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The map label for this gene is paaF [C]

Identifier: 170682441

GI number: 170682441

Start: 4777945

End: 4778721

Strand: Reverse

Name: paaF [C]

Synonym: EcSMS35_4679

Alternate gene names: 170682441

Gene position: 4778721-4777945 (Counterclockwise)

Preceding gene: 170681633

Following gene: 170684002

Centisome position: 94.28

GC content: 48.65

Gene sequence:

>777_bases
ATGAGTGACCAACCCGTTCTGTTCAGCAGAGCGGCGGCTTCTTGCCGCCTGACATTGAACCGTGAAGATAAATGTCATGC
TATTAATGAAGAAATGATCGAATCTCTCGACCATTATTTAAATGAAATTGAAAACGACACCACATTACGATTAGTCGAAT
TAACGGCAACCGGCGATAAATTCTTTTGTGCAGGCGGCGATATTAAATCGTGGTCAGCCTATTCGCCATTAGATATGGGC
AGAAAATGGATTAAACGCGGCAATGACGTTTTTAATCGCCTGCGCAATTTACCTCAATTAACGGTTGCGAATCTCAACGG
TCATACCATCGGCGGTGGTATTGAACTTGCCTTGTGCTGCGATATTCGTATTGCTCGCCCCAGCGCGAAATTCTCTAATC
CGGAAGTAATGCTCGGCATGGTGCCTGGCTGGATGGGCATTGAACGCGTGCTTAATCAGGTCGGTCCGGTGGTCGGTCGC
CAGATGCTGATGCTGGGTAAACGCCTGACGGCGCAGGAAGCTCAGGCGGCGAATTTAATTGATGAAGTGGTAGAAAAAGA
ACAGGTGGAAAGCTGGATGGCGAATCAGTTAGCGCAGCTGGAAAAATGTGGTCCGGTGGCGCTTGCACATATTAAGCAAC
TGATTCTGGCACTGGAAAATAAACATGCCGATTACCCGCACCAGCTACTGGCCGGATTAATGTCCGCCACTCAGGATTGC
CAGCAGGCGACGCGCGCCTTTGCCGAAAAAAGTAGCGTGAGCTTCCATAATCAATAA

Upstream 100 bases:

>100_bases
TGAACTGGAAATATTCTCACCGTATTGGTGGCAGCAAGATATTGACAGCACACTGGATATTAGCGTCGATCGCATCGCGC
ATTATTGCTAAGGAATAATC

Downstream 100 bases:

>100_bases
GGAAATTGTATGCGTAAGATAACAACCGCCGAGGCACTGGCGGCGCAAATTCAGGACGGTGCGACTATTGCTATTAGCGG
TAACGGCGGCGGTATGGTGG

Product: enoyl-CoA hydratase/isomerase family protein

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIENDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMG
RKWIKRGNDVFNRLRNLPQLTVANLNGHTIGGGIELALCCDIRIARPSAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILALENKHADYPHQLLAGLMSATQDC
QQATRAFAEKSSVSFHNQ

Sequences:

>Translated_258_residues
MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIENDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMG
RKWIKRGNDVFNRLRNLPQLTVANLNGHTIGGGIELALCCDIRIARPSAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILALENKHADYPHQLLAGLMSATQDC
QQATRAFAEKSSVSFHNQ
>Mature_257_residues
SDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIENDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGR
KWIKRGNDVFNRLRNLPQLTVANLNGHTIGGGIELALCCDIRIARPSAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGRQ
MLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILALENKHADYPHQLLAGLMSATQDCQ
QATRAFAEKSSVSFHNQ

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=254, Percent_Identity=29.9212598425197, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI4502327, Length=255, Percent_Identity=27.4509803921569, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI20127408, Length=164, Percent_Identity=29.2682926829268, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI68989263, Length=183, Percent_Identity=30.6010928961749, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI70995211, Length=246, Percent_Identity=26.4227642276423, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI62530384, Length=177, Percent_Identity=29.3785310734463, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI37594469, Length=188, Percent_Identity=25.531914893617, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI37594471, Length=188, Percent_Identity=25.531914893617, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=28.2945736434109, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI221142681, Length=252, Percent_Identity=27.3809523809524, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790281, Length=177, Percent_Identity=28.8135593220339, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788597, Length=246, Percent_Identity=27.2357723577236, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787660, Length=249, Percent_Identity=32.5301204819277, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87082183, Length=249, Percent_Identity=25.7028112449799, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788682, Length=164, Percent_Identity=27.4390243902439, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=30.1960784313725, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=192, Percent_Identity=28.6458333333333, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=237, Percent_Identity=24.0506329113924, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=24.1106719367589, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25141363, Length=184, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=199, Percent_Identity=24.1206030150754, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=255, Percent_Identity=26.2745098039216, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144276, Length=129, Percent_Identity=30.2325581395349, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17536985, Length=220, Percent_Identity=26.3636363636364, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=129, Percent_Identity=30.2325581395349, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=129, Percent_Identity=30.2325581395349, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25144157, Length=193, Percent_Identity=27.4611398963731, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25144160, Length=193, Percent_Identity=27.4611398963731, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17558304, Length=164, Percent_Identity=26.219512195122, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=196, Percent_Identity=28.0612244897959, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI20129971, Length=254, Percent_Identity=30.7086614173228, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24653477, Length=254, Percent_Identity=30.7086614173228, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24653139, Length=249, Percent_Identity=24.4979919678715, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28571729, Length=191, Percent_Identity=25.6544502617801, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI28571730, Length=191, Percent_Identity=25.6544502617801, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24583165, Length=201, Percent_Identity=29.8507462686567, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19921018, Length=231, Percent_Identity=29.004329004329, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24654903, Length=201, Percent_Identity=24.8756218905473, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24650670, Length=220, Percent_Identity=25.4545454545455, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28615; Mature: 28484

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIENDTTLRLVELTATGDK
CCCCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCE
FFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTVANLNGHTIGGGIELALCC
EEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCEEECCCEEEEEEE
DIRIARPSAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGRQMLMLGKRLTAQEAQAANLI
EEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILALENKHADYPHQLLAGLMSATQDC
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
QQATRAFAEKSSVSFHNQ
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIENDTTLRLVELTATGDK
CCCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCE
FFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTVANLNGHTIGGGIELALCC
EEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCEEECCCEEEEEEE
DIRIARPSAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGRQMLMLGKRLTAQEAQAANLI
EEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILALENKHADYPHQLLAGLMSATQDC
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
QQATRAFAEKSSVSFHNQ
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA