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The map label for this gene is pepT [H]

Identifier: 170683987

GI number: 170683987

Start: 1129875

End: 1131131

Strand: Reverse

Name: pepT [H]

Synonym: EcSMS35_1115

Alternate gene names: 170683987

Gene position: 1131131-1129875 (Counterclockwise)

Preceding gene: 170681636

Following gene: 170679599

Centisome position: 22.32

GC content: 46.46

Gene sequence:

>1257_bases
ATGGATATTGTCGAACGCTTTATCAATTATACAAAAATTAATACCACTACCTCACGAGAAAACGGCGCAAAAGGGATCAT
GCCTTCATCTCCTGGTCAGATGAAACTTGCGAAATTGTTGGTGAGTGAACTTGAAGCATTGGGTATGGAGGATATTATTC
TCAGAGAGAATGCTATCGTAACAGCAACACTCCCTGCAAATACTGACGAAACAATCCCCGTCGTTTCCTTTTTTGGACAT
CTGGACACCAGTGCCGAACAGACAGCCGATACAAAAGCGCAACGATTACCTTATAATGGGGGCGATCTCTGTCTGAACCC
TGAACTGAATATTTATTTGCGTGAGAGTGAATTCCCGGAGCTCAAAAATTATATTGGCGATGACCTTATAGTGACAGATG
GTACCAGCCTTCTGGGGGCCGATGATAAAGCGGCGCTGGCGGCCATAATGAACGCACTTCAATTTCTGATTTCTCATCCT
GAAATCAGGCATGGAGAAGTTAAAGTTGGGTTTGTGCCAGATGAAGAGCAGGGGTTACGGGGGGCCAAAGCCTTCGATGT
TTCAGAGTTTGGCGCAGATTTTGGCTACACTCTGGATTGCTGCGGTATTGGAGAATTTGTTTACGAAAACTGGAATGCGG
GTGATGCAGAAATTATCTTTACCGGCCAGTCTGCACACCCTATGTCAGCGAAAGGCAAGCTTAAGAACTCTCTTTTGATG
GCTCACAAATTCATTTCGATGTTGCCAGGAGGAGAAGCGCCTGAATATACAGAAGGACGCGAAGGCTATTATTGGGTGAA
ACAGTTACAGGGAAACAGTGCCAGAACTGTACTAAAACTGGATATACGAGATTTCAGCGAGGAAGGATATCACGCCCGGA
AGACATTTGTACGCCAGCTTGCAGAAAGTGCTTGTGCATTATGGGGAGAAGGAAGCGTGATCTGCCAACTGAGCGATCGG
TACGCCAATGTCTTTAACAGTCTGCAGGGAGAGGGGCATTATCCCATCGACATTGCCCTGCGAGCTTACCAACGATGTGG
TATTACTCCGACACCGGTGGCGATGCGTGGAGGATATGATGGCGCTGTTCTTTCACAAAAAGGATTACCCTGCCCGAACA
TTTTTACCGGCGCACATAATTTCCATTCTATCTATGAATATCTTCCAGTCCGTTCACTTCGGGCGGCAAGTGATGTGGTT
ATTGCCATCATTCAGGAGACATTCAATGGGTTCACCACAGGGAATCGTGAGTCATGA

Upstream 100 bases:

>100_bases
AAATGACAGCCTGAACATAGCATAAATTCACGTTCTTCAGAGTCAGAAGGTGTTAATAAACTGTCCCTAAAATCTGGAAA
ATGTTGAAGAAGGCATCTCA

Downstream 100 bases:

>100_bases
TTAAAGTTGTTATCGCGCTTCTTGTTATCGTGATAGTTGCTCGCTTAATCCTGAAAGGATATCGTGCAGAACCCGTGCTT
TTTATTGCCGGGCTTGCGCT

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MDIVERFINYTKINTTTSRENGAKGIMPSSPGQMKLAKLLVSELEALGMEDIILRENAIVTATLPANTDETIPVVSFFGH
LDTSAEQTADTKAQRLPYNGGDLCLNPELNIYLRESEFPELKNYIGDDLIVTDGTSLLGADDKAALAAIMNALQFLISHP
EIRHGEVKVGFVPDEEQGLRGAKAFDVSEFGADFGYTLDCCGIGEFVYENWNAGDAEIIFTGQSAHPMSAKGKLKNSLLM
AHKFISMLPGGEAPEYTEGREGYYWVKQLQGNSARTVLKLDIRDFSEEGYHARKTFVRQLAESACALWGEGSVICQLSDR
YANVFNSLQGEGHYPIDIALRAYQRCGITPTPVAMRGGYDGAVLSQKGLPCPNIFTGAHNFHSIYEYLPVRSLRAASDVV
IAIIQETFNGFTTGNRES

Sequences:

>Translated_418_residues
MDIVERFINYTKINTTTSRENGAKGIMPSSPGQMKLAKLLVSELEALGMEDIILRENAIVTATLPANTDETIPVVSFFGH
LDTSAEQTADTKAQRLPYNGGDLCLNPELNIYLRESEFPELKNYIGDDLIVTDGTSLLGADDKAALAAIMNALQFLISHP
EIRHGEVKVGFVPDEEQGLRGAKAFDVSEFGADFGYTLDCCGIGEFVYENWNAGDAEIIFTGQSAHPMSAKGKLKNSLLM
AHKFISMLPGGEAPEYTEGREGYYWVKQLQGNSARTVLKLDIRDFSEEGYHARKTFVRQLAESACALWGEGSVICQLSDR
YANVFNSLQGEGHYPIDIALRAYQRCGITPTPVAMRGGYDGAVLSQKGLPCPNIFTGAHNFHSIYEYLPVRSLRAASDVV
IAIIQETFNGFTTGNRES
>Mature_418_residues
MDIVERFINYTKINTTTSRENGAKGIMPSSPGQMKLAKLLVSELEALGMEDIILRENAIVTATLPANTDETIPVVSFFGH
LDTSAEQTADTKAQRLPYNGGDLCLNPELNIYLRESEFPELKNYIGDDLIVTDGTSLLGADDKAALAAIMNALQFLISHP
EIRHGEVKVGFVPDEEQGLRGAKAFDVSEFGADFGYTLDCCGIGEFVYENWNAGDAEIIFTGQSAHPMSAKGKLKNSLLM
AHKFISMLPGGEAPEYTEGREGYYWVKQLQGNSARTVLKLDIRDFSEEGYHARKTFVRQLAESACALWGEGSVICQLSDR
YANVFNSLQGEGHYPIDIALRAYQRCGITPTPVAMRGGYDGAVLSQKGLPCPNIFTGAHNFHSIYEYLPVRSLRAASDVV
IAIIQETFNGFTTGNRES

Specific function: Cleaves the N-terminal amino acid of tripeptides [H]

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family [H]

Homologues:

Organism=Escherichia coli, GI1787372, Length=408, Percent_Identity=38.2352941176471, Blast_Score=275, Evalue=5e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.11.4 [H]

Molecular weight: Translated: 45835; Mature: 45835

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIVERFINYTKINTTTSRENGAKGIMPSSPGQMKLAKLLVSELEALGMEDIILRENAIV
CCHHHHHHHHEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEECCEEE
TATLPANTDETIPVVSFFGHLDTSAEQTADTKAQRLPYNGGDLCLNPELNIYLRESEFPE
EEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEECCCEEEEEECCCCHH
LKNYIGDDLIVTDGTSLLGADDKAALAAIMNALQFLISHPEIRHGEVKVGFVPDEEQGLR
HHHHCCCCEEEECCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHCCC
GAKAFDVSEFGADFGYTLDCCGIGEFVYENWNAGDAEIIFTGQSAHPMSAKGKLKNSLLM
CCCCCCHHHHCCCCCCEEEECCCCHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHH
AHKFISMLPGGEAPEYTEGREGYYWVKQLQGNSARTVLKLDIRDFSEEGYHARKTFVRQL
HHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECHHCCCCCHHHHHHHHHHH
AESACALWGEGSVICQLSDRYANVFNSLQGEGHYPIDIALRAYQRCGITPTPVAMRGGYD
HHHHHHCCCCCCEEEEECHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCC
GAVLSQKGLPCPNIFTGAHNFHSIYEYLPVRSLRAASDVVIAIIQETFNGFTTGNRES
CCEECCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDIVERFINYTKINTTTSRENGAKGIMPSSPGQMKLAKLLVSELEALGMEDIILRENAIV
CCHHHHHHHHEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEECCEEE
TATLPANTDETIPVVSFFGHLDTSAEQTADTKAQRLPYNGGDLCLNPELNIYLRESEFPE
EEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEECCCEEEEEECCCCHH
LKNYIGDDLIVTDGTSLLGADDKAALAAIMNALQFLISHPEIRHGEVKVGFVPDEEQGLR
HHHHCCCCEEEECCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHCCC
GAKAFDVSEFGADFGYTLDCCGIGEFVYENWNAGDAEIIFTGQSAHPMSAKGKLKNSLLM
CCCCCCHHHHCCCCCCEEEECCCCHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHH
AHKFISMLPGGEAPEYTEGREGYYWVKQLQGNSARTVLKLDIRDFSEEGYHARKTFVRQL
HHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECHHCCCCCHHHHHHHHHHH
AESACALWGEGSVICQLSDRYANVFNSLQGEGHYPIDIALRAYQRCGITPTPVAMRGGYD
HHHHHHCCCCCCEEEEECHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCC
GAVLSQKGLPCPNIFTGAHNFHSIYEYLPVRSLRAASDVVIAIIQETFNGFTTGNRES
CCEECCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA