 788652

End: 789989

Strand: Reverse

Name: tolB [H]

Synonym: Mrad2831_0744

Alternate gene names: 170747178

Gene position: 789989-788652 (Counterclockwise)

Preceding gene: 170747179

Following gene: 170747170

Centisome position: 13.0

GC content: 70.63

Gene sequence:

>1338_bases
ATGCTGCACCTGCCCTCCGTCGGGCGACTCCCGACGTCCTTCGCCGCGCGCCTGATCGCGGTCTTCACCCTGCTGACGGC
CGTCCTGGCCGGGCCCGCCCAGGCGCAGCTGCAGCTGCGCATCGGCGGCGGCAACTTCCAGCCGATGCCCATCGCCATCG
CGGATTTCGGCGGCGATCCGAGCCTCGCCGGCCTCGTCTCCGGCGTGGTCACCAACGACCTGCGCCGCTCCGGCTACTTC
ACGCCACTCGATCACGCCCGCTTCCCCGAGCAGCCGAACTTCGACGCCGCCCCGAACTTCGAGAAGTGGCGCGCCACCGG
CGTCCAGGGCCTCGTCACCGGCCGCGTCGTCCGCGAGGGCGGCCGGGTCAAGGTCGAGTTCCGGCTCTGGGACGTGACCA
ACGGCCAGCAGATGACCGGCCAGCAATACGGCACCGACGCCGCCAACGTCCGGCGCACCGGCCACCTGATCTCCGACGGG
GTCTACACCAAGATCACCGGCCTCGGGCCCTGGTTCGACAGCCGCATCGCCTTCGTGGACGAGACCGGCCCGAAGGAGAA
CCGCCGCAAGCGCCTGATGGTCATGGACCAGGACGGCGCCAATGTCCGGGCGATCACCACCGGCGAGATGTCGATCGTGG
CGCCGCGCTACTCGCCGGCCACCCAGGACATCGCCTTCATGGCCCAGGCCACCGGCCACCAGCCACGGGTGCAGATGATC
AACCTGGAGACCGGCTCCCGCCAGGCGCTCGGCAACGTCGATTCGATGAGCGCGAGCCCGCGCTTCTCGCCGGACGGCCG
GAAGATCGTGATGAGCGTCCAGCAGGGCGGCAACGCCGACATCGTGACGATGGACCTCGCCTCGAAGGCCCAGCGCTCGA
TCACCAACGGCATGGCGATCGACACCTCGCCGACCTACTCGCCGGACGGATCGCAGATCGCCTTCGAGTCCGACCGCGGC
GGCTCCCAGCAGGTCTACGTGATGGGGGCGGACGGCTCGAACCCGCACCGGATCACCTTCGGTCCCGGCTCGGCCTCGCA
GCCGGCCTTCTCGCCCCGCGGCGACCTGATCGCCTACACGCGCCAGCGCAACGGCGGCTTCGCCATCTGCGTCTCGAAGC
CCGACGGCTCGGGCGAGCGCGTGCTCACCGAGGGCTTCCACAACGAGGGCCCGACCTTCGCGCCGAACGGCCAGTACGTG
CTGTTCTTCCGCGATCCGGGCGGCCAGGGCGGCGGCAAGCTGTTCATGGTCGACATCACCGGCAAGGTGGAGCAGCCGGT
CCCGACGCCCGCCTACGCGTCCGACCCGACCTGGTCGCCGCTGCTGTCGGGCCGCTGA

Upstream 100 bases:

>100_bases
TCAACGCGGAGTTCGAGTTCACGGCGACTTGAGGGCGTCCAGCCCTTCTTCCGCCCTGATCCGGCCGCCTCGTCAGATCG
TCACTCGGATCCGACCCACC

Downstream 100 bases:

>100_bases
GCCCGTCGCGGAGCCCGGCTCGCGGGGCGGGCTCCGGGACCGTTCCCGGTCGTGGTGCAACGGTTCTGAAGGACGGCGCG
CCCCTCACCCTGTCCCTCGC

Product: beta-propeller repeat-containing to-pal system protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MLHLPSVGRLPTSFAARLIAVFTLLTAVLAGPAQAQLQLRIGGGNFQPMPIAIADFGGDPSLAGLVSGVVTNDLRRSGYF
TPLDHARFPEQPNFDAAPNFEKWRATGVQGLVTGRVVREGGRVKVEFRLWDVTNGQQMTGQQYGTDAANVRRTGHLISDG
VYTKITGLGPWFDSRIAFVDETGPKENRRKRLMVMDQDGANVRAITTGEMSIVAPRYSPATQDIAFMAQATGHQPRVQMI
NLETGSRQALGNVDSMSASPRFSPDGRKIVMSVQQGGNADIVTMDLASKAQRSITNGMAIDTSPTYSPDGSQIAFESDRG
GSQQVYVMGADGSNPHRITFGPGSASQPAFSPRGDLIAYTRQRNGGFAICVSKPDGSGERVLTEGFHNEGPTFAPNGQYV
LFFRDPGGQGGGKLFMVDITGKVEQPVPTPAYASDPTWSPLLSGR

Sequences:

>Translated_445_residues
MLHLPSVGRLPTSFAARLIAVFTLLTAVLAGPAQAQLQLRIGGGNFQPMPIAIADFGGDPSLAGLVSGVVTNDLRRSGYF
TPLDHARFPEQPNFDAAPNFEKWRATGVQGLVTGRVVREGGRVKVEFRLWDVTNGQQMTGQQYGTDAANVRRTGHLISDG
VYTKITGLGPWFDSRIAFVDETGPKENRRKRLMVMDQDGANVRAITTGEMSIVAPRYSPATQDIAFMAQATGHQPRVQMI
NLETGSRQALGNVDSMSASPRFSPDGRKIVMSVQQGGNADIVTMDLASKAQRSITNGMAIDTSPTYSPDGSQIAFESDRG
GSQQVYVMGADGSNPHRITFGPGSASQPAFSPRGDLIAYTRQRNGGFAICVSKPDGSGERVLTEGFHNEGPTFAPNGQYV
LFFRDPGGQGGGKLFMVDITGKVEQPVPTPAYASDPTWSPLLSGR
>Mature_445_residues
MLHLPSVGRLPTSFAARLIAVFTLLTAVLAGPAQAQLQLRIGGGNFQPMPIAIADFGGDPSLAGLVSGVVTNDLRRSGYF
TPLDHARFPEQPNFDAAPNFEKWRATGVQGLVTGRVVREGGRVKVEFRLWDVTNGQQMTGQQYGTDAANVRRTGHLISDG
VYTKITGLGPWFDSRIAFVDETGPKENRRKRLMVMDQDGANVRAITTGEMSIVAPRYSPATQDIAFMAQATGHQPRVQMI
NLETGSRQALGNVDSMSASPRFSPDGRKIVMSVQQGGNADIVTMDLASKAQRSITNGMAIDTSPTYSPDGSQIAFESDRG
GSQQVYVMGADGSNPHRITFGPGSASQPAFSPRGDLIAYTRQRNGGFAICVSKPDGSGERVLTEGFHNEGPTFAPNGQYV
LFFRDPGGQGGGKLFMVDITGKVEQPVPTPAYASDPTWSPLLSGR

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=439, Percent_Identity=30.9794988610478, Blast_Score=176, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR006311
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 47652; Mature: 47652

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHLPSVGRLPTSFAARLIAVFTLLTAVLAGPAQAQLQLRIGGGNFQPMPIAIADFGGDP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCCC
SLAGLVSGVVTNDLRRSGYFTPLDHARFPEQPNFDAAPNFEKWRATGVQGLVTGRVVREG
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECEEEECC
GRVKVEFRLWDVTNGQQMTGQQYGTDAANVRRTGHLISDGVYTKITGLGPWFDSRIAFVD
CEEEEEEEEEECCCCCCCCCHHCCCCHHHHHHCCCEECCCCEEEEECCCCCCCCEEEEEE
ETGPKENRRKRLMVMDQDGANVRAITTGEMSIVAPRYSPATQDIAFMAQATGHQPRVQMI
CCCCCCCCCCEEEEEECCCCCEEEEEECCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEE
NLETGSRQALGNVDSMSASPRFSPDGRKIVMSVQQGGNADIVTMDLASKAQRSITNGMAI
EECCCCCHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECHHHHHHHHHCCEEE
DTSPTYSPDGSQIAFESDRGGSQQVYVMGADGSNPHRITFGPGSASQPAFSPRGDLIAYT
CCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEE
RQRNGGFAICVSKPDGSGERVLTEGFHNEGPTFAPNGQYVLFFRDPGGQGGGKLFMVDIT
EECCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEC
GKVEQPVPTPAYASDPTWSPLLSGR
CCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLHLPSVGRLPTSFAARLIAVFTLLTAVLAGPAQAQLQLRIGGGNFQPMPIAIADFGGDP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCCC
SLAGLVSGVVTNDLRRSGYFTPLDHARFPEQPNFDAAPNFEKWRATGVQGLVTGRVVREG
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECEEEECC
GRVKVEFRLWDVTNGQQMTGQQYGTDAANVRRTGHLISDGVYTKITGLGPWFDSRIAFVD
CEEEEEEEEEECCCCCCCCCHHCCCCHHHHHHCCCEECCCCEEEEECCCCCCCCEEEEEE
ETGPKENRRKRLMVMDQDGANVRAITTGEMSIVAPRYSPATQDIAFMAQATGHQPRVQMI
CCCCCCCCCCEEEEEECCCCCEEEEEECCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEE
NLETGSRQALGNVDSMSASPRFSPDGRKIVMSVQQGGNADIVTMDLASKAQRSITNGMAI
EECCCCCHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECHHHHHHHHHCCEEE
DTSPTYSPDGSQIAFESDRGGSQQVYVMGADGSNPHRITFGPGSASQPAFSPRGDLIAYT
CCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEE
RQRNGGFAICVSKPDGSGERVLTEGFHNEGPTFAPNGQYVLFFRDPGGQGGGKLFMVDIT
EECCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEC
GKVEQPVPTPAYASDPTWSPLLSGR
CCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA