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The map label for this gene is insQ [H]

Identifier: 170682393

GI number: 170682393

Start: 3308800

End: 3309999

Strand: Reverse

Name: insQ [H]

Synonym: EcSMS35_3236

Alternate gene names: 170682393

Gene position: 3309999-3308800 (Counterclockwise)

Preceding gene: 170682883

Following gene: 170681020

Centisome position: 65.31

GC content: 48.08

Gene sequence:

>1200_bases
ATGCTAATCCTGAAAGCCTACAAATTCAGACTGGAACCAACGCATGAGCAGTCGCAGCGTTTGCGGCAGTTATGTGGTTG
TGCCCGTTTTGTCTGGAATTTAGGTCTTGCGGAGACAAAGCGCATACTTGGCTCAGGCGAAAAGTTACCTTCGGCTTTCG
AGTTGAATCGGATGATTACAGTGTGGAAAAAAATGCCGGAATACATCTTCTTACAGGATGCTTATACCGACAATCTGCAA
CAAAAGCTGAAAGACCTGCATACCGCATGGAAACGTTGTTTTGATAAAAAGCTCGCAGCTAAGGCTCCGGTATGGAAACG
AAAAAATGAGGGCAGAGACTCAATCCGTTTTGTGAACTTTGAGAAATATTGCTGCCTTGAAAATCGCAGAGTGAAGCTAC
CGTCAGGTCTTGGGTGGGTAAAATTCCGGCAATCTCAACGTGTGAACGGTAAAATCAAAAATGCGACAATCAGTCAGTTA
GCGGGACAGTGGTATATCTCGTTTCAGGTTGAAATTGAAACGGCAGAACCAAATCACACAAGCACAACGATAGTCGGACT
GGATGCAGGCGTGGCTAAACTTGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAATCAGA
AGAAGCTGGCGAGACTCCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATA
CAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTTCACAAAGTCACAACGACCGTCAGCAAAAACCACGC
AATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCGGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATG
TCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGAGTACAAACAG
CTCTGGCGTGGTGGTCATGTAGAGGCGGTAAATCCGGCATACACAAGCCAGCGTTGTTCGTGTTGCGGTCATACGGAAAA
AGCAAATCGTCGCACACAAAGTAAGTTTGAGTGCAAAGCATGTGGGTATGCTGAAAATGCGGACGTAAACGCAGCACGAA
ACATTTTAGCGACGTGGCACGCTCAAATGGCTACAAGTACCGCGGGACACGCGGAAACCGGGAGTCTGTCTCTGGGATAG

Upstream 100 bases:

>100_bases
GAAGGCTGCATGTCTTGAACGATTGATTTTATATTTTTGCATTGAGGTTGGTCGTAAAAGCAGTAATATTGCAAGAAAAT
CTACTACACACTGAATGATG

Downstream 100 bases:

>100_bases
ACTTCCTACGCCTGTGGAGAGGTCGGTGCAGTAAGACCGCTCGATGAAGCAGGAACCAGACGCTGAGCAATCAGCCGTCA
CCCACTAATGGGAATCCCCC

Product: IS200 transposase orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MLILKAYKFRLEPTHEQSQRLRQLCGCARFVWNLGLAETKRILGSGEKLPSAFELNRMITVWKKMPEYIFLQDAYTDNLQ
QKLKDLHTAWKRCFDKKLAAKAPVWKRKNEGRDSIRFVNFEKYCCLENRRVKLPSGLGWVKFRQSQRVNGKIKNATISQL
AGQWYISFQVEIETAEPNHTSTTIVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKI
QRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQ
LWRGGHVEAVNPAYTSQRCSCCGHTEKANRRTQSKFECKACGYAENADVNAARNILATWHAQMATSTAGHAETGSLSLG

Sequences:

>Translated_399_residues
MLILKAYKFRLEPTHEQSQRLRQLCGCARFVWNLGLAETKRILGSGEKLPSAFELNRMITVWKKMPEYIFLQDAYTDNLQ
QKLKDLHTAWKRCFDKKLAAKAPVWKRKNEGRDSIRFVNFEKYCCLENRRVKLPSGLGWVKFRQSQRVNGKIKNATISQL
AGQWYISFQVEIETAEPNHTSTTIVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKI
QRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQ
LWRGGHVEAVNPAYTSQRCSCCGHTEKANRRTQSKFECKACGYAENADVNAARNILATWHAQMATSTAGHAETGSLSLG
>Mature_399_residues
MLILKAYKFRLEPTHEQSQRLRQLCGCARFVWNLGLAETKRILGSGEKLPSAFELNRMITVWKKMPEYIFLQDAYTDNLQ
QKLKDLHTAWKRCFDKKLAAKAPVWKRKNEGRDSIRFVNFEKYCCLENRRVKLPSGLGWVKFRQSQRVNGKIKNATISQL
AGQWYISFQVEIETAEPNHTSTTIVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKI
QRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQ
LWRGGHVEAVNPAYTSQRCSCCGHTEKANRRTQSKFECKACGYAENADVNAARNILATWHAQMATSTAGHAETGSLSLG

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG0675

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transposase 35 family [H]

Homologues:

Organism=Escherichia coli, GI288549314, Length=364, Percent_Identity=65.6593406593407, Blast_Score=462, Evalue=1e-131,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001959
- InterPro:   IPR010095
- InterPro:   IPR021027 [H]

Pfam domain/function: PF12323 HTH_14; PF01385 Transposase_2; PF07282 Transposase_35 [H]

EC number: NA

Molecular weight: Translated: 45584; Mature: 45584

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLILKAYKFRLEPTHEQSQRLRQLCGCARFVWNLGLAETKRILGSGEKLPSAFELNRMIT
CEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHH
VWKKMPEYIFLQDAYTDNLQQKLKDLHTAWKRCFDKKLAAKAPVWKRKNEGRDSIRFVNF
HHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCEEEECC
EKYCCLENRRVKLPSGLGWVKFRQSQRVNGKIKNATISQLAGQWYISFQVEIETAEPNHT
HHHEECCCCEEECCCCCCHHHHHCCCCCCCEECCHHHHHHCCCEEEEEEEEEEECCCCCC
STTIVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKI
CEEEEEECCCHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
QRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGL
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCC
NRSILDQGWYEMRRQLEYKQLWRGGHVEAVNPAYTSQRCSCCGHTEKANRRTQSKFECKA
CHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHCCCCHHHHHHHCCCCHHHH
CGYAENADVNAARNILATWHAQMATSTAGHAETGSLSLG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLILKAYKFRLEPTHEQSQRLRQLCGCARFVWNLGLAETKRILGSGEKLPSAFELNRMIT
CEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHH
VWKKMPEYIFLQDAYTDNLQQKLKDLHTAWKRCFDKKLAAKAPVWKRKNEGRDSIRFVNF
HHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCEEEECC
EKYCCLENRRVKLPSGLGWVKFRQSQRVNGKIKNATISQLAGQWYISFQVEIETAEPNHT
HHHEECCCCEEECCCCCCHHHHHCCCCCCCEECCHHHHHHCCCEEEEEEEEEEECCCCCC
STTIVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKI
CEEEEEECCCHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
QRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGL
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCC
NRSILDQGWYEMRRQLEYKQLWRGGHVEAVNPAYTSQRCSCCGHTEKANRRTQSKFECKA
CHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHCCCCHHHHHHHCCCCHHHH
CGYAENADVNAARNILATWHAQMATSTAGHAETGSLSLG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]