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The map label for this gene is hycA [H]

Identifier: 170682463

GI number: 170682463

Start: 2925866

End: 2926327

Strand: Reverse

Name: hycA [H]

Synonym: EcSMS35_2850

Alternate gene names: 170682463

Gene position: 2926327-2925866 (Counterclockwise)

Preceding gene: 170684241

Following gene: 170684164

Centisome position: 57.74

GC content: 49.35

Gene sequence:

>462_bases
ATGACTATTTGGGAAATAAGCGAGAAAGCCGATTACATCGCACAGCGGCATCGTCGCCTACAGGACCAGTGGCACATCTA
CTGTAATTCGCTGGTTCAGGGGATCACGTTATCGAAAGCGCGCCTGCATCACGCCATGAGCTGCGCGCCGGACAAAGAAC
TCTGTTTCGTCCTTTTTGAACATTTTCGTATTTATGTCACCCTGGCGGATGGCTTTAACAGCCACACCATCGAGTATTAC
GTCGAAACAAAAGAGGGTGAAGACAAACAGTGGATTGCGCAGGCGCAACTGAGCATTGACGGCATGATTGATGGCAAGGT
CAACATCCGCGATCGCGAACAGGTTCTGGAACACTATCTCGAAAAAATCGCTGGCGTTTACGACAGCTTATATACCGCCA
TTGAAAACAATGTGCCGGTGAATTTAAGCCAACTGGTAAAGGGACAAAGCCCGGCAGCATGA

Upstream 100 bases:

>100_bases
AATTCATCTTTAGCTTAAAAATCTCTTTAATAACAATAAATTAAAAGTTGGCACAGAAAATGCTTAAAGCTGGCATCTCT
GTTAAACGGGTAACCTGACA

Downstream 100 bases:

>100_bases
GCTGAGGCTTTGCCCGTTTTGCAGGCGTTACGCCTGTTTGGGGATGGGCGTGTCGATGAGTGTCGAAAATGACATTTCAT
CGGCATGTTTTCGTCAAAAA

Product: formate hydrogenlyase regulatory protein HycA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHHAMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYY
VETKEGEDKQWIAQAQLSIDGMIDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA

Sequences:

>Translated_153_residues
MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHHAMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYY
VETKEGEDKQWIAQAQLSIDGMIDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA
>Mature_152_residues
TIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHHAMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYV
ETKEGEDKQWIAQAQLSIDGMIDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA

Specific function: Regulatory protein for the formate hydrogenlyase system. Could act by directly interacting with fhlA or by preventing the binding of fhlA to the upstream activatory sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789080, Length=153, Percent_Identity=98.6928104575163, Blast_Score=317, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021285 [H]

Pfam domain/function: PF11046 HycA_repressor [H]

EC number: NA

Molecular weight: Translated: 17671; Mature: 17540

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHHAMSCAPDKELCFVLFE
CCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
HFRIYVTLADGFNSHTIEYYVETKEGEDKQWIAQAQLSIDGMIDGKVNIRDREQVLEHYL
HHEEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHCCEEECCEECCCCHHHHHHHHH
EKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA
HHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
TIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHHAMSCAPDKELCFVLFE
CEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
HFRIYVTLADGFNSHTIEYYVETKEGEDKQWIAQAQLSIDGMIDGKVNIRDREQVLEHYL
HHEEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHCCEEECCEECCCCHHHHHHHHH
EKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA
HHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]