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The map label for this gene is ycbF [H]

Identifier: 170681882

GI number: 170681882

Start: 2182791

End: 2183480

Strand: Reverse

Name: ycbF [H]

Synonym: EcSMS35_2175

Alternate gene names: 170681882

Gene position: 2183480-2182791 (Counterclockwise)

Preceding gene: 170682280

Following gene: 170682541

Centisome position: 43.08

GC content: 50.87

Gene sequence:

>690_bases
TTGGCGCTCTTGATTTTCGCCGTTTTATCGCTGCTGGTGGCGAGTGAATTGCAGGCAGGAGTGGTGGTCGGCGGAACGCG
ATTTATCTTTCCGGCAGACAGAGATTCGATATCTATTTTATTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTA
AAATCAATCGCCCAACGCGTTGGGCTGGGGGTGAAGCGTCGACAGTACCAGCACCATTACTGGCCGCTCCGCCACTTATT
CTCCTGAAGCCCGGTACTACAGGCACATTGCGCTTGCTGAGAACGGAAAGCGACATCTTGCCCGTGGATCGCGAAACGCT
ATTTGAGTTAAGCATTGCCAGCGTGCCATCCGGCAAAGTTGAAAATCAGAGCGTAAAAGTGGCGATGCGCTCGGTATTTA
AACTGTTCTGGCGACCCGAAGGTTTGCCGGGCGACCCGCTGGAAGCTTATCAACAATTACGCTGGACACGGAATTCGCAG
GGTGTACAACTCACTAACCCAACGCCTTACTACATTAACCTGATTCAGGTGAGCGTAAATGGTAAAGCGTTAAGTAATGC
GGGAGTTGTGCCACCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCTATAACACCGTGTCATGTCTCCTGGCGGACGA
TAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGA

Upstream 100 bases:

>100_bases
TTTTACGCGCAATTGCGACCTGTCAATAGCGATGTGCAGTCGGGTAAAGTGAATGCCAGCGCGACATTTGTACTTCATTA
TGACTAATACGTGGAACAGA

Downstream 100 bases:

>100_bases
AACAGGCTCGGAAAACGTTTGCGCTTTTTTTGCCACAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCC
GGTTTGCACACCGGGAATCC

Product: putativi pili assembly chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MALLIFAVLSLLVASELQAGVVVGGTRFIFPADRDSISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLI
LLKPGTTGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPGDPLEAYQQLRWTRNSQ
GVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQAITPCHVSWRTINDYGGLSAKKEQNLP

Sequences:

>Translated_229_residues
MALLIFAVLSLLVASELQAGVVVGGTRFIFPADRDSISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLI
LLKPGTTGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPGDPLEAYQQLRWTRNSQ
GVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQAITPCHVSWRTINDYGGLSAKKEQNLP
>Mature_228_residues
ALLIFAVLSLLVASELQAGVVVGGTRFIFPADRDSISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLIL
LKPGTTGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPGDPLEAYQQLRWTRNSQG
VQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQAITPCHVSWRTINDYGGLSAKKEQNLP

Specific function: Could be required for the biogenesis of a putative fimbria [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI87081806, Length=229, Percent_Identity=96.943231441048, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1790771, Length=223, Percent_Identity=32.2869955156951, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787171, Length=205, Percent_Identity=34.6341463414634, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786743, Length=235, Percent_Identity=28.5106382978723, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786333, Length=227, Percent_Identity=30.3964757709251, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789532, Length=228, Percent_Identity=27.6315789473684, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082203, Length=216, Percent_Identity=25.9259259259259, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786936, Length=195, Percent_Identity=30.7692307692308, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1788428, Length=228, Percent_Identity=23.2456140350877, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788677, Length=176, Percent_Identity=27.8409090909091, Blast_Score=70, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 25031; Mature: 24900

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLIFAVLSLLVASELQAGVVVGGTRFIFPADRDSISILLTNTSQESWLINSKINRPTR
CHHHHHHHHHHHHHHHHCCCEEECCEEEEEECCCCCEEEEEECCCCCCEEEECCCCCCCC
WAGGEASTVPAPLLAAPPLILLKPGTTGTLRLLRTESDILPVDRETLFELSIASVPSGKV
CCCCCCCCCCCCHHHCCCEEEECCCCCCEEEEEECCCCCCCCCHHHHEEEEEECCCCCCC
ENQSVKVAMRSVFKLFWRPEGLPGDPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVN
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCEEEEEEEEEEC
GKALSNAGVVPPKSQRQTSWCQAITPCHVSWRTINDYGGLSAKKEQNLP
CCEECCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
ALLIFAVLSLLVASELQAGVVVGGTRFIFPADRDSISILLTNTSQESWLINSKINRPTR
HHHHHHHHHHHHHHHHCCCEEECCEEEEEECCCCCEEEEEECCCCCCEEEECCCCCCCC
WAGGEASTVPAPLLAAPPLILLKPGTTGTLRLLRTESDILPVDRETLFELSIASVPSGKV
CCCCCCCCCCCCHHHCCCEEEECCCCCCEEEEEECCCCCCCCCHHHHEEEEEECCCCCCC
ENQSVKVAMRSVFKLFWRPEGLPGDPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVN
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCEEEEEEEEEEC
GKALSNAGVVPPKSQRQTSWCQAITPCHVSWRTINDYGGLSAKKEQNLP
CCEECCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 2992959; 7984428 [H]