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The map label for this gene is mdtN [H]

Identifier: 170681502

GI number: 170681502

Start: 4648686

End: 4649717

Strand: Reverse

Name: mdtN [H]

Synonym: EcSMS35_4546

Alternate gene names: 170681502

Gene position: 4649717-4648686 (Counterclockwise)

Preceding gene: 170681616

Following gene: 170681173

Centisome position: 91.74

GC content: 56.3

Gene sequence:

>1032_bases
ATGGAAAGTACGCCGAAAAAAGCTCCTCGCAGTAAATTCCCTGCTCTGTTAGTGGTTGCGTTGGCGCTGGTTGCCCTTGT
TTTCGTTATCTGGCGCGTAGACAGTGCGCCATCAACTAATGACGCCTACGCGTCAGCAGATACCATTGATGTGGTGCCGG
AAGTCAGCGGTCGCATTGTAGAACTGGCGGTCACCGACAACCAGGCGGTGAAACAGGGCGATTTGCTGTTCCGCATCGAC
CCGCGCCCGTACGAAGCCAATCTGGCGAAAGCCGAAGCCTCCCTCGCGGCGCTGGATAAGCAAATTATGCTCACCCAACG
TAGCGTTGACGCGCAACAGTTTGGTGCCGACTCGGTTAATGCCACGGTAGAAAAAGCCCGTGCCGCCGCGAAACAGGCCA
CAGATACATTACGCCGCACCGAGCCATTACTGAAAGAAGGTTTTGTCTCAGCGGAAGACGTTGACCGTGCAAGAACTGCG
CAGCGCGCCGCAGAAGCCGATCTTAATGCCGTATTGTTGCAGGCGCAGTCAGCCGCCAGTGCCGTCAGCGGCGTCGATGC
GCTGGTCGCCCAACGTGCGGCGGTCGAAGCGGATATTGCCCTGACCAAACTGCATCTGGAAATGGCGACCGTTCGCGCGC
CGTTTGATGGCCGGGTCATTTCCCTCAAAACCTCCGTCGGACAATTTGCTTCTGCCATGCGCCCTATTTTTACCCTAATC
GACACTCGTCACTGGTACGTGATCGCCAACTTCCGCGAAACCGATCTGAAAAATATTCGCTCAGGTACACCCGCAACGAT
TCGCCTGATGAGCGACAGCGGCAAAACCTTCGAGGGTAAAGTGGATTCGATTGGCTACGGCGTGCTACCGGATGACGGCG
GCCTGGTGCTGGGCGGCCTGCCGAAAGTGTCTCGTTCTATTAACTGGGTCCGCGTTGCCCAGCGTTTTCCGGTCAAAATC
ATGGTTGAAAAACCTGACCCGGAAATGTTCCGCATCGGCGCTTCGGCAGTCGCTAATCTTGAGCCACAATAA

Upstream 100 bases:

>100_bases
TATCAATCTGGCATTTGTCGGAATTATTTATACCGCCCTTTTTGCTCTCTACGCCATGCTGTTCTGGCTGGCATTTTTCT
AATCTATTTGAGACGCGCTC

Downstream 100 bases:

>100_bases
TGAGCGCGCTCAACTCCCTGCCATTACCGGTGGTCAGGCTGCTGGCGTTCTTTCATGAAGAGTTAAGCGAGCGGCGGCCA
GGTCGTGTGCCGCAGACCGT

Product: multidrug resistance protein MdtN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRID
PRPYEANLAKAEASLAALDKQIMLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSVGQFASAMRPIFTLI
DTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKI
MVEKPDPEMFRIGASAVANLEPQ

Sequences:

>Translated_343_residues
MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRID
PRPYEANLAKAEASLAALDKQIMLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSVGQFASAMRPIFTLI
DTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKI
MVEKPDPEMFRIGASAVANLEPQ
>Mature_343_residues
MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRID
PRPYEANLAKAEASLAALDKQIMLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSVGQFASAMRPIFTLI
DTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKI
MVEKPDPEMFRIGASAVANLEPQ

Specific function: Could be involved in resistance to puromycin, acriflavine and tetraphenylarsonium chloride [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790519, Length=343, Percent_Identity=99.7084548104956, Blast_Score=684, Evalue=0.0,
Organism=Escherichia coli, GI87081951, Length=318, Percent_Identity=32.3899371069182, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1789637, Length=337, Percent_Identity=28.7833827893175, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788711, Length=355, Percent_Identity=27.3239436619718, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1789041, Length=333, Percent_Identity=28.8288288288288, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790012, Length=258, Percent_Identity=29.4573643410853, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790024, Length=254, Percent_Identity=27.1653543307087, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 36923; Mature: 36923

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVVPEVSGRIV
CCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCEE
ELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQIMLTQRSVDAQQFGADSVN
EEEECCCCHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHH
ATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADLNAVLLQAQSAAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHEEEHHHHHH
AVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSVGQFASAMRPIFTLI
HHHHHHHHHHHHHHHHHHHHHHHHEEEHHHEECCCCCEEEEEECCHHHHHHHHHHHHHHH
DTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGL
CCCEEEEEEECCHHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCCCEEEECC
PKVSRSINWVRVAQRFPVKIMVEKPDPEMFRIGASAVANLEPQ
HHHHHCCHHEEHHHHCCEEEEEECCCCHHEEECHHHHCCCCCC
>Mature Secondary Structure
MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVVPEVSGRIV
CCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCEE
ELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQIMLTQRSVDAQQFGADSVN
EEEECCCCHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHH
ATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADLNAVLLQAQSAAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHEEEHHHHHH
AVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSVGQFASAMRPIFTLI
HHHHHHHHHHHHHHHHHHHHHHHHEEEHHHEECCCCCEEEEEECCHHHHHHHHHHHHHHH
DTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGL
CCCEEEEEEECCHHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCCCEEEECC
PKVSRSINWVRVAQRFPVKIMVEKPDPEMFRIGASAVANLEPQ
HHHHHCCHHEEHHHHCCEEEEEECCCCHHEEECHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]