Click here to switch to the map view.

The map label for this gene is iaaA [H]

Identifier: 170682695

GI number: 170682695

Start: 1125870

End: 1126901

Strand: Reverse

Name: iaaA [H]

Synonym: EcSMS35_1111

Alternate gene names: 170682695

Gene position: 1126901-1125870 (Counterclockwise)

Preceding gene: 170679599

Following gene: 170680879

Centisome position: 22.23

GC content: 39.83

Gene sequence:

>1032_bases
ATGAATAAAATAAAAGGTATAACAGTAATGGCAGCCTTCGCATTGTGTATAGGATATGCTCACGCTGAATCTCAAACGCC
AATACGCCTTGTAATTCATGGTGGAGCTGGCACCATAACAAAAGATACTATTACACCTGAACAAGAAAAACAATACAGAG
AAAAGTTAACAGATGCACTAAATGCTGGGTATGCTGTGCTTAATAGTGGAGGAAGTAGCCTTGATGCTGTTCAGCGTGCA
ATTAATGTTATGGAGGACTCTCCCCTCTTCAATGCTGGAAAAGGCGCTGTCTTTACTCATGATGGCAAAAACGAACTTGA
TGCTGCAATTATGGATGGAAAAACCAAAATGGCCGGAGCCGTTGCAGGAGTGACGACCATAAAAAACCCTATAAACGCTG
CTTATGCAGTTATGACTAAAAGCCCTCATGTATTAATGATCTCTAATGGTGCGGAACTATTTGCAAAAGAGCAAGGCTTA
GTTATGGTTGAGCCAGCATATTTCAAAACTGATTTCCGTTGGCAACAGCTACAGAACGCTTTAAAAGACGAAAAAATAAC
CCTTGATCATAATGGTAAATCAGCATCTCTTTTACTCCCCCCAAAGAACTACGACTATAAATATGGAACTGTTGGAGCAG
TCGCGTTGGACAAAGATGGTAATCTGGCAGCTGGAACATCAACAGGAGGTATGACAAATAAACGTTACGGACGCGTAGGT
GACTCGCCAATAATTGGAGCAGGAACCTATGCCGATAATAATACCGTTGCTGTTTCTGCTACAGGTACTGGAGAAATGTT
TATTAGAACTTCAACTGCATACAATATTGCTGCTCAGGTGAAATATAAAAATACACCATTGAAAGAAGCAGCTCAAAATG
CGCTTGATGAAGTTAAAAACATTAATGGTAGTGGTGGTGTCATTGTTCTAGATAAGAATGGTAATTATACTATGAGTTTC
AATACAGAAGGGATGTATCGTGGAACTATCGGCAACGATGGTAAAGCTATCGTATCAATTTATGAACAATAG

Upstream 100 bases:

>100_bases
AAATCGAATTAGCATAATTTTTTTTGATAGAAGTCAAATAAACATAATGGAAAAAGTTTAAATATTTATATTGTATAAAC
TTCAGACAAAAGGAGGAGTT

Downstream 100 bases:

>100_bases
ATAAAATATTTTATACATCGTCTTGCCCTTAAGGCCCATTTTAAAAGCGGGCCTTTATTTAATAAATATTCTTAACGCCT
CCTTATTTCACAGCTAAAAG

Product: asparaginase family protein

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MNKIKGITVMAAFALCIGYAHAESQTPIRLVIHGGAGTITKDTITPEQEKQYREKLTDALNAGYAVLNSGGSSLDAVQRA
INVMEDSPLFNAGKGAVFTHDGKNELDAAIMDGKTKMAGAVAGVTTIKNPINAAYAVMTKSPHVLMISNGAELFAKEQGL
VMVEPAYFKTDFRWQQLQNALKDEKITLDHNGKSASLLLPPKNYDYKYGTVGAVALDKDGNLAAGTSTGGMTNKRYGRVG
DSPIIGAGTYADNNTVAVSATGTGEMFIRTSTAYNIAAQVKYKNTPLKEAAQNALDEVKNINGSGGVIVLDKNGNYTMSF
NTEGMYRGTIGNDGKAIVSIYEQ

Sequences:

>Translated_343_residues
MNKIKGITVMAAFALCIGYAHAESQTPIRLVIHGGAGTITKDTITPEQEKQYREKLTDALNAGYAVLNSGGSSLDAVQRA
INVMEDSPLFNAGKGAVFTHDGKNELDAAIMDGKTKMAGAVAGVTTIKNPINAAYAVMTKSPHVLMISNGAELFAKEQGL
VMVEPAYFKTDFRWQQLQNALKDEKITLDHNGKSASLLLPPKNYDYKYGTVGAVALDKDGNLAAGTSTGGMTNKRYGRVG
DSPIIGAGTYADNNTVAVSATGTGEMFIRTSTAYNIAAQVKYKNTPLKEAAQNALDEVKNINGSGGVIVLDKNGNYTMSF
NTEGMYRGTIGNDGKAIVSIYEQ
>Mature_343_residues
MNKIKGITVMAAFALCIGYAHAESQTPIRLVIHGGAGTITKDTITPEQEKQYREKLTDALNAGYAVLNSGGSSLDAVQRA
INVMEDSPLFNAGKGAVFTHDGKNELDAAIMDGKTKMAGAVAGVTTIKNPINAAYAVMTKSPHVLMISNGAELFAKEQGL
VMVEPAYFKTDFRWQQLQNALKDEKITLDHNGKSASLLLPPKNYDYKYGTVGAVALDKDGNLAAGTSTGGMTNKRYGRVG
DSPIIGAGTYADNNTVAVSATGTGEMFIRTSTAYNIAAQVKYKNTPLKEAAQNALDEVKNINGSGGVIVLDKNGNYTMSF
NTEGMYRGTIGNDGKAIVSIYEQ

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=300, Percent_Identity=40, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI145275200, Length=300, Percent_Identity=40, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI98991769, Length=359, Percent_Identity=29.8050139275766, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI285002251, Length=291, Percent_Identity=29.8969072164948, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1787050, Length=299, Percent_Identity=51.8394648829431, Blast_Score=303, Evalue=9e-84,
Organism=Caenorhabditis elegans, GI17569239, Length=251, Percent_Identity=27.8884462151394, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI18921183, Length=324, Percent_Identity=33.3333333333333, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI21357893, Length=275, Percent_Identity=32, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24652135, Length=236, Percent_Identity=33.4745762711864, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI221330102, Length=236, Percent_Identity=33.4745762711864, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24655553, Length=248, Percent_Identity=28.2258064516129, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 36426; Mature: 36426

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIKGITVMAAFALCIGYAHAESQTPIRLVIHGGAGTITKDTITPEQEKQYREKLTDAL
CCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHH
NAGYAVLNSGGSSLDAVQRAINVMEDSPLFNAGKGAVFTHDGKNELDAAIMDGKTKMAGA
HCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCEEECCCCHHHHH
VAGVTTIKNPINAAYAVMTKSPHVLMISNGAELFAKEQGLVMVEPAYFKTDFRWQQLQNA
HHHHHHHCCCCCCEEEEEECCCCEEEEECCHHEEECCCCEEEECCHHCCCCCCHHHHHHH
LKDEKITLDHNGKSASLLLPPKNYDYKYGTVGAVALDKDGNLAAGTSTGGMTNKRYGRVG
HCCCEEEEECCCCEEEEEECCCCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCC
DSPIIGAGTYADNNTVAVSATGTGEMFIRTSTAYNIAAQVKYKNTPLKEAAQNALDEVKN
CCCEEECCEECCCCEEEEEECCCCEEEEEECCEEEEEEEEEECCCCHHHHHHHHHHHHHC
INGSGGVIVLDKNGNYTMSFNTEGMYRGTIGNDGKAIVSIYEQ
CCCCCCEEEEECCCCEEEEECCCCEEECCCCCCCCEEEEEECC
>Mature Secondary Structure
MNKIKGITVMAAFALCIGYAHAESQTPIRLVIHGGAGTITKDTITPEQEKQYREKLTDAL
CCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHH
NAGYAVLNSGGSSLDAVQRAINVMEDSPLFNAGKGAVFTHDGKNELDAAIMDGKTKMAGA
HCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCEEECCCCHHHHH
VAGVTTIKNPINAAYAVMTKSPHVLMISNGAELFAKEQGLVMVEPAYFKTDFRWQQLQNA
HHHHHHHCCCCCCEEEEEECCCCEEEEECCHHEEECCCCEEEECCHHCCCCCCHHHHHHH
LKDEKITLDHNGKSASLLLPPKNYDYKYGTVGAVALDKDGNLAAGTSTGGMTNKRYGRVG
HCCCEEEEECCCCEEEEEECCCCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCC
DSPIIGAGTYADNNTVAVSATGTGEMFIRTSTAYNIAAQVKYKNTPLKEAAQNALDEVKN
CCCEEECCEECCCCEEEEEECCCCEEEEEECCEEEEEEEEEECCCCHHHHHHHHHHHHHC
INGSGGVIVLDKNGNYTMSFNTEGMYRGTIGNDGKAIVSIYEQ
CCCCCCEEEEECCCCEEEEECCCCEEECCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]