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The map label for this gene is hofN [H]

Identifier: 170683225

GI number: 170683225

Start: 3728222

End: 3728761

Strand: Reverse

Name: hofN [H]

Synonym: EcSMS35_3671

Alternate gene names: 170683225

Gene position: 3728761-3728222 (Counterclockwise)

Preceding gene: 170680610

Following gene: 170682127

Centisome position: 73.57

GC content: 54.63

Gene sequence:

>540_bases
ATGAACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGT
TGCGCCTCTGCTGCTGGCCGTCGGGATTACGCTAATAATGCGTCTGACAAGCAACGCCGAAGCACGCGTAAACGCCGTTT
TGCTTCAGGCGGAACAACAACTCGCTCGCAGCTTACAGATAACGAAACCACGTTTGCTGGAGCAACAGCAGTTACGCGAA
CAGCGTTTGCAAAGGCAGCGCCAGCGACAATTTACCCGCGACTGGCAATCTGCGCTGGAAGCACTGGCGGCGCTTTTACC
CGAGCACGCCTGGCTGACAACGATAAGCTGGCAGCAGGGAACGCTGGAGATCAAGGGGCTGACAACAAGCATTACCGCGT
TAAACGCACTAGAAACGTCACTCCGCCAGGATGCATCTTTTCATCTCAATCAGCGGGGGGCCACGCAGCAGGATGCGCAG
GGACGCTGGCAATTTGAGTATCAGTTAACAAGGAAGGTTAGCGATGGACATGCTCTTTGA

Upstream 100 bases:

>100_bases
TTGATCCCTGGGAGGCCGTTTCTGTTCGTCAGCCGCCGCTACCGCCGCCAGGTGGAGACTTTGCCATCGCGCTGGGGCTG
GCGCTAGGGAAGGCGAACTG

Downstream 100 bases:

>100_bases
CTGGTGGTTCGCCGCATCACCCCGCCTCCGCCAGCTTTGCTGGGCATTCTGGTTGATGATGTTAGTTACGCTCATTTTTC
TGTCATCGACACACCAGGAA

Product: PilN family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLIMRLTSNAEARVNAVLLQAEQQLARSLQITKPRLLEQQQLRE
QRLQRQRQRQFTRDWQSALEALAALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
GRWQFEYQLTRKVSDGHAL

Sequences:

>Translated_179_residues
MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLIMRLTSNAEARVNAVLLQAEQQLARSLQITKPRLLEQQQLRE
QRLQRQRQRQFTRDWQSALEALAALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
GRWQFEYQLTRKVSDGHAL
>Mature_179_residues
MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLIMRLTSNAEARVNAVLLQAEQQLARSLQITKPRLLEQQQLRE
QRLQRQRQRQFTRDWQSALEALAALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
GRWQFEYQLTRKVSDGHAL

Specific function: Required for the use of extracellular DNA as a nutrient [H]

COG id: COG3166

COG function: function code NU; Tfp pilus assembly protein PilN

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789796, Length=179, Percent_Identity=95.5307262569832, Blast_Score=290, Evalue=5e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007813 [H]

Pfam domain/function: PF05137 PilN [H]

EC number: NA

Molecular weight: Translated: 20782; Mature: 20782

Theoretical pI: Translated: 11.85; Mature: 11.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLIMRLTSNAEARVNAVLLQAEQQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LARSLQITKPRLLEQQQLREQRLQRQRQRQFTRDWQSALEALAALLPEHAWLTTISWQQG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCC
TLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQGRWQFEYQLTRKVSDGHAL
EEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHCCCCCCC
>Mature Secondary Structure
MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLIMRLTSNAEARVNAVLLQAEQQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LARSLQITKPRLLEQQQLREQRLQRQRQRQFTRDWQSALEALAALLPEHAWLTTISWQQG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCC
TLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQGRWQFEYQLTRKVSDGHAL
EEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]